field evaluation of host plant resistance in …...field evaluation of host plant resistance in...
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Field Evaluation of Host Plant Resistance in Strawberry Against Verticillium Wilt, Macrophomina
Crown Rot and Fusarium Wilt
G.J. Holmes, S. M. Mansouripour, J. Winslow, B. Gomez-Soto, B. Liu and T. Gordon
Orlando, FL
Pathology Automation
Entomology
NEXT-GEN DISEASE RESISTANCE BREEDING
1 = highest priority; 2 = medium priority; 3 = lowest priority; N = non-priority. SD = short-day, DN = day-neutral, and NP = nursery production.
R-gene = resistance gene, QTL = large-effect quantitative trait locus, complex = polygenic, complex genetics, and ? = unknown or hypothesized.
Portola Monterey
VERTICILLIUM TRIAL 2015
San Andreas Albion
Fusarium wilt
Natural
Cal PolyField 25
Verticillium wilt
Natural
Cal PolyField 35b
Macrophominacrown rot
Artificial
Monterey Bay AcademyLocation
Disease
Inoculum
90 genotypes30 cultivars60 elite lines4 replicates
14 DAP
Reading plant mortality every two weeks
Drone imaging every two weeks after planting
VERTICILLIUM WILT SUSCEPTIBILITY
VERTICILLIUM WILT SUSCEPTIBILITY
UC PSI Plan
asa
LC Driscoll’s
0
10
20
30
40
50
60
70
80
90
100
UC-
5DR
-8DR
-20
Man
resa
UC-
6DR
-7U
C-4
UC-
9U
C-14
Peta
lum
aM
arqu
isU
C-11
PS 9
271
DR-1
3DR
-11
Amad
oDR
-3PE
3.2
11PS
I-5DR
-17
DR-1
4Fr
onte
ras
PE 3
.221
PS 3
.108
Sens
atio
nDe
l Rey
UC-
15PE
6.2
036
PE 7
.205
9PS
I-6DR
-6PL
-02-
32Bi
g Su
rDR
-10
Cabr
illo
PSI-7
DR-1
6U
C-1
PL-0
5-10
0RBG
431
6En
chan
teU
C-13
Celin
ePS
I-8LC
-5U
C-12
Beau
tyPS
I-4U
C-10
UC-
16BG
3.3
24DR
-9O
sceo
laBG
4.3
67PL
-09-
11U
C-7
UC-
3LC
-2U
C-8
PS 5
016
BG4.
352
LC-8
PL-0
8-22
RU
C-2
LC-7
DR-1
Mav
eric
kDR
-19
PSI-2
DR-1
2El
yana
DR-5
PSI-1
DR-4
DR-1
5La
redo
Swee
t Ann
Win
ters
tar
FL 1
3.26
-134
Fest
ival
DR-1
8Ra
dian
ceBG
6.3
024
DR-2
LC-9
LC-3
PSI-3
LC-4
Ruby
June
Mon
tere
yLC
-6
Mor
talit
y (%
)
Cultivar/coded genotype
Verticillium Wilt - 2018
Marquis
Osceola
Festival
Verticillium Field
Week43
Week30
VERTICILLIUM TRIALJuly 15, 2017
Monterey (Vert-S)
0
10
20
30
40
50
60
70
80
90
100
30-Oct 30-Nov 31-Dec 31-Jan 28-Feb 31-Mar 30-Apr 31-May 30-Jun 31-Jul
Gre
en co
vera
ge(%
)
Date
Mor
talit
y (%
)
`
Manresa (Vert-R)
0
10
20
30
40
50
60
70
80
90
100
0
10
20
30
40
50
60
70
80
90
100
30-Oct 30-Nov 31-Dec 31-Jan 28-Feb 31-Mar 30-Apr 31-May 30-Jun 31-Jul
