fe mn - units.it · fe(ii) 2 ferricromo 3 desferriossamina enterobactina . midollo spinale dcytb...
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![Page 1: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/1.jpg)
Fe Fe Mn Mn Zn Zn
Microtomografia di fluorescenza ai raggi X
Wild Type
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Ciclo del ferro
Chimica di Fenton Fe(II) + 3O2 Fe(III) + O2·
–
Fe(II) + H2O2 Fe(III) + OH– + OH·
[Fe(III)] = 10-17
![Page 3: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/3.jpg)
desferricromo
desferriossamina
Siderofori
Idrossammati (funghi, lievito)
Fe(III) alto spin
![Page 4: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/4.jpg)
FeC
N N
C
C
N
-N-cis,cis
FeN
C N
C
N
C
-C-trans,cis
FeC
C N
C
N
N
-N-trans,cis
FeN
N N
C
C
C
-C-cis,trans
FeC
N C
N
C
N
-N-trans,cis
![Page 5: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/5.jpg)
Siderofori catecolati (batteri)
Enterobactina Complesso V(IV)-enterobactina
D
Fe(III) alto spin
![Page 6: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/6.jpg)
Fe Insolubile
1
Riduzione
Fe(II)
Esterno della Cellula Membrana Interno della Cellula
SOLUBILIZZAZIONE TRASPORTO ASSIMILAZIONE
Idrolasi Fe(II)
Fe(II)2
3
ferricromo
desferriossamina
enterobactina
![Page 7: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/7.jpg)
Midollo spinale
DCYTB
DMT1
ferrochelatasi
ferroriduttasi
pompa
10–30 mM
![Page 8: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/8.jpg)
Transferrina 80 kDa
![Page 9: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/9.jpg)
Sito di coordinazione del Fe nella transferrina
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Sito di coordinazione del Fe nella transferrina
![Page 11: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/11.jpg)
endocitosi
Ciclo della transferrina
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Ferritina
24 unità
440 kDa
8 pori idrofilici con simmetria ternaria (Fe in)
6 pori idrofobici con simmetria quaternaria (Fe out)
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In, 7.5nm
Out, 12 nm
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Fino a 4500 atomi di Fe
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sito ferrossidasico
nella H-ferritina
![Page 16: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/16.jpg)
Sito attivo
Fe(III)2O
nucleation channel
sito ferrossidasico
affinità bassa, 15 mM
![Page 17: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/17.jpg)
Struttura ai raggi X di un sito ferrossidasico
![Page 18: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/18.jpg)
Immagine
TEM
![Page 19: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/19.jpg)
Rilascio del ferro
![Page 20: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/20.jpg)
Omeostasi del ferro – Sistema IRP/IRE
IRP: Iron Regulatory Proteins
IRE: Iron Responsive Elements (mRNA)
ferroriduttasi
pompa Fe2+
ceruloplasmina
epcidina
PCBP1
ERRORE!
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Sistema IRP/IRE
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Export del ferro nel plasma regolato da epcidina
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Omeostasi del rame
Trans-Golgi Network
ATP7A e
ATP7B
![Page 24: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/24.jpg)
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amb. riducente
amb. ossidante
![Page 26: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/26.jpg)
CCS/Zn-SOD
Sito di binding del Cu(I)
![Page 27: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/27.jpg)
CCS/Zn-SOD
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Atx1/Ccc2
![Page 29: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/29.jpg)
Sensori del Cu
Fattore di trascrizione CueR (da E. Coli)
K ≈ 1021
![Page 30: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/30.jpg)
Omeostasi dello zinco in cellule eucariote
= pompe ZIP
= pompe CDF
![Page 31: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/31.jpg)
Proteine regolatorie
Omeostasi dello zinco nei batteri
zinc-responsive proteins
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Fattore di trascrizione ZntR
![Page 33: Fe Mn - units.it · Fe(II) 2 ferricromo 3 desferriossamina enterobactina . Midollo spinale DCYTB DMT1 ferrochelatasi ferroriduttasi pompa 10–30 mM . ... Trans-Golgi Network ATP7A](https://reader033.vdocuments.us/reader033/viewer/2022051806/60014bceb1cae81cce085526/html5/thumbnails/33.jpg)
Metallotioneine
6 kDa,
ca. 60 a.a.
fino 30% cys
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Metallotioneine
CdZn2S8 Cd4S10