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ab freezing bath temperaturesab freezing bath temperatureseezing mixtures in the lab are often made from a mixture of either liquid nitrogen or dry ice with

solvent. If using liquid nitrogen this forms a slurry. The viscosity of this slurry is dependent on

e solvent or mixture of solvents used. If using dry ice, you generally end up with an equilibrium

here a small amount of dry ice is sat in a cold bath of solvent. By varying the solvent used,

fferent temperatures can be achieved.

here has been lots of good work done in the past in determing the temperatures of these baths

nd there are links for further reading in the referencesbelow.

portant combinations in a biochemistry laboratory are highlighted.

Mixture Temperature (C)

p-Xylene/Liquid nitrogen 13

p-Dioxane/Liquid nitrogen 12

Cyclohexane/Liquid nitrogen 6

Benzene/Liquid nitrogen 5

Formamide/Liquid nitrogen 2

Aniline/Liquid nitrogen -6

Cycloheptane/Liquid nitrogen -12

Benzonitrile/Liquid nitrogen -13

Ethylene glycol/Dry ice -15

o-Dichlorobenzene/Liquid nitrogen -18

Tetrachloroetane/Liquid nitrogen -22

Carbon tetrachloride/Liquid nitrogen -23

Carbon tetrachloride/Dry ice -23

m-Dichlorobenzene/Liquid nitrogen -25

Nitromethane/Liquid nitrogen -29

o-X lene/Li uid nitro en -29

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Bromobenzene/Liquid nitrogen -30

Iodobenzene/Liquid nitrogen -31

Thiophene/Liquid nitrogen -38

3-Heptanone/Dry ice -38

Acetonitrile/Liquid nitrogen -41

Pyridine/Liquid nitrogen -42

Acetonenitrile/Dry ice -42

Chlorobenzene/Liquid nitrogen -45

Cylcohexanone/Dry ice -46

m-Xylene/Liquid nitrogen -47

n-Butyl amine/Liquid nitrogen -50

Diethyl carbitol/Dry ice -52

n-Octane/Liquid nitrogen -56

Chloroform/Dry ice -61

Chloroform/Liquid nitrogen -63

Methyl iodide/Liquid nitrogen -66

Carbitol acetate/Dry ice -67

t-Butyl amine/Liquid nitrogen -68

Ethanol/Dry ice -72

Trichloroethylene/Liquid nitrogen -73

Butyl acetate/Liquid nitrogen -77

Acetone/Dry ice -78

Isopropanol/Dry ice -78

Isoamyl acetate/Liquid nitrogen -79

Acylonitrile/Liquid nitrogen -82

Sulfur dioxide/Dry ice -82

Ethyl acetate/Liquid nitrogen -84

Ethyl methyl ketone/Liquid nitrogen -86

Acrolein/Liquid nitrogen -88

Nitroethane/Liquid nitrogen -90

Heptane/Liquid nitrogen -91

Cyclopentane/Liquid nitrogen -93

Hexane/Liquid nitrogen -94

Toluene/Liquid nitrogen -95

Methanol/Liquid nitrogen -98

Diethyl ether/Dry ice -100

n-Propyl iodide/Liquid nitrogen -101

n-Butyl iodide/Liquid nitrogen -103

Cyclohexane/Liquid nitrogen -104


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First published on 9th November 2010 and last modified on 25th June 2

-

Ethyl iodide/Liquid nitrogen -109

Carbon disulfide/Liquid nitrogen -110

Butyl bromide/Liquid nitrogen -112

Ethyl bromide/Liquid nitrogen -119

Acetaldehyde/Liquid nitrogen -124

Methyl cyclohexane/Liquid nitrogen -126

n-Pentane/Liquid nitrogen -131

1,5-Hexadiene/Liquid nitrogen -141

Isopentane/Liquid nitrogen -160

ooling baths.pdf (below) contains the original Rondeau paper cited below along with other information regarding making

oling baths with liquid nitrogen, dry ice and salt solutions.

Download Cooling baths.pdf (1.04 MB)

eferences

tp://es.ucsc.edu/~silab/CoolingBathComposition.php

ondeau, R.E. (1966) Slush baths.J. Chem. Eng. Data, 11: 124.

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