FROM IMAGES TO ANSWERS

Portfolio de Productos de Media Cybernetics Olympus Latin America Q2 2006

New Releases for 2006

•AutoQuant X

•InVivo/InVitro 3

•Image-Pro Plus 6 (Slightly Higher Price but Lots More Stuff)

•Scope-Pro 6 (Piezo Support and More…)

•Image-Pro Analyzer 6 (Single Seat, 3-Seat)

•Image-Pro Express 6 (Lower Price; More Features)•IPE 3DS

•IPE-MC

•Scope-Pro Express 6 (Z and Shutter Control)

•Vertical Applications:•IQ-Otolith & IPP-Otolith Macro

•EDF Analyzer

•IQbase 2

New Releases

InVivo 3

Top Features in InVivo 3

•1-Button Data Set Export to Image-Pro Plus 6.0

•Yes

•Finally

•Windows XP x64 Support

•Large memory address handling

•64-bit camera drivers from QImaging and Hamamatsu

•Variable Rate Time Lapse Acquisition

•Nyquist Calculation for Z-Stack Acquisition

•Multi-Camera Support

•Support for Up To Four Concurrent Cameras

•True Simultaneous Acquisition from Two Monochrome Photometrics or QImaging Cameras

•Updated Driver Support for Other Popular Cameras

Top Features in InVivo 3

•FRET Acquisition

•Live Calibrated Ratio Display

Top Features in InVivo 3, part deux

•Stage Navigation Upgrades

•Cell Chambers and Multi-Well Plate templates

Top Features in InVivo 3, part troix

•New Microscope Control Dialog Illustrations for Leica Stands

•Support for DM6000, 5000, 4000 & DMI6000, 5000, 4000

InVivo Family Software Series

InVivo Live Cell Imaging Solution for Calcium Ratios, FRET and Other Dynamic Processes

ExpIO Vital Systems Control and Measurement

Image Renamed “InVitro” at Lower Price

Capture Discontinued

• Photometrics Family of Monochrome Cameras (including MAC, but NOT AS FAST! [no circular buffer support])

• QImaging Family of Cameras (including MAC)• Some Hamamatsu Cameras (not including Mac)• Some Stanford Photonics Cameras

Digital Camera Support

InVivo Acquisition

Camera Control

Fully Automated Microscope Control

Multi Color Acquisition

Time Lapse, Burst Acquisition

3D, z-series, Burst 3D with piezo

Tiling, Mosaic, Slide and Well Scanning

FRET Acquisition

Optical Splitter Acquisition

Multi Dimensional Acquisition

InVivo Analysis

Live Ratio and Ca++ Imaging

Intensity Calibration (Including Grynkewicz)

Vital Systems Control and Measurement

Signals and Systems control

Continuous Wavelength Imaging Devices

Live Cell Imaging

Pre-Configured Systems

• Automated Microscopes

• Digital Cameras

• Stage Automation

• Vital Systems

Live Cell Imaging

Application Solutions with InVivo Bundles

Multi Color Time Lapse Acquisition

Live Intensity and Ratio Measurements with Fast Acquisition using InVivo

Advanced Ratiometric and Intensity-based Tracking in Image-Pro Plus

Time Lapse, Burst Acquisition and Advanced Tracking

High Speed Acquisition for Small Region(s)

Track Single or Multiple Moving Particles

3D Acquisition, Deconvolution and Measurement

Multicolor Z and Burst Z Acquisition

Multi Color Deconvolution 2D & 3D Blind

Advanced 3D Analysis and Measurements

Multidimensional Acquisition and Analysis

Count Size

Extended Depth of Field

Signals and Actions Acquisition and Analysis

www.mediacy.comSilver Spring, MD (Headquarters)

Offices in:

Boston, MA, Pittsburgh, PA & San Diego, CA

Leiden, The Netherlands

Göttingen, Germany

Wokingham, United Kingdom

Singapore, Republic of Singapore

Tokyo, Japan

Shanghai, China

New Releases

Image-Pro Plus 6

Top New Features in Image-Pro 6

•Live Image Scaling for Low-Light Applications

•Live Data Collection and Charting

•XP x64 Support to Open Larger Image Sets

•Open GL 3D Viewer

•Direct Import of QED Files (File, Open)

•User-Defined Equation Editor in Data Collector

•Dynamically Linked Graphic Display in Data Collector

•Embedded I/O Control for Serial and Parallel Devices

•ICC Color Profiles

New Open GL 3D Viewer

More File Support

New Data Collector Tools

New Data Input/Output Tool

New Color Management Tool

Image-Pro 6 Enhancements

•Image-Pro Plus 6 Feature Enhancements:

•Updated Capture Dialog

•Scalable Calibration Markers

•User-Specific Menus

•“Dark Mode” for Low Light Laboratory Environments

•Automatic Measurement of >100,000 Objects (Memory-Dependent)

•Intelligent Macro Editor (not Intelli-Sense, but something much like it ;) )

•3D Convolution Filters

•More Extensive File Support (FV1000, JPEG2000, etc.)

