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Page 1: Environmental Lab Catalogue
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ENVIRONMENTAL ENGINEERING LAB CATALOGUE
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Sarhad Univeristy of Science and Information Technology Peshawar
Page 2: Environmental Lab Catalogue

Page 1

Sarhad University of science and information technology

Lab

Engineering

Manual for

Environmental

Page 3: Environmental Lab Catalogue

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Sarhad University of science and information technology

Lab Manual for Environmental Engineering

S. No List of Practical’s Page No

01 To find turbidity of water by Nephelometric Method

ASTM Designation: D 1889 – 00

02

02 Standard Test Methods for pH of Water

ASTM Designation: D 1293 – 99

03

03 To Determine the Total Solids, Dissolved Solids, Suspended Solids

in a given sample of Water

ASTM Designation: D 1889 – 00

04

04 To find Hardness of water by standard EDTA method

ASTM Designation: D 1126 – 02

06

05 To find Alkalinity of a Water Sample by Indicator method

ASTM Designation: D 1067 – 02

07

06 To Determine Chloride Content in given water Sample by Argentic

Method

ASTM Designation: D 4458 – 94

08

07 To determine the optimum dosage for the turbid water by Jar test

ASTM Designation: D 2035-08

09

08 Estimation of chemical oxygen demand

ASTM Designation: D 1126 – 02

10

09 To determine the Biochemical Oxygen Demand of a given sample

ASTM Designation: D 4458 – 94

11

10 To Determine the Dissolved oxygen in a sample by Azide

Modification method

ASTM Designation: D 2035-08

13

11 To Determine Sulphide in a given Waste Water Sample by

Iodometeric method

ASTM Designation: D 1293 – 99

14

Page 4: Environmental Lab Catalogue

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Sarhad University of science and information technology

Standard Test method to find Turbidity of water by Nephelometric Method

ASTM Designation: D 1889 – 00 Theory:

Turbidity is caused in natural waters by finally divided suspended particles of clay, silt, sand

or by some organic materials, and by microscopic organisms. It is usually expressed in mg/l or

ppm, and may be determined by optical observations. The standard unit of turbidity is that

turbidity which is produced by mixing 1 mg of finally divided silica SiO2(called Fuller’s earth)

in one liter of distilled water which is equivalent to NTU. Turbid meters are generally used to

measure the turbidity of the given water samples. A turbid meter generally works on the

principle of measuring the interference caused by the water sample to the passage of light rays.

Apparatus:

Turbidity meter, Cuvette, Samples, Cleaning Solution

Standard:

WHO suggests a guide line value of 5 NTU.

Procedure:

i. Turn the meter on by pressing the ON/OFF key.

ii. The meter will carry out a self-test displaying full set of figures. After the test,

the LCD will change to the measurement mode.

iii. When the LCD displays ''-----'' the meter is ready to measure.

iv. Fill a clean cuvette up to one quarter inch (0.5cm) from its rim with

thoroughly agitated sample.

v. Allow sufficient time for bubbles to escape before securing the cap

vi. The cuvette must be completely free of finger prints and other oil or dirt.

vii. Place the cuvette into the cell and check that the notch on the cap is positioned

securely into the groove.

viii. The mark on the cuvette cap should point towards the LCD.

ix. Press the READ key and the LCD will display a blinking SIP (sampling in

process), turbidity value will appear after approximately 25 seconds.

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Sarhad University of science and information technology

Precautions:

Each time using tighten cap to same degree.

All glass ware use to contain samples should be washed with cleansing

solution or turbidity free water.

Observations & Calculation

Volume of Sample = a = -------- ml,

Volume of added distilled water = b = -------- ml

Dilution factor = (a + b)/a = --------

Turbidity of the sample = Dilution factor * NTU = -------- NTU

Sample # 01; Water Turbidity = -------- NTU

Sample # 02; Water Turbidity = -------- NTU

Sample # 03; Water Turbidity = -------- NTU

Page 6: Environmental Lab Catalogue

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Sarhad University of science and information technology

Standard Test Methods for pH of Water

ASTM Designation: D 1293 – 99

Theory:

The pH value of water or a solution is defined as the log of reciprocal of hydrogen ions

present in that water; i.e.

