ecoelectrica plankton and entrainment analysis (2)

Analysis of zooplankton in Guayanilla-Tallaboa Bays complex.

An Evaluation of Zooplankton entrainment by the cooling water intake system operated by

EcoElectrica, LP.

A Report Prepared by

Ernesto Otero Department of Marine Science

Isla Magueyes University of Puerto Rico

Lajas, Puerto Rico

And

Áurea E. Rodriguez [email protected]

Department of Biology University of Puerto Rico, Río Piedras

30 April 2013

BMPP 2012/Zooplankton/Entrainment of 101

Ernesto Otero/DMS/UPRM

Table of Contents Table of Figures ....................................................................................................................................... 4

Abstract .................................................................................................................................................. 5

Introduction ............................................................................................................................................ 6

Methods .................................................................................................................................................. 8

Study Sites ........................................................................................................................................... 8

Sampling Days and Periods .................................................................................................................. 8

Sample Collection ................................................................................................................................ 9

Evaluation of Plankton Abundance ...................................................................................................... 9

Entrainment Estimates ...................................................................................................................... 10

Results and Discussion ........................................................................................................................... 12

Spatial distribution of zooplankton throughout Guayanilla Bay .......................................................... 12

Day-night changes of zooplankton in areas associated to EcoElectrica’s pier including intake and

outfall sites ........................................................................................................................................ 14

Zooplankton community composition................................................................................................ 14

Estimates of Entrainment on total zooplankton, fish eggs, fish larvae and potential adult fishes. ....... 15

Conclusions ........................................................................................................................................... 17

References ............................................................................................................................................ 20

Figures .................................................................................................................................................. 21

Apendices.............................................................................................................................................. 33

Appendix 1. Sampling Sheets ............................................................................................................. 34

Appendix 2. Zooplankton Counts ....................................................................................................... 38

Verification of Ichthyoplankton Counts .......................................................................................... 76

Appendix 3. Statistical Analyses ......................................................................................................... 77

SUMMARY OF MULTIPLE COMPARISON ANALYSIS OF TOTAL ZOOPLANKTON ABUNDANCE AMONG

DIFFERENT STATIONS ..................................................................................................................... 77

SUMMARY OF MULTIPLE COMPARISONS ANALYSIS OF HOLOPLANKTON ABUNDANCE MINUS

CALANOIDS ABUNDANCE AMONG DIFFERENT STATIONS. .............................................................. 78

SUMMARY OF MULTIPLE COMPARISONS ANALYSIS OF MEROPLANKTON ABUNDANCE AMONG

DIFFERENT STATIONS. .................................................................................................................... 79



BRAY CURTIS HIERARCHICAL CLUSTER ANALYSIS USING HOLOPLANKTON(NO CALANOIDS) AND

MEROPPLANKTON RESULTS. .......................................................................................................... 80

TWO WAY ANALYSIS OF VARIANCE EVALUATING STATISTICAL DIFFERENCES BETWEEN PLANKTONIC

PLANKTONIC GOUPS DURING DAY AND NIGHT SAMPLINGS. .......................................................... 81

Appendix 4. Results of Different Entrainment Scenarios using plankton concentrations from differnet

stations. ............................................................................................................................................ 85

Zooplankton Estimates.................................................................