•Export to Excel or Origin

•Generate Movies from Surface Plot

•6-Month License for IQbase 2 (Optional Permanent License at Extra Cost)

Updated Capture Dialog

New Scalable Calibration

New Menu Editor

New Dark Mode

New 3D Convolution Filters

Top New Features in Scope-Pro 6

•3-Point Predictive Focus for Wavy Samples

•Support for Simultaneous Stepper and Piezo Drives (X, Y, and Z)

•Calibrated Continuous Zoom Support for Olympus SZ-X

•Objective Paracentricity (X,Y) Offsets

•Analog/TTL Support for Shutters, Filter Wheels, AOTF’s and Monochromators

•Nyquist Calculator for Z-Axis Acquisition

•Support for the Olympus ZDC & Nikon Perfect Focus

Image-Pro Family Modules

•Automate Motorized Microscopes and Other Acquisition Devices

• A/D & D/A Acquisition & Support for External Devices

•SharpStack Plus includes: 3D Blind, 2D Blind deconvolution algorithms plus No Neighbor, Nearest Neighbor and Inverse Filter algorithms

•Turbo Scan•High Speed Tiling

•Back as a Plug-In by Popular Request

• QImaging* (Excellent)• Photometrics* (Excellent)• Leica DFC* (Excellent)• Olympus DP50 / DP70 / DP30 (Excellent)• Nikon DXM1200/F DS5 (Good)• Hamamatsu (Good, 3rd Party Driver)• Cooke PCO (Good, 3rd Party Driver)• Diagnostic Instruments (Fair, 3rd Party Driver)• Lumenera (Fair, 3rd Party Driver)

Camera Support for Image-Pro Family

* Image-Pro 6.X Support

www.mediacy.comSilver Spring, MD (Headquarters)

Offices in:

Boston, MA, Pittsburgh, PA & San Diego, CA

Leiden, The Netherlands

Göttingen, Germany

Wokingham, United Kingdom

Singapore, Republic of Singapore

Tokyo, Japan

Shanghai, China

New Releases

IQbase 2

Top New Features of IQbase 2

•IQbase 2 Features:

•Capture Module (Optional extra Cost)

•Support for Active Directory – Use Windows Identities for log-in and Security Levels

•Export Images and Data to PowerPoint

•Apply Measurements to Archived Images

•Compare Images Side-by-Side using Lightbox Tool

•Use 3D Scatterplots to Mine Data in Three Dimensions

•Speed Optimized for Analysis, Searching, Opening and Displaying

•New Manual Measurement Tools

IQbase 2 Powering Image Informatics

•Manages Images and Data•Drives Discovery •Facilitates Collaboration•Easy to Use•Works the Way You Do•Grows with Your Organization (Scalable)

IQstudioAnalytical Imaging Development Kit

Rapid Development of Image Analysis Applications

•Develop Applications Using our Tools•Add Image Analysis to Existing Products•Standardize Image Analysis within the Organization•Distribute Custom Applications with Run-Time Licensing

Deconvolution, 3D Rendering & Measurement

Deblurring vs Deconvolution

Auto-Quant® Algorithms are Used in Sharpstack

The No Neighbor & Nearest Neighbor Algorithms are Deblurring Tools (Intensities are Removed)

The Inverse Filter Algorithm is a Haze Restoration Tool (Intensities are Re-Assigned)

The Optional 2D Blind and 3D Blind Tools are Deconvolution Algorithms (Intensities are Re-Assigned)

The Sharpstack Algorithms are not Appropriate for Confocal Images Because They Use Widefield PSFs

Deblurring vs Deconvolution

AutoQuant X

AQI X Advantage

• Sequence detection– Multi time point– Multi channel

• Multi-Processing, Multi-Threaded• Best in Deconvolution

– Only Blind Algorithm Commercially Available

– Spherical Aberration Detection and Correction

Sequence Detection

• Loads Complex File Sequences• Dynamically Changes to Accommodate Naming of Current File

