for the marine mammals and sea turtles working group of ...jul 06, 2011 · finley^ kym walsh^...

Nearshore Cetacean and Sea Turtle Prey Item Sam pling Plan

NEARSHORE CETACEAN & SEA TURTLE PREY ITEM SAMPLING PLAN

Prepared by:

The Prey Species In fo rm ation Subgroup Lance G arrison^ Jenny L ltz \ Ruth Carmichael^, Terl Rowles^, Tracy C ollie r^ M andy T um lln^ Heather

Finley^ Kym W alsh^ Robert P. Bourgeois®, Jason Adrlance®, Paul Lang®, Danya Machnes^

For the Marine Mammals and Sea Turtles W orking Group o f the Mississippi Canyon 252 Trustees

Version 3.0 July 6, 2011

National Oceanic and Atmospheric Administration (NCAA) National Marine Fisheries Service (NMFS) Southeast Fisheries Science Center (SEFSC) Marine Mammal Program University of South Alabama Senior Marine Scientist, Dauphin Island Sea Lab (DISL)

® NCAA NMFS Office o f Protected Resources (OPR) Marine Mammal Health and Stranding Program University Corporation for Atmospheric Research (UCAR)

® Louisiana Department o f W ildlife and Fisheries (LDWF)® Louisiana ON Spill Coordinator's Office (LOSCO) Industrial Economics, Inc.

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Nearshore Cetacean and Sea Turtle Prey Item Sampling Plan

NEARSHORE CETACEAN & SEA TURTLE PREY ITEM SAMPLING PLAN

Approval of this work plan is forthe purposes of obtaining data for the Natural Resource Damage Assessment (NRDA). Each party reserves its rights to produce its own independent interpretations and analyses of any data collected pursuant to this work plan.

The trustees have developed a preliminary conceptual model of the DWH release, potential pathways and routes of exposure, and potential receptors. This preliminary model has informed the trustees' decision to pursue the studies outlined in the work plan. By signing this work plan and agreeing to fund the work outlined, BP is not endorsing the model articulated in the work plan.

This plan will be implemented consistent with existing trustee regulations and policies. All applicable state and federal permits will be obtained prior to conducting work.

APPROVED:

i / f/lOAA Trustee Representative: Date

rustee Representative:

BP Representative: \ Date

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SummaryThis docum ent presents an assessment plan fo r prey Items o f nearshore cetaceans and sea tu rtle s In Louisiana, w here hab ita t has been Im pacted by Mississippi Canyon 252 (MG 252) oil and dispersants, (he rea fte r re ferred to as "M G 252 o i l " ) , as confirm ed by Louisiana D epartm ent o f W ild life and Fisheries (LDWF) fie ld observations (LDWF, personal com m unication). The data co llection described In th is plan ta rge ts ephem eral data and can be reasonably expected to support a natural resource damage assessment under the Oil Pollu tion Act.

ObjectiveTo obta in representative samples o f prey species po ten tia lly consumed by nearshore cetaceans and sea tu rtle s th a t are know n to inhab it th e geographic area o f in te rest over a grad ien t o f po ten tia l MG 252 oil Impacts.

The Plan specifically addresses th e fo llo w in g topics:

I. Approach and rationale. This section describes the overall purpose o f and need fo r a prey sam pling plan as a w ay to evaluate th e po ten tia l fo r nearshore cetacean and sea tu r tle exposure to MG 252 oil via th e tro p h ic trans fe r exposure pathway. This plan describes m on th ly surveys to be conducted fo r approxim ate ly 10 m onths, beginning upon signature o f th is plan.

II. Data needs and sources. This section provides an overview o f th e types o f data th a t w ill be collected to fu r th e r the assessment o f nearshore cetacean and sea tu r t le prey species In the no rthe rn G ulf o f Mexico.

III. Health and safety. This section summarizes health and safety p ro toco ls th a t are re levant to th is e ffo rt. It Includes a num ber o f procedures by reference, all o f w hich should be carefu lly reviewed and adhered to by all fie ld team members.

IV. Site selection strategy. This section describes th e approach fo r iden tify ing sites fo r evaluation.

V. Site procedures. This section provides guidance on how to handle th e samples and data gathered In th e fie ld . This section makes fre q u e n t reference to protoco ls in A ppendix I and also to NRDA-wlde procedures th a t are Incorporated by reference. Field team mem bers should make sure they understand and adhere to procedures, w h e th e r Included here d irec tly o r by reference.