pH = log10 1/H+

Pure water is in fact a balanced combination of positively charged hydrogen ions (H+) and

negatively charged hydroxyl ions (OH-) both ions being equal. Moreover the product of H+

and OH-has been found to be 10-14 moles/liter. Hence in pure or a neutral water the quantum

of H+ and OH- will each be equal to√10-14 = 10-7 moles/litre.The pH value of such a pure

water will be equal to log10 1/10-7 = log10 107 = 7.The pH value of water indicates the acidity

or the alkalinity of water. The maximum acidity will at 0 value of pH and the maximum

alkalinity will be at pH value of 14.The pH value of raw water infact must be taken into

account while deciding the various treatment processes like coagulation Disinfection, water

softening etc. The pH value also becomes important in corrosion control since lower pH

values may cause tuberculation and corrosion of the pipes and treatment tanks. Higher pH

values may on the other hand produce incrustation, sediment deposit, difficulty in

chlorination.

Apparatus and Chemicals:

Buffer tables (pH4, pH7), Standard Solution, Probe pH meter stand, beaker

and Calorimetric paper as well as water sample.

Standards:

WHO suggested a guideline value of (6.5 to 8.5) pH of water

Page 7: Environmental Lab Catalogue

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Procedures:

Colorimetric Method:

i. Dip the Colorimetric paper in the water sample

ii. Compare the color of the paper with the color from the table and note the pH

of water against the color. This is pH of the sample

Electrometric Method

i. Connect pH electrode and temperature probe to the meter and switch it ON.

ii. Remove the protective cap; rinse the tips of pH electrode and temperature

probe with some pH 7.01 solution. Immerse them into a pH 7.01 buffer

solution, stir gently and wait a couple of minutes for stable reading.

a. Note: The electrode should be submerged approximately 4 cm (11/2'') into

the solution and temperature probe should be located as close as possible

to the electrode.

iii. Press the ºC key to display the temperature of the buffer (e.g. 20ºC).

iv. Press the pH key to read pH values. Stir gently and wait for couple of minutes.

pH values at various temperatures

Temperature pH values

ºC 4.01 7.01 10.01

0 4.01 7.13 10.32

5 4.00 7.10 10.24

10 4.00 7.07 10.18

15 4.00 7.04 10.12

20 4.00 7.03 10.06

25 4.01 7.01 10.01

30 4.02 7.00 9.96

35 4.03 6.99 9.92

40 4.04 6.98 9.88

45 4.05 6.98 9.85

Page 8: Environmental Lab Catalogue

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Sarhad University of science and information technology

Standard Test method to Determine the Total Solids, Dissolve Solids and

Suspended Solids in a given sample of Water

ASTM D5907 – 10 Solids:

Solids are defined as matter that remains as residue upon evaporation & drying at 103 20

Total Dissolved solids:

The total dissolved solids consist of minerals. This test provides a check on detailed analysis

in developing a potential source for public water supply i.e. if we want to develop a control

take off for the public water supply

Importance:

Determination of total solids in sewage is very much important as this sewage has to be

disposed off into the water body so if the total solids are more than the permissible limits by

NEQS then it will cause obstruction of sunlight and hence will be dangerous for the aquatic

life. High concentrations of dissolved solids in water when used in boilers may lead to boiler

troubles like priming and foaming.

Total Solids Dissolved

Solids

Volatile Solids

Fixed Solids

Total Solids Suspended Solids

Settleable Solids

Non Settleable Solids

Page 9: Environmental Lab Catalogue

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Sarhad University of science and information technology

Suspended Solids:

Apparatus:

Filter paper, beaker, desiccators, oven, balance, filtration assembly, vacuum

pump as well as conical flask.

Standard:

WHO guideline value = 1000 mg/l as dissolved solids

Procedure:

i. Weight the filter paper and let it be W1

ii. Put the filter paper in the filtration apparatus and pass some distill water by

applying vacuum to set the filter paper.

iii. Pass the measured amount of sample through filter paper

iv. Place the filter paper in an oven for one hour.

v. Weight the filter paper and let its weight be W2

Suspended solids (mg/l) = (W2 – W1)*1000/Volume of Sample

Total Dissolved Solids:

Apparatus:

China dish, Balance, Desiccators

Standards:

NEQS National Environmental Quality Standards values = 3500mg/l

Procedure:

i. Take clean china dish weight it in accurate balance let its weight be W1

ii. Take some of the sample from china dish and do steam bathing for one hour

iii. After that place the china dish in oven at 103 20

iv. When it is completely dried put it in desiccators

v. Reweigh the china dish along with the solid residual, let it be W2

Total Dissolved Solids (mg/l) = (W2 – W1) *1000/Volume of sample

Total Solids (mg/l) = Suspended Solids + Total Dissolved Solids

Page 10: Environmental Lab Catalogue

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Sarhad University of science and information technology

Standard Test method to find Hardness of water by standard EDTA

method

ASTM Designation: D 1126 – 02

Scope and significance:

Hardness salts in water, notably calcium and magnesium, are the primary cause of tube and

pipe scaling, which frequently causes failures and loss of process efficiency due to clogging,

loss of heat transfer. This test method covers the determination of hardness in water by titration.

