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ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department of Energy 19901 Germantown Road Germantown, MD 20874-1290

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Page 1: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

ENERGY BIOSCIENCES

FY 1999 ANNUAL REPORT

Chemical Sciences, Geosciencesand Biosciences Division

Office of Basic Energy SciencesU.S. Department of Energy19901 Germantown RoadGermantown, MD 20874-1290

Page 2: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

FY 1999 ENERGY BIOSCIENCES ANNUAL REPORTThe Division of Energy Biosciences was provided approximately thirty-two million dollars in fiscal year 1999. Thebreakdown of how the resources were distributed is indicated in the following table.

Number FY 99 Percentof funding of

Projects (in thousands) totalfunds

University and Non-Profit 199 22,520 69Institutions

Michigan State UniversityPlant Research Laboratory 15 3,200 10

National Laboratories 11 2,340 7Brookhaven National LabLawrence Berkeley LabNational Renewable Energy Lab.

Small Business Innovation 3,934 12Research (SBIR) contribution,and Miscellaneous

Conferences, Educational 4 494 2Activities

229 $32,488

INVENTORY OF RESEARCH ACTIVITIES

U.S. Department of AgricultureDaniel R. Bush"Biochemical and molecular analysis of a new control pathway in assimilate partitioning"[email protected]$127,444 (two years)

U.S. Department of AgricultureJerry D. Cohen"Metabolic Regulation of the Plant Hormone Indole-3-acetic acid"[email protected] (moved to University of Minnesota FY 00)$94,000

U. S. Department of AgricultureSteven C. Huber"Role and Regulation of SNF-1-like Protein Kinases in Plant Carbohydrate Metabolism"DE-AI02-91 [email protected]$97,000

U.S. Department of AgricultureArchie R. Portis, Jr."Consequences of Altering Rubisco Regulation"

[email protected]$55,200

Energy Biosciences FY 1999 Annual Report

Page 3: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

U.S. Department of AgricultureSara Wright"Controls on production, incorporation and decomposition of glomalin -- a novel fungal soil protein important to soil carbon storage"[email protected]$66,491 (two years)

Arizona State UniversityRobert E. Blankenship"Structure, Function and Regulation of Antenna Complexes of Green Photosynthetic Bacteria"[email protected]$118,000

Arizona State UniversityWim Vermaas"Chlorophyll-Binding Proteins in the Cyanobacterium Synechocystis sp. PCC 6803"[email protected]$103,000

Arizona State UniversityAndrew N. Webber"Excitation energy transfer in the photosystem I core antenna: function of the clustered and connecting chlorophylls"[email protected]$200,000 (two years)

University of ArizonaHans J. Bohnert"Restructuring Metabolism for Photosynthesis Protection"[email protected]$101,999

University of ArizonaMartha C. Hawes"Role of Cell Wall Degrading Enzymes in the Programmed Separation of Cells from Root Caps"[email protected]$99,000

University of ArizonaRichard A. Jorgensen"Transgene silencing, paramutation, and promoter-homology-based control of gene expression"[email protected]$92,000

University of ArizonaBrian Larkins"Dissection of Molecular Mechanisms Regulating Protein Body Formation in Maize Endosperm"[email protected]$103,000

University of ArizonaBrian Larkins"Regulation of DNA Endoreduplication in Maize Endosperm"[email protected]$100,000

University of ArizonaKaren S. Schumaker"Molecular Characterization of the Role of a Calcium Channel in Plant Development"[email protected]$92,000

Energy Biosciences FY 1999 Annual Report

Page 4: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

University of ArizonaElizabeth Vierling"Cytosolic HSP100 Proteins and Stress Tolerance in Plants"[email protected]$100,000

Brookhaven National LaboratoryBenjamin Burr"Plant Molecular Genetics"[email protected]$352,000

Brookhaven National LaboratoryGeoffrey Hind"Regulation of Energy Conversion in Photosynthesis"[email protected]$325,000

Brookhaven National LaboratoryJohn Shanklin"Modification of Plant Lipids"[email protected]$300,000

Boston CollegeMary F. Roberts"Osmoregulation in Methanogens"DE-FG02-91 [email protected]$97,995

Boyce Thompson Inst. for Plant ResDavid B. Stern"Post-transcriptional Gene Regulation in Chloroplasts"[email protected]$91,000

Brown UniversitySamuel I. Beale"The Magnesium Branch of the Chlorophyll Biosynthetic Pathway"[email protected]$112,000

California Institute of TechnologyElliot Meyerowitz"Molecular and Genetic Analysis of LEAFY, a Gene Controlling Floral Induction and Flower Development in Arabidopsis thaliana"[email protected]$139,000