Appendix I: Detailed Standard Operating Procedures (SOPs) and Field Data Sheets. This sectionw ill be com piled as SOPs and fie ld data sheets become available. P re lim inary d ra ft SOPs w ill be shared w ith BP or Its representa tive before th is plan is signed.

Appendix II: Protected Species M itiga tion Measures (Protected Resources, NOAA NMFS Southeast Regional O ffice)

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I. Approach and RationaleConsum ption o f prey contam inated w ith MG 252 oil (exposure via tro p h ic transfe r) could po ten tia lly cause In jury to e ithe r o r both nearshore cetaceans and sea tu rtle s In the Gulf o f M exico (Peterson e t al.,2003). M ore specifically, w ith in th e geographic scope o f th is plan, bo ttlenose do lphins (Turslops truncatus), Kemp's rid ley (Lepldochelys kem pll) and loggerhead (Caretta care tta ) sea tu rtle s consume prey Items th a t may have been exposed to MG 252 oil. To help de te rm ine w h e th e r exposure has occurred, samples o f th e prey Items w ill be collected fo r con tam inan t analyses, to be addressed In an addendum to th is plan. The T rustees shall p rov ide a d ra ft o f th e co o pe ra tive ana ly tica l addendum to BP by A ugust 15, 2011. BP and th e T rustees agree to w o rk to g e th e r In good fa ith to co o p e ra tive ly deve lop th is addendum and agree th a t th e addendum w ill Include analyses fo r Polycycllc A ro m a tic H ydrocarbons (PAHs) and fin g e rp r in tin g fo r M G252 o il, w he re te ch n ica lly p rac ticab le , and a schedu le o f d e live ry fo r data resu lting fro m th e proposed analyses w ith in a reasonable tim e fra m e . H ow ever, If BP and th e Trustees are unab le to reach consensus on any ind iv idua l e lem en t(s ) o f th e ana ly tica l addendum , th e T rustees reserve th e r ig h t to proceed Ind e p e n d e n tly on those e lem en ts on w h ich no a g re e m en t w as reached. If th e Trustees and BP have n o t en te red In to a coopera tive ana ly tica l addendum by O c to b e r 1, 2011, upon request o f BP, sam ples w ill be s p lit If p racticab le and each P arty m ay proceed Independen tly .

This study w ill a tte m p t to de te rm ine levels o f prey exposure to MG 252 oil by sampling key prey Items In Inshore and nearshore Louisiana w aters (no fu r th e r ou t than the 10-m eter Isobath). Known prey Items w ill be ta rgeted fo r co llection using traw ls and gill nets. This study plan has been reviewed by NOAA pro tected resources sta ff In th e NOAA NMFS Southeast Regional O ffice to ensure th a t appropria te m itiga tion measures are taken. This rev iew resulted In th e Iden tifica tion o f p reventive measures to help avoid m arine m am m al o r sea tu r t le In teractions during scheduled NDRA sampling. Based on th is review and subsequent fo llo w -u p conference calls w ith NOAA NMFS staff, th e sampling team s w ill undertake measures to help avoid m arine m am m al and sea tu r tle In teractions as noted In th e Protected Species M itiga tion Measures docum ent In Appendix II.

BP or Its representative w ill be provided an o p p o rtu n ity to partic ipa te In all sampling events described In th is plan.

II. Data needs and sourcesTable 1 provides a list o f typ ica l prey Items fo r bo ttlenose dolphins, Kemp's rid ley and loggerhead sea tu rtle s (Barros & Odell, 1990; Bjorndal, 1996; Barros & W ells, 1998; Maze-Foley & M u llln 2006; etc.). Field team s w ill a tte m p t to co llect up to 2 Independent samples o f any ta rge ted species listed In Table 1 In each net haul o r tra w l a t each sam pling sta tion (see Section V fo r site procedures). Each o f these samples should weigh approxim ate ly 30 g to provide adequate m ateria l fo r fu tu re analyses. A spring scale w ill be utilized by fie ld sampling team s to w eigh samples. If ind ividual organisms w ith in a species w eigh less than 30 g, fie ld team s w ill co llect enough Individuals to ob ta in tw o samples o f at least 30 g each. Each haul w ill be sorted to species, and individuals w ill be selected at random fro m th e sorted species un til tw o 30 gram samples have been collected fo r each species. If Individual organisms w eigh > 30 g, fie ld sampling team s w ill co llect 2 Individuals and prepare each as a separate sample. Samples o f muscle tissue (approxim ate ly 30 g each fro m 2 Individuals) w ill be taken fro m fish th a t are > 600 mm in length (see Appendix 1 fo r deta iled SOP).

