Optical Tweezers

Fiber-light system forOlympus IX71 microscope

Fiber-light for Olympus Microscope

How to make a fiber light system for your microscope?

Optical Tweezers enclosure

Microscope wall

Microscope wall temperature inside enclosure compared: Halogen lamp Vs Fiber-light

Why fiber-light is important ?

• DNA experiments are really sensitive to temperature (thermal energy) even few degrees make a big difference.• Microscope (optical trap) is enclosed inside an enclosure (to avoid airborne noise and currents), so within few hours temperature of the microscope wall rises almost twice due to the halogen lamp used for illumination, this heats up the entire enclosure so as the sample.• Temperature hike may make two similar data sets which are taken some time apart different. • Fiber light solves the problem of temperature hike by providing clean light and by removing heat production through the lamp and conduction through the microscope skin.• With fiber light temperature inside the enclosure goes from 23.7 to 25.5 total 1.8 degree Celsius hike in comparison to halogen lamp where temperature goes from 23.7 to 49 total 25.3 degree hike over a period of 10 hours (see slide 3).

Construction of Fiber-light system

Fiber light has 4 parts

Olympus power supply Model TH4-100

Fiber-light feeder

Illuminator & fiber plugin adapter

Dolan Jenner optical fiber bundle

Olympus Power supplyModel TH4-100

TH4-100 has 4 holesConnect +ve 12V and ground with a wire at one end and other end is banana connectors

Construction of fiber plugin adapter at illuminator

Illuminator

Fiber pluginadapter

Construction Steps

Remove illuminator from microscope

Remove all the enclosuresRemove the Halogen lamp

Assemble the fiber plugin

CP02-thorlabs cage plate

ER2-thorlsba 2’’ cage assembly rod

SM1L03-thorlabs lens tube

S1TM08-thorlabs lens cell adapterAD11F-thorlabs adapter

Put lens cell adapter on the cage plate Put lens tube on different cage plate and assemble

Put other lens cell on one end of cage plate and adapter on other end

Fixing the Illuminator

Make 2 notches aligned with center of holes on the cage

center

Little stage

wall

Make a through hole ¾ cm from the notch and from the edge

Make a metal piece looks like in the picture the hole are aligned with each other

Put the piece such the hole are aligned

Put the plugin on the illuminator

Put the screws

Put a screw and a nut

Illuminator with fiber plugin adapter is complete

Cushion

Screws

Put a rubber cushion (optional) and insert the fiber through the opening and tight the screws

Z- adjustment

Construction of Fiber-feeder

Fiber outlet adapterLens assemblyLamp mount

6 X3X2.5 inches aluminum box

Prepare the box

3/8’’ holes for rubber legs

3/8’’ hole for fiber outlet adapter

¼’’ hole for lens assembly

Lamp side

Banana connectors

3/8’’ hole for lamp mount

Fiber outlet side1’’ hole for fiber outlet

2.25’’

1.25’’

.5’’

.25’’

Bottom side

50mm

.5’’

Preparing Lamp Mount

Halogen lamp

Metal plates

PCB plates

Banana connectors

4/40’’ blind hole ½’’ long in PCB

WARNING: Lamp gets really HOT so DON’T use plastic, wood or anything which burns below 500o Celsius

• I used 6 fold of PCB plates to mount the metal plates with ½’’ long blind screws.• Metal plates are aluminum plates which I machined to fit the lamp wire connectors.• Metal plates are attached into PCB plates with 4/40’’ blind screws.• PCB plates are screwed to the wall with 3/8’’ screws.• PCB plate can tolerate high temperatures and provide good isolation for the metal plates.

3/8’’ through holes

4/40’’ screw on 3/8’’ holes in metal

Preparing Fiber outlet adapter

SM1A1-Thorlabs adapterSM1T2-Thorlabs coupler

CP02T-thorlabs cage plate

Insert adapter into coupler and then coupler into cage plate

• On wood side cage plate do not use the wood like shown in the picture. • Put another cage plate on that side with another adapter and coupler.• I did not have extra set in my lab so I used wood.

Mount in with 3/8’’ screw to the box

Do not use wood use 2 cage plates with 2 couplers and 2 adapters

Preparing Lens assembly

Lens is to focus the light on the fiber inlet

CP02T-thorlabs cage plates

ER2-thorlsba 3’’ cage assembly rodTR2-thorlabs post

RA90-thorlabs post clamp

LA1951-A thorlabs f25mm lens

Put lens assembly on the box

Lens assembly gives 3-D adjustment to the lens

Slide in z-direction to get optimum spot on the fiber inlet

xy-adjustment to center the spot (lamp image) on the fiber inlet

50mm

Bad alignment

Good alignment

Spot can be further optimized by looking into illuminator

Illuminator at low intensity

Prepare the cover

• Halogen lamp heats up the box• I did not put any fan inside becausefan generates vibrations which can travel through the fiber bundle to the microscope and make optical trap noisy.• So fan has to be outside with no physical contact with the box.• To accommodate that I made 2’’diameter holes.• Fan will be mounted from top trough the roof.

Mounted Fan

Bundle is now complete

Mount the illuminator on the microscope

Fiber-light with Optical Tweezers