University of California, BerkeleyMichael Freeling"Genetically Programmed Cell Death in Maize"[email protected]$105,000

University of California, BerkeleyWilhelm Gruissem"Cellular Integration of MVA Synthesis and Protein Prenylation"[email protected]$120,000

Energy Biosciences FY 1999 Annual Report

Page 5: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

University of California, BerkeleyAndrew O. Jackson"Protein and RNA Interactions Involved in the Pathogenesis of Tomato Bushy Stunt Virus"[email protected]$80,400

University of California, BerkeleySteven E. Lindow"Determinants of Environmental Stress Tolerance by Bacteria on Leaves"[email protected]$83,586

University of California, BerkeleyPeter H. Quail"Phytochrome from Green Plants: Properties and Biological Function"[email protected]$107,000

University of California, BerkeleyBrian J. Staskawicz"Molecular Analysis of Pathogen Recognition and Signal Transduction Events Specifying Plant Disease Resistance"[email protected]$113,000

University of California, DavisJohn L. Bowman"Developmental Genetics of Nectaries in Arabidopsis and Gossypium"[email protected]$88,000

University of California, DavisDeborah P. Delmer"The Mechanism and Regulation of Cellulose Syntheses in Plants"[email protected]$105,000

University of California, DavisRoy H. Doi"Structure, Function and Assembly of the Clostridium cellulovorans Cellulosome"[email protected]$119,000

University of California, DavisJohn J. Harada"Regulation of Embryonic Development in Higher Plants"[email protected]$97,000

University of California, DavisWilliam J. Lucas"Cellular and Molecular Characterization of Vascular Plasmodesmata"[email protected]$122,000

University of California, DavisValley Stewart"Genetic Control of Nitrate Assimilation in Klebsiella oxytoca"[email protected]$87,387

Energy Biosciences FY 1999 Annual Report

Page 6: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

r ,i

University of California, DavisSteven Theg"Protein Import and Assembly in Chloroplasts"[email protected]$99,000

University of California, IrvineJanos K. Lanyi"Membrane Bioenergetics of Salt Tolerant Organisms"[email protected]$200,001

University of California, Los AngelesRobert B. Goldberg"Suspensor Differentiation During Early Plant Embryogenesis"[email protected]$105,000 (Sixteen and one-half months)

University of California, Los AngelesRobert P. Gunsalus"Molecular Biology and Genetics of the Acetate-Utilizing Methanogenic Bacteria"[email protected]$109,742

University of California, Los AngelesEduardo Zeiger"Sensory Transduction of the C02 Response of Guard Cells"[email protected]$101,000

University of California, San DiegoMaarten Chrispeels"The Signal Transduction Pathway of the Unfolded Protein Response"[email protected]$101,000 - FY 98 funds - two years

University of California, San DiegoJulian I. Schroeder"Physiology and Regulation of Calcium Channels in Stomatal Guard Cells"[email protected]$95,000

University of California, Santa CruzLincoln Taiz"Regulation of Vacuolar pH in Citrus limon"[email protected]$104,000

Carnegie Institution of WashingtonShauna C. Somerville"Powdery Mildew Disease Resistance"[email protected]$94,000

Carnegie Institution of WashingtonChris Somerville"Genetic Engineering of Biomaterials"[email protected]$531,509

Energy Biosciences FY 1999 Annual Report

Page 7: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

University of ChicagoRobert Haselkorn"Analysis of the Rhodobacter Genome Emphasizing Carbon Management"[email protected]$100,000

University of ChicagoDaphne Preuss"Cell-cell Interactions pollen tube growth in Arabidopsis"[email protected]$103,000

Cold Spring Harbor LaboratoryRobert A. Martienssen"The lojap Gene in Maize"[email protected]$100,000

University of ColoradoR. Ray Fall"Microbial Production of Isoprene"[email protected]$86,000

University of ConnecticutKenneth M. Noll"Sugar Transport and Metabolism in Thermotoga"[email protected]$89,000

University of ConnecticutWolf-Dieter Reiter"Genetic Analysis of Sugar Nucleotide Interconversions in Arabidopsis"[email protected]$90,000

Cornell UniversityMaureen Hanson"Energy Transduction in Plant Mitochondria"[email protected]$106,000

Cornell UniversityJames P. Shapleigh"Regulation of Denitrification in Rhodobacter sphaeroides"[email protected]$94,000

Cornell UniversityPeter L. Steponkus"Cold Acclimation of Herbaceous Species: Effect of Sugars on Membrane Cryostability"[email protected]$114,000

Cornell UniversityDavid B. Wilson"Studies of the Genetic Regulation of the Thermomonospora fusca Cellulase Complex"[email protected]$95,000