A lthough n o t proposed as part o f the cu rren t scope o f th e w o rk In th is w o rk plan, sam pling and p o ten tia l analyses o f liver and bile samples w ill be addressed separately by the Trustees.

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Table 1: Typical prey Items fo r bo ttlenose dolphins, Kemp's, and loggerhead sea tu rtle s (Barros & Odell, 1990; Bjorndal, 1996; Barros & W ells, 1998; Maze-Foley & M u llln 2006; etc.).

Dolphin Prey Sea Turtle Prey

Common name Scientific name Common name Scientific name

A tlan tic croaker M Icropogonlas undulatus blue crab Callinectes sap id us

bay anchovy Anchoa m itch llll lesser blue crab Calllnectes s im ilus

G ulf menhaden Brevoortia patronus speckled sw im m ing crab Arenaeus cribrarius

pinflsh Lagodon rhom boldes Iridescent sw im m ing crab Portunus gibbesli

spot Lelostomus xanthurus Florida lady crab Ovallpes flo rldanus

striped m u lle t M u g il cephalus calico box crab Flepatus ephelltlcus

brow n shrim p Farfantepenaeus aztecus flam e box crab Calappa flam m ea

p ink shrim p Farfan tepenaeus

duorarum

ye llow box crab Calappa sulcata

w h ite shrim p Litopenaeus setlferus m o ttle d purse crab Persephona m editerranea

A tlan tic b rie f squid Lolllguncula brevis p o rtly spider crab Libinia em arg lna ta

G ulf toadflsh Opsanus beta incongruous ark A nadara braslliana

hardhead catfish Arlopsis fe lls blood ark A nadara avails

sand seatrou t Cynoscion arenarlus A tlan tic cutlassflsh Trichiurus lepturus

silver seatrou t Cynoscion nothus horseshoe crab Limulus polyphem us

spotted seatrou t Cynoscion nebulosus

striped anchovy Anchoa hepsetus

black drum Pogonlas crom is

gafftopsa ll catfish Bagre m arinas

red drum Sclaenops ocellatus

southern flounde r Parallchthys le thostlgm a

III. Health and Safety• The team leader and fie ld crew shall have com pleted all applicable health and safety

tra in ing as d irected by NOAA o r state agency oil spill policy.

• All fie ld team m em bers m ust com ple te the NOAA safety tra in ing and docum enta tion requ irem ents as set fo rth In "Safety Requirem ents fo r All Personnel W ork ing on NOAA led NRDA team s fo r MS Canyon 252 Inc ident" (NOAA Safety D ocum entation Requlrem ents.doc).

• Exception: If a specific team 's site co llection activ ities do no t Include use o f e ithe r a boat or an a irc ra ft, then fa m ilia r ity w ith th e safety docum ents fo r these vehicles Is no t required.

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• A t least one w eek prior to th e s ta rt o f fie ld sampling, the pro ject leader w ill In form the NOAA NRDA Field Ops o f operations. This email should ou tline specific In fo rm ation regarding th e opera tion , Including at a m in im um :

o Point o f Contact, Including cell phone num ber and email,o Num ber o f fie ld team s and num ber o f samplers per fie ld team . Including contact

In fo rm ation fo r federa l representatives, o S tart date,o Num ber o f antic ipated fie ld days,o W he the r assistance in securing vessels w ill be needed,o General descrip tion o f p lanned activities,o General operationa l area(s), ando W he the r sam ple/data Intake w ill be needed.

• Before conducting fie ldw o rk , the fo llo w in g w ill be com pleted:

• Team Tracking Field Plan - Updated dally to provide Field Ops w ith In fo rm a tion about your opera tions (staff, operationa l area, launch, contact In form ation)

• Site Safety Plan - The team w ill be expected to review and com ply w ith th e cu rren t Site Safety Plan accessed on noaanrda.org In th e D ocum entation: Safety Protocols section. The pro ject leader m ust de te rm ine w h a t requ ired safety equ ipm ent th e team needs

• Field teams m ust adhere to all procedures set fo r th In th e "D eepw ate r Florlzon NRDA Site Safety Plan Version 12 /08 /2010 ," ("NRDA_OPS_Safety_Plan_08DEC2010.docx").®

