full-length spp24, but not its 18.5-kda proteolytic fragment, inhibits bone-healing in a rodent...

Full-Length spp24, but Not Its 18.5-kDa Proteolytic Fragment, Inhibits Bone-Healing in a Rodent Model of

Spine Fusion

by Chananit Sintuu, Robert J. Simon, Masashi Miyazaki, Yuichiro Morishita, Henry J. Hymanson, Cyrus Taghavi, Elsa J. Brochmann, Samuel S. Murray, and Jeffrey C.

Wang

J Bone Joint Surg AmVolume 93(11):1022-1032

June 1, 2011

©2011 by The Journal of Bone and Joint Surgery, Inc.

Bar graph showing the radiographic evidence of bone formation at the L4-L5 fusion site over time and according to the various rhBMP-2 doses given.

Chananit Sintuu et al. J Bone Joint Surg Am 2011;93:1022-1032


Bar graph showing the radiographic evidence of bone formation in samples treated with both rhBMP-2 and the recombinant isoform of spp24. a: Bone area for animals implanted with 1 μg of

rhBMP-2 plus varying doses of truncated spp18 (TR-spp18) (left) or full-...



a: Dose response of rhBMP-2-mediated bone formation, as determined by manual assessment of bone formation.



Representative microquantitative computed tomography images of spines treated with rhBMP-2 and harvested at varying time points.



Representative anterior and lateral microquantitative computed tomography images of specimens treated with truncated spp18 (TR-spp18) with or without rhBMP-2.



Representative anterior and lateral microquantitative computed tomography images of specimens treated with full-length spp24 (FL-spp24) with or without rhBMP-2.



Hematoxylin-and-eosin-stained sagittal sections of control spines.



Hematoxylin-and-eosin-stained sagittal sections of spines implanted with truncated spp18 (TR-spp18) and rhBMP-2.



Hematoxylin-and-eosin-stained sagittal sections of spines implanted with full-length spp24 (FL-spp24) and rhBMP-2.



full-length spp24, but not its 18.5-kda proteolytic fragment, inhibits bone-healing in a rodent...

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