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Fig. 1: long-term protocoll. Fig. 2a: short-term protocol. Fig. 2b: short-term protocol. Figure 1: Immunoglobulin titers in OVA-sensitized BN rats : long-term protocol - PowerPoint PPT Presentation

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Page 1: Fig. 1: long-term protocoll

Fig. 1: long-term protocoll

Page 2: Fig. 1: long-term protocoll

Fig. 2a: short-term protocol

Page 3: Fig. 1: long-term protocoll

Fig. 2b: short-term protocol

Page 4: Fig. 1: long-term protocoll

Figure 1: Immunoglobulin titers in OVA-sensitized BN rats: long-term protocolAnimals were sensitized by intraperitoneal injection of OVA (10 µg, 100 µg, or 1000 µg) once every second week for a total of 12 weeks (long-term protocol). The allergen of the first injection was absorbed to 2 mg aluminum hydroxide (AL) and B. pertussis whole body vaccine (PV) (2x106 PV, E. von Behring, Marburg, Germany). Blood samples were taken at indicated time points. Serum levels of allergen-specific IgE and total IgE were measured by ELISA.

Figure 2 a and b: Immunoglobulin titers in OVA-sensitized BN rats: short-term protocolAnimals were sensitized by intraperitoneal injection of 10 µg OVA on days 1, 5 and 10 (short-term protocol). To assess the effect of adjuvants on sensitization, the allergen of the first injection was absorbed to 2 mg aluminum hydroxide (AL) and/or B. pertussis whole body vaccine (PV) (2x106 PV, E. von Behring, Marburg, Germany). Blood samples were taken at day 24. Serum levels of total IgE (figure 2a) and allergen-specific IgE (figure 2b) were measured by ELISA.

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Fig. 3

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Figure 3: Intestinal changes upon local allergen challenge

BN were sensitized by intraperitoneal injection of 10 µg OVA on days 1, 5 and 10 (short-term protocol). Allergen was absorbed to aluminium hydroxide (2 mg AL) and pertussis whole body vaccine (2x106 PV, E. von Behring, Marburg, Germany) at the first injection timepoint. Sensitized animals according to the short-term protocol were challenged locally by gavage feeding of 500 µg OVA (in 1 ml PBS) twice, on days 20 and 21 (two challenges) (3a), or on ten consecutive days, beginning on day 20 (repeated challenges) (3c). The negative control groups were sham-sensitized and challenged with PBS according to the same protocols (3b). All animals were sacrificed and analyzed 24 h after the last allergen challenge. Intestinal tissue was fixed in 4% formalin, dehydrated, mounted in paraffin, sectioned, and stained with hematoxylin-eosin (HE) or periodic acid-Schiff (PAS) reaction by using a standard protocol.


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