enzymes. enzyme active site amino acid side chains interact, metal ions, various types of polar,...
TRANSCRIPT
Enzymes
Enzyme Active Site• Amino acid side chains interact, metal ions,• Various types of polar, non-polar, ionic interactions
Enzyme Active Site• Amino acid side chains interact, metal ions,• Various types of polar, non-polar, ionic interactions
Enzymes• Lock and Key Analogy: lock = enzyme, key = substrate.
Enzymes• Lock and Key Analogy: lock = enzyme, key = substrate.
Induced Fit Theory• Enzyme is not rigid, changes shape with substrate.
Induced Fit Theory• Enzyme is not rigid, changes shape with substrate.
Enzymes - Cofactors• Coenzymes (contain a vitamin) or metal ion
Enzymes - Cofactors• Metal ions present in trace amounts
Enzymes - Cofactors• Metal ions present in trace amounts
Enzyme Cofactors• Coenzyme - non protein organic, maybe a vitamin.
Enzyme Cofactors• Coenzyme - non protein organic, maybe a vitamin.
Enzyme Cofactors• Coenzyme - non protein organic, maybe a vitamin.
Enzymes
Enzymes - Activity• Temperature and pH effect enzyme action
Enzymes - Activity• Temperature and pH effect enzyme action
Enzymes - Activity• Enzyme and substrate concentrations
Enzymes - Inhibitors• Nonspecific - denaturation, pH, temperature
Enzyme Inhibitors• Noncompetitive
Enzyme Inhibitors• Competive - mimic substrate, may block active site, but may
dislodge it.
Enzyme Inhibitors• Competive - mimic substrate, may block active site, but
may dislodge it.
Enzyme Inhibitors
Enzyme Inhibitors• Irreversible - covalently binds to active site
Enzyme Inhibitors• Regulator or feedback - used to control a sequence of reactions - reaction product may block initial enzyme.
Enzyme Inhibitors• Regulator or feedback - used to control a sequence of reactions - reaction product may block initial enzyme.
Enzyme Inhibitors
Enzyme Inhibitors
Drugs as Enzyme Inhibitors• Drug mimics some natural chemical to block enzyme.
Drugs as Enzyme Inhibitors• Drug mimics some natural chemical to block enzyme.