enhancement by hydrogel polymers of salt resistance in poplar · enhancement by hydrogel polymers...
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Enhancement by Enhancement by HydrogelHydrogel Polymers of Salt Polymers of Salt Resistance in PoplarResistance in Poplar
Shaoliang ChenShaoliang Chen
College of Biological Sciences and Technology, Beijing Forestry College of Biological Sciences and Technology, Beijing Forestry University, University, Beijing 100083, PR ChinaBeijing 100083, PR China
InstitutInstitut ffüürr ForstbotanikForstbotanik, , GeorgGeorg--AugustAugust--UniversitUniversitäätt GGööttingenttingen, , BBüüsgenwgsgenwg 2, 2, 37077, 37077, GGööttingenttingen, Germany, Germany
Beijing, Oct 29th, 2008Beijing, Oct 29th, 2008
The 23rd Session of the International Poplar Commission
Soil salinization is severe Soil salinization is severe in China and salinein China and saline--alkali alkali land is approximately 30 land is approximately 30 million hectares, which is million hectares, which is mainly distributed in the mainly distributed in the north of Yangtze River. north of Yangtze River.
China ThreeChina Three--North Plantation ProjectNorth Plantation Project
Soil Soil salinizationsalinization
StockosorbStockosorb polymerpolymer
Stockhausen GmbH, Stockhausen GmbH, KrefeldKrefeld, Germany, Germany
HydrogelHydrogel increased salinity tolerance of increased salinity tolerance of P. P. euphraticaeuphratica ⎯⎯A 2A 2--year experiment in year experiment in GGööttingenttingen Univ. GermanyUniv. Germany
HydrogelHydrogel amendment:amendment:
Potassium mine refuse in Potassium mine refuse in BischofferodeBischofferode(Germany)/abundant with (Germany)/abundant with NaClNaCl
0.6% (0.6% (v/vv/v) ) StockosorbStockosorb K410, K410, which is a highly which is a highly crosslinkedcrosslinkedpolyacrylamidepolyacrylamide with about with about 40% of the amide group 40% of the amide group hydrolysedhydrolysed to carboxylic to carboxylic groups.groups. + 2.7-fold biomass
Effects of Effects of hydrogelhydrogel on on cellular ion relations (by cellular ion relations (by EDAX): EDAX):
restricted restricted apoplasticapoplastic NaNa+ + in in young and old rootsyoung and old rootslimited limited apoplasticapoplastic and and cytoplasticcytoplastic ClCl-- in old roots in old roots increased increased ClCl--compartmentationcompartmentation in in cortical vacuolescortical vacuolesenhanced uptake of Caenhanced uptake of Ca2+2+
HydrogelHydrogel--covered rootscovered roots
Chen S, et al. 2004. Chen S, et al. 2004. Trees 18, 175Trees 18, 175--183.183.
ObjectivesObjectives
Is the polymer working in salt soil in China?Is the polymer working in salt soil in China?
Is the polymer able to enhance salinity Is the polymer able to enhance salinity tolerance of salttolerance of salt--sensitive poplars?sensitive poplars?
Experimental treatmentsExperimental treatments
Two species: Two species: P. euphratica, P. popularisP. euphratica, P. popularis
Control Control ±± hydrogelhydrogelNaClNaCl −− hydrogelhydrogelNaCl + hydrogelNaCl + hydrogel
Hydrogel:Hydrogel: StocksorbStocksorb 500 XL, 0.5% (v/v)500 XL, 0.5% (v/v)NaCl :NaCl : Addition of 1L 250 mM NaCl to 10 L Addition of 1L 250 mM NaCl to 10 L
soil/per pot, reaching a final concentration of soil/per pot, reaching a final concentration of ca. 55 mM in soil solutionca. 55 mM in soil solution
Plant response to salinityPlant response to salinity
处理处理TreatmentTreatment
胡杨胡杨PP..euphraticaeuphratica
群众杨群众杨PP..popularispopularis
ControlControl
NaClNaCl
NaCl + hydrogelNaCl + hydrogel
NormalNormal
NormalNormal
NormalNormal
NormalNormal
Injured/25dInjured/25d
Injured/45dInjured/45d
Salt injurySalt injury ⎯⎯ P. P. popularispopularis
NaCl + hydrogel
NaCl − hydrogel
Unit transpiration rateUnit transpiration rate
