everything you ever wanted to know about dna collections and … · 2016. 4. 13. · everything...
TRANSCRIPT
4/13/2016
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Everything you ever wanted to know about
DNA collections and MORE!
Presented by: Kim Levaggi
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Accreditations
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50/50• Half of your DNA comes from your mother
• Half of your DNA comes from your father
Child
A B15 13
Mother
A B12 15
Father
A B12 13
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Relationship Tests
• Paternity
• Maternity
• Grand Parentage
• Avuncular (aunts and uncles)
• Siblingship– Half vs. Unrelated
– Half vs. Full
– Full vs. Unrelated
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Siblingship Tests
• Half vs Full (Know they share one common parent
and want to determine if they share both)
• Half vs Unrelated (test to determine if they share
one common parent)
• Full vs Unrelated (think they share both or neither)
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Chain Of Custody form (COC)
• Paternity
• Maternity
• Grand Parentage
• Avuncular
• Siblingship
• Y STR
• Infidelity
• Ancestry
• Other
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Who should be tested and why?
• Always include mom if possible (all relationship
tests)
– Reduces the chances of inconclusive results
• Approximately 3% of cases without the mother result in
inconclusive results
– No additional charge if done with original test
– Additional charge if added at a later date1.Additional shipping costs
2.Perform the test for the mother
3.Recalculate statistics
4.Reissue and review another report
5.A minimum of 2 additional business days for results
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What if Mom is not available?
• Results can be achieved with alleged father only
– Approximately 97% of tests are conclusive
without mom
• Extended markers
– 16 markers standard
– Our Standard test is 24 markers
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Paternity/Kinship Testing
Father not available (options)•Grand Parentage (paternal)
•Avuncular (aunts / uncles)(paternal)
•Siblingship
– Half vs Unrelated
– Half vs Full
– Full vs Unrelated
Include the mother(s)
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Relationship average probabilities
Test Scenario Tested IndividualsTypical Probability
Support of
Evidence Typical Probability Support of Evidence
16 Markers 16 Markers 24 Markers 24 Markers
Avuncular
(Aunts and Uncles)
Child and Alleged Aunt 91.67% Moderate 98.21% Moderate
Child and Alleged Uncle 91.67% Moderate 98.21% Moderate
Child, Mother and Alleged Aunt 95.45% Moderate 99.34% Strong
Child, Mother and Alleged Uncle 95.45% Moderate 99.34% Strong
Grandparentage
Child and 1 Alleged Grandparent 83.05% Limited 95.83% Moderate
Child, Mother and 1 Alleged
Grandparent 97.78% Moderate 99.34% Strong
Child and 2 Alleged Grandparents 99.85% Strong 99.99% Very Strong
Child, Mother and 2 Alleged
Grandparents 99.99% Very Strong >99.999% Very Strong
Siblingship
(Full vs. Half)
Child A and Child B 92.86% Moderate 97.44% Moderate
Child A, Child B and Mother 99.78% Strong 99.92% Very Strong
Siblingship
(Half vs. unrelated)
Child A and Child B 90.91% Moderate 95.45% Moderate
Child A, Child B and Mother A 96.67% Moderate 99.53% Strong
Child A, Child B, Mother A and B 99.24% Strong 99.92% Very Strong
Siblingship
(Full vs. Unrelated) Child A and Child B 99.96% Very Strong >99.999% Very Strong
MaternityChild and Alleged Mother 99.99% Very Strong >99.999% Very Strong
Child, Father and Alleged Mother >99.999% Very Strong >99.999% Very Strong
PaternityChild and Alleged Father 99.99% Very Strong >99.999% Very Strong
Child, Mother and Alleged Father >99.999% Very Strong >99.999% Very Strong
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• Verification of Identity Not mandatory
• Names Not mandatory
– Father, Child A, Child B
• Dates of Birth Not mandatory
• Consent signatures ** Not mandatory
• Collectors name & signature Not mandatory
• Photos Not mandatory
• Maintaining Chain of Custody Not mandatory
Self collection is permitted
** Recommended
Private DNA Collection
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• Identify each individual prior to starting
– Government issued ID
• Birth Certificate/hospital documentation for child
• Use clean pair of latex/nitrile gloves for
collection
• Fill out information on Chain of Custody (COC)
• Fill out the information on ONE sample envelope
for ONE person
I recommend preopening the sample envelope
Legal DNA Collection
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Chain Of
Custody
COC
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• Open a package of swabs
• Swab between cheek and gum
– Gently rub/twirl for 20-30 sec.
