fp 8000 spectrofluorometers · fp‐8300 bio‐analysis is the most common application for...
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FP‐8000 SeriesSpectrofluorometers
FPFP‐‐8000 New Functions and Features8000 New Functions and Features
• High S/N• Wide dynamic range • High scan speed• Improved wavelength resolution • Higher order diffraction cut filter• Improved NIR performance • Improved spectral correction • Enhanced phosphorescence functions • Non‐contact IQ accessory• USB communication• Spectra Manager II & CFR• iRM‐900 • Updated accessories
FPFP‐‐86008600
Carbon nano‐tubes, NIR fluorescent dyes, up‐conversion of fluorescent glasses, etc. require NIR analysis methods.JASCO has optimized the instrument performance and offers new functions to meet expanding NIR analysis applications.
•NIR model for evaluation of new materials(Ex200nm~850nm, Em200nm~1010nm)
FPFP‐‐85008500
Analysis of white LEDs, organic EL compounds and other complex fluorescent compounds requires fluorescence instruments with advanced specifications.JASCO has enhanced the instrument functionality to meet these application requirements.
•Highest sensitivity
(>1200 RMS peak, >5000 RMS baseline)
•Highest scan speed
(Max: 60,000 nm/min)
•Dynamic range of 6.5 orders of magnitude
•Reduction of higher order diffraction peaks
FPFP‐‐83008300
Bio‐analysis is the most common application for fluorescence analysis. An optimized design enhances the performance of the FP‐8300 for integration with dedicated bio‐analysis accessories such as a microplate reader, stopped flow accessory, fluorescence anisotropy and auto‐titration capabilities.
• Functionality for bio‐applications
• Wide dynamic range over 6 orders of magnitude
• Tools for solid samples
(Phosphorescence, integrating sphere)
• High sensitivity (>680 RMS peak, >2800 RMS base)
FPFP‐‐82008200
The FP‐8200 offers essential capabilities including Auto Gain, Auto SCS and the cut filter assembly for simple, quick and reliable analyses. The FP‐8200 also includes sensitivity, scan speed and resolution enhancements.
•Wide dynamic range over 6 orders of magnitude
•Reduction of higher order diffraction peaks (option)
•High sensitivity (>380 RMS peak, >1600 RMS base)
• Advanced ADC enables rapid sampling at the rate of 0.2 msec for emission data, and new high speed signal processing by FPGA contributes to considerable improvement of the efficiency for signal acquisition
• P‐P S/N is calculated according to JIS K0120
S/N Performance S/N Performance
1780
1840
1800
1820
0 102 4 6 8
Int.
Time [sec]
Signal = 1910 Peak to Peak 351.9RMS 1534.1
Water Raman [Peak Signal]/[Peak Noise], Ex 350nmFP‐8500: >1200 (RMS), >300 (p‐p )
S/N PerformanceS/N Performance
Water Raman [Peak Signal]/[Baseline Noise], Ex 350nmFP‐8500:>5000 (RMS)
• Noise evaluated at baseline (450nm) is much smaller than at the Raman peak.
• Noise is roughly proportional to signal intensity.
RMS Noise = 0.2257Signal = 1910S/N = 8463
-100
600
0
200
400
300 750400 500 600 700
Int.
Wavelength [nm]
0
1.2
0.5
1
520 600540 560 580
Int.
Wavelength [nm]
AutoGain ONAutoGain OFF
Expansion of the foot of the small peak
380
520
400
450
500
661 665662 663 664
Int.
Wavelength [nm]
0
2400
1000
2000
600 700620 640 660 680
Int.
Wavelength [nm]
Expansion
A single spectrum collection can provide reliable acquisition of a very small peak together with a coexisting large peak. This capability is necessary for the determination of fluorescence quantum yield.