IV. Site Selection Strategy

Sampling Is Intended to encompass an area w here a grad ien t from heavy to lit t le o r no o iling Is an tic ipa ted to exist. Sampling w ill be conducted In w aters betw een 0 m to 10 m In depth from approx im ate ly 20 km w est o f Terrebonne Bay around Pass a Loutre to a po in t on th e northeastern coast o f th e Mississippi River Delta (see Figure 2). It Is an tic ipated th a t the o iling level In Baratarla Bay may be on th e high end o f the gradient, as th is area Is still active ly being cleaned up, and th a t th e o iling level may d im in ish m oving w estw ard.

Sampling w ith in th is area w ill be subdivided Into 5 strata: (I) Baratarla Bay Inshore,(II) Baratarla Bay o ffshore, (III) Pass a Loutre, (IV) T e rrebonne /T lm ba lle r Bay Inshore, and (V) Terrebonne /T im ba lle r Bay o ffshore (see Figure 1). The locations o f sam pling stations w ith in each strata w ere a llocated using a generalized tesse lla tion s tra tified sam pling (GRTS) scheme (Stevens and Olsen 1999; Stevens and Olsen2004). For planning purposes, a GRTS sample o f 30 locations was selected In each s tra tum to receive fie ld m easurem ents. A lte rna te locations w ere also created In th e GRTS procedure to be used In the event th a t one or m ore o f these 30 locations are de term ined, at th e d iscretion o f fie ld sam pling team s, to be unsuitable fo r sampling. Sites th a t are unsuitable fo r sam pling by one o r m ore gear types w ill no t be visited, bu t w ill be replaced by the next site selected In th e GRTS procedure and th e reason fo r any

This file Is available orj

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changes w ill be noted on data co llectlon sheets. This w ill ensure th a t gear can be deployed successfully at all sites.

Marine Mammal Prey Sampling: All Strata strata ii. iii, & v e x te n dto 10m bathymetric contour

10 20 30 40]K m

Data Source N R D A M ai me Mammal Tnisitee W oikm s Gioup ^ Proiection Umveral Traiisvei^^e Mercator (U T M iWEST Coordinate Reference System: NAD1983 l^T M zone 15N

Cieatedbv Giant Gaulnei Date l0 M a jc li2 0 1 1

Legend 1:845,066

o G R T S sam p le locations

S tra tu m I - B aratarla B ay inshore

S tra tu m I I - B aratarla B ay offshore

S tra tu m III - P ass a Loutre

S tra tu m IV - T erre b o n n e /T im b a lle r B ay inshore

S tra tu m V - T erre b o n n e /T im b a lie r B ay o ffshore

Figure 1: Sampling stations, w ith in each stra tum , th a t w ere a llocated using a generalized tessella tion s tra tified sampling (GRTS) scheme (Stevens and Olsen 1999; Stevens and Olsen 2004).

V. Site procedures

Targeted p rey Item sam pling

The goal o f th e sam pling e ffo rt under th is Plan is to obta in w ho le samples and muscle tissue from nearshore cetacean, Kemp's rid ley and loggerhead sea tu r t le prey item s th a t w ill be archived per NRDA sam ple storage pro toco l (see Appendix 1) fo r contam inan t analysis at a fu tu re date. A to ta l o f 150 sam pling stations have been allocated over all 5 strata (5 strata conta in ing 30 stations each). This is in tended to ensure th a t an adequate num ber o f sites are sampled in each stra tum . Individual stations w ill be sampled once every 5 m onths fo r a to ta l o f 10 m onths (beginning upon signature o f th is plan), th e stations w ith in each o f th e 5 stra ta being sampled as a set. Field team s w ill thus ro ta te from one

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stra tum to th e next in sequence, on a m on th ly basis, so th a t specimens from each s tra tum w ill be sam pled during d iffe re n t seasons over th e course o f th e study. It is an tic ipated th a t strata w ill be sam pled in th e fo llo w in g o rde r (see Figure 2): Barataria Bay inshore (Stratum I), Barataria Bay offshore (S tratum II), Pass a Loutre (Stratum III), T errebonne /T im ba lie r Bay inshore (S tratum IV), T errebonne /T im ba lie r Bay o ffshore (S tratum V). Such sequencing may change based upon tim e o f in itia tio n , seasonal considerations, o r p re lim inary results. Sampling w ill be adjusted to avoid sampling in th e seasonal (late sum m er) hypoxic zone. This zone is n o t fixed from season to season, bu t is located p rim a rily in S tratum II.