This test method is applicable to waters that are clear in appearance and free of chemicals that

will complex calcium or magnesium. The lower detection limit of this test method is

approximately 2 to 5 mg/L as CaCO3; the upper limit can be extended to all concentrations by

sample dilution. It is possible to differentiate between hardness due to calcium ions and that

due to magnesium ions by this test method.

Apparatus and Chemicals used:

Conical Flask, funnel, sand, burette, beaker, Buffer solution, Eriochrome Black

– tea, EDTA solution of 0.02 Normality.

Standard:

Who guideline value of hardness is 500 mg/liter as CaCO3

EU guidelines = 250 mg/liter as CaCo3

Procedure:

i. Take 50ml sample into Erlenmeyer flask.

ii. Add 3 to 5 ml buffer solution to flask.

iii. Add few drops of Eriochrome black indicator.

iv. Mix solution, color will be pink.

v. Fill the burette with 0.02N EDTA solution, and note the reading (initial

reading).

vi. Start titration.

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vii. When the color changes to blue, stop titration and note the reading (final

reading)

viii. Calculate the hardness by using the following formula

Hardness= (F.R- I.R) x N x 50 x 1000 = mg/l as CaCo3

Volume of sample in ml

Type of Water Hardness (mg/lit)

Soft Water 0-75

Moderately Hard Water 75-150

Hard Water 150-300

Very Hard Water Above 300

Page 12: Environmental Lab Catalogue

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Sarhad University of science and information technology

Standard Test method to find the Alkalinity of a water sample by Indicator

Method

ASTM Designation: D 1067 – 02

Theory:

Alkalinity of water is a measure of its capacity to neutralize acids i.e. to absorb hydrogen ions

without significant pH change. It is caused by the presence of hydroxides (OH-), carbonates

(CO--) and bicarbonates (HCO3-).A negligible amount of negative alkalinity is also caused by

hydrogen ions (H+) which is very small and hence ignored. Presence of carbonate and

bicarbonate in water is infact an interchangeable process and depends upon the pH value of

water. At lower value of pH the carbonates change to bicarbonates so much so that at pH less

than 8.3 only bicarbonates are found to occur. Since all the carbonates are converted into

bicarbonates by the action of CO2 and H2O as indicated below

pH< 8.3

CO2 + H2O + CaCO3 Ca(HCO3)2

Even bicarbonates cease to exist in water if its pH falls below 4.5 since they are all converted

into carbonic acid.

Significance and Use:

Acidity and alkalinity measurements are used to assist in establishing levels of

chemical treatment to control scale, corrosion, and other adverse chemical

equilibrium.

Levels of acidity or alkalinity are critical in establishing Solubility of some metals,

toxicity of some metals, and the buffering capacity of some waters.

Standard:

WHO suggested a guide line value for the alkalinity (mg/l as CaCo3)

i. Low Alkalinity < 50

ii. Medium Alkalinity: 50 - 250

iii. High Alkalinity > 250

Page 13: Environmental Lab Catalogue

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Apparatus and Chemicals used:

Stand, burette, pipette, funnel, conical flask, beaker.

Phenolphthalein indicator solution, brome cresol green + methyl red solution,

standard solution (H2SO4) of 0.02N.

Procedure:

i. Take 50 ml of water sample in a flask.

ii. Add six drops of phenolphthalein indicator in the sample.

iii. Fill the burette with standard H2SO4 solution and note the reading (initial

reading)

iv. Start titration

v. When the color of sample changes, stop titration and note the reading of

burette (final reading)

vi. Calculate the phenolphthalein alkalinity by using following formula

P- Alkalinity = (F.R – I.R) x 1000/50

vii. Now add six drops of brome cresol green methyl red solution, which will turn

the color of sample to green.

viii. Note the initial reading of burette having standard H2SO4 solution.

ix. Start titration

x. When the color changes to grey, stop titration and note the final reading.

