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$: EXPERIENCE SUPERIOR X-RAY DATA QUALITY - … · Single-crystal X-ray diffraction systems from Agilent Technologies provide crystallographic data of the highest quality. With more$
EXPERIENCE SUPERIOR

X-RAY DATA QUALITY

Agilent X-ray Crystallography Systems

Single-crystal X-ray diffraction systems from Agilent Technologies provide crystallographic data of the

highest quality. With more than 20 years of experience designing and manufacturing all major components,

Agilent has a proven history of expertize in providing innovative systems for both chemical crystallography

and structural biology.

AGILENT X-RAY CRYSTALLOGRAPHY SYSTEMS

EXPERIENCE SUPERIOR X-RAY DATA QUALITY

All Core Technologies In-house

Agilent X-ray systems are designed as

complete units, with all main components

manufactured by Agilent. With precision

mechanics, electronics, X-ray sources, CCD

detectors and software — all engineered

specifi cally for crystallography — we focus

on providing fully integrated solutions for

single-crystal X-ray diffraction.

X-ray SourcesPrecision Mechanics

Agilent Gemini System Agilent PX Scanner System Agilent SuperNova System

2

X-ray User Community Forum

Get the very best from your system by

staying in close contact with both Agilent

experts and users around the world.

• Access to hundreds of small molecule

and protein crystallographers

• Be the fi rst to hear about new

software updates

• Join in with discussions and debates, ask

(or even answer) technical questions and

benefi t from the collective experience and

expertise of a large and active community

• Available to all Agilent system users at

www.agilentxrdforum.com

Centers of Excellence

You are specially invited to visit and

collect data at one of our X-ray Centers

of Excellence worldwide...

or send us a sample and we’ll collect

data for you!

SoftwareDetectors

X-ray Center of Excellence, Santa Clara, CaliforniaX-ray user community forum

3

High pressure

Service DisorderAbsolute structure

Extended inorganics

Absorbing materials

Small weak crystals

Twinning Macro-molecules

Applications

From the inventors of dual-source technology,

Agilent Technologies offers a full range of

small molecule systems for all applications

and budgets. Combining precision hardware

with user-inspired crystallographic software,

Xcalibur, Gemini and SuperNova systems

demonstrate Agilent’s continued innovation

and leadership in chemical crystallography.

Benefi ts of the Xcalibur

and Gemini

• Superior affordability with no

compromise in data quality

• Gemini — dual-source confi guration

(Mo & Cu) for the complete range

of applications

• Exchangeable collimators for

fl exible X-ray beam size

• Fully automated with CrysAlisPro

and optional AutoChem2.0

• Hardware and software update

reports by email — stay in touch

with your system wherever you

happen to be

• Automated workfl ows or manual

control — perfect for new and

advanced users

Xcalibur and Gemini

The traditional sealed tube single-source

Xcalibur and dual-source Gemini systems

offer Agilent’s most affordable single-crystal

diffractometers.

Features

• Single or dual (Gemini) fi ne-focus

Enhance X-ray sources

• Select our fast readout CCD detectors

for highest sensitivity (Eos) or large

active area (Atlas)

• CrysAlisPro with optional AutoChem2.0 —

user-inspired software for superior data

quality and automation

Xcalibur with Enhance X-ray source and Eos CCDComplete Gemini A Ultra system with external

X-ray chiller


SUPERIOR SYSTEMS FOR CHEMICAL CRYSTALLOGRAPHY

4

PowdersDiffuse scatter ProteinsModulated Structures

Charge / electron density

Quasi-crystals

SuperNova

The 3rd generation dual-source system

from Agilent, the SuperNova provides

highest quality data in the shortest time.

From service crystallography to charge

density, high pressure to absolute

confi guration, the SuperNova is the

ideal multi-purpose system for chemical

crystallography laboratories.

