ecoguard(oil-degrading microbial agent)english
TRANSCRIPT
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Ecophile Co., Ltd
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Items Our product Other product Remark
1. Microbes mixMix by hydrocarbon
intake mechanism
Mix by oil-degrading
capacity
Harmony with native microbes
(Environment-friendly )
2. Feasibility of
weathered oil
Adding the microbesdegrading metabolite,
Adding the microbesdegrading lubricant
-Excellent feasibility in chroniccontaminated soil and lubricant
contaminated soil
3. Condition of
large scale culture
Adding thehydrocarbon
Adding generalnutrients
Excellence of initial oil-degrading velocity
Characteristics
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The products are officially certified by the MOE (Ministry of Environment) and KCG
(Korea Coast Guard) on efficiency & safety
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EfficiencyEfficiency--increasing technology by hydrocarbon uptake mechanism of oilincreasing technology by hydrocarbon uptake mechanism of oil--degrading microbesdegrading microbes
0
0. 2
0. 4
0. 6
0. 8
1
1. 2
1. 4
1. 6
0 20 40 60 80 100
ydrophobicity %
EmulsifyingActi
ity
D
at610nm
WL- 1
WL
- 1
WL
- 2
WL
- 3
WL- 2
DJ-1
DJ-3
L180
K
3- 2
DJ-2
YS- 7
- 10
0
0. 2
0. 4
0. 6
0. 8
1
1. 2
1. 4
1. 6
0 20 40 60 80 100
ydrophobicity %
EmulsifyingActi
ity
D
at610nm
WL- 1
WL
- 1
WL
- 2
WL
- 3
WL- 2
DJ-1
DJ-3
L180
K
3- 2
DJ-2
YS- 7
- 10
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Group 1 Group 2 Group 3 Group 4
Group 1
Group 2 23 5
Group 3 4
41
Group 4 9
91 93
Division
trains CharacteristicUptakeMode
Group 1
H-2,3
DJ-3EA o, HP q
Pseudo-solubilized
Group 2
-1, 2 EA q, HP oDirectContact
Group 3
H3-2, DJ-2 EA q, HP q
olubilized
Group 4
H-1, DJ-1
C
1
, IC-1
-7
MIXED MIXED
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Adding of metaboliteAdding of metabolite--degrading microbesdegrading microbes
-- TwoTwo microbes deposit (oversea) ,microbes deposit (oversea) , -- Patent pending/registrationPatent pending/registration
!
+"
2
+ N/P +
!
+"
2
+ N/P +
Biotrans formation
(Acids ,#
etons ,Alco hols ..)
Biotrans formation
(Ac ids ,#
etons ,Alco hols ..)
$
es id% e s
(:!
& clics , PA
s ..)
$
es id% e s
( :!
& clics , PA
s ..)
'
ineralization
! "
2 +
2
"
'
inera lizatio n
! "
2 +
2
"BiomassBiomas s
Fee dback
Inhibition
!
+"
2
+ N/P +
!
+"
2
+ N/P +
Biotrans formation
(Acids ,#
etons ,Alco hols ..)
Biotrans formation
(Ac ids ,#
etons ,Alco hols ..)
$
es id% e s
(:!
& clics , PA
s ..)
$
es id% e s
( :!
& clics , PA
s ..)
'
ineralization
! "
2 +
2
"
'
inera lizatio n
! "
2 +
2
"BiomassBiomas s
Fee dback
Inhibition
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SA AR
IS
SA AR
IS
SA AR
IS
ASP
SA AR
IS
ASPA) Initial
(
) After 18 days
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FinalFinal oiloil--degradingdegrading raterate ((1616 days)days))
A 0 R)
A 0 R ((5959..2211
))
InIn thethe casecase ofof metabolitemetabolite--degradingdegrading microbesmicrobes adding,adding, TPHTPH concentrationconcentration waswas reducedreduced asas
averageaverage 566566ppmppm everydayeveryday..
CostCost reductionreduction byby shortenshorten treatmenttreatment periodperiod
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Test itemsTest items UnitsUnits Standard valueStandard value Result valueResult value RemarkRemark
OilOil--degrading capacitydegrading capacity
(TPH)(TPH)mg/kgmg/kg
Soil Environment Conservation2
awSoil Environment Conservation2
awee
3
a3
a 44 regionregion
(2
ess than 2,000mg/kg)(2
ess than 2,000mg/kg)
2
ess than 600mg/kg2
ess than 600mg/kg
5
orea resources5
orea resources
corporationcorporation
Measurement o 4 totalMeasurement o 4 total
microbes3
o.microbes3
o.CFU/mlCFU/ml > 10> 1088 2.4 X 102.4 X 101010
5
orea Testing and5
orea Testing and
Research InstituteResearch Institute
Measure o 4 oilMeasure o 4 oil--degradingdegrading
microbes3
o.microbes3
o.MP
3
/mlMP3
/ml 5X105X1077 ~ 10~ 1088 5.2 X 105.2 X 1099 SelfSelf--test resulttest result
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After sampling, suspension
according to each dilution
rate
Measurement of total
microbial6
o.:
Spread on the agar plate
Oil-degrading microbial6
o.:
Inoculated on 96 well micro
plate
- Total microbial6
o :
Cultured on 30 for 3days
- Oil-degrading microbial6
o :
Cultured on 30 for 10days
Total microbial6
o. :
Colony6
o.
(Units : CFU/g-soil)
Oil-degrading microbial6
o :
( : MP6
/g-soil)Oil-degrading activity is indicated by red color.
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Sample extraction Sample sonication Sample Cleaning Sample
concentration
Adding internal
standard
Sample7
oading Data analysis Results
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(A) Sample roading, (8
) Sample developing, (C) Evaporation of solution,(D) Rod hold, (E) Analysis screen, (F) T
9
C/FID system
Fig. T9
C/FID analysis of fractionated resin componentseparated by modified method
Primary developing
Secondary developing
Tertiary developing
Developing solution: n-hexaneAliphatic : 10cm developing
Developing solution : n-hexane : toluene(1:4)Aromatic : 5cm developing
Developing solution :dichloromethane:methanol(19:1)Resin developing
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0
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30
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60
70
80
90
Diesel-contaminated soil
Crude oil-contaminated soil
Lubricant oil-contaminated soil
Crude oil-contaminated soil (the coast)
0
10
20
30
40
50
60
70
80
90
100
Ga region Na region Da region
A Co. (Domestic)
F Co. (Foreign)
EcoGuard
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Division Contents Remark
Development
periods
-Ministry of Environment (G7 Project, @ 92. ~ c 02)
(A
ORDI, Technology transfers)
-Ministry of Environment (Eco-technopia 21 , @ 01 ~ @ 09)
-Ministry of Commerce (Project, @ 00 ~ @ 02)
-Ministry of Science an @ Technology (Project, @ 01 ~ @ 02)
Development costs\1,620,000,000
(A
ORDI @ evelopment costs : \2,200,000,000)
A
orean 100B
Manpower 8 personnel (Total 65 personnel input)
Designer 2 personnel (Facility @ esign)
TechnologyC
evelopment