Energy Biosciences FY 1999 Annual Report

Page 8: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

University of DelawareJohn S. Boyer"Plant Growth with Limited Water"[email protected]$108,000

Donald Danforth Plant Science Ctr.Roger N. Beachy"Regulating Expression of Cell and Tissue-Specific Genes by Modifying Transcription"[email protected]$207,000 (two years)

East Tennessee State UniversityCarolyn M. Wetzel"Functional Analysis of Chloroplast Early Light Inducible Proteins (ELIPs)"[email protected]$108,944

Florida State UniversityWilliam H. Outlaw, Jr."Role of Sucrose in Modulating Stomatal Aperture"[email protected]$82,000

University of FloridaLonnie 0. Ingram"Ethanol-Tolerant Biocatalysts for Fuel Ethanol Production"[email protected]$100,000

University of FloridaDonald R. McCarty"Genetic Control of Abscisic Acid Biosynthesis in Plants"[email protected]$93,000

University of Georgia, AthensMichael W. W. Adams"The Metabolism of Hydrogen by Hyperthermophilic Microorganisms"[email protected]$110,000

University of Georgia, AthensPeter Albersheim"Structures and Functions of Oligosaccharins"[email protected]$170,000

University of Georgia, AthensPeter Albersheim and Alan Darvill"Center for Plant and Microbial Complex Carbohydrates"[email protected]$605,000

University of Georgia, AthensAlan Darvill"Structural Studies of Complex Carbohydrates of Plant Cell Walls"[email protected]$385,000

Energy Biosciences FY 1999 Annual Report

Page 9: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

University of Georgia, AthensJeffrey F.D. Dean"Structure-Function Relationships in Plant Laccases"[email protected]$90,999

University of Georgia, AthensLars G. Ljungdahl"Fermentation of Cellulose and Hemicelluloses by Clostridia and Anaerobic Fungi"[email protected]$170,000

University of Georgia, AthensRobert J. Maier"Roles of the Metal-Binding Protein Nickelin in Symbiotic Nitrogen fixation"[email protected]$106,000

University of Georgia, AthensRussell L. Malmberg"Genetic Analysis of Polyamine Synthesis in Arabidopsis"[email protected]$106,000

University of Georgia, AthensRichard B. Meagher"Mechanisms and Determinants of RNA Turnover: The Role of PAB2 in the Post-Transcriptional Apparatus"[email protected]$103,993

University of Georgia, AthensMichelle Momany"Identification of Novel Cell Wall Components"[email protected]$79,000

University of Georgia, AthensGregory W. Schmidt"Molecular and Physiological Studies of Photosynthetic Adaptation in Nitrogen Deficiency"[email protected]$200,000 (two years)

University of Georgia, AthensSusan Wessler"Structure, Regulation and Evolution of the R transcriptional activators from maize and rice"[email protected]$110,000

University of Georgia, AthensWilliam B. Whitman"Biochemistry and Genetics of Autotrophy in Methanococcus"[email protected]$90,000

University of Georgia, TiftonWayne W. Hanna"Development of Innovative Techniques and Principles That May Be Used as Models to Improve Plant Performance"[email protected]$56,955

Energy Biosciences FY 1999 Annual Report

Page 10: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

University of HawaiiDavid A. Christopher"Mechanisms regulating blue light-activated psbD transcription in plant chloroplasts"[email protected]$76,446

University of HawaiiHarry Y. Yamamoto"Xanthophyll Cycle and Photoprotective Systems in Higher Plants"[email protected]$95,000 FY 98 funds - two years

University of Illinois, ChicagoSimon Silver"Bacterial resistance to silver cations: molecular genetics, physiology and biochemistry"[email protected]$111,000

University of Illinois, UrbanaRobert B. Gennis"Studies on Cytochrome bo3 from E. coli"[email protected]$150,000

University of Illinois, UrbanaRalph S. Wolfe"Studies on the Microbial Formation of Methane"DE-FG02-87ER13651$82,746

Iowa State UniversityPhilip W. Becraft"Analysis of a signal transduction pathway involved in maize epidermis and aleurone differentiation"[email protected]$92,000

Iowa State UniversityAlan DiSpirito"Mechanism of Methane Oxidation in Cells Expressing the Membrane-Associated Methane Monooxygenase"[email protected]$93,224

Iowa State UniversitySteven R. Rodermel"Regulation of Carotenoid Biosynthesis: The immutans Mutant of Arabidopsis"[email protected]$86,000

University of IowaCaroline Harwood"Molecular Biology of Anaerobic Aromatic Biodegradation"[email protected]$92,000