xi. Calculate the total alkalinity using the following formula

Total Alkalinity = (F.R – I.R) x 1000/50

Relationship table for Alkalinity:

Result of Titration Hydroxide Carbonate Bicarbonate

P = 0 Nil Nil T

P > T/2 2P – T 2(T-P) Nil

P = T/2 Nil 2P Nil

P < T/2 Nil 2P T – 2P

P = T P Nil Nil

Where P = Phenolphthalein T = Total Alkalinity

Page 14: Environmental Lab Catalogue

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Sarhad University of science and information technology

Standard Test method to Determine Chloride Content in given water

Sample by Argentic Method

ASTM Designation: D 4458 – 94

Theory:

Chloride is always present in water in some amount. The excess presence of NaCl in water

indicates pollution of water due to sewage. The salt used in the preparation of food is

excreted by body thereby making sewage higher in Chlorides. Chlorides in reasonable

concentrations are not harmful to humans. At concentrations above 250mg/l they give a salty

taste to water which is objectionable to many people for this reason chlorides are generally

limited to 250mg/l in supplies intended for public use.

Importance:

This test indicates presence of Chlorides in the sample. If it comes out greater than

1000mg/liter then it indicates pollution of water by sewage and we must stop the supply.

Standards:

According to WHO Guidelines (1970)

i. Higher Desirable Limits = 200mg/liter as CaCo3

ii. Higher Desirable value = 250mg/liter (1984) as CaCo3

Apparatus and Chemicals:

Burette, Titration Flask, Water Sample Conical Flask, Graduated Cylinder,

Stand and pipette beaker, Potassium chromate indicator (K2CrO4, Standard

Silver Nitrate)

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Procedure:

Apparatus Assembly:

A burette fitted in stand with the beaker placed below the burette

i. Take 50 ml of sample

ii. Add 0.5 ml of Potassium Chromate to it ,it will become yellow

iii. Take Silver Nitrate, dissolve in distilled water and get 2.39 mg/liter solution of

water

iv. Take this Silver Nitrate solution in burette and note the initial reading

v. Titrate potassium chromate solution against silver nitrate solution till it

changes its color to pinkish yellow, note the final reading.

Chloride content (mg/l) = (Final Reading –Initial reading)*35.45*N*1000/50

Precautions:

i. Hands should be washed immediately as these solutions are harmful.

Page 16: Environmental Lab Catalogue

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Standard Test method to Determine the optimum dosage for the turbid

water by Jar test

ASTM Designation: D 2035-08 Theory:

Alum is the name given to the Aluminium Sulphate with its chemicals formula a

Al2(SO4)3.18H2O. The Alum when added to raw water reacts with bicarbonate alkalinities

which are generally present in raw water so as to form gelatinous precipitate (floc) of

aluminium hydroxide. This floc attracts pure suspended matter and collides present in raw

water, Thereby growing in size. The floc formation is assisted by slow mixing called

floatation. The flocs finally settle down to the bottom of the tank for being removed in the

sedimentation tank. The above process/technique is known as coagulation.

Importance:

Jar test is usually performed for the determination of dosage of coagulants

Apparatus:

Water sample, jars, spin block, Electric stirrer, Coagulants etc

Procedure:

Six jars arranged in the jar apparatus

i. Take six no of jars

ii. Fill them with water for which dosage of coagulant is to be determined

iii. Arrange them in jar apparatus

iv. Various amount of coagulant are then added to each of the jar

v. After reading the coagulant the driving unit is started

vi. It starts rotation of the pink block

vii. Allow the rotation for 30 minutes

viii. For 1 hour allow or leave the apparatus and then the formation of floc in each

jar is noted.

Coagulant Dosage:

i. For high turbidity: 3 to 5 gpg

ii. For lower turbidity: 0.1 to 1 gpg

Precautions:

i. Carefully adjust the apparatus

ii. As the apparatus work with electricity , so care should be taken

Page 17: Environmental Lab Catalogue

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Standard Test method for Estimation of chemical oxygen demand (COD)

ASTM Designation: D1252-06

Theory and principle:

These are some organic matters especially from industrial waste that is toxic to bacteria and it

is difficult to calculate bacteria as well. COD is calculated for these types of wastes

COD Definition:

Amount of oxygen required by the strong oxidizing agent to completely oxidize the organic

matter under acidic conditions. In BOD the organic matter is not completely oxidized

Biologically degradable organic matter is only oxidized on the other hand in COD both the

Biologically degradable and biologically inactive organic matter is oxidized as a result COD

is always greater than BOD

Advantages of COD test:

i. This test can be performed in short duration 3hrs while BOD takes 5 days

ii. If sufficient data is available on BOD and COD of particular sewage

accumulated ration of BOD and Cod can be determined

iii. The most readily oxidizing agent K2Cr2O7 can be used.

