effect of a microbial 6 phytase supplementation on plasmatic myoinositol, and on the pectoralis...
TRANSCRIPT
EFFECT OF A MICROBIAL 6-PHYTASE SUPPLEMENTATION ON PLASMATIC MYO-INOSITOL AND ON THE PECTORALIS MUSCLE TRANSCRIPTOME IN BROILERS
J. Schmeisser1, R. Aureli1, A. Friedel2, A-A. Séon1, P. Guggenbuhl1, A. Cowieson2 and F. Fru.
1DSM Nutritional Products France, Centre de Recherche en Nutrition Animale, BP 170, 68305 Saint-Louis Cedex, France2DSM Nutritional Products Ltd, Kaiseraugst, Switzerland
Phytase efficiency to increase phosphorus utilization inpoultry has been proven for decades. In addition,phytase was demonstrated to improve growthperformance, meat breast weight, amino acidsdigestibility and plasma myo-inositol concentration.The objective of this work was to investigate potentialinteractions between phytase supplementation, growthperformance and host gene expression to identifypotential associated biomarkers.
Animals:
Diets:
Parameters:
- 16 broilers per treatment fed a corn/soybean mealbased diet ad libitum 36 days
- Negative control (NC) - Pi deficient (Total P=0.44%)- Positive control (PC) – Pi sufficient (Total P=0.75%)- NC + RONOZYME® HiPhos at 1000 U/kg- Transcriptomic analysis of breast muscle (Pectoralis
major) with Affymetrix CHIGENE-1.0-ST arrays(4 pools of 4 samples per treatment) and validationof selected genes by Q-PCR (n=16).
• Specific biomarkers potentially related to phytase supplementation havebeen identified in muscle.
• The results highlighted the importance of IP3 and myo-inositol (end productsof phytic acid degradation by phytase) for improving muscle growth.
• Confirm these results in other farm animal species and study the host geneexpression pattern in relation to other feed enzymes.
Most impacted pathways in animals fed RONOZYME® HiPhos:• Development - Calcium/calmodulin-dependant kinase and IGF
(via myo-inositol stimulation) controlling skeletal myogenesis
Number of modulated genes compared to negative control(with a minimum fold-change of 1.3 or -1.3 and p<0.05):
Fold-change compared to negative control
Microarray (n=4x4) Q-PCR (n=16)Gene symbol
HiPhos1000 PC
HiPhos1000 PC
PPP3CBMEF2AMEF2CPLCB1PI3KR1
1,67*1,44*1,43*1,432,02**
1,181,081,171,021,32
2,67*1,68*2,14*2,66*3,04*
1,881,421,731,481,51
Calcium/calmodulin-dependant kinase and IGF controlling skeletal myogenesisand IP3 signaling
* p< 0,05 ** p< 0,01
IGF-1receptor
IGF-1
PIP2
PIP3
PIP2IP3
CalciumCalmodulin (CALM1)
Myocyte enhancerfactor 2 (MEF2)
K(lysine)acetyltransferase 2B
(KAT2B)
Myogenic regulatory factor
(MYOD)
SKELETAL MUSCLE HYPERTROPHY SKELETAL MUSCLE DIFFERENCIATION
Myo-inositol
Myo-INO Phosphoinositide-3-kinase(PIK3R1 & PIK3CA)
Protein phosphatase 3 (PPP3CB)
Calcineurin A
IP3 receptor3-phosphoinositide dependent protein kinase-1 (PDPK1)
Pkb/AktPathway
Ribosomal protein S6 kinase b-1 (RPS6KB1)
PROTEIN SYNTHESIS
IGF binding protein (IGFBP)
Phospholipase C bêta (PLCB)
Up-regulated genes with phytase
PC vs NC HiPhos1000 vs NC205 53095
Confirmation of expression of selected genes by Q-PCR:
0
500
1000
1500
2000
2500
3000
PC NC HiPhos 1000
Wei
ght
gain
(g)
0,050,0
100,0150,0200,0250,0300,0350,0
Brea
st w
eigh
t (g
)
PC NC HiPhos 1000 0,010,020,030,040,050,060,070,0
Myo
-ino
sito
l (m
g/L)
PC NC HiPhos-1000
Myo-inositol effects on insulin sensitivity
ab b
a aaa
bb
a,b: Means within the chart with common superscripts do not differ significantly (p<0.05)