Johns Hopkins UniversityRichard E. McCarty"Transport of Ions Across the Inner Envelope Membrane of Chloroplasts"[email protected]$104,000

Energy Biosciences FY 1999 Annual Report

Page 11: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

KAIROS Scientific IncDouglas C. Youvan"Macromolecular Scaffolds for Energy Transfer"[email protected]$136,000

University of KentuckyEdward DeMoll"Acetyl-CoA cleavage and synthesis in methanogens: Mechanistic, enzymological, and metabolic studies"[email protected]$97,000

University of KentuckySusheng Gan"Molecular Regulatory Mechanisms of Two Senescence-Specific Genes in Arabidopsis"[email protected]$93,000

University of KentuckyRobert L. Houtz"Mechanism and Significance of Post-Translational Modifications in the Large and Small Subunits of Ribulose BisphosphateCarboxylase/Oxygenase"[email protected]$88,195

Lawrence Berkeley National LaboratoryMark D. Alper"Biomolecular Materials"[email protected]$150,000

Lawrence Berkeley National LaboratoryJohn E. Hearst"Transferring Photosynthesis Genes to New Cellular Environments"[email protected]$197,000

Lawrence Berkeley National LaboratorySung-Hou Kim"Structural Basis of Plant Signal Transduction"[email protected]$117,000

Lawrence Berkeley National LaboratoryMelvin Klein"Mechanism of Water Splitting and Oxygen Evolution in Photosynthesis"[email protected]$210,000

Lawrence Berkeley National LaboratoryKenneth Sauer"Photosynthetic Pigment Proteins and Photosynthetic Light Reactions"[email protected]$216,000

Lawrence Berkeley National LaboratoryValerie Vreeland"Vanadium Haloperoxidase: Functional organization and regulation of catalysis in Fucus zygote adhesion"[email protected]$140,000

Energy Biosciences FY 1999 Annual Report

Page 12: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

Life Sciences Research FoundationDonald D. Brown"Developing Research Capabilities in Energy Biosciences"DE-FG02-89ER14025englar@mail .ciwemb.edu$369,000

Louisiana State UniversityTerry M. Bricker"Identification of Chloride-Binding Domains in Photosystem II"[email protected]$92,000

Marine Biological LaboratoryCaroline S. Harwood"Investigations into the Metabolic Diversity of Microorganisms as Part of Microbial Diversity"[email protected]$25,000

Marquette UniversityK. Dale Noel"Bacterial lipopolysaccharide structures required for root nodule development"[email protected]$93,000

University of MarylandCaren Chang"Suppressors and enhancers of an Arabidopsis ethylene receptor mutant"[email protected]$75,000

University of MarylandElisabeth Gantt"A New Pathway for Isopentenyl Pyrophosphate Synthesis in Bacteria and Plants"[email protected]$97,008

University of MarylandZhongchi Liu"Investigating the molecular mechanism of TSO1 function in Arabidopsis cell division and meristem development"[email protected]$94,000

University of MarylandKevin R. Sowers"Physiology and Genetics of Catabolic Metabolism in the Aceticlastic Methanogens"[email protected].$94,000

University of MarylandHeven Sze"Structure and Regulation of Calcium-Pumping ATPases and Plants"[email protected]$97,000

University of MassachusettsAlice Y. Cheung"Molecular Analysis of the Interactions Between Pollen Tube Components and a Stylar Transmitting Tissue Pollen Tube Growth-Promoting and Attracting Protein"[email protected]$96,000

Energy Biosciences FY 1999 Annual Report

Page 13: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

University of MassachusettsSusan B. Leschine"Microbial Fermentation of Abundant Biopolymers: Cellulose and Chitin"[email protected]$100,000

University of MassachusettsDerek R. Lovley"Mechanisms for Microbial Reduction of Humics and Structurally Related Compounds"[email protected]$94,000

Michigan State University/MSU-DOE Plant Research LaboratoryF.J. de Bruijn"Molecular Basis of Symbiotic Plant-Microbe Interactions"[email protected]$

Michigan State University/MSU-DOE Plant Research LaboratoryPamela J. Green"Molecular Mechanisms That Regulate the Expression of Genes in Plants"[email protected]$

Michigan State University/MSU-DOE Plant Research LaboratoryGregg A. Howe"Regulation of Plant Anti-herbivore Defense"[email protected]$

Michigan State University/MSU-DOE Plant Research LaboratoryHans Kende"Action and Synthesis of Plant Hormones"[email protected]$

Michigan State University/MSU-DOE Plant Research LaboratoryKenneth Keegstra"Chloroplast Biogenesis"[email protected]$

Michigan State University/MSU-DOE Plant Research LaboratoryKenneth Keegstra"Cell Wall Metabolism"$