• 2 swabs per person
– 1 on the right, 1 on the left side
• Place each swab in the sample envelope
Swab end first
• Seal the envelope
Legal DNA Collection (cont.)
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• Obtain consent signatures/Initials on COC and
sample envelope
– Mother typically signs for child
• Collector must print and sign his/her name
– Multiple collectors, must have multiple signatures
• Complete each person prior to moving on
• Repeat the process for each individual
• Following the order on COC will reduce errors
– Mother
– Child
– Alleged Father
DNA Collection (cont.)
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• Collection site on COC must be correct
– Pre-printed top of our COCs
– Important that info matches surroundings in photo
• Foreign names are occasionally written
backwards. We follow exactly as written on COC
– Additional charge to correct misspelled names
1. Corrections made to file
2. Report reprinted
3. Report re-reviewed by 2 individuals
4. Re-notarized
5. Re-issued
DNA Collection (cont.)
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• Photos can be taken with
– Digital Camera
– Regular Camera
– Phone
• Photos can be emailed to us at:
– Subject should contain name of the individual in the
photo
– Body should reference date samples being shipped
• Numerous individuals can be in a single photo
– Subject line L to R: John Doe, Jim Doe, Jane Doe
DNA Collection (Photos)
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• Identify the individual in
the photo
• Photos may be taken
with client holding
a name board
• If using photo labels
please DO NOT place label
over the face of the
individual
DNA Collection (Photos)
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• Must be identifiable
Too light Too Dark Just Right
If the photo is questionable. RETAKE IT!
DNA Collection (Photos)
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• Photos of baby’s face
– Need front facing photo
Unacceptable Acceptable
DNA Collection (Photos)
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• Copy of Driver’s license is sufficient if legible
and matches clients present appearance
– Occasionally B/W copies are not acceptable
– Color copies may improve image
Believe it or not we get these
Unacceptable Acceptable
DNA Collection (Photos)
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PHOTO QUESTIONABLE?
PLEASE RETAKE IT!
DNA Collection (Photos)
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PaternityChain of
Custody or
COC
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• Place all applicable items in the courier envelope
– Sealed sample envelopes
– All photos or copies of IDs
– COC(s)
– All associated documents
• Court orders
• Dr. Scripts, attorney letter (for NY)
• Third party authorization form
• Record the tracking # from the courier envelope
• Give to Courier for shipment to the lab
DNA Collection (completion)
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Non-Legal vs Legal summary
Non Legal Legal
ID Verification No Required
Photos No Required
Consent ** No Required
Date/Time No Required
Collection location No Required
Chain of custody No Required
Non Legal cases can be self collected
** Recommended 25
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What is a DUO and a TRIO
DUO TRIOChild Child
Alleged Father Alleged Father
Mother
Additional alleged father or an additional child
is NOT a trio26
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Out of State Collection Sites
Mother and child in your city, state
Alleged father in another city, state
No problem!
• On-line portal 24/7
• Thousands of collection sites in the US
• www.mediprodirect.com
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Collection Procedure (out of state)
1. Contact collection site to schedule appointment
2. Confirm
• They have our kits
• The collection site address
• The collection fee
• Their payment requirements
• They are capable of taking a photo
3. Schedule the appointment
4. Fax/email them, your completed COC
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Collection Procedure (out of state)
5. State in your fax or email to please use the
attached COC for the collection
• Make certain you check the Legal or Informational
box so they know to take photos and get signatures
• List all names on the COC
6. Confirm in you email or fax who they are to
collect
• Write the person’s name and date of collection of the
out of state collection in the supplemental
information area on the COC you are sending to the
lab so the lab can match them up
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Please Use our
Website portal
or
PRINT CLEARLY
DNA Collection (cont.)