The Trustees and BP w ill m eet (e ithe r in person o r by phone) a fte r seven m onths o f sampling have been com ple ted to discuss th e need fo r an add itiona l round o f sampling. If BP decides it w ill no longer fund th e study a fte r 10 m onths, th e Trustees may proceed independently. BP may also cease fund ing and w ith d ra w from th e study if 45 days pass w ith o u t such a m eeting despite BP's good fa ith e ffo rts to schedule one. In e ithe r case, should th e Trustees continue the study, BP agrees n o t to in itia te fie ld w ork th a t duplicates o r overlaps w ith the Trustees' prey sam pling e ffo rts .

Field team s w ill use gill nets and traw ls in in itia l sampling effo rts . W here sampling stations are located at depths less than 8 fee t, and w ith cond itions appropria te fo r th e use o f gill nets (to be de term ined at th e d iscretion o f fie ld sampling team s), fie ld team s w ill u tilize (strike) gill nets once and then tra w l the study site once. Trawls w ill be tow ed fo r 10 m inutes each (per LDWF 2010). Procedures fo r decon tam ina tion o f these gears w ill be included in th e SOPs in Appendix I. Effectiveness o f these gears w ill be evaluated a fte r each sam pling event and fie ld protoco ls w ill be am ended if de term ined necessary by th e Trustees and BP. Catch w ill be sorted and iden tified to species.

Any w ho le body fish < 600 mm in length w ill be preserved separately fo r analysis by w rapping in p recleaned a lum inum fo il (provided by Newfie lds lab) and s toring on ice un til it can be frozen at -20°C (w ith in 24 hours o f sample co llection). For fish > 600 mm in length, muscle tissue samples (one sample) w ill be taken as described above in section II, and preserved separately. For species < 30 g, stored samples w ill consist o f groups o f one species taken from one site using one type o f gear. For example, all w h ite shrim p from one site caught w ith traw ls w ill be w rapped in fo il toge the r. All samples w ill be co llected by data intake team s w ho w ill m ee t fie ld sampling team s at the dock at th e end o f each sam pling day.

VI. Data Handling and Sharing

A. D ata Handling

MG 252 NRDA chain-of-custody procedures w ill be observed fo r all samples. All samples w ill be trans fe rred w ith appropria te cha in-of-custody form s. Camera m em ory cards w ill be handled under Chain-of-Custody a fte r a card is fu ll o r a fte r th e study is com pleted pursuant to th e NRDA Field Sampler Data M anagem ent Protocol, 10-24-2010, w hich includes th e pro toco l fo r trans fe rring and uploading d ig ita l photos (m ost up-to -da te versions o f all NRDA protoco ls available at noaanrda.org).

All fie ld and labora to ry data w ill be collected, managed and stored in a secure fa c ility under trus tee con tro l in accordance w ith w ritte n SOPs. The appropria te tra in ing on particu la r equ ipm ent o r in the conduct o f specific fie ld studies fo r all personnel involved w ith th e p ro ject shall be docum ented and those records shall be kept on file fo r the du ra tion o f th is pro ject.

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All m ateria ls associated w ith th e co llectlon o r analysis o f samples under these protoco ls o r pursuant to any approved w ork plan, except those consum ed as a consequence o f th e applicable sampling or analytical process, must be re ta ined unless and un til approval Is given fo r th e ir disposal In accordance w ith the re ten tion requ irem ents set fo r th In paragraph 14 o f Pretrial O rder # 1 (Issued August 10, 2010) and any o the r applicable C ourt Orders governing tang ib le Items th a t are or may be Issued In MDL No. 2179 IN RE: Oil Spill by th e Oil Rig "DEEPWATER HORIZON" (E.D. LA 2010). Such approval to dispose m ust be given In w rit in g and by a person authorized to d irect such action on beha lf o f th e state or federa l agency whose employees o r contractors are In possession or con tro l o f such m aterials.