Michigan State University/MSU-DOE Plant Research LaboratoryHans Kende"Photoperiodic Induction and the Floral Stimulus"$

Michigan State University/MSU-DOE Plant Research LaboratoryLee Mcintosh"Interaction of Nuclear and Organelle Genomes"[email protected]$

Michigan State University/MSU-DOE Plant Research LaboratoryN. V. Raikhel"Molecular Mechanisms of Trafficking in the Plant Cell"[email protected]$

Energy Biosciences FY 1999 Annual Report

Page 14: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

Michigan State University/MSU-DOE Plant Research LaboratoryJ. D. Walton"Biochemical and Molecular Aspects of Plant Pathogenesis"[email protected]$

Michigan State University/MSU-DOE Plant Research LaboratoryC. Peter Wolk"Developmental Biology of Nitrogen-Fixing Cyanobacteria"[email protected]$

Michigan State University/MSU-DOE Plant Research LaboratoryC.P. Wolk"Frankia Genetics"$

Michigan State University/MSU-DOE Plant Research LaboratoryJan A.D. Zeevaart"Environmental Control of Plant Development and Its Relation to Plant Hormones"[email protected]$

Michigan State UniversityChristoph Benning"Regulation of Thylakoid Lipid Head Group Biosynthesis in Arabidopsis thaliana"[email protected]$91,000

Michigan State UniversityJohn B. Ohlrogge"Biosynthesis of Triacylglycerol in Developing Oilseeds"[email protected]$102,863

Michigan State UniversityKen L. Poff"Minority Summer Research Program in the Plant Sciences"[email protected]$90,000

Michigan State UniversityJack Preiss"Structure-Function Relationships of ADP-Glucose Pyrophosphorylase: Manipulation of the Plant Gene for Increased Production ofStarch in Plants"[email protected]$90,000

Michigan State UniversityC. A. Reddy"Molecular Biology and Biochemistry of Basidiomycete Laccases"[email protected]$90,999

Michigan State UniversityJ. Gregory Zeikus"Molecular Physiology of Succinic Acid-Based Fermentations in Anaerobes: Control of Chemical Yield by C02 Fixation and ElectronDonors"[email protected]$90,000

Energy Biosciences FY 1999 Annual Report

Page 15: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

University of MichiganSteven E. Clark"CLV Signaling in Meristem Development"[email protected]$93,000

University of MinnesotaAlan B. Hooper"Biochemistry of Ammonia Monoxygenase of Nitrosomonas"[email protected]$105,000

University of MinnesotaSusan M. Wick"Reverse Transposon Tagging of Maize Tubulin Genes"[email protected]$110,000

University of MissouriTobias I. Baskin"Cellulose and the control of growth anisotropy"[email protected]$85,775

University of MissouriJames A. Birchler"Dosage Analysis of Gene Expression in Maize"[email protected]$107,000

University of MissouriJudy Wall"Genetics and Molecular Biology of Hydrogen Metabolism in Sulfate-Reducing Bacteria"[email protected]$95,000

University of MontanaMatthias C. Rillig"Controls on production, incorporation and decomposition of glomalin -- a novel fungal soil protein important to soil carbon storage"[email protected]$130,039 (two years)

Mount Sinai School of MedicineTerry Ann Krulwich"The Respiratory Chain of Alkalophilic Bacteria"[email protected]$114,000

NASA Ames Research CenterJonathan Trent"The molecular basis of hyperthermophily: the role of HSP60/chaperonins in vivo"[email protected]$100,000

National Renewable Energy LaboratoryMaria L. Ghirardi"Regulation of H2 and C02 Metabolism: 02 Sensor Involvement in Partitioning of Photosynthetic Reductant in Green Algae"[email protected]$49,000

Energy Biosciences FY 1999 Annual Report

Page 16: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

National Renewable Energy LaboratoryMichael Seibert"The Water-Splitting Apparatus of Photosynthesis"[email protected]$134,000

University of NebraskaT. Jack Morris"Protein and RNA Interactions Involved in the Pathogenesis of Tomato Bushy Stunt Virus"[email protected]$53,600

University of NebraskaStephen W. Ragsdale"Enzymology of Acetoclastic Methanogenesis"[email protected]$107,000

University of NebraskaRobert Spreitzer"Role of the Rubisco Small Subunit"[email protected]$94,000

New York UniversityGloria Coruzzi"Asparagine Synthetase Gene Regulation and Plant Nitrogen Metabolism"DE-FG02-92ER20071coruzg01 @mcrcr6.med.nyu.edu$111,000

North Carolina State UniversityRobert M. Kelly"Proteolysis in Hyperthermophilic Microorganisms"[email protected]$117,408