Gre
en co
vera
ge(%
)
Gre
en co
vera
ge(%
)
Date
=Manresa-R=Monterey-S
`
0
10
20
30
40
50
60
70
80
90
100
Mor
talit
y (%
)
Cultivar name
Verticillium wilt
2017 AVG = 30.12018 AVG = 11.6
∆ = 61.5%
March 1, 2017
MACROPHOMINA TRIAL
14 DAP
MACROPHOMINA CROWN ROTSUSCEPTIBILITY
MACROPHOMINA CROWN ROTSUSCEPTIBILITY
UCPSI Pl
anas
a
LC Driscoll’s
August 1, 2017MACROPHOMINA TRIAL
Festival
Fronteras
Manresa
0
10
20
30
40
50
60
70
80
90
100
DR-R
Lare
doU
C-K
PS 9
271
Osc
eola
UC-
MDR
-EDR
-KDR
-NDR
-HM
anre
saDR
-LU
C-L
DR-T
DR-D
DR-S
DR-J
Amad
oBG
6.3
024
UC-
iDR
-GPS
I-CG
rena
daPE
6.2
036
BG 4
316
PSI-D
DR-O
Big
Sur
Ench
ante
Sens
atio
nDR
-iBG
4.3
67M
arqu
isU
C-E
UC-
OU
C-N
DR-M
LC-B
Fron
tera
sCe
line
Mav
eric
kPE
7.2
059
UC-
JPL
-09-
11PS
I-HBG
4.3
52De
l Rey
DR-Q
DR-F
BG 3
.324
PSI-E
PSI-F
UC-
APE
3.2
11PS
I-BPe
talu
ma
PSI-G
PS 5
016
PL-0
2-32
PS 3
.108
DR-C
UC-
DDR
-PLC
-iU
C-F
Mon
tere
yLC
-GPE
3.2
21FL
13.
26-1
34Be
auty
Albi
onRa
dian
cePS
I-ALC
-HU
C-B
DR-A
UC-
CPL
-08-
22R
LC-E
Cabr
illo
Fest
ival
LC-C
UC-
GU
C-H
PL-0
5-10
0RSw
eet A
nnDR
-BRu
by Ju
neLC
-FLC
-D
Mor
talit
y(%
)
Cultivar/coded genotype
Macrophomina Crown Rot - 2018
OsceolaDel Rey
Ruby June
Verticillium Field Macrophomina Field
Week43
Week30
Ruby June (Mac-S)
0
10
20
30
40
50
60
70
80
90
100
30-Oct 30-Nov 31-Dec 31-Jan 28-Feb 31-Mar 30-Apr 31-May 30-Jun 31-Jul
Gre
en C
over
age
(%)
Date
Mor
talit
y (%
)
`
Osceola (Mac-R)
0
10
20
30
40
50
60
70
80
90
100
0
10
20
30
40
50
60
70
80
90
100
30-Oct 30-Nov 31-Dec 31-Jan 28-Feb 31-Mar 30-Apr 31-May 30-Jun 31-Jul
Gre
en C
over
age
(%)
Gre
en C
over
age
(%)
Date
=Ruby June-S=Osceola-R
`
0
10
20
30
40
50
60
70
80
90
100
Mor
talit
y (%
)
Cultivar name
Macrophomina crown rot
2018 AVG= 24.02017 AVG= 46.8
∆ = 48.7%
50
60
70
80
90
100
110
1-O
ct8-
Oct
15-O
ct22
-Oct
29-O
ct5-
Nov
12-N
ov19
-Nov
26-N
ov3-
Dec
10-D
ec17
-Dec
24-D
ec31
-Dec
7-Ja
n14
-Jan
21-J
an28
-Jan
4-Fe
b11
-Feb
18-F
eb25
-Feb
4-M
ar11
-Mar
18-M
ar25
-Mar
1-Ap
r8-
Apr
15-A
pr22
-Apr
29-A
pr6-
May
13-M
ay20
-May
27-M
ay3-
Jun
10-J
un17
-Jun
24-J
un1-
Jul
8-Ju
l15
-Jul
22-J
ul29
-Jul
5-Au
g
Tem
pera
ture
(F)
Date
Daily Max TemperaturePlantingTermination
Heat Wave
Water stress
50
60
70
80
90
100
110
1-O
ct8-
Oct
15-O
ct22
-Oct
29-O
ct5-
Nov
12-N
ov19
-Nov
26-N
ov3-
Dec
10-D
ec17
-Dec
24-D
ec31
-Dec
7-Ja
n14
-Jan
21-J
an28
-Jan
4-Fe
b11
-Feb
18-F
eb25
-Feb