Importance:

COD shows the oxygen required to oxidize the organic as well as the in organic matter

present in the waste water. It very important to determine as its excess will cause more

oxygen consumption and is hazardous to aquatic life.

Apparatus and Chemicals:

Standard K2Cr2O7 solution 0.025N, H2SO4, Standard ferrous ammonium

sulphate solution 0.1N, Ferrous indicator solution, Mercuric Sulphate,

Sulphuric acid (required if the interference of nitrate is to be eliminated,

Reflux apparatus, COD apparatus

Page 18: Environmental Lab Catalogue

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Standards:

NEQS standard value is 150 mg/l

Procedure:

A fluxing flask with mercuric sulphate and K2Cr2O7 added to sewage sample

i. 0.4ml of mercuric sulphate is taken in fluxing flask appropriately. Then 29ml

of sewage sample and 10 ml of standard K2Cr2O7 is added to the above flask

along with several granular of glass beats to stop splitting of liquid during

boiling

ii. The flask is connected to the condenser 30ml of concentrated H2SO4

containing AgSO4 is added slowly through open end of condenser and it is

mixed thoroughly by swirling while adding the acid. The reflux mixture is

mixed thoroughly before heat is supplied if this is not done local heating

occurs in the bottom of the flask and the mixture may be blown out of the

condenser

iii. The mixture is heated for two hours; it is cooled and waved down the

condenser with distilled water. The mixture is diluted to about 150ml with

distilled water, cooled at room temperature, and excess of dichromate standard

ferrous ammonium sulphate using ferrous indicator.(2 drops)

iv. The end point is sharp color change from blue green to reddish brown.

v. The experiment is repeated and refluxed in the same manner without sample

(blank) consisting of 20ml of distilled water together with the reagent

Page 19: Environmental Lab Catalogue

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Sarhad University of science and information technology

Standard Test method to determine the Biochemical Oxygen Demand of a

given sample

ASTM Designation: WK 23808 Theory:

Biochemical oxygen demand is the amount of oxygen required for the microorganisms

present in waste water to convert the organic substance to stable compounds such as CO2 and

H2O

Organic substance + oxygen + Bacteria CO2 + H2O

Importance:

Biochemical oxygen demand is the amount of oxygen required for the microorganisms

present in waste water to convert the organic substance to stable compounds such as CO2 and

H2O.Its determination is very much important because it will give you the oxygen consumed

more BOD greater will be its effect on the aquatic life.

Apparatus and Chemicals:

BOD bottle, Burette, Pipette, Pipette filler, Graduated cylinder, Manganese

sulphate, alkali iodine acid, concentrated sulphuric acid, standard thiosulphate

and starch indicator.

Standards:

i. The BRCES (British Royal Commission Effluent Standard) allow a BOD of

20 mg/l in a treated sewage to be discharged in to any water body.

ii. BOD Value according to NEQS = 80 mg/l

Procedure:

BOD bottle fitted with stopper and pipette

i. Take two BOD bottles and half fill it with distilled water

ii. Add 3ml of waste water (polluted water ) to the BOD bottle with the help of

pipette

iii. Fill the tube with distilled water and fix the stopper on it.

iv. Put one of these tubes in incubator at 20°C for five days.

v. Add 2ml of MnSO4 to other tube with the help of pipette and shake it well (if

oxygen is present the color will be brown otherwise white)

vi. Add 2ml of concentrated H2SO4 and shake well which will give a color which

is in resemblance to mustard oil.

vii. Take 200ml from this solution in graduated cylinder and add 1ml of starch

indicator to it which will give a yellowish color

viii. Place the graduated cylinder below the burette containing the standard solution

of sodium thiosulphate and note the initial reading

ix. Find dissolved oxygen by subtracting the initial reading from final reading

x. After incubation of first tube the dissolved oxygen is found in similar way

xi. Find the BOD by using the formula

BOD (mg/l) = (Zero day DO – Five Days DO)*300 mg/l of sample

Page 20: Environmental Lab Catalogue

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BIOCHEMICAL OXYGEN DEMAND (BOD) DATA