University of North CarolinaJeffery L. Dangl"Functions of the Pseudomonas syringae avrRpml Gene During Disease Resistance and as a Virulence Factor in Arabidopsis thalianaCell"[email protected]$132,000

University of North CarolinaSara R. Grant"Characterization of Arabidopsis Genes Involved in Gene Silencing"[email protected]$96,000

Ohio State UniversityCharles J. Daniels"The Role of Multiple TBP and TFB in Archaeal Gene Expression"[email protected]$101,000

Ohio State UniversityP. E. Kolattukudy"Biosynthesis of Hydrocarbons and Other Wax Components"[email protected]$100,000

Energy Biosciences FY 1999 Annual Report

Page 17: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

o S

Ohio State UniversityJoseph A. Krzycki"Transmethylation Reactions During Methylotropic Methanogenesis in Methanosarcina barkeri"[email protected]$98,000

Ohio State UniversityJohn N. Reeve"Regulation of Methane Genes and Genome Expression"[email protected]$122,000

Ohio State UniversityF. Robert Tabita"A Model System to Probe the Biochemistry and Molecular Control of a Globally Significant Alternative Mechanism to Sequester andMetabolize Carbon Dioxide"DE-FG02-91 ER20033tabita.1 @osu.edu$106,000

Ohio State UniversityF. Robert Tabita"Summer Workshop: Molecular Basis, Physiology, and Diversity of Microbial Adaptation"[email protected]$50,000

Oklahoma State UniversityAndrew Mort"The Structure of Pectins from Cotton Cell Walls"[email protected]$105,000

University of OklahomaMichael J. Mclnerney"Enzymology and Energetics of Syntrophic Benzoate Metabolism"[email protected]$94,999

Oregon Graduate Institute of Science & TechnologyJames M. Cregg"Regulation of Gene Expression by Methanol in the Yeast Pichia pastoaris"[email protected]$83,000

Oregon Graduate Institute of Science & TechnologyMichael Gold"Biochemical Genetics of Lignin Degradation by Phanerochaete chrysosporium"[email protected]$135,000

Oregon Graduate Institute of Science & TechnologyV. Renganathan"Cloning and Expression of Cellobiose Dehydrogenases"[email protected]$92,001

Oregon State UniversityDaniel J. Arp"Regulation of the Genes Involved in Nitrification"[email protected]$92,000

Energy Biosciences FY 1999 Annual Report

Page 18: ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT · ENERGY BIOSCIENCES FY 1999 ANNUAL REPORT Chemical Sciences, Geosciences and Biosciences Division Office of Basic Energy Sciences U.S. Department

University of OregonAlice Barkan"Genetic Analysis of Chloroplast Translation in Maize"[email protected]$200,000

Pennsylvania State UniversityJean E. Brenchley"The Characterization of Psychrophilic Microorganisms and Their Potentially Useful Cold-Active Glycosidases"[email protected]$106,000

Pennsylvania State UniversityDonald A. Bryant"Energy Transduction in Green Sulfur Bacteria"[email protected]$94,000

Pennsylvania State UniversityDaniel J. Cosgrove"Regulation of Plant Cell Growth: Structure and Function of Beta-Expansins in Rice and Maize"DE-FG02-84ER1 [email protected]$109,000

Pennsylvania State UniversityRichard J. Cyr"Elongation Factor 1Alpha and the Plant Cytoskeleton"[email protected]$96,000

Pennsylvania State UniversityJames G. Ferry"Enzymology of the Pathway for Acetate Conversion to Methane in Methanosarcina thermophila"[email protected]$108,000

Pennsylvania State UniversityJohn H. Golbeck'Primary Electron Transfer in Green Photosynthetic Bacteria"[email protected]$93,000

Pennsylvania State UniversityMark J. Guiltinan"Molecular-Genetic Analysis of Maize Starch Branching Enzyme Isoforms"[email protected]$94,000

Pennsylvania State UniversityMing Tien"Charcterization of Lignin and Mn Peroxidases from Phanerochaete chrysosporium"[email protected]$117,000

University of PennsylvaniaAnthony R. Cashmore"Light Responses and Photoperiodism in Arabidopsis thaliana"[email protected]$134,000

Energy Biosciences FY 1999 Annual Report

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University of PennsylvaniaFevzi Daldal"Membrane-Attached Electron Carriers in Photosynthesis and Respiration"DE-FG02-91 [email protected]$116,000

University of PennsylvaniaJoseph R. Ecker"Molecular and Genetic Analysis of Hormone-Regulated Differential Cell Elongation in Arabidopsis"[email protected]$103,000

University of PennsylvaniaScott Poethig"The function of the EARLY TRICHOMES gene in Arabidopsis in maize"[email protected]$100,000