Multi Time Point

• Load 3D Time Series Data• Visualize, Deconvolve and Analyze as a

Single Dataset

Multi Channel

• Load any Number of Channels• Change Tints & Thresholds in Real-Time

Multi-Processing, Multi-Threading

• Each Operation Runs in its Own Thread• Deconvolve, Visualize a Dataset and Queue Another

Dataset at the Same Time• Run Two Deconvolutions Simultaneously• Status Display is a Dashboard for Activity• Takes Advantage of Dual Processor Computers

Blind Deconvolution

• Blind Deconvolution Yields the Best Results with the Least Effort

• No Need to Acquire or Maintain a Library of Point Spread Functions

• Adaptive PSF Changes with the Microscope: – Aberrations, Thermal Expansion, Noise,

Alignment, and Specimen are Corrected• Maximum Likelihood Expectation Models

Noise Instead of Smoothing it

Hierarchy of Algorithms

• 3D Blind Deconvolution – Premier• 2D Blind Deconvolution – Live cell• 3D Non-Blind - Faster for Derived

PSFs• 3D Non-Blind (Gold’s) – Fast but

Noisy• 3D Inverse Filter, Nearest/No

Neighbor – Quick and Dirty

Spherical Aberration

• Common Problem with RI Mismatches or Thick Samples

• Detect Using Iterative MLE or Calibrate• Pre-Bias PSF Improves with Blind

Deconvolution• Improves Signal Due to Smearing

Deconvolution of Widefield and Confocal Images

Primer on The Growing Role of Deconvolution…

Why Use Deconvolution?

To Improve Signal to Noise Ratio

What is Deconvolution?

Widefield – Single Channel

Multiple Wavelengths - WidefieldMultiple Wavelengths - Widefield

Confocal MicroscopyConfocal Microscopy

2D Blind Deconvolution2D Blind Deconvolution

2D Blind Deconvolution – TIRF2D Blind Deconvolution – TIRF

When to Use Deconvolution?

In Widefield Fluorescent Microscopy– Improve contrast of 2D and 3D Images– Improve Resolution in Three Dimensions– Improve 3D Rendering and Visualization

When to Use Deconvolution?

In Confocal Microscopy– Improve Signal to Noise Ratios– Increase Dynamic Range– Improve Image Grayscale

Resolution– Increase Axial Resolution

When to Use Deconvolution?

Documentation– Images and Projections will Show

More Detail and Greater Contrast– Improvements can be Striking

When to Use Deconvolution?

Analysis– Deconvolution Retains Quantitative

Accuracy– Improves Contrast Facilitating Analysis

• Counting • Tracking• Area Measurements• Intensity Measurements

Why Does This Work?

The Microscope is a Well-Understood Optical System

The Behavior of Light is Well-Modeled

Persistence

Why this works (1/5)Why this works (1/5)

The Point Spread of a Microscope

• Every Microscope-Sample Combination has a Different Point Spread Function

• Every Element in the Optical Path Affects the Point Spread Function:

– Scope– Objective– Cover slip– Sample

The Point Spread of a Microscope

Compare a widefield PSF to a Confocal PSF

Looking Forward to Deconvolution

YFP Labeled Motor Neurons (Mouse)

Laura Baylor, Harvard UniversityLaboratory of Dr. Jeff Lichtman

Emissive WavelengthEmissive Wavelength

Numerical ApertureNumerical Aperture

Spherical AberrationSpherical Aberration

Non-Blind deconvolution estimates the original object from the measured PSF and the collected data (2D or

3D image).

Non-Blind Deconvolution

Blind deconvolution separates the original object and the point spread from the collected data (2D or 3D

image).

Blind deconvolution

3D User Interface

3D User Tools

•Pointer to Toggle between Measurements & Volume Display

•Pan Tool to Adjust 3D Volume Position

•Home Position

•Set New Home Position

•Change Perspective (View)

•Display Box Around Volume

•Change View Y,Z or Z,X or Y,X

•Use Control Click for LR or UD, Click for UD, Alt Click to Toggle Between Pan and Pointer

3D User Options

3D Rendering with 3D Filters

3D Volume Measurements

3D Volume Measurements Over Time

3D Branch / End Point Measurements

www.mediacy.comSilver Spring, MD (Headquarters)

Worldwide Offices in:

Boston, Pittsburgh & San Diego

Leiden, The Netherlands

Goettingen, Germany

Wokingham, United Kingdom

Singapore, Republic of Singapore

Tokyo, Japan

Shanghai, China