erm -bb501b – customer information · erm ®-bb501b – customer information dear customer thank...

Queens Road, Teddington, Middlesex TW11 0LY Tel: +44 (0)20 8943 7000 Fax: +44 (0)20 8943 2767 Web: www.lgcgroup.com

ERM®-BB501b – Customer information

Dear Customer

Thank you for your interest in ERM®-BB501b.

Recently we have been made aware that some units of this material have patches

of black discoloration on the surface of the meat. We have carried out tests on

some affected units and have established that the discoloration is not due to the

presence of bacterial or fungal growth. Analysis has not detected any significant

difference between affected and unaffected sachets for the properties of interest, so

the certified values remain valid. Examples of the appearance of sachet contents

are shown below:

Unaffected sachet Affected sachet

We have taken the decision to keep the material on the market to avoid a break in

supply to customers; however a project to make a new processed meat material is

underway.

When using the material, you are reminded to follow the instructions for use on

page 3 of the Certificate of Analysis which says to massage the sachet before

opening to mix the contents.

All request/queries should be directed to your local LGC Standards Sales office.

Details can be found on: www.lgcstandards.com

LGC July 2015

ERM®- BB501b

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4005

CERTIFICATE OF ANALYSIS ERM

®- BB501b

Processed meat – Proximates, chloride, hydroxyproline & metals

Constituent Number of laboratories Certified value 3,4 Uncertainty

5 k

5

Moisture 1 (g/100 g) 23 68.59 0.49 2.05

Nitrogen (g/100 g) 22 2.723 0.075 2.04

Total fat 1 (g/100 g) 21 11.57 0.44 2.03

Ash 1 (g/100 g) 22 2.998 0.041 2.02

Chloride (Cl) (g/100 g) 19 1.110 0.042 2.07

Hydroxyproline 2 (g/100 g) 13 0.197 0.014 2.04

Calcium (mg/100 g) 10 5.9 1.1 2.13

Iron (mg/100 g) 14 0.78 0.18 2.04

Magnesium (mg/100 g) 13 18.0 1.4 2.10

Phosphorus (mg/100 g) 14 278 15 2.09

Potassium (mg/100 g) 14 286 13 2.07

Sodium (mg/100 g) 18 873 40 2.05

1) The measurand is defined by the methods used by participant laboratories (see page 3).

2) Colorimetric method based on BS:4401-11:1995.

3) Unweighted median value of the mass fraction determined by participant laboratories. Each data set was obtained in a different laboratory and/or using a different method of measurement.

4) The results are traceable to the SI through the physical and chemical standards used by the participant laboratories.

5) The quoted uncertainty is the half-width of the expanded uncertainty interval. A coverage factor k, (approximately equal to 2 for all analytes) was calculated separately for each analyte, to give a level of confidence of approximately 95 %.

This certificate is valid for 12 months from the date of shipment provided the sample is stored under the recommended conditions.

The minimum amount of sample to be used for each analyte is based on the homogeneity assessment and is given on page 2.

NOTE

European Reference Material ERM®- BB501b was produced and certified under the responsibility of LGC

according to the principles laid down in the Technical Guidelines of the European Reference Materials® co-

operation agreement between BAM-LGC-IRMM. Information on these guidelines is available on the Internet (http://www.erm-crm.org).

Accepted as an ERM®, Teddington, June 2014.

Signed:

Dr Derek Craston, UK Government Chemist LGC Limited Queens Road Teddington Middlesex TW11 0LY UK

ERM® - BB501b

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Additional Information

Constituent Value 1 (mg/kg) Range (mg/kg)

Nitrate (as NO3) 16 9 – 34 2

Nitrite (as NO2) 1.3 0.6 – 3 3

Nitrate and nitrite levels were measured by participants in the inter-laboratory study, but the spread of the results received made them unsuitable for assigning certified values.

1) Median of inter-laboratory results 2) Range of inter-laboratory results 3) Range of inter-laboratory results excluding two high outliers

Calculated Value

Constituent Value (g/100 g) Uncertainty (g/100 g)

Sodium chloride (NaCl) 1 1.83 0.07

1) Calculated from the chloride content quoted on page 1

DESCRIPTION OF THE SAMPLE

The material was prepared using a commercial pork-based processed meat. After thorough mincing and mixing, it was sealed in retort pouches in 50 g portions and heat treated to sterilise. The material is stored at LGC at (5 ± 4) °C.

The material was tested for between-sachet homogeneity by analysing randomly selected samples from across the fill run. The homogeneity data for all analytes was examined and deemed as fit-for-purpose, with the exception of nitrate and nitrite. The weight of material taken for the homogeneity assessment of each analyte is shown in the following table and represents the minimum weight which should be taken for analysis.

Analyte Moisture Nitrogen Total fat Ash Chloride Hydroxyproline Nitrate

& Nitrite

Metals

Weight (g) 5 0.5 2.5 5 4 4 5 1

The uncertainty contribution from the homogeneity assessment was incorporated into the overall uncertainty figure.

