Raman Spectroscopy Technology

Enwave Optronics, Inc. ANIMEGH MANDAL SINSIL INTERNATIONALKOLKATA09331043269

2012 This document may not be reproduced or distributed in any form without the written permission of Enwave Optronics, Inc.

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2012 This document may not be reproduced or distributed in any form without the written permission of Enwave Optronics, Inc.

The Raman effect was theoretically predicted by Smekal in 1923The first experimental observation was made by Sir C. V. Raman in 1928C. V. Raman won the Nobel Prize in Physics in 1930 for this discoveryWavelength Shifted Light Scattering from MatterLess than one in a millions of photons scattered off matter will experience color (wavelength) changes reflecting the molecular structure of the substances.

1. The Science & Technology of Raman Spectroscopy

2. Applications of Raman spectroscopy

3. Enwaves Raman Instrument Products

2012 This document may not be reproduced or distributed in any form without the written permission of Enwave Optronics, Inc.

*Outline

2012 This document may not be reproduced or distributed in any form without the written permission of Enwave Optronics, Inc.

2012 This document may not be reproduced or distributed in any form without the written permission of Enwave Optronics, Inc. *The Science and technology of Raman Spectroscopy

2012 This document may not be reproduced or distributed in any form without the written permission of Enwave Optronics, Inc.

Raman spectroscopy is a vibrational spectroscopy for molecular analysis- Raman and IR are the most common vibrational spectroscopies for assessing molecular species and symmetry information- Raman spectroscopy is based on inelastic (energy changing) scattering of a monochromatic excitation source- Usual spectral range of interest: 200 - 4000 cm1- Raman has complementary selection rules to IR spectroscopy- Some vibrational modes are both IR and Raman activeGreat for many real-world sample analysis- Minimal sample preparation (gas, liquid, solid)- Compatible with wet samples and normal ambient condition- The worst challenge(interference) usually comes from sample fluorescenceOverview: Raman Spectroscopy

Raman spectroscopy is becoming one of the most attractive techniques and a standard equipment in laboratories

Raman provide information for easy fingerprinting identification of substances and is a valuable qualitative/quantitative tool in many areas: Pharmaceutical industryPetrochemical, industrial chemical, polymersMaterial scienceSemiconductors, photovoltaic materialsLife science (human, animal and plants : biology, biochemistry, biophysics, virus, tumor...)Geology and mineralogy, gemologyForensics sciencesArt, archeology, paints, pigment, stained-glass windows, earthenware, potteries, inks... The technique has become friendly of use and the costs are now accessible to all laboratories/industries Raman Technology Today

* Light-Matter Interaction and Optical Spectroscopy as Analytical Tools Transmission Part or whole energy pass through the substance

ReflectionBounce back from the surface of the substance

Absorption Part or the whole energy can be absorbed into the substance system (NIR, Mid IR, UV/Visible spectroscopy). Energy can be released from substances after absorption as re-emitted lights (Fluorescence/Emission spectroscopy) Scattering Light can be scattered off matter in all directions with or without energy changes (Raman spectroscopy)When Light Meets Matter, Light can experience:and gain useful information about matter

A modern Raman instrument is made up of 4 basic components:

Excitation source: An intense monochromatic light source is ideal for Raman spectroscopy. A frequency stabilized narrow line width laser is the best choice

Sampling probe with Rayleigh rejection filter

Spectrograph separates the light with different frequencies and detector that measures the energy, or intensity, of each component frequency. Computer System with software for instrument control, data acquisition, data processing and analysis. Components of Raman Spectroscopy Instrument

* Components in a Dispersive Raman Instrumentation

*Peak intensity yields sample concentrationPeak positions yields sample compositionCalibration set of sample components used for chemical analysis for real-time Chemometrics modeling and predictions Information from Raman Spectroscopy

Choose appropriate laser excitation to decrease fluorescence interference Fluorescence as a Raman Interference2012 This document may not be reproduced or distributed in any form without the written permission of Enwave Optronics, Inc.

2012 This document may not be reproduced or distributed in any form without the written permission of Enwave Optronics, Inc.