B. D ata Sharing

Copies o f all non-analytlca l data collected In accordance w ith th is plan, (Including raw data, fie ld sheets, and fie ld notes, photos, photo logger fo rm s and GPS files), w ill be transfe rred to th e NOAA NRDA Sample Intake Team fo llow ing NRDA data m anagem ent protocols. An Identical copy o f all docum enta tion w ill be provided to BP/Cardno-ENTRIX and th e Louisiana Oil Spill C oord ina tor's Office (LOSCO) w ith in a reasonable tim e fram e once data Intake, QA analyses and data e n try procedures are com ple te bu t no la te r than 45 days a fte r th e data are collected. All samples collected pursuant to th is Plan w ill be subm itted to laboratories th a t are operated In a m anner th a t is consistent w ith the guidelines o f th e Analytical Q ua lity Assurance Plan (A Q A P )fo rth e M ississippi Canyon (D eepwater Horizon) Natural Resource Damage Assessment (version 2.2).

This Plan does no t include any labo ra to ry analysis. The fo llo w in g provision w ill apply to any labora to ry data generated pursuant to a cooperative addendum developed as described above In Section I:

Each labora to ry shall s im ultaneously de liver raw data. Including all necessary m etadata, generated as pa rt o f th is w o rk plan as a Laboratory Analytical Data Package (LADP) to th e trus tee Data M anagem ent Team (DMT), th e Louisiana Oil Spill C oord inator's O ffice (LOSCO) on behalf o f the State o f Louisiana and to BP (o r ENTRIX on behalf o f BP). The e lectron ic data deliverab le (EDD) spreadsheet w ith pre-valldated analytical results, w hich Is a com ponent o f th e com ple te LADP, w ill also be delivered to the secure FTP d rop box m ainta ined by the trustees ' Data M anagem ent Team (DMT). Any p re lim ina ry data d is tribu ted to th e DMT shall also be d is tribu ted to LOSCO and to BP (or ENTRIX on beha lf o f BP). Thereafter, the DMT w ill va lidate and perfo rm qua lity assurance/quality con tro l (QA/QC) procedures on th e LADP consistent w ith the authorized Analytical Q ua lity Assurance Plan, a fte r w hich tim e th e va lidated/Q A /Q C data shall be made available s im ultaneously to all trustees and BP (or ENTRIX on beha lf o f BP). Any questions raised on the va lidated/Q A /Q C results shall be handled per the procedures In th e Analytical Q ua lity Assurance Plan and th e Issue and results shall be d is tribu ted to all parties. In th e Interest o f m a in ta in ing one consistent data set fo r use by all parties, on ly th e va lida ted /Q A /Q C data set released by th e DMT shall be considered th e consensus data set. In o rde r to ensure re liab ility o f th e consensus data and fu ll review by th e parties, no party shall publish consensus data un til 7 days a fte r such data has been made available to th e parties. Also, th e LADP shall no t be released by the DMT, LOSCO, BP or ENTRIX p rio r to va llda tlon /Q A /Q C , absent a show ing o f critica l operationa l need. Should any party show a critica l operationa l need fo r data p rio r to va lidatlon/Q A /Q C , any released data w ill be clearly marked "p re llm lna ry /unva lida ted " and w ill be made available equally to all trustees and to BP (or ENTRIX on beha lf o f BP).

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Lead Investigators

Description of Duties for Lead InvestigatorsName RoleM andyTum linLouisiana D epartm ent o f W ild life and Fisheries

Principal Investigator

ReferencesBarros, N. B. and D. K. Odell. 1990. Food habits o f bottlenose dolphins in the southeastern

United States. In. Eds. S. Leatherwood and R. R. Reeves. The bottlenose dolphin. Academic Press, San Diego, California.

Barros, N.B., & Wells, R.S.1998. Prey and feeding patterns o f resident bottlenose dolphin (Turslops truncatus) in Sarasota Bay, Florida. Journal o f M am m alogy, 79(3): 1045-1059.

Bjorndal, K. (1996). "Foraging Ecology and N utrition o f Sea Turtles." In Lutz, P.L., Musick, J.A., Wyneken, J. (Eds.) The Biology o f Sea Turtles (pp. 199 - 232). Boca Raton, EL: CRC Press.

Louisiana Departm ent o f W ild life and Fisheries, (2010).Office Of Fisheries, "Deepwater Horizon(Mc252) Oil Spill Impact: Inshore Fisheries M onitoring Plan", September 1, 2010

Maze-Foley, K. M ullln, K.D. 2006. Cetaceans o f the oceanic northern Gulf o f Mexico:D istributions, group sizes and interspecific associations. J. Cetacean Res. Manage. 8(2)L 203-213.