University of PennsylvaniaPhilip A. Rea"Biochemical Basis of YCF1-Dependent Vacuolar Glutathione-S-Conjugate Transport"DE-FG02-91 [email protected]$100,000

Purdue UniversityNicholas Carpita"Mechanisms of synthesis of mixed- linkage B-glucan, and the CeSA gene family of rice"[email protected]$99,000

Purdue UniversityClinton C. S. Chapple"Ferulate-5-hydroxylase: requirements for expression and activity"[email protected]$195,000

Purdue UniversityDavid Rhodes"Engineering Plant One-Carbon Metabolism"[email protected]$37,738

Purdue UniversityLouis Sherman"The Impact of Environmental Stress on the Regulation of Photosynthesis"[email protected]$220,000 (two years)

Purdue UniversityChristopher J. Staiger"Identification of Actin-Binding Proteins from Maize Pollen"[email protected]$96,000

Rensselaer Polytechnic InstituteJackie L. Collier"A Novel, Photosynthesis-Associated Thioredoxin-Like Gene"[email protected]$209,000 (two years)

Energy Biosciences FY 1999 Annual Report

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Rice UniversityJanet Braam"Complex Regulatory Controls of TCH Gene Expression"[email protected]$100,000

University of RochesterJ.H. David Wu"The Structure-Function Relationship of the Clostridium thermocellum Cellulosomal Dockerin"[email protected]$82,000

The Rockefeller UniversityNam-Hai Chua"Function of Rac GTPases in Plants"[email protected]$99,000

Rutgers UniversityPaul Falkowski"Molecular Bases and Photobiological Consequences of Light Intensity Adaptation in Photosynthetic Organisms"[email protected]$90,000

Rutgers UniversityJoachim Messing"Corn Storage Protein - A Molecular Genetic Model"[email protected]$116,000

The Salk Institute for Biological StudiesJoanne Chory"Signal Transduction Pathways that Regulate CAB Gene Expression"[email protected]$116,000

The Salk Institute for BiologicalStudiesDetlef Weigel"Regulation of the floral homeotic gene AGAMOUS"[email protected]$101,000

The Scripps Research InstituteJeffrey F. Harper"Membrane Targeting of P-type ATPases in Plant Cells"[email protected]$98,000

The Scripps Research InstituteStephen P. Mayfield"Nuclear Genes Regulating Translation of Organelles mRNAs"[email protected]$99,257

University of South CarolinaBeth A. Krizek"Regulatory role of ANT in organ initiation and growth"[email protected]$95,000

Energy Biosciences FY 1999 Annual Report

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Southern Illinois UniversityDavid P. Clark"Regulation of Alcohol Fermentation by Escherichia coli"[email protected]$100,000

Stanford UniversitySharon R. Long"R. meliloti-Medicago nodulation genes and siginals: genetic and genomic approaches"[email protected]$274,000

University of TennesseeFred C. Hartman"Rubisco Mechanism: Dissection of the Enolization Partial Reaction"[email protected]$200,000 (two years)

University of TennesseeGary Stacey"Plant recognition of rhizobial Nod factors"[email protected]$84,000

University of TennesseeAlbrecht G. von Arnim"Mechanism of Regulated Protein Transport between Nucleus and Cytoplasm"[email protected]$99,000 FY 98 funds - two years

Texas A&M UniversityHagan P. Bayley"Novel Biomaterials: Genetically Engineered Pores"[email protected]$82,500(funded jointly with Division of Materials Sciences)

Texas A&M UniversityJames W. Golden"Regulation of Development and Nitrogen Fixation in Anabaena"[email protected]$93,000

Texas A&M UniversityJohn E. Mullet"Regulation of Chloroplast Division in Higher Plants"[email protected]$96,000

Texas A&M UniversityKathryn A. VandenBosch"Mutants of the Legume Medicago truncatula Defective in Root Hair Development and Infection by Rhizobium"[email protected]$117,000

Energy Biosciences FY 1999 Annual Report

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Texas Tech UniversityRichard L. Blanton"The Dictyostelium discoideum Cellulose Synthase: Structure/Function Analysis and Identification of Interacting Proteins"[email protected]$100,000

Texas Tech UniversityDavid B. Knaff"Ferredoxin-Linked Chloroplast Enzymes"[email protected]$199,700 (two years)

University of TexasR. Malcolm Brown, Jr."Structural and Functional Analysis of the Cellulose-synthesizing Complex in Vascular Plants"[email protected]$96,000

University of TexasKaren S. Browning"Phosphorylation of Plant Protein Synthesis Initiation Factors"[email protected]$92,000