Sufficient spectral resolution and coverage (Spatial resolution)

Spectral coverage for the interested range Separate spectral peaks narrower than the narrowest anticipated spectral features of sample

Signal to Noise ratio (S/N)

Adequate S/N to provide high quality of Raman spectrum for analysis

Repeatability

Important factors for Raman Spectra

Comparison of Vibrational Spectroscopy Techniques

RamanMid IRNear IRPhenomenonScatteringAbsorptionAbsorptionInformationFundamental Vibrations (Fingerprint Detail) Sharp, well-defined peaksFundamental Vibrations (Fingerprint Detail) Sharp, well-defined peaksOvertone/combinationBroader, overlappingMore difficult to interpretRegions100-4000 cm-14000 to 200 cm-112800 ~ 4000 cm-1Type of Sample AnalyzedOrganics and InorganicsOrganics and InorganicsOrganics mainlyContaining C-H, N-H, O-HSampling IssuesSample PreparationNone, no needOften KBr Pellet or ATR(compression)NoneSample StateSolid, Liquid, slurry, paste, gel, suspension, etc.

Gases..very limitedSolids and liquidsrequiring varying degrees of samples preparation.Gasesvery goodSolid, Liquid, slurry, paste, gel, suspension, etc.

Gases..very limitedGlass VialsMinimal problem with GlassInterference from GlassMinimal problem with GlassWaterNo problem with WaterInterference from WaterNo problem with Water

Remote SamplingOver hundreds of metersVery limitedOver hundreds of meters

* The Applications of Raman Spectroscopy

*Targeted MarketsEnwave Optronics, Inc. (Confidential)

Enwave Optronics, Inc. (Confidential)

*Art and museum---- Pigment analysis ; Art Authentication; Archaeometric analysis of ancient pottery; Art restoration

Biology & Biomedical----- Dermatological Research; Human skin; SERS analysis of single living lymphocytes; wheat grain kernels; Teeth; Studies of transdermal drug transfer; Cancer identification (oral, skin, breast, cervical, colorectal); Studies of bone (composition, mechanics, and disease state); Soft dental tissue; Intravascular atherosclerosis; Studies of the eye (lens, fluids)..

BioTech---- Analysis of a Batch Fermentation Process

Carbon materials------ Carbon nanotube; Diamond; DLC; Graphite; Graphene; Study the effect of boron on the mechanical properties of diamond Examples of Raman ApplicationsEnwave Optronics, Inc. (Confidential)

Enwave Optronics, Inc. (Confidential)

*Catalysis---- In Situ Characterisation of Heterogeneous Catalytic Reactions; Investigation of Surface-Catalyzed NO Decomposition

Chemical-----The Formation of Phosphorus Trichloride from Phosphorus and Chlorine; The Production of Methyl Chlorosilanes ; Monitoring the Rutile/Anatase Ratio in TiO2 Production ; Headspace Gas Analysis ; Monitoring CaCO3 Polymorph Formation in the Presence of Polymeric Additives

Environmental and Agrochemical ---- Fertilizers; Peticides; Water Contamination

Raman Applications (Cont.)Enwave Optronics, Inc. (Confidential)

Enwave Optronics, Inc. (Confidential)

*Forensic ----- Ink; Forged document investigation; Identification of controlled drugs and narcotics; Explosive materials; Paint chip sample; Identifying Drugs of Abuse ; Pigment analysis; Plastics or textiles to commercial products

Food/ Nutrition Product----- Oils and Fats; Sugars; Sweeteners; Vitamins and Minerals; Beverages; Alcohol

Geosciences / Gemology / Mineralogy---- Geological materials analysis; Jade; Heavy mineral sands; Sandstone; Chemical Treatment Detection; Gem / Pearl / Coral Authentication & Identification; ..

Polymer----- Plastic recycling; Polymorph.. Raman Applications (Cont.)Enwave Optronics, Inc. (Confidential)

Enwave Optronics, Inc. (Confidential)

*Pharmaceuticals----- Polymorph in Pharmaceuticals; Incoming raw material ID; active pharmaceutical ingredients and excipients in a tablet; Final Product Testing..

Petrochemical

Reaction monitoring and process control---- Microwave Assisted Synthesis; Polymorph Characterization .

Recycling----- Plastic

Semiconductor----- Solar cell; Integrated Circuits; silicon stress in microelectronics

SERS Raman Applications (Cont.)Enwave Optronics, Inc. (Confidential)

Enwave Optronics, Inc. (Confidential)

Mixture in PFA YubeEnwave Optronics, Inc. (Confidential)

Enwave Optronics, Inc. (Confidential)

H2O2

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