Features

• Low power (up to 50 W), high fl ux single

or dual micro-focus X-ray sources, with

3-year warranty available

• NEW — brightest ever 2nd generation

Nova source

• Select our fast readout CCD detectors

for highest sensitivity (Eos) or large

active area (Atlas)

• CrysAlisPro with optional AutoChem2.0 —

user-inspired software for superior

data quality and automation

• Compact, self-contained, modular system

Added benefi ts of the

SuperNova

• The power of the micro-focus

Nova (Cu) source coupled with

CrysAlisPro’s supreme ability for

processing Cu data — see how

Cu has become the wavelength

of choice for most dual-source

system users

• Faster, stronger data in less time

• Measure samples you could never

measure before — The Nova (Cu)

source provides excellent data

from tiny, weakly diffracting crystals

and twins

• AutoChem2.0 (optional) — Increase

your productivity, with speed of data

collection and automatic structure

determination

• Compact footprint and designed

for supreme reliability and

serviceability

Dual-source SuperNova goniometer with Atlas CCDComplete SuperNova system including table for

keyboard and monitor

Goniometer and upgrades

• Agilent diffractometers include a high

precision 4-circle kappa goniometer for

maximum data coverage and the best

optimized data collection strategies

• All single-source diffractometers can later

be upgraded to a dual source system

5

SuperNova applications

SAD or Heavy Atom Phasing

Molecular Replacement (MR) RNA/DNALigand bindingScreening

SuperNova

A compact, sealed tube micro-focus

system providing high quality data

with high intensity, high reliability and

maximum uptime.

Features

• NEW — brightest ever, 2nd generation

micro-focus Nova X-ray source, with

3-year warranty available

• Eos, Atlas or largest active area Titan

(165 mm) CCD detectors

• CrysAlisPro PX — dedicated, user-friendly

software for macromolecular data

collection and processing


The Agilent SuperNova and PX Scanner

provide a range of unique capabilities

and benefi ts to meet the needs of

modern structural biology facilities.

From crystallization screening to full data

collection, our compact, cost-effective

and user-friendly systems offer the ideal

in-house solution to complement the

usage of synchrotron beamlines.

Benefi ts of the SuperNova

• The ideal in-house system to

complement synchrotron visits

• Rapid crystal screening or full

data collection

• Sealed-tube X-ray source —

no moving parts for supreme

reliability and serviceability

• Hardware and software status

reports by email — stay in touch

with your system wherever you

happen to be

• Low cost-of-ownership —

for both day-to-day running costs

and service contracts

• Compact and self-contained

SuperNova with Nova X-ray source and Titan CCDComplete SuperNova system including table for


SUPERIOR SYSTEMS FOR STRUCTURAL BIOLOGY

6

PX Scanner applications

Small, fragile crystalsOptical and in-situ screening Screen sensitive, membrane proteins

Comparison of crystal diffraction quality

Inserting a multi-well plate into the PX Scanner (top)

with the CrystalEyes software platform used in optical

(middle) and X-ray (below) screening

PX Scanner

A unique instrument for optical and X-ray

screening of protein crystals, in-situ in the

multi-well crystallization plate. Combining

optical and X-ray imaging, the PX Scanner

allows for visualisation, identifi cation and

X-ray evaluation of protein crystals

undisturbed within the growth media.

Features

• Vertically-mounted 2nd generation

micro-focus Nova X-ray source

• 135 mm active area CCD detector

• Touch-screen control and barcode reader

for plate identifi cation

• Up to 6° tilt for scanning X-ray images

• Accepts virtually all SBS-format plates

• CrystalEyes — user-friendly software

for all optical and X-ray screening plus

secure data storage

Benefi ts of the PX Scanner

• Early stage identifi cation of

crystalline material

• Discrimination of salt from

protein crystals

• Identifi cation of optimal crystal

growth conditions

• Comparison of crystal

diffraction quality

• Automated crystal screening

leading to accelerated results

• Study of crystals too small and

fragile to extract

Complete PX Scanner system including table for


7

AGILENT CrysAlisPro SOFTWARE

Agilent’s comprehensive crystallographic

data collection and data processing

software, CrysAlisPro is designed as a

user-friendly interface for both small

molecule and protein confi gurations. Simply

select between SM and PX modes for fully

optimized workfl ows and data processing

routines. Frequent updates offer every user

access to the very latest developments and

we encourage feedback for incorporating

as many user ideas as possible into new

software releases.