Noise Reduction Noise Reduction –– AutoGainAutoGain
AutoGain ONAutoGain OFF
Auto‐SCS (Sensitivity Control System) allows measurement from sub‐picomol to micro‐mol, without manually changing the instrument “sensitivity” setting
5×10‐13~1.8×10‐6 mol/LLinearity over 6.5 orders of magnitude
log(Y) = A × log(X) + BA = 1.023B = 9.855C.C. = 0.9999Std.Err.= 5.585E‐008
5×10‐13~1.5×10‐6 mol/LLinearity over 6 orders of magnitude
log(Y) = A × log(X) + BA = 1.007B = 9.850C.C. = 0.9999Std.Err. = 3.469E‐008
Expansion of Fluorescence Dynamic RangeExpansion of Fluorescence Dynamic Range
FP‐8200 Fluorescein
Conc.
FP‐8500 Fluorescein
Conc.
0.01 0.1 1
log I
0.0001 0.001 0.01
log Ix100x100
0.0001 0.01
log I
1 100
1 10 100
log I
1 10 100
Expansion of Fluorescence Dynamic RangeExpansion of Fluorescence Dynamic Range
Improvement in Scan SpeedImprovement in Scan Speed
FP‐8200
FP‐8300
FP‐8500
FP‐8600
20,000nm/min
20,000nm/min
60,000nm/min
Ex: 60,000nm/min
Em: 120,000nm/min
Faster is absolutely better!
Fluorescence instruments have always suffered from higher order diffraction effects.
Higher Order Diffraction Cut FiltersHigher Order Diffraction Cut Filters
Filter OFF
Filter ON
Em Spectrum of Orange plateEx spectrum of anthracene
• The new ‘cut filter’ capability removes peaks originating from higher order diffracted light to provide simple, reliable spectral acquisition and analysis.
• The cut filter system also optimized data for quantum yield determination by providing measurements of accurate spectra.
Higher Order Diffraction Cut FiltersHigher Order Diffraction Cut Filters
Without cut filtersWith cut filters
Enhancements for Phosphorescence Enhancements for Phosphorescence
Organic Electro‐Luminescence (EL) compounds are new phosphorescent materials. To analyze this type of sample, a rotating chopper mechanism is incorporated to enable measurements of phosphorescent materials with a lifetime shorterthan 1 msec.
Rotation frequency can be selected from 1200, 600, 300, 150 or 75 rpmRotating chopper allows quick phosphorescence spectra measurement Expands phosphorescence measurements to many modes: ([Spectra], [Quantitative Calibration]/[Analysis], [Fixed wavelength], [Time course], [Phosphorescence Lifetime])
Phosphorescence lifetime of Benzophenone
Range : 27.4 ‐ 43.3 msecLife time : 5.40 msecStd. err. : 0.001799Regression: Y = ‐0.185028 ×X + 11.9925C.C. : ‐0.9996
Enhancements for Phosphorescence Enhancements for Phosphorescence MeasurementsMeasurements
Fluorescence of Benzene
Phosphorescence of Benzene
IQ AccessoriesIQ Accessories
RFID accessory recognition
AccessoriesAccessories
Wide range of instrument interfaces for a variety of sample analyses requirements
StoppedStopped‐‐flow Experiments flow Experiments
Denaturation of Cytochrome C (0.5mg/mL) by 0.1N sulfuric acid. Mixing ratio of 1:1.
position
0
0.02
0.04
0.06
0.08
0.1
0.12
0.14
0.16
0.18
0.2
0 5 10 15 20 25
T‐shape (APP)
Ball Mixer (Bio‐Logic)
New Mixer (Jasco)
Entranceof the cell
Exit of the cell
SDposition
Mixing Efficiency
Calc. S.D.of ratio
entrance
exit
Stopped flow experiments are widely applied to the study of protein denaturation, enzyme reaction, and other analyses. The combination of a rapid and sensitive fluorescence spectrophotometer and a stopped flow unit provides an optimized analysis system.
FMPFMP‐‐825 Automated Microplate Reader825 Automated Microplate Reader
Faster measurements are obtained by precise positioning of the micro‐well plate in addition to rapid data acquisition.
Spectra Manager Version 2/CFRSpectra Manager Version 2/CFR
SM Ver. 2/CFRCross‐platform software
iRMiRM‐‐900900
Dedicated instrument control/analysis interface for compact systems