FORGED UNDER THE SUNMICROBES AND PHOTORECEPTORS FROM EXTREME ANDEAN PUNA

Dra. Virginia H. AlbarracínInvestigadora Independiente CONICET

Responsable Científica CIME-UNT-CONICET

Outline

HAALPoli-Extremophiles

biodiversity UV-metaresitome

The HAAL

L. Verde

Tolar Grande

Antofalla

L. SocompaAlbarracin et al., 2016, Photochemistry and Photobiology

HAAL: a highly solar irradiatedenvironment

Mean daily total solar irradiance (in W/m2) and Local Noon Time UV Index in the period 2005-2011 for all the Earth (right image)

and on the Puna of Atacama region (left image). The highest mean value of all the Earth in the range of (308 ± 13) W/m2. Mean UV

index value of 13.5± 1.0. Adapted from NASA OMI/Aura Online Visualization and Analysis (Daily Level 3 Global Gridded Products)

http://gdata1.sci.gsfc.nasa.gov/.Albarracin et al., 2015, Frontiers in Microbiology

HAAL

microbiodiversity

Microbial communities

from Bacterioplankton

Microbial communities

from the Benthos/soil

Microbial communities fromFlamingo faeces

Microbial mat communities

EMAMs

Albarracin et al., 2016, Photochemistry and Photobiology

UV-RESISTANT ACTINOBACTERIA

Rasuk et al., 2017, Photochemistry and Photobiology

HAAL resistome

High UV irradiation(among other extremes)

High microbiodiversity(microecosystem, species and gene levels)

HAAL UV-resistome

UV-Resistome

Sensing and response regulators

Efficient photoprotection

Pigments, Extremolytes or

other metabolites

Efficient stress response/energy

administration

High tolerance to damage

Efficient repairingsystems for DNA

damage?

PR

Photolyases

DR

NER and others

Photoreceptors

Light

Sensing

Response

ResistanceMechanisms

Metabolites

Comunication

Model ExtremophilesPhysiology/Multi-omics

Extremo-enzymesExtremolytes

MicroecosystemMetagenomes

UV-Resistome

Approaches

“Model extremophiles”

Lake Verde (4,400 m)

Catamarca

Maximum UV-B 10.8 W/m2

As: 0.8 ppm

P: 0.012 ppm

Salinity: 5%

Acinetobacter sp. Ver3

Model UV-resistant Acinetobacter sp. Ver3

Ver3 UV-Resistome: Genomics

Kurth et al., 2015, Frontiers in Microbiology

Ver3 UV-resistome: Proteomics

0

0.2

0.4

0.6

0.8

1

1.2

1.4

0 5 10 15 20

Spot N Proteomic change Control/UV

02-Pyridine nucleotide disulfide oxidoreductase

04- Diaminobutirate-2-oxoglutarate aminotransferase

14-Dihidroxy acid dehydratase

15- Acetyl CoA hydrolase

23- Histidyl RNAt synthetase

33- Electron transfer flavoprotein sub-beta

35- Catalase

37- Fatty acid oxidation complex sub-alpha

40- Hypotetical protein

163 differential spots

25 significantly different

ROS scavengingQuorum sensing regulatorAA and protein synthesis

Beta oxidation of fatty acidsGlycolisis

Electron transfer chain

Kurth et al., 2015, Frontiers in Microbiology

Ver3 UV-resistome: Ultrastructuromics

Galvan et al., 2018, en preparación

Mag. 20.00 kx - Escala: 200 nm Mag. 50.00 kx - Escala: 100 nmMag. 10.00 kx - Escala: 500 nm

Ver3 UV-resistome: Ultrastructuromics

Galvan et al., 2018, en preparaciónMag. 50.00 kx - Escala: 100 nmMag. 10.00 kx - Escala: 500 nmMag. 4.00 kx - Escala: 1 µm

Ver3 UV-resistome: Ultrastructuromics

Mag. 10.00 kx - Escala: 500 nm Mag. 20.00 kx - Escala: 200 nmMag. 2.00 kx - Escala: 2µm

Ver3 UV-resistome: Ultrastructuromics

Mag. 2.00 kx - Escala: 2 µm Mag. 10.00 kx - Escala: 500 nm Mag. 20.00 kx - Escala: 200 nm

Dra. Virginia AlbarracínGroup Leader

Investigadora Independiente

The A-Team-Laboratorio de Microbiología Ultraestructural y Molecular

-Laboratorio de Microscopía Molecular

Lic

Lic

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Deutscher Akademischer Austauschdienst

Max-Planck Society

Marie Curie Actions (IIF), FP7, UE

Argentinean Chapter MCAAEuropean Research Council