University of TexasMark Estelle"Function of the Arabidopsis TIR1 gene in auxin response"[email protected]$93,999

TIGR - The Institute for Genomic ResearchC. Robin Buell"Sequencing of Chromosome 10 of Rice and Validation of Annotation Methods for Rice"[email protected]$300,000

TIGR - The Institute for Genomic ResearchKaren A. Ketchum"Tandem-pore outward-rectifying K+ channels: Molecular partners of the proton ATPase in membrane potential regulation"[email protected]$96,000

TIGR -The Institute for Genomic ResearchJ. Craig Venter"Sequencing of Arabidopsis Chromosome 2 (and Beyond), and Development of Resources for Arabidopsis Genome Analysis"[email protected]$249,999 FY 98 funds - two y ears

Virginia Polytechnic Institute & State UniversityJiann-Shin Chen"Enzymology of Acetone-Butanol-lsopropanol Formation"[email protected]$103,000

University of VirginiaMichael P. Timko"Protein Structure in Catalytic Function of NADPH: Protochlorophyllide Oxidoreductases"[email protected]$98,000

Energy Biosciences FY 1999 Annual Report

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Washington State UniversityJohn A. Browse"Lipid Signaling and Membrane Function in Mutants of Arabidopsis"[email protected]$131,000 (13 months)

Washington State UniversityRodney Croteau"Regulation of Terpene Metabolism"[email protected]$110,000

Washington State UniversityMichael L. Kahn"Carbon Metabolism and Dinitrogen Reduction in Symbiotic Nitrogen Fixation"[email protected]$98,000

Washington State UniversityDavid M. Kramer"The Energy Budget for Steady-State Photosynthesis"[email protected]$97,000

Washington State UniversityNorman G. Lewis"A New Perspective on Phenoxy Radical Coupling Reactions in vivo and Phenylpropanoid Pathway Regulation"[email protected]$109,500

Washington State UniversityNorman G. Lewis"Washington State University Interdisciplinary Plant Biochemistry Research and Training Center"DE-FG06-94ER20160$163,000

Washington State UniversityNorman G. Lewis"An Advanced Course in Plant Biochemistry"DE-FG03-96ER20247$33,000 FY 98 funds - two years

Washington State UniversityThomas Okita"Enhancement of Photoassimilate Utilization by Manipulation of ADPGlucose Pyrophosphorylase"[email protected]$97,000

Washington State UniversityJohn C. Rogers"Targeting and Processing of the Thiol Protease Aleurain"[email protected]$98,000

Washington UniversityHimadri B. Pakrasi"Biogenesis of Photosystems in Synechocystis 6803, a cyanobacterium"[email protected]$199,000 (two years)

Energy Biosciences FY 1999 Annual Report

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University of WashingtonMary Lidstrom"Genetics in Methylotrophic Bacteria"[email protected]$106,000

University of WisconsinSebastian Y. Bednarek"Identification and characterization of Arabidopsis thaliana cell-plate proteins"[email protected]$75,000

University of WisconsinAnthony B. Bleecker"Genetic Analysis of Ethylene Perception and Signal Transduction in Arabidopsis"DE-FG02-91 [email protected]$99,000

University of WisconsinDaniel Cullen"Molecular Genetics of Ligninase Expression"[email protected]$110,000

University of WisconsinTimothy J. Donohue"Microbial Formaldehyde Oxidation"[email protected]$94,000

University of WisconsinKenneth E. Hammel"One-electron oxidative mechanisms for lignocellulose decay by fungi"[email protected]$82,000

University of WisconsinJerry L. Kermicle"Epigenetic Silencing of the Maize r Gene"[email protected]$90,000

University of WisconsinPaul W. Ludden"The Biochemistry, Bioenergetics, and Physiology of the CO-Dependent Growth of Rhodospirillum rubrum"[email protected]$103,000

University of WisconsinThomas Sharkey"Starch Conversion to Sucrose in Plant Leaves"[email protected]$243,000 (two years)

University of WisconsinMichael R. Sussman"Molecular Mechanism of Energy Transduction By Plant Membrane Proteins"[email protected]$112,000

Energy Biosciences FY 1999 Annual Report

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University of WisconsinRichard Vierstra"Post-Translational Regulation of Phytochrome Action"[email protected]$110,000

Xavier University of LouisianaRobert Blake II"Molecular Characterization of Bacterial Respiration on Minerals"[email protected]$110,515

Yale UniversityClifford L. Slayman"Calcium Mapping and Signalling in Yeast"[email protected]$100,000

Yale UniversityDieter Soil"Essential Cysteine Metabolism in Archaea"[email protected]$112,000

Energy Biosciences FY 1999 Annual Report