Sample source: ------------------------

Raw sewage Bottle number (not lid #) ---------------------------

Sample volume (ml) --------------------------

Initial DO (mg/L) --------------------------

Final DO (mg/L) --------------------------

Final Effluent Bottle number

Sample Volume (ml) --------------------------

Initial DO (mg/L) --------------------------

Final DO (mg/L) -------------------------

Dilution Water Bottle number

Sample Volume (ml) --------------------------

Initial DO (mg/L) --------------------------

Final DO (mg/L) -------------------------

BOD5 Calculation:

When dilution water is not seeded

BOD = (D1 - D2)/P

When seeded water is used

BOD =( (D1-D2) – (B1-B2) f)/P

Where

D1 = DO of diluted sample immediately after preparation, mg/L

D2 = DO of diluted sample after 5 d incubation at 20°C, mg/L

P = Decimal Volumetric fraction of sample used

B1 = DO of seed control before incubation, mg/L

B2 = DO of seed control after incubation, mg/L

f = ratio of seed water in diluted sample to seed in seed control =

(% seed in diluted sample)/ (% seed in seed control)

Summary:

Raw sewage, Diln. 1 -------------------- --------------------

Raw sewage, Diln. 2 -------------------- ------------------- Average --------------

Final effluent Diln. 1 -------------------- --------------------

Final effluent Diln. 2 -------------------- -------------------- Average --------------

Page 21: Environmental Lab Catalogue

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Standard Test method to Determine the Dissolved oxygen in a sample by

Azide Modification method

ASTM D888 – 09

Theory:

Dissolved oxygen is essential for the maintenance of healthy lakes and rivers .the presence of

oxygen in water is a good sign. The lack of oxygen is a signal of severe pollution. Rivers

range from high to very low levels of dissolved oxygen. Physically dissolved oxygen is

influenced by water temperature and the volume of water moving down a river (discharge)

affect dissolved oxygen levels. Gases like oxygen dissolve more easily in cooler water than in

warmer water. In temperate areas rivers respond to changes in air temperature by cooling or

warming. River discharge is related to the climate of an area. during dry periods flow may be

severely reduced and air and water temperatures are often higher both of these factors tend to

reduce dissolve oxygen levels wet weather or melting snow increase flow with a resulting

greater mixing of atmospheric oxygen.

Apparatus:

Burette, Pipette, pipette filler, beaker, Dissolved oxygen Bottle

Reagents:

MnSO4, Alkali Iodide Azide reagent, H2SO4, Starch Indicator, Standard

Sodium Thiosulphate titrant (0.025N)

Procedure:

i. Take water sample in 300ml bottle

ii. Add 1ml MnSO4 solution followed by 1ml Alkali Iodide Azide solution

iii. Cap the bottle making sure no air is trapped inside and invert repeatedly to

fully mix. The presence of oxygen is indicated by the formation of brownish

orange precipitate and will form floc

iv. Allow the sample to stand until the precipitate settles halfway

v. Add 1ml concentrated H2SO4 cap it and invert repeatedly until the reagent and

precipitate have been dissolved. A clear yellow to brown orange color will

develop.

vi. Add 1ml starch indicator solution the sample will turn blue

vii. Titrate against standard Sodium thiosulphate solution 0.025N until the sample

changes from blue to colorless solution and note the burette reading

Page 22: Environmental Lab Catalogue

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Dissolved Oxygen Data:

Sample/Data Raw Sewage Final Effluent Tap Water Dil’n Water

Bottle#

Sample Volume

(mls)

Initial Burette

Reading

Final Burette

Reading

Net Titre (mls)

DO Conc (mg/l)

DO Conc

(Probe)

Temp(°C)

Saturation

Concentration

Page 23: Environmental Lab Catalogue

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Standard Test method to Determine Sulphide in a given Waste Water

Sample by Iodometeric method.

ASTM D4658 - 09 Theory:

Sulphide is poisonous by product of anaerobic decomposition of organic matter mostly in

waste water and industrial waste. Sulphide present in ground water especially in hot springs.

The threshold odor concentration of H2S in clear water is 0.025mg/l to 0.25mg/l. If it is

attached directly or indirectly causes serious corrosion of concrete sewers because it is

oxidized biologically to H2S on the pipe walls.

Importance:

Sulphide is poisonous by product of anaerobic decomposition of organic matter mostly in

waste water and industrial waste .It also cause serious corrosion of concrete sewers because it

is oxidized biologically to H2S on the pipe walls. That is why its determination is very

important.