INTENDED USE

This material is intended for use in the development, validation or quality control of analytical methods for the determination of the major constituents and selected additional analytes in meat and meat products. The methods used to provide the data for assigning the values to the material are summarised later in this certificate. The material may also be applicable to other matrices and procedures where suitable reference materials are not available.

ERM® - BB501b

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CERTIFICATION

This material has been certified by LGC using the results of an inter-laboratory exercise. The certified values are based on the median of laboratory medians following the elimination of results from laboratories failing to meet acceptable QC performance.

The uncertainty associated with each certified value was calculated by combining the characterisation uncertainty from the inter-laboratory exercise with the uncertainty calculated from the homogeneity study. This combined uncertainty was then multiplied by a coverage factor (k) calculated separately for each analyte according to the Welch-Satterthwaite equation for effective degrees of freedom.

ANALYTICAL METHODS USED FOR CERTIFICATION

Participating laboratories were free to choose any suitable method with which they were familiar, apart from the measurement of fat content, where an acid hydrolysis step was requested to ensure the measurement of bound as well as free fat. The methods used are summarised below.

Moisture Measured as loss on drying using a variety of conditions. Procedures included microwave drying, vacuum oven drying and hot air oven drying.

Nitrogen There were two approaches used by participants. Some used a Kjeldahl method (variety of catalysts) and others used instrumentation based on the Dumas principle.

Total Fat Laboratories used acid hydrolysis followed by solvent extraction (variety of solvents).

Ash All laboratories used dry ashing in muffle furnace. The end temperature varied between 425 °C and 600 °C.

Chloride Laboratories employed a variety of methods. Some used a method based on a coulometric titration, others used titration with a colorimetric end point. The preparation procedures included ashing and dissolution, cold or hot water extraction and acid extraction.

Hydroxyproline Samples were prepared by acid digestion (sulfuric or hydrochloric) followed by determination of hydroxyproline by chloramine ‘T’ oxidation and colorimetry using 4-dimethylaminobenzaldehyde.

Nitrate and nitrite Samples were extracted using hot water or hot borax solution. Nitrate and nitrite levels were measured using anion exchange HPLC with UV detection, or colorimetrically mainly using sulfanilamide and naphthylethylenediamine hydrochloride, measured at a wavelength of 538 nm.

Calcium, iron, magnesium, phosphorus, potassium and sodium

Laboratories used a variety of methods including ICPOES, ICPMS and AA.

SAFETY INFORMATION

Refer to safety data sheet.

INSTRUCTIONS FOR USE

Immediately prior to analysing the material, massage the sachet gently to mix the contents, paying particular attention to the corners, and then open carefully. Transfer the entire contents to suitable mixer or blender and homogenise thoroughly. Store in an airtight jar and treat as fresh meat.

STORAGE

The material should be stored at (5 ± 4) °C in the original sealed sachet. Once opened, the sample should be treated as fresh meat.

ERM® - BB501b

Page 4 of 5


PARTICIPANTS

The number of participants’ results used in the calculation of the certified values is given in the table on page one. The following organisations took part in the inter-laboratory exercise:

Ashwood UK Ltd UK

Beverley Analytical Laboratories Ltd UK

Birmingham City Laboratories UK

BRULABO Belgium

Cargill Meats Europe UK

Chem-Science Laboratories (Pty) Ltd South Africa

Eurofins Food Testing UK Ltd UK

Glasgow Scientific Services UK

ILS Limited UK

Lancashire County Scientific Services UK

Land O’ Lakes USA

LATU Uruguay

LGC UK

Microsearch Laboratories Ltd UK

Noon Laboratories UK

Premier Analytical Services UK

Salamon & Seaber Limited UK

Sciantec Analytical Services UK

SILLIKER SAS France

Staffordshire County Council Scientific Services UK

State Veterinary & Food Institute Slovakia

SVA Ltd UK

Syni Laboratory Service Ltd Iceland

ERM® - BB501b

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Unit Number: Shipment Date:

Legal Notice

The values quoted in this certificate are the best estimate of the true values within the stated uncertainties and based on

the techniques described herein. No warranty or representation, express or implied, is made that the use of the product

or any information, material, apparatus, method or process which is the subject of or referred to in this certificate does

not infringe any third party rights. Further, save to the extent: (a) prohibited by law; or (b) caused by a party's

negligence; no party shall be liable for the use made of the product, any information, material, apparatus, method or

process which is the subject of or referred to in this certificate. In no event shall the liability of any party exceed

whichever is the lower of: (i) the value of the product; or (ii) £500,000; and any liability for loss of profit, loss of business

or revenue, loss of anticipated savings, depletion of goodwill, any third-party claims or any indirect or consequential loss

or damage in connection herewith is expressly excluded.

Production of this reference material and certificate was funded by the UK National Measurement System.

This certificate may not be published except in full, unless permission for the publication of an approved extract has been obtained in writing from LGC Limited. It does not of itself impute to the subject of measurement any attributes beyond those shown by the data contained herein.

erm -bb501b – customer information · erm ®-bb501b – customer information dear customer thank...

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