National Marine Fisheries Service, 2010. 2009 Report to Congress on the Status o f U.S. Fisheries. This and archive o f Annual Status o f U.S. Fisheries Reports available at: h ttp ://www.nm fs.noaa.gov/sfa/statusoffisheries/SQ Sm ain.htm

National Marine Fisheries Service and U.S. Fish and W ild life Service. 2008. Recovery Plan fo r th e Northwest Atlantic Population o f the Loggerhead Sea Turtle (Caretta caretta). Second Revision. National Marine Fisheries Service, Silver Spring, MD.

National Marine Fisheries Service and U.S. Fish and W ild life Service. 1993. Recovery Plan fo r Hawksbill Turtles in the U.S. Caribbean Sea, A tlantic Ocean, and Gulf o f Mexico. National Marine Fisheries Service, St. Petersburg, Florida.

National Marine Fisheries Service and U.S. Fish and W ild life Service. 1992. Recovery Plan fo r th e Kemp's Ridley Sea Turtle (Lepidocheivs kempii). National Marine Fisheries Service, St. Petersburg, Florida.

National Marine Fisheries Service and U.S. Fish and W ild life Service. 1992. Recovery plan fo r leatherback tu rtles (Dermochelys coriacea) in the U.S. Caribbean, A tlantic, and Gulf o f Mexico. National Marine Fisheries Service, W ashington, D.C. 65pp.

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http://www.nmfs.noaa.gov/sfa/statusoffisheries/SQSmain.htm


National Marine Fisheries Service and U.S. Fish and W ild life Service. 1991. Recovery Plan fo r U.S. Population o f A tlantic Green Turtle. National Marine Fisheries Service, Washington,D.C.

Peterson, C.H., Rice, S.D., Short, J.W., Esier, D., Bodkin, J.L, Ballachey, B.E., Irons, D.B. 2003. Long-term ecosystem response to the Exxon Valdez oil spill. Science, 302 (5653), 2082- 2086.

Stevens, J., Don L., and A. R. Olsen. 1999. Spatially Restricted Surveys Over Time fo r Aquatic Resources. Journal o f Agricultural, Biological, and Environmental Statistics 4:415-428.

Stevens, J., Don L., and A. R. Olsen. 2004. Spatially Balanced Sampling o f Natural Resources. Journal o f the American Statistica l Association 99:262-278.

W aring GT, Josephson E, Maze-Foley K, and Rosel PE, editors. 2009. U.S. A tlan tic and G ulf o f Mexico M arine M am m al Stock Assessments — 2009. NOAATech Memo NMFS NE 213; 528p.

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2 0 1 1 BUDGET ESTIMATE

This budget assumes that only one stratum will be sampled at a tim e. Sampling at more than one location concurrently w ill increase costs; this budget does not include analytical costs.

DESCRIPTION AM OUNT

INITIAL ONE-TIME EXPENDITURES;

2 CHEST FREEZERS (-20°C) $ 3,000

SAMPLING GEAR AND ACCESSORIES INCLUDING:GILL NETS, TRAWLS, WEIGHTS, FLOATS, ROPES, ETC. $ 26,265

STAFF/SALARY

6 LDWF STAFF MEMBERS AT A RATE OF 40 HOURS PER WEEK @ $22.99/HR (INCLUDES SAMPLING, PREP TIME, DATA ENTRY AND COMPILATION, SAMPLE STORAGE, CLEANING, ETC.)

$ 220,704

FRINGE BENEFITS (34%) $75 ,040

VESSEL OPERATING EXPENDITURES

FOR VESSELS UTILIZED DURING SAMPLING REGIME $ 91,960

VEHICLE USEAGE $ 7,500

TRAVEL

LODGING (WHERE DEPARTMENT FACILITIES ARE NOT AVAILABLE) $ 4,000

SAMPLING SUPPLIES

MISCELLANEOUS SUPPLIES $ 30,000

TOTAL FOR 10 MONTHS $458,469

The Parties acknowledge that this budget is an estimate and that actual costs may prove to be higher.BP's com m itm ent to fund the costs of this w ork includes any additional reasonable costs within thescope of this approved work plan that may arise. The trustees will make a good faith effort to notify BP in advance of any such increased costs.