Chemicals and Apparatus:

HCl 6N, Iodine Solution 0.025N,Na2S2O3 0.025N, starch Indicator, Beaker,

Pipette

Standard:

Standard value for sulphide in waste water is not more than 1mg/l

Procedure:

Apparatus Assembly:

i. A burette fitted in stand with the conical flask placed below the burette.

ii. Take 200 ml sample of water .Add some iodine solution .On disappearance of

iodine color add more iodine to remain the yellow color.

iii. Then add 2 ml 6N HCl. Take Na2S2O3 solution for titration and a few drops of

starch solution. At the end point until the blue color disappears note the

reading.

Sulphide mg/l = (A*B)-(C*D)*16*1000/ml of Sample

A = ml of Iodine solution

B = Normality of Iodine

C = ml of Na2S2O3 solution

D = Normality Na2S2O3 solution

Page 24: Environmental Lab Catalogue
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LAB INVENTORY
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Page 25: Environmental Lab Catalogue

S.No Category Type Sub Type Make Model Serial No Given No Issue Date Incharge Location NameLocation

NumberStatus

Status

Date

Status

Authority

1Non

ConsumedApparatus Digital Spectrophotometer _ 721 _ 18/7/2010 _ Serviceable 12/11/2014 CED

2Non

ConsumedApparatus Analytical Balance HAND DHV300A _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

3Non

ConsumedApparatus Electric stirrers CHINA JJ 1 _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

4Non

ConsumedApparatus Turbidity meter

HANNA

(Romania)Hi 93703 _ 18/7/2010 _ Serviceable 12/11/2014 CED

5Non

ConsumedApparatus Filtration assembly with Vacuum pump SPARMAX L 100432 _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

6Non

ConsumedApparatus IMhoff cone SAN DIN 12672 _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

8Non

ConsumedApparatus Water Distillation assembly _ 2001009423 _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

9Non

ConsumedApparatus Burettes with stands JAPSON SCES 047 _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

10Non

ConsumedApparatus Desiccators PAKISTANI _ _ 18/7/2010

Lab Incharge: Engr. Shahab

Ahmad _ Serviceable 12/11/2014 CED

13Non

ConsumedApparatus pH meter ADWA AD 8000 _ _ 18/7/2010

Lab Engr: Engr.Muhammad

Abdur Rahman_ Serviceable 12/11/2014 CED

14Non

ConsumedApparatus pH meter

HANNA

(Romania)HI 83141 _ _ 18/7/2010 Lab attendent: Abu bakar _ Serviceable 12/11/2014 CED

15Non

ConsumedApparatus Pippett IWAKI PUIREX _ _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

16Non

ConsumedApparatus Hot air oven

JIANGSU

ZHENGJI GRX-28A _ _ 18/7/2010 _ Non-Serviceable 12/11/2014 CED

17Non

ConsumedApparatus BOD bottels WHEATON _ _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

18Non

ConsumedApparatus Funnels IWAKI PUIREX _ _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

19Non

ConsumedApparatus Barkers IWAKI PUIREX _ _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

20 Consumed Chemical Sodium ozaide ANALAR _ _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

21 Consumed Chemical Nitric Acid(conc.) (HNO3) ANALAR _ _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

22 Consumed Chemical Sulfuric Acid (H2SO4)RIEDEL-

DEHAEN_ _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

23 Consumed Chemical Ammonia solution (NH3) ANALAR _ _ _ 18/7/2010 _ Serviceable 12/11/2014 CED

Chemical Phenolphthalein (C20 H14 O4)

Chemical

RIGK

ANALAR

Sarhad University of Science and Information Technology, Peshawar

Inventory List

Environmental Engineering Lab

Civil Engineering Department

Consumed

MERCK

GERMANY

Sodium carbonate (Na2CO3)

Starch soluble (C6H10 O5)n

Aluminum sulfate (Al2 O12 S3. 18H2O)

Consumed

MERCK

GERMANY

Consumed Chemical ANALAR

Chemical

Consumed

Consumed

Chemical

Consumed Chemical Silver nitrate (AgNo3) 25gm*8MERCK

GERMANY

Consumed Chemical

Bromophenol blue

ANALARErichrome black - T

_

_

_

_

_

_

_

_

_

_

_

_

_

_

_

_

_

_

_

_

_ 18/7/2010

18/7/2010

18/7/2010

18/7/2010

Serviceable

Serviceable

Serviceable

serviceable

Serviceable

_

_

_

_

_

_ Serviceable

_ Serviceable 12/11/2014

12/11/2014

12/11/2014

12/11/2014

12/11/2014

12/11/2014

12/11/2014

CED18/7/2010

18/7/2010

18/7/2010

CED

CED

CED

CED

CED

CED30

24

25

26

27

28

29

Environmental

Engineering Lab

Page 26: Environmental Lab Catalogue

_

3/5/2014

Consumed

Chemical

Chemical

3/5/2014

MERCK

GERMANY31

32

33

Ammonium chloride (NH4 CL)