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Website Portal
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Benefits of our website portal
• Create a case with an assigned case #
• Order kits
• Locate a collection site
• Track your case status
• Add you personal logo to your report
• Select from 6 customized reports
• Provides access to our DNA Resource Guide
• Provides your up-to-date pricing
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Instructions to generate a case
• Log on to website with username and
password
• Create a case
• Select type: Paternity, Siblingship, Etc.
• Select Legal or Non-Legal
• Enter name of alleged father
• Order test
• Select download COC
• Enter participant’s names
• Print COC
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Standard Samples
Buccal swabs
Whole blood
FTA cards34
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Whole Blood
Vacutainer What is Contains Comments
Purple Top EDTA Useful for DNA
Yellow Top Acid Citrate and Dextrose Useful for DNA
Red Top No Additive Less useful
Gray Top NaF and sometimes EDTA May not be suitable
Ship how storedRoom Temp. (ship room temp or on blue ice)** Please note on COC
Refrigerated (ship on blue ice)
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Results Reporting
• Why does the paternity report say “can not be
excluded, instead of “is the father”?
– Cannot obtain 100% unless testing all men on
Earth
– Cannot be excluded• 10 men tested
– #1 - you’re excluded
– #2 - you’re excluded
– #3 - you’re excluded
– Etc.
– #10 – you cannot be excluded, meaning you are the father
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Test Results of 95% to 99% not an A+
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Example
Report
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Collecting Infants and Minors
• Collecting newborns
– No breast milk 2 hrs. prior to collection
• By breast or bottle
• Collecting minors
• Parent/Guardian must sign for underage
minor
You can also have the mother sign for the child
in addition to the parent/guardian
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Testing Deceased individual
• Exhumed body
• Expensive
• Non Standard: Bone (femur), teeth, fingernail clippings,
hair, tissue
• Embalmed body
• Teeth and bone samples are preferred
• Hair & nail samples can be difficult to obtain a full profile
• Buccal swabs and tissue samples are not recommended
• Recently deceased (not buried or cremated)
• Buccal swabs (distilled water)
• Non Standard: Blood, fingernail clippings, hair (with root
or follicle) 40
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Alleged Father (Recently deceased) Legal
• Questions to ask:– How long ago did he/she pass away?
– Is his body available to collect samples?
– Where is the body?
• Funeral home
• Morgue
– Can I gain access to the body?
– Who is the next of kin?
• Wife/mother
• Are they willing to give consent?41
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• Materials needed– Gloves
– Small bottle of distilled water
– Fingernail clippers or scissors
– Alcohol
– Lighter or matches
– DNA collection kit
– Affidavit COC or Affidavit of Identity Form
– Authorization to compare form
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Alleged Father (Recently deceased) Legal
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For a legal relationship tests, AABB requires a photograph or copy of a government issued photo ID for
proper identification of individuals who were collected. In very rare circumstances (i.e. samples
provided by law enforcement agencies, coroner’s samples or prenatal samples) where identification
cannot be verified via a photograph, a signed affidavit from the individual(s) identifying and collecting
the samples needs to be provided.
AFFIDAVIT OF IDENTITY
Name of individual sampled ___________________________________
Date of Biological Specimen collected ___________________________
Collection Location __________________________________________
This is to certify that the above named individual was presented for a biological specimen collection on
the date documented above.
I understand that this document may be used in a court of law for verification of identity and I may be
called upon to testify as to its validity.
