engr 1182.03 college of engineering engineering education innovation center engr 1182 nano pre-lab...
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Engr 1182.03
Presentation Short Title 1
College of EngineeringEngineering Education Innovation Center
Engr 1182 Nano Pre-Lab
Intro to Lab-on-a-chip
Rev: 20XXMMDD, Initials
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Engr 1182.03
Presentation Short Title 2
Lab-on-a-Chip• Small scale chemical lab in a portable and disposable
format.• Require smaller amounts of reagents• Disposable/Recyclable• Manufactured with inexpensive materials
Rev: 20XXMMDD, Initials
OSU Research
DNA analysis
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Engr 1182.03
Presentation Short Title 3
Biomedical Lab-on-a-Chip• A type of Lab-on-a Chip used for Biomedical
diagnostic tests specifically• Biomedical Lab-on-a-Chip is also called a “Point of care” device• Point of care:
– is defined as medical testing at or near the site of patient care– makes the test available to the patient immediately and conveniently – Therefore, physician receives the results quicker and can make
immediate clinical decisions• Examples for “point of care” devices
– Screening for infectious diseases in the emergency room– Forensic analysis at crime scenes– At home screening for HIV, Diabetes, or even Cancer
Rev: 20XXMMDD, Initials
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Engr 1182.03
Presentation Short Title 4
• Sample delivery/collection• Targeted compound
– Fluorescein *• Fluidics
– Fluidic channels in the chip• Detection interface
– Detection Alignment Device *• Analysis
– Electric circuit to interpret
detected data• Readout/Display
• Flashing LEDs*
* - These are specific to our Lab-on-a-Chip project.Rev: 20XXMMDD, Initials
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Engr 1182.03
Presentation Short Title 5
Micro vs. Nano
• Our chip is based on micro-fluidics and thus has micron size channels.
• Our chip has channel widths of 280-400 microns and a depth of 200 microns.
• There are no nano-fluidic devices yet on the market.• If we could manufacture nano-fluidic devices we might
be able to:– Use extremely small amount of samples– Control the flow of individual DNA or other biomolecules on
the chip.
Rev: 20XXMMDD, Initials
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Engr 1182.03
Presentation Short Title 6
Our Lab-on-a-chip project
• You will create 2 lab-on-a-chip designs that can detect and measure the concentration of fluorescein.
• What is fluorescein?
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Engr 1182.03
Presentation Short Title 7
Fluorescein
• Fluorescein in solution emits a “green” light when stimulated with “blue” light. This is called fluorescence.
• Since it isn’t toxic it can be used to track fluid flows and flow rates in the body.
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Engr 1182.03
Presentation Short Title 8
One Fluorescein Use
• A type of dye that is used in the study of the blood vessels in the eye. The test provides information about the circulatory system and the condition of the retina.
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Leakagebetweenvessels
UnderUltravioletLight
Retinal photograph of a patient complaining of decreased vision.Looks normal.
Fluorescein angiogram indicating fluid leakage within the retina
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Engr 1182.03
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Dry Eye detection
• Fluorescein is also used to determine tear flow rate in the eyes as a diagnostic tool for dry eye sydrome.
• After putting fluorescein in the eye, tears coming into the eye will reduce the fluorescein concentration over time.
• The rate of concentration change can be used to determine tear flow rate.
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Engr 1182.03
Presentation Short Title 10
Objective of Our Chip
• After fluorescein put in the eyes, tear samples are taken at intervals and the concentration must be determined for each sample.
• Our chips must be able to reliably measure the concentration of multiple sequential samples of fluorescein.
Read Project Description Document in the class materials for more details!
Rev: 20XXMMDD, Initials