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NEW Fluorescence Imaging Interference Filters

Introducing NEW multiband filters for fluorescence detection Available individually or as sets. Each set has 3 multi bandpass filters (excitation, emission, dichroic), and the “Pinkel” versions of these three new sets contain separate narrow band excitation filters for selected excitation of each fluorophore.

Multi band pass filters are widely used in fluorescence imaging applications as a means of rapidly identifying positively labeled multi color samples. The filters are optimized to provide high transmission, steep edges and deep blocking for balance in contrast, brightness, and color representation.

All filters are manufactured using Omega Optical’s proprietary MEGALux © optical coating technology.

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Fluorophores Filter Set # Notes Components

Type Part # Description

FITC/ TRITC or eGFP/ DsRed2

XF452 High throughput filter set for green and orange emitting fluorophores such as FITC and TRITC. This set offers excellent contrast and can also be used with Alexa Fluor® 488, Cy2, and GFP-like fluorescent proteins, as well as Alexa Fluor®568 and tdTomato.

Exciter XF1450 490-550DBEX

Dichroic XF2443 490-550DBDR

Emitter XF3456 520-580DBEM

FITC/Texas Red® or eGFP/mCherry

XF453 XF453 is optimized for use with the fluorescent proteins eGFP and mCherry. This high contrast set utilizes the 577nm Mercury peak for efficient excitation of red emitting fluorophores and is also compatible with many other common fluors such as FITC and Texas Red®.

Exciter XF1451 484-575DBEX

Dichroic XF2044 490-575DBDR

Emitter XF3457 525-637DBEM

FITC/ Cy5® XF454 Due to their wavelength separation, FITC and Cy5 make a popular choice for dual labeling in a single sample as spectral bleedthrough is virtually non-existent. This set is ideal for any green and far red emitting fluors. Other compatible dyes are, Alexa Fluor®488, Hylite 488, Oregon Green, Cy2, and Alexa Fluor®647, Hylite 647.

Exciter XF1409 475-625DBEX

Dichroic XF2401 475-625DBDR

Emitter XF3404 535-710DBEM

FITC/TRITC or eGFP/DsRed2

XF452-1 Pinkel – a set contains 2 bandpass excitation filters, single multiband dichroic beamsplitter and single multiband emission filter.

Exciter #1 XF1404 480QV20

Exciter #2 XF1405 555QV25

Dichroic XF2443 490-550DBDR

Emitter XF3456 520-580DBEM

FITC/Texas Red® or eGFP/mCherry

XF453-1 Pinkel – a set contains 2 bandpass excitation filters, single multiband dichroic beamsplitter and single multiband emission filter.

Exciter #1 XF1406 490QV20

Exciter #2 XF1407 575QV30

Dichroic XF2044 490-575DBDR

Emitter XF3457 525-637DBEM

FITC/ Cy5® XF454-1 Pinkel – a set contains 2 bandpass excitation filters, single multiband dichroic beamsplitter and single multiband emission filter.

Exciter #1 XF1405 480QV20

Exciter #2 XF1410 630QV40

Dichroic XF2401 475-625DBDR

Emitter XF3404 535-710DBEM

Dual Band Filters

Excitation and Emission Filters: 12.5, 18, and 25 mm round

Dichroic Beamsplitters: 18x26, 25.7x36 mm rectangular, and 25 mm round

Custom sizes up to 55 mm available. Comparable pricing to catalog list prices. Call for details.

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PINkEl FIlTER SETS

Obtaining a high quality image using a black and white CCD camera requires a different approach. Pinkel filter sets selectively excite one fluorophore in a multi labeled sample through the use of fluor specific narrow band excitation filters housed in a filter wheel or slider. These filters are used with a multi band dichroic mirror and emission filter which remain in the filter holder turret. This design reduces the spectral bleed-through of the traditional multiband filter set, and minimizes sample movement by isolating the wheel from the microscope stage.

XF467 Triple stained images of mouse brain (page 1) and Muntjac deer cells (page 2).

Courtesy of Mike Davidson at the National High Magnetic Lab, Florida State University.

Fluorophores Filter Set # Notes Components

Type Part # Description

DAPI/FITC/Texas Red® or BFP/eGFP/mCherry

XF467 This filter set is optimized for use with common blue, green, red emitting fluors such as DAPI/ FITC/Texas Red® or proteins BFP/eGFP/mCherry. The set can be used with visual detection, a CCD camera or color film for image capture.

Exciter XF1458 390-486-577TBEX

Dichroic XF2045 400-485-580TBDR

Emitter XF3458 457-528-600TBEM

DAPI/FITC/Texas Red® or BFP/eGFP/mCherry

XF467-1 Pinkel Exciter #1 XF1408 405QV20Exciter #2 XF1406 490QV20Exciter #3 XF1407 575QV30Dichroic XF2045 400-485-580TBDREmitter XF3458 457-528-600TBEM

triple Band Filters

XF467-1 Triple Band Set for DAPI/FITC/ Texas Red®

For more information on all stock filters

along with an expanded list of compatible dyes,

please visit our interactive tool Curvomatic at www.omegafilters.com

Excitation and Emission Filters: 12.5, 18, and 25 mm round

Dichroic Beamsplitters: 18x26, 25.7x36 mm rectangular, and 25 mm round

Custom sizes up to 55 mm available. Comparable pricing to catalog list prices. Call for details.

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