0
2
4
6
8
12 18 25
蒸腾
速率
(TR
N)/m
mol
.m-2.s
-1
Tran
spira
tion
rate
/mm
ol.m-2
.s-1
controlNaClNaCl and hydrogel
0
2
4
6
8
12 18 25
controlNaClNaCl and hydrogel
胡杨 群众杨P.euphratica P.popularis
Time/d Time/d
0
100
200
300
12 18 25
气孔
导度
(GS)
/mm
ol.m-2
.s-1
Stom
atal
con
duct
ance
/mm
ol.m-2
.s-1 control
NaClNaCl and hydrogel
0
100
200
300
12 18 25
controlNaClNaCl and hydrogel
Time/d Time/d
胡杨 群众杨P.euphratica P.popularis
StomatalStomatal conductanceconductance
0
2
4
6
8
10
12
12 18 25
time/d
净光
合速
率(P
n)/μ
mol
.m-2
.s-1
Net
pho
tosy
nthe
tic ra
te/μ
mol
.m-2
.s-1 control
NaClNaCl and hydrogel
0
2
4
6
8
10
12
12 18 25time/d
controlNaClNaCl and hydrogel
胡杨 群众杨P.euphratica P.popularis
Net photosynthetic rateNet photosynthetic rate
Leaf ion concentrations Leaf ion concentrations ((mmolmmol gg--11DW)DW)
树种species
处理Treatment Cl- Na+ K+ Ca2+ Mg2+
胡杨
P. euphratica
群众杨
P. popularis
Control
NaCl
NaCl + hydrogel
Control
NaCl
NaCl + hydrogel
0.22c
0.52a
0.38b
0.20c
1.16a
0.64b
0.05c
0.23a
0.16b
0.03c
0.48a
0.27b
0.53a
0.42b
0.56a
0.38b
0.41b
0.50a
0.09a
0.08a
0.07a
0.10a
0.10a
0.10a
0.17a
0.16a
0.15a
0.18a
0.18a
0.19a
Soil ion concentrations (Soil ion concentrations (mMmM))
处理Treatment Cl- Na+ K+ Ca2+ Mg2+
Control
NaCl
NaCl and hydrogel:⎯ Soil⎯ Hydrogel granules
Hydrogel products
14.2d
64.1a
39.7b29.9c
0.2e
7.0c
48.9a
36.6b2.7d
1.7e
4.4b
1.0c
4.5b0.8c
6.8a
8.3a
7.9a
7.8a1.5b
0.1c
12.3a
10.5a
12.3a2.1b
0.3c
Root aggregation in Root aggregation in hydrogelhydrogel granulesgranules ⎯⎯ P. P. popularispopularis
NaClNaCl + + hydrogelhydrogel
NaClNaCl −− hydrogelhydrogel
Root aggregation in Root aggregation in hydrogelhydrogel granulesgranules ⎯⎯ P. P. euphraticaeuphratica
NaClNaCl −− hydrogelhydrogel
NaClNaCl + + hydrogelhydrogel
RootRoot--shoot salt transport regulationshoot salt transport regulation
Is the polymer working in salt Is the polymer working in salt soil in China?soil in China?
⎯⎯Yes.Yes.Is the polymer able to enhance Is the polymer able to enhance salinity tolerance of saltsalinity tolerance of salt--sensitive poplars?sensitive poplars?
⎯⎯Yes.Yes.
HydrogelHydrogel effects:effects:Salt buffering capacitySalt buffering capacityNutrient source, e.g. KNutrient source, e.g. K++
Na+, Cl-
AcknowledgementsAcknowledgementsInstitut fInstitut füür Forstbotanik der r Forstbotanik der
UniversitUniversitäätt GGööttingenttingen
Prof. Dr. Prof. Dr. AloysAloys HHüüttermannttermannProf. Dr. Andrea PolleProf. Dr. Andrea PolleDr. Dr. EberhardEberhard FritzFritzMs. Karin LangeMs. Karin LangeMr. Mr. MoitabaMoitaba ZommorodiZommorodiMr. Mr. KonradKonrad WehrWehrMs. Christine Ms. Christine KettnerKettner
Beijing Forestry UniversityBeijing Forestry University
Prof. Shasheng WangProf. Shasheng WangMs. Ms. JinkeJinke Li Li Mr. Mr. JieJie ShaoShaoMr. Yong ShiMr. Yong ShiMr. Mr. JianJian SunSunDr. Dr. RuigangRuigang WangWang
The Hebrew University of The Hebrew University of JerusalemJerusalem
Prof. Dr. Prof. Dr. ArieArie Altman Altman
Structure of Structure of acrylamideacrylamide-- potassium potassium acrylateacrylate crosslinkedcrosslinked polymers polymers
Model of swelling gelModel of swelling gel
The The ““Silk Road Silk Road ”” TourTour
Populus euphraticaPopulus euphratica Oliv.(Oliv.(胡杨胡杨))
(Chen et al. 2001, 2002a, 2002b, 2003, 2004, Chang et al. 2006, Wang et al. 2007, 2008)
receptorPM
PM
Na+, Cl-
Na+,Cl-
Vacuole
Gene expression
Na+
H+
H+
Cl-
antioxidantenzymes
Ion compartmentation
ABA,Ca2+, CaM,
H2 O2
Na+
H+H+ K+,Na+ Cl-
ROS
SOD (SOD (superoxidesuperoxide dismutasedismutase) ) isoenzymesisoenzymes
P. euphratica P. popularisS S+H C S S+H C
SOD1
SOD2
SOD3
POD1
POD2
POD3
POD4
POD5
POD6
P. popularis P. euphraticaC S+H S C S+H S
S: NaCl; S+H: NaCl+hydrogel; C: control
POD (POD (peroxidaseperoxidase) ) isoenzymesisoenzymes