Potassium chromate ( K2 Cr O)

Methyl orangeChemical

Consumed Chemical

Consumed

ANALAR

MERCK

GERMANY

Methanol (CH4O)MERCK

GERMANY

_

_

_

_

_

_

_

_

_ 18/7/2010

18/7/2010

18/7/2010

18/7/2010

18/7/2010

_ _ _

Chemical Ethanol MERCK

GERMANY_ _ _

Serviceable

_

Serviceable

Serviceable

12/11/2014

12/11/2014

_ Serviceable

_ 12/11/2014

12/11/2014 CED

CED

CED

CED

CED

Consumed

Consumed

3/5/2014

_ Serviceable 12/11/2014

_

_

Consumed Chemical Acetic acid (C2 H4 O2)RIEDEL-

DEHAEN_

Consumed

Consumed

Consumed

Consumed

_ _ 18/7/2010

_

_

_

_

_

_

Serviceable 12/11/2014 CED

_ 12/11/2014 CEDServiceable

_

_

_

_

_

_

_

_

_

3/5/2014

Chemical

Chemical

Chemical

Chemical

Potassium iodide (KI)

Sodium iodide (Na l)

Silica gel

Sodium hydrogen carbonate (NaHCO3)

MERCK

GERMANY

ANALAR

MERCK

GERMANY

MERCK

GERMANY

_

_

_

_ _

18/7/2010

18/7/2010

18/7/2010

18/7/2010

_

_

_

_

_

_

Serviceable

Serviceable

_

_

_

_

_

_

_

12/11/2014

12/11/2014

12/11/2014Serviceable

_

CED

CED

CED

_

_

Consumed

41

42

40

43

44

45

Consumed

Consumed

Consumed

Consumed

Consumed

Consumed

Magnesium sulfate – heptahydrat- (Mg SO4

7H2O)

Chemical

Chemical

Chemical

Chemical

Chemical

Chemical

Bromophenol blue

Pnenol cryst extra pure (C6 H6 O)

Tri ethanolamine (C6 H15 NO3)

Aluminum sulfate – 18 hydrate (AL2

(SO4)3.18H2O)

Sodium hydroxide pellets (Na OH)

Mercury (11) nitrate Hg (No3)2. H2O

MERCK

GERMANY

MERCK

GERMANY

MERCK

GERMANY

MERCK

GERMANY

MERCK

GERMANY

GPR

_

_

_

_

_

_

_

_

_

_

_

_

_

_

18/7/2010

18/7/2010

18/7/2010

18/7/2010

18/7/2010

18/7/2010

52

Non

Consumed

Non

Consumed

Electrical

appliances

Electrical

appliances

Stools for Students

Fans

Energy Savers

Non

ConsumedFurniture

_ Serviceable

_ _MERCK

GERMANY_

_

_

_

_

_

_

_

_

_

18/7/2010

12/11/2014

_

Serviceable

Serviceable

Serviceable

Serviceable

Serviceable

Serviceable

Serviceable

_ CED

CED

CED

CED

CED

Serviceable

CED

CED

CED

12/11/2014

34

35

Tables for aparatus

Staff table

12/11/2014

12/11/2014

12/11/2014

12/11/2014

12/11/2014

Non

Consumed

Furniture

Furniture

36

37

38

39

46

47 Chemical

50

51

12/11/2014

12/11/2014

12/11/2014

12/11/2014

Serviceable48

49

Non

Consumed

_ 3/5/2014 Serviceable

Serviceable

Serviceable

Serviceable_ _ 3/5/2014

_

_

_54

Non

Consumed

Non

Consumed

Electrical

appliances

Ceramics

Exchaust fan

Wash Basin

53 CED

CED3/5/2014

_

_

CED

CED

CED

CED12/11/2014

Furniture White Board

Serviceable

12/11/2014

12/11/2014_

_

_

_

_

12/11/2014 CED55

Non

Consumed_ _ _ _ 3/5/2014