eukaryotic mrna processing alternative splicing cap and tail exon intron exon intron exon dna...

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Eukaryot ic mRNA processi ng Alternativ e splicing Cap and Tail Exon Intron Exon Intron Exon DNA Cap Transcription Addition of cap and tail RNA transcript with cap and tail Introns removed Tail Exons spliced together mRNA Coding sequence Nucleus Cytoplasm

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Page 1: Eukaryotic mRNA processing Alternative splicing Cap and Tail Exon Intron Exon Intron Exon DNA CapTranscription Addition of cap and tail RNA transcript

Eukaryotic mRNA

processing

Alternative splicing

Cap and Tail

Exon Intron Exon Intron Exon

DNA

Cap TranscriptionAddition of cap and tail

RNAtranscript with capand tail

Introns removedTail

Exons spliced together

mRNA

Coding sequence

Nucleus

Cytoplasm

Page 2: Eukaryotic mRNA processing Alternative splicing Cap and Tail Exon Intron Exon Intron Exon DNA CapTranscription Addition of cap and tail RNA transcript

Alternative Splicing of Exons

mmnvnavyakcvtpdeavtlitsgshlsgmfaaeppallnalakrakr*

mmnvnavyakcvtpdgmfaaeppallnalakrakr*

eavtlitsgshlsgmfaaeppallnalakrakr*

Can result in related proteins

This version has all exons included:

These versions have different exons included in the final verison of the protein

Page 3: Eukaryotic mRNA processing Alternative splicing Cap and Tail Exon Intron Exon Intron Exon DNA CapTranscription Addition of cap and tail RNA transcript

Alternative splicing makes it possible for a single gene to code for a number of different variations of a protein. Splicing patterns can be tissue-specific, so that a certain variation

of a protein is produced in a specific tissue.

Page 4: Eukaryotic mRNA processing Alternative splicing Cap and Tail Exon Intron Exon Intron Exon DNA CapTranscription Addition of cap and tail RNA transcript

Gene Mutations

Which is most harmful? Which is least harmful?What would happen if a base

was inserted?

Normal gene

mRNA

Base substitution

Base deletion Missing

Met Lys Phe Gly Ala

Met Lys Phe Ser Ala

Met Lys Leu Ala His

A U G A A G U U U G G C G C A

A U G A A G U U U A G C G C A

A U G A A G U U G G C G C A U

U

Protein

DECONVOLUTING BAC–GENE RELATIONSHIPS USING A ...stelo/papers/jbcb08.pdfGenes Unigenes exon intron probe Unigene Chromosome exon intron exon BACs BACs contig contig Fig. 1. An illustration

Conservation of Domain Structure in a Fast-Evolving ...sequenced the genomic DNA from the SuUR locus in D. erecta (GenBank accession no. AJ539550). The exon– intron structure of

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REVIEW Open Access Origin and evolution of spliceosomal ......Keywords: Intron sliding, Intron gain, Intron loss, Spliceosome, Splicing signals, Evolution of exon/intron structure,

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Departments Molecular Biology, Sciences Research, · cPosition is given as exon (E) or intron (I) no./codon no. Resultant amino acid changes are indicated in single-letter code. dlmmunohistochemical

April 6, 2010 14:28 WSPC/185-JBCB S0219720010004756 …web.eecs.umich.edu/~hero/Preprints/rao_JBCB10.pdf · · 2010-04-28April 6, 2010 14:28 WSPC/185 ... TATA box Exon Intron Distal

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tau Exon 10 Expression Involves a Bipartite Intron 10 Regulatory

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Global Proﬁling and Molecular ... - utoronto.casites.utoronto.ca/intron/Misquitta_et_al_2011.pdf · gaster) homolog (NUMB). Increased inclusion of exon 9 in transcripts from the

Plant-Derived Transfer DNAsSimilarly, Os01 deﬁnes the transi-tion from exon (5#-AGCCAGGATATATCCTTGA) to intron(5#-GTAAGT,withdonorsiteunderlined)ofthe rice cleavage stimulation factor

2000 by Cell Press Piccolo, a Presynaptic Zinc Finger Protein ...Piccolo, a Presynaptic Zinc Finger Protein 205 Table 1. Exon–Intron Boundaries of Human PCLO Gene Exon Encoded Exon

MUTATIONS, MUTAGENESIS, AND C A R C IN O G E N E S IS · Silent •Mis-sense •Nonsense •Frameshift Intron/exon boundaries (splicing) Control genes •• Promoter/enhancer sequences

Supplementary Figure S1. The exon-intron structure of the U22HG and GAS5 genes. (A) A comparative analysis of the U22HG gene in humans, frog, fugu, and

Intron correlations evolution intron/exonProc. Natl. Acad. Sci. USA Vol. 92, pp. 12495-12499, December 1995 Evolution Intronphasecorrelations andthe evolutionofthe intron/exon structure

GTCAGATGAGCAAAGTAGACACTCCAGTAACGCGGTGAGTACATTAA exon intron intergene Find Gene Structures in DNA Intergene State First Exon State Intron State

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BRCA1 Exon 11, a CERES (Composite Regulatory Element of ......Int. J. Mol. Sci. 2014, 15 13046 additional cis-acting elements (situated in the intron or exon) that can act either as

Modern Biology and Bioinformatics - bio.gnu.ac.krbio.gnu.ac.kr/lecture/bi/bi_intro_bi/Intro.pdf · Transcriptomics (전사체학): Exon-Intron 구조로 인하여 한개의 유전자가

Biochemical and molecular characterization of 3 ... · carboxylase 2, beta) genes was performed by PCR, fol-lowed by direct sequencing of all exons and exon-intron ... variations

Progress report Yiming Zhang 02/10/2012. All AS events in ASIP Intron retention Exon skipping Alternative Acceptor site NAGNAG AltA Alternative Donor

Open Research Onlineoro.open.ac.uk/52279/7/epigenomes-01-00018-v2.pdfrs734005 T > C, intron TC heterozygote Gastric cancer 0.799 0.642–0.995 0.045 [31] rs2302427 G > C, exon, missense

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Bioinformatics 1 -- lecture 22 - KSUfac.ksu.edu.sa/sites/default/files/genefindingeu.pdf · 2014-11-04 · Bioinformatics 1 -- lecture 22 Gene ﬁnding in eukaryotes!intron/exon boundaries!splicing!alternative