feedback control for the programmable cell culture chip “procell” felician Ștefan blaga...
TRANSCRIPT
Feedback Control for the Programmable Cell Culture Chip “ProCell”Felician Ștefan Blaga
Supervisor: Paul Pop (DTU Informatics)
Co-supervisors: Wajid Minhass (DTU Informatics) and Martin Dufva (DTU Nanotech)
2
OutlineEarly design phases
Design decisions and uncertaintiesModeling uncertainties
Worst-case execution time Functionality requirements
Problem formulation: mapping optimization Motivational examples
Multi-objective optimization strategy Robustness vs. flexibility Genetic Algorithm approach Experimental results
Conclusions and message
3
Motivation for Biochips
Test tubesAutomationIntegrationMiniaturization
MicrofluidicsAutomationIntegrationMiniaturization
RoboticsAutomationIntegrationMiniaturization
4
Two types of microfluidic biochips
4
Continuous-flow biochips: Permanently-etched microchannels, micropumps and microvalves, electrokinetics, etc.
Digital microfluidic biochips: Manipulation of liquids as discrete droplets
Biosensors: Optical: SPR, Fluorescence etc. Electrochemical: Amperometric, Potentiometric etc.
Mixing: Static, Diffusion Limited
Multiplexing
5
Application area: cell culturing
TODO Some text about cell culturing
6
ProCell setupRequirements:
1 2
Components 1 2
7
ProCell control diagram
Procell Prototype
Motors controlling the pumps
Signal adaptor LabJack
PC
Microscope
ExplanationsExplanations
Explanations, color change…
8
ProCell prototype
The biochip is fabricated using micro-milling prototyping
9
Zeiss MicroscopeSomething about the
microscope and the AxioVision Software
Something about fluorescence
10
Control boxSomething about the control
box, and difficulties in putting everything together (you had to order the box, etc.)
What you will cover next: LabJack Signal adapter board
11
LabJack
What is a LabJack? (copy paste from the thesis)
How is it controlled?
12
Signal adapter boardSome explanations about the
motors Use sub-bullets if needed
Something about the board and why you needed the counter
Step motor Faulhaber AM1524Ripple counter
13
Three types of control1. Direct control
Short text about it—take it from the thesis and be succinct
2. Scenario-based control Short text about it
3. Feedback control Short text about it
14
Scenario-based controlFocus on VBA
Tell us what is on the left Tell us what you did with VBA
15
Feedback controlExplain the feedback control
Tell us about VBA
Maybe we need two slides?
Main part
LabJack control Scripts control
Excel interface
Ref
-R P M
16
Evaluation: direct control
What do we see in the picture
How is the control achieved
17
Evaluation: scenario-based control
What do we see in the picture
How is the control achieved
18
Evaluation: feedback controlNo Feedback
What do we see in the picture How is the control done
0 10 20 30 40 50 60700.00
900.00
1100.00
1300.00
1500.00
1700.00 f(x) = 21.7313474074543 x + 689.395543082601R² = 0.987487473443347
No feedback
Pump percentage( %)
Imea
sure
d (a
.u.)
500 600 700 800 900 1000110012001300140015001600500.00600.00700.00800.00900.00
1000.001100.001200.001300.001400.001500.001600.00
f(x) = 1.00369053195531 x − 2.95206272115252R² = 0.999500103988698
Feedback control
Iset point(a.u.)
Imea
sure
d (a
.u.)
With feedback What do we see in the picture How is the control done
What is the conclusion
19
Evaluation: feedback controlRemind what is fluorescence
What do we see in the picture
How was the control done
Conclusion: why is it good
0.5 4
7.5 11
14.5 18
21.5 25
28.5 32
35.5 39
42.5 46
49.5 53
56.5 60
63.5 67
70.5 74
77.5 81
84.5 88
91.5
0
500
1000
1500
2000
2500
Fluorescence
Time (min)
Imea
sure
d (a
.u.)
20
Conclusions ProCell setup: cell culturing biochip, microscope, proposed control box
Several control approaches, using VBA and the control box Direct control Scenario-based control Feedback control
The control solution has been systematically evaluated and tested
The solution is successfully used in the field DTU Nanotech Danish Cancer Society University of Oslo Bioneer
The work done in the thesis has been partly reported in two scientific papers