4-M
ar11
-Mar
18-M
ar25
-Mar
1-Ap
r8-
Apr
15-A
pr22
-Apr
29-A
pr6-
May
13-M
ay20
-May
27-M
ay3-
Jun
10-J
un17
-Jun
24-J
un1-
Jul
8-Ju
l15
-Jul
22-J
ul29
-Jul
5-Au
g
Tem
pera
ture
(F)
Date
20172018
Fusarium oxysporum f.sp. fragariae
Monterey Bay Academy
FUSARIUM TRIAL 2015
FUSARIUM TRIAL 2015
9.9
16.4
26.7
33.3
32.3
55.2
53.2
75.7
96.5
0.00 20.00 40.00 60.00 80.00 100.00
Sweet Ann
Monterey
Radiance
Albion
Sabrina
Grenada
Sensation
Winterstar
Ventana
San Andreas 4.1Festival 2.3Portola 1.7
Safari 0.9Fronteras 0.6Petaluma 0.3
Percent Mortality on July 23, 2015
FUSARIUM TRIAL 2015
9.9
16.4
26.7
33.3
32.3
55.2
53.2
75.7
96.5
0.00 20.00 40.00 60.00 80.00 100.00
Sweet Ann
Monterey
Radiance
Albion
Sabrina
Grenada
Sensation
Winterstar
Ventana
San Andreas 4.1Festival 2.3Portola 1.7
Safari 0.9Fronteras 0.6Petaluma 0.3
Percent Mortality on July 23, 2015
Monterey: 75% mortality
FUSARIUM TRIAL 2015
9.9
16.4
26.7
33.3
32.3
55.2
53.2
75.7
96.5
0.00 20.00 40.00 60.00 80.00 100.00
Sweet Ann
Monterey
Radiance
Albion
Sabrina
Grenada
Sensation
Winterstar
Ventana
San Andreas 4.1Festival 2.3Portola 1.7
Safari 0.9Fronteras 0.6Petaluma 0.3
Percent Mortality on July 23, 2015
Albion: 53% mortality
FUSARIUM TRIAL 2015
9.9
16.4
26.7
33.3
32.3
55.2
53.2
75.7
96.5
0.00 20.00 40.00 60.00 80.00 100.00
Sweet Ann
Monterey
Radiance
Albion
Sabrina
Grenada
Sensation
Winterstar
Ventana
San Andreas 4.1Festival 2.3Portola 1.7
Safari 0.9Fronteras 0.6Petaluma 0.3
Percent Mortality on July 23, 2015
San Andreas: 4% mortality
FUSARIUM TRIAL 2015
9.9
16.4
26.7
33.3
32.3
55.2
53.2
75.7
96.5
0.00 20.00 40.00 60.00 80.00 100.00
Sweet Ann
Monterey
Radiance
Albion
Sabrina
Grenada
Sensation
Winterstar
Ventana
San Andreas 4.1Festival 2.3Portola 1.7
Safari 0.9Fronteras 0.6Petaluma 0.3
Percent Mortality on July 23, 2015
Portola: 2% mortality
FUSARIUM TRIAL 2015
0 1 1 2 2 410
16
2732 33
53 55
76
97
16
0 0 0 0 0
39
69
29
77
89
0
10
20
30
40
50
60
70
80
90
100
Fusarium wilt at MBA
2015 2016
% M
orta
lity
* * * *
* = not included in 2016 exp’t
CULTIVAR DISEASE RESISTANCE TABLE
Conclusions• Wide range of susceptibility among genotypes and
breeding program entries• High levels of resistance to Verticillium wilt, Fusarium
wilt and Macrophomina crown rot• Results consistent between years• Aerial images were equally effective to ground ratings
Acknowledgements
SCRI grant No.2017-51181-26833