• Free multi-user, multi-site license

• Free regular updates, with new

user-inspired features

CrysAlisPro SM

For small molecule samples, CrysAlisPro

combines automated crystal screening, the

fastest, most accurate strategy software

available, concurrent data reduction and

automatic structure solution and refi nement

with AutoChem (optional), giving you visual

results in the shortest time possible.

• GUI or command line control

• Super-fast pre-experiments to determine

crystal quality and data collection strategy

• CellCheckCSD — developed in

collaboration with the CCDC — free

access to cell information from the

Cambridge Structural Database

• Hundreds of specialist crystallographic

tools — including powder, twin,

incommensurate and high pressure

modules

• Import and process frames from

synchrotrons and other frame formats

EwaldPro — Reciprocal Lattive Viewer

Crystal face-indexing tool

CrysAlisPro SM — with integrated AutoChem feedback panel

CRYSALISPRO — USER-INSPIRED SOFTWARE

FOR SUPERIOR DATA QUALITY

8

CrysAlisPro PX

A simple software switch confi gures

CrysAlisPro for macromolecular crystal

screening, data collection and data

reduction. Dedicated protein features

include:

• Data collection, processing and

scaling in a single package

• Crystal screening tool for testing

crystals in quick succession

• Strategy calculation, processing

and scaling algorithms optimized for

macromolecular crystallography

• Rapid multi-core data processing

• Image analysis: d-value and resolution

assessment tools

• Import and process data from

synchrotrons, image plates and

other CCDs

• Export data to MosFLM, XDS and

HKL formats

CrysAlisPro PX — Dedicated protein confi guration

AutoChem2.0

• Fully automated structure solution,

refi nement and report generation, with

real-time feedback during data collection

• Automated use of multiple solution

programs* (direct methods, Patterson

and charge fl ipping) for the best chance

of success

• Full user interaction and intervention

possible at anytime in Olex2

• AutoChem is exclusive to Agilent

Technologies

*depending on locally installed programs and licences

AutoChem main view and solution and refi nement

settings panel

9

Cryo-devices

• Xcalibur, Gemini and SuperNova systems

accept all commonly-available cryo- and

heating-devices.

• Includes Cryojets, Cryostreams, Cobras

and the Desktop Cooler (DTC), as well as

Agilent’s open-fl ow liquid helium cooling

device, the Helijet (<15-75 K).

• Full software control of cryo-devices -

including CrysAlisPro’s multi-temperature

experiment feature.


COMPACT, FULLY INTEGRATED SYSTEMS —

TUNED TO YOUR NEEDS

The Agilent Helijet open fl ow cooling device

SuperNova system with AirCooler radiator unit

Modular Design

• Individual modules designed for simple

service and support

• Quick and easy replacement means any

downtime is minimized

Inside view of the SuperNova base cabinet

Compact, Standalone

Systems

• 0.5 m2 footprint, making SuperNova and

PX Scanner systems ideal in laboratories

where fl oor space is limited or expensive

• Low power micro-focus X-ray sources -

Addition of an AirCooler radiator makes

both systems entirely self-contained

• Low power X-ray sources operate through

a standard mains power supply

10

2005Gemini System

2005Gemini Ultra

2008SuperNova

2005Gemini co-mountedenhance X-ray sources

2011> 100 dual wavelength

systems worldwide

2008Co-mounted Nova (Cu) and Mova (Mo) X-ray Sources

2006 2007 2008 2009 2010


DUAL SOURCE — PROVEN SUCCESS

Agilent Technologies (formerly as Oxford Diffraction) installed the world’s fi rst dual-source diffractometer, the

Gemini, in 2005. Developments in focusing optics and micro-focus sources makes the current generation of

dual-source systems more powerful than ever. Together, the Gemini, Gemini Ultra and SuperNova systems

represent Agilent’s continued innovation and leadership in dual-source diffractometer design.

Co-mounted Nova and Mova dual micro-focus X-ray sources11

Learn more

www.agilent.com/chem/xrd

Contact Agilent’s X-ray Crystallography sales team

[email protected]

Find your local Agilent customer center

www.agilent.com/chem/contactus

U.S. and Canada

1-800-227-9770

[email protected]

Europe

[email protected]

Asia Pacifi c

[email protected]

This information is subject to change without notice.

© Agilent Technologies, Inc. 2012Published in the USA, April 20, 20125991-0061EN

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