Date___________________________
Name of Person Verifying Identity (print)____________________________________________________
Title _________________________________________________________________________________
Signature of Person Verifying Identity ______________________________________________________
Date __________________________
Name of Collector (print)________________________________________________________________
Signature of Collector ___________________________________________________________________
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Deceased Legal Collection Procedure
Prior to leaving for collection
– Gather all materials needed for collection
– Dip fingernail clippers/scissors in alcohol
– Ignite the lighter or match and flame the alcohol off
– Place the clipper/scissors in a sample envelope
– Fold over flap
Collection
– Provide information on 3 sample envelopes
• Buccal swabs
• Fingernail clippings
• Hair44
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Deceased Legal Collection Procedure
1. Remove swab from package and dip in distilled
water, shake to remove excess water
2. Swab between check and gum 20-30 secs.
3. Repeat with remaining swab
4. Place in envelope and seal
5. Remove nail clipper/scissors from envelope
6. Hold envelope under nails and clip 4-5 nails
7. Seal envelope
8. Pull 15 – 20 hairs and place in 3rd envelope
9. Seal envelope 45
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Identification of individual is required
–Take photo of deceased individual if possible
–Take photo of ID bracelet or toe tag
–Funeral Director/Coroner must complete and
sign affidavit of Identity
–Photo of individual not required if affidavit is
signed
Authorization to compare consent form signed
by next of kin46
Deceased Legal Collection Procedure
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Alleged Father (Deceased) Legal
Body not available to collect samples?
Is blood sample or tissue sample available at the
M.E./Coroner or hospital?Yes –Ship it to lab
Ship how stored (if stored properly)
Cost of shipping is client’s responsibility
No - What other family members are available for testing?
Grandparents, Siblings, Aunts or Uncles
Mother of the child?
Always include mother in all tests
Use relationship COC
Collect all parties and ship to lab
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Non Standard Samples
• Teeth, Organs & tissue – Advance Non-standard
• Hair, Fingernails, Toothbrush, hairbrush, clothing etc.
– Basic Non-standard
• Blood stains on anything other that FTA card (blood
card) = Basic Non-standard
• Whole blood (vial) No additional charge
• FTA card (blood card) No additional charge
• 3-5 business days on all above items
• Bone = Non standard (5-10 business days)
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– Gum
– Bone
– Toothbrush
– Cigarette butts
– Jewelry
– Hairbrush
– Clothing• Hat
• Bandana
Non-Standard Samples
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What records should I keep for my file?
That’s your decision• We maintains your case records for 5 years
7 years for NY cases
• Scanned copies of COC, photos, IDs are
kept on file
What’s returned to you?
•Test results only
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• No Private paternity testing
• No Infidelity testing
Only court admissible tests
Ancestry testing permitted in New York
What’s allowed in New York?
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Who MUST
request a
DNA test in
New York?52
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• Doctors– Prescription needed
– Must have all parties to be tested in NY listed
• Attorney – Letter requesting test needed
– Must have all parties to be tested in NY listed
• Court order– Must have all parties in NY listed
• Government (Immigration)
NY Paternity test must be ordered by:
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What Elements Are Most Detrimental To DNA?
Moisture
Bacteria
High Temperature
Sunlight
Soil54
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Actions to Avoid
• Avoid touching any areas/items where DNA will be tested
• Avoid sneezing, coughing, excessive talking while collecting DNA
• Avoid putting buccal swabs back in original package or in anything that is plastic or plastic lined
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Things to remember
• We always want DNA samples dry
– Moisture promotes bacteria. Bacteria degrades DNA
• If infants are receiving breast milk, wait 2 hrs. prior to performing the collection. Tell your client during initial communication
• To provide the best possible test for your client, find out all parties available for testing, then use our DNA RightTest to select the best test for them
• You can NEVER communicate too much information
– Use the supplemental information box on COC56
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• Complete one person at a time prior to moving on
• Always print clearly
• COC is our Bible! What is on it is what we use
• Photo Questionable? RETAKE IT!
• Doing it Right the first time makes for a happy customer
• First New York Case? CALL US
• Have a question? Call or email us!
We are here to support you and your staff
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Things to remember
