ER+ ER- ER+ ER-

This study Terunuma et al JCI 2014Supplemental Figure 1

gamma-glutamylisoleucine*

Normal ER+ ER-0

1

2

3

4

5

glutathione, reduced (GSH)

Normal ER+ ER-0

2

4

6

8

10

12

isoleucine

Normal ER+ ER-0

0.5

1

1.5

2

2.5

glutamate

Normal ER+ ER-0

0.5

1

1.5

2

2.5

3

3.5

glutathione

GCS

GS

γ-glutamyl-amino acid

amino acid

amino acid

5-oxoproline

outside cell

GGT

cys-gly

glutamate

glycine

cysteine

redox homeostasis

inside cell

Supplemental Figure 2

IDH1 R132 IDH2 R140 IDH2 R172

TCGA-B6-A1KF

HT1080

A.

B.

Supplemental Figure 3

0.0 0.5 1.0 1.5 2.00

5

10

15

20

D-2-HG (PK lab)

2-H

G (

Met

abo

lon

)

0 5 10 15-50

0

50

100

150

200

250

D-2-HG (PK lab)

2-H

G (

Met

abo

lon

) p<0.00001R=0.9987

p<0.00001R=0.9674

Supplemental Figure 4

mRNA - BRCA1 and fatty acid synthesis genes

Supplemental Figure 5

P<0.0001 P=0.0002

P=0.0002 P=0.0013

P=0.0001P=0.02

P=0.02P=0.01

Supplemental Figure 6

Supplemental Figure 7

Supplemental Figure 8

P=0.007

P=0.05

P=0.02

P=0.03