effectiveness of bispecific cytotoxin (dtegfatf) in ameliorating human glioblastoma tumors
DESCRIPTION
Effectiveness of Bispecific Cytotoxin (DTEGFATF) in Ameliorating Human Glioblastoma Tumors. Travis Spangler 2008-2009. Research Problem. http://upload.wikimedia.org/wikipedia/commons/c/c0/Glioblastoma_-_MR_sagittal_with_contrast.jpg. Background: Stish et al. (2007) . DT EGF 13. - PowerPoint PPT PresentationTRANSCRIPT
Effectiveness of Bispecific Cytotoxin (DTEGFATF) in Ameliorating Human Glioblastoma Tumors
Travis Spangler2008-2009
Research Problem
http://upload.wikimedia.org/wikipedia/commons/c/c0/Glioblastoma_-_MR_sagittal_with_contrast.jpg
Background: Stish et al. (2007)
Diphtheria toxin epidermal growth factor 13
DTEGF13
DTEGF13
DTEGFIL13DTIL13
Diphtheria toxin amino acid transfer
Diphtheria toxin epidermal growth factor
Background: Stish et al. (2007)
Diphtheria toxin epidermal growth factor 13
DTEGF13
DTEGFATF ATF
Diphtheria toxin epidermal growth factor amino terminal fragment
DTEGFATF ATF
DTEGFATFDTAT
Diphtheria toxin amino acid transfer
Diphtheria toxin epidermal growth factor
Background: Stish et al. (2008)
DTEGF
Background: Stish et al. (2008)
DTEGF
Background: Vallera et al. (2002)
DTAT
Background: Vallera et al. (2002)
DTAT
http://www.biooncology.com/bioonc/images/her-main.jpg
Receptor
http://www.biooncology.com/bioonc/images/her-main.jpg
Receptor
Receptor
Receptor
Receptor ReceptorReceptor
Receptor
Receptor
Receptor
• DTEGFATF• DTEGF• DTAT
• DTEGFATF• DTEGF• DTAT• DTEGF + DTAT
Cell Lines
U-87 HUVEC HPB-MLT
http://www.assaysoft.com/gallery/02Microscopy/MicroscopyGallery/12/Human%20Umbilical%20Vein%20Endothelial%20Cells%20(HUVEC).jpghttp://data-integrator.com
http://www.hpb.org.uk/userimages/panfigure2.jpg
Cell Lines
U-87 HUVEC HPB-MLT
http://www.assaysoft.com/gallery/02Microscopy/MicroscopyGallery/12/Human%20Umbilical%20Vein%20Endothelial%20Cells%20(HUVEC).jpghttp://data-integrator.com
http://www.hpb.org.uk/userimages/panfigure2.jpg
Cell Lines
U-87 HUVEC HPB-MLT
http://www.assaysoft.com/gallery/02Microscopy/MicroscopyGallery/12/Human%20Umbilical%20Vein%20Endothelial%20Cells%20(HUVEC).jpghttp://data-integrator.com
http://www.hpb.org.uk/userimages/panfigure2.jpg
Cell Lines
U-87 HUVEC HPB-MLT
http://www.assaysoft.com/gallery/02Microscopy/MicroscopyGallery/12/Human%20Umbilical%20Vein%20Endothelial%20Cells%20(HUVEC).jpghttp://data-integrator.com
http://www.hpb.org.uk/userimages/panfigure2.jpg
Proliferation Assays
Proliferation Assays
Proliferation Assays
Proliferation Assays
Proliferation Assays
Proliferation Assay Results
Proliferation Assay Results
Proliferation Assay Results
Proliferation Assay Results
Proliferation Assay Results
Proliferation Assay Results
Proliferation Assay Results
Blocking Assay
http://www.biooncology.com/bioonc/images/her-main.jpg
Blocking Assay
http://www.biooncology.com/bioonc/images/her-main.jpg
Blocking Assay
DTEGFATF
http://www.biooncology.com/bioonc/images/her-main.jpg
DTEGFATF
DTEGFATF
DTEGFATF DTEGFATF DTEGFATFDTEGFATF
DTEGFATF
DTEGFATF
DTEGFATF
Blocking Assay
http://www.biooncology.com/bioonc/images/her-main.jpg
Blocking Assay Results
Blocking Assay Results
Limitations
• Blocking assay– Bispecific cytotoxin disperses throughout tumors– Concentration of antibodies
Future Work
• Clinical trial
Acknowledgements• Dr. Daniel Vallera• The University of Minnesota• Vallera Lab• Lois Fruen• Advanced Science Research
Effectiveness of Bispecific Cytotoxin (DTEGFATF) in Ameliorating Human Glioblastoma Tumors
Travis Spangler2008-2009