elisa tests
TRANSCRIPT
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ELISAvirology
Hamid Afkhami
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EL Enzyme-LinkedEnzyme catalysis the substrate and produce colored species.
IS ImmunoSorbentAntigen or antibody are sticked in wells
About ELISA
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Types of the ELISA1. Direct2. Indirect3. Sandwich4. competitive
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Direct ELISASTEPS:
Antigen stick to well with charge + casein or albumin(BSA).
Add sample to well Wash Add substrate acid measure OD
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Advantages Quick Eliminate Cross-
reactivity of secondary Ab
Disadvantages the signal is less
amplified Labeling has destructive
effects on ab and consuming time.
Direct ELISA
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STEPS: Antigen stick to well with
charge + casein or albumin(BSA).
Add sample + Wash well Add detector ab + wash Add substrate and
measure OD
Indirect ELISA
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Advantages: High sensitivity Flexibility This format of indirect ELISA is
suitable for determining total antibody level in samples
Cost saving Several Epitopes for secondary
Ab and signal amplification
Indirect ELISA
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Antigen detection STEPS:
Stick ab to well + BSA Add sample (ag) + wash Add detector ab + wash Add substrate + measure OD
Advantages High specificity Suitable for complex samples
SANDWICH ELISAcontain at least two antigenic
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COMPETITIVE ELISA
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COMPETITIVE ELISA
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Advantages Suitable for complex samples Flexibility and sensitivity
COMPETITIVE ELISA
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http://www.elisa-antibody.com/ELISA-Introduction https://www.bio-rad-antibodies.com https://www.youtube.com http://arian-andish.com https://en.wikipedia.org/wiki/ELISA
Sources
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Have a nice day