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Appendix 1: Fish Collection SOP

M a te ria ls

1. Small cooler w ith Ice (to hold samples un til frozen)2. Datasheets or notebooks3. Scalpel handles (#3) and blades (#11)4. Stainless steel forceps (small and large), scissors, hem ostats5. Stainless steel o r Teflon cu tting board6. Pre-cleaned a lum inum fo il7. N Itrlle o r o th e r non-contam lnating gloves8. Isopropanol o r 95% ethanol In a squ irt b o ttle9. D istilled w a te r10. A lconox soap fo r cleaning equ ipm ent and too ls11. Clear vial and spring Inserts fo r low -vo lum e com posite samples (h ttp ://w w w .su n -s ri.co m ;

catalog num bers 200-774 and 200-778, respectively).12. 20-mL sc in tilla tion vials fo r use w ith large fishes13. 1-cc tube rcu lin syringes w ith needles14. Marking pens with Indelible ink for labeling tasks15. Chain o f Custody Forms and security tape16. Clear packing tape17. Sharps conta iners (fo r scalpel blade disposal) and garbage bags.

Sample Labeling and Packaging Guidelines

1. All parts o f th e anim al m ust be reta ined.2. A single fish or a sample o f a g roup o f fish o f th e same species w ill be assigned a single NRDA ID

num ber.3. Each com ponent (carcass an d /o r tissue) w ill be labeled as "A " o r "B" (e.g., LAAM24-A0502-

T0102A, LAAM24-A0502-T0102B fo r th e tissue and carcass).4. Carcass and tissue samples w ill each be w rapped separate ly In pre-cleaned a lum inum fo il,

placed In separate plastic bags w ith the NRDA ID num ber w ritte n on the bag and on a clo th tag conta ined w ith in th e bag.

5. Each sample (com posite, tissue, etc.) should have Its ow n bag w ith accom panying sample name and tag, as specified. Subsamples fro m a single fish can be bagged to g e th e r fo r convenience, provided th a t all NRDA sample IDs and pe rtinen t In fo rm ation are w ritte n on the bag and also on a c lo th tag w ith in th e bag.

6. All samples should be Iced as soon as practicable, and archived at -20 °C fo r pending analyses.

Sample Processing Guidelines

1. Place fish into a covered storage container (polypropylene or stainless steel)tem porarily , until each can be frozen or subsampled (samples w ill spend as little tim e as possible sitting ou t In the open air on the deck o f the ship).

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http://www.sun-sri.com


2. A subsample o f muscle tissue should be taken from fish >600 mm in length (see 4-7 below)

3. Fish samples should be wrapped in pre-cleaned alum inum fo il (provided by Newfields lab), placed in plastic freezer bag, labeled per NRDA sample collection protocol, and placed in freezer at -4°C or on ice fo r tem porary storage until sample can be stored at - 20°C pending analysis.

4. Clean table w ith Alconox solution and seawater5. Put on a clean pair o f n itrile gloves6. Obtain a new disposable scalpel blade: muscle tissue sampler should change scalpel

blades between subsamples o f each fish.7. Once fish is on the table, carefully clean the external area to be subsampled w ith dihl20.

a. A fte r sacrificing each animal (by severing the spinal column just posterior to the fish's head), open the body cavity using scissors, forceps or new disposable scalpel blade. One set o f tools w ill be used to cut open the animal and a separate set o f tools w ill be used fo r cutting tissue sections inside the animal.

b. Take approxim ately 30 g o r more o f muscle tissue from behind the head.8. W rap and label each sample independently in pre-cleaned alum inum fo il imm ediately

a fte r sample is collected.9. Place alum inum fo il-w rapped tissue in plastic freezer bag; let as much air as possible out

o f bag and seal bag, label per NRDA sample collection protocol.10. Place in freezer at -4°C or on ice fo r tem porary storage.

Recording protocols

The fo llow ing inform ation should be recorded on the sampling sheets:1. Collection date2. Station number3. Species and gender (if possible, per NMFS standard protocol)4. Sequential specimen # (assigned by person collecting sample; start over at each station)5. Any o the r in form ation required by NRDA sample collection instructions6. Length and weight o f specimen

Equipment cleaning procedures1. Rinse dissecting tools w ith isopropyl between each fish.2. If tools are d irty w ith caked-on tissue, wash in Alconox solution, rinse w ith diFl20, and

then rinse w ith isopropyl alcohol.3. Don't wash scalpel blades; just replace w ith a new scalpel blade.

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Appendix 2: Protected Species Mitigation Measures

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for the marine mammals and sea turtles working group of ...jul 06, 2011 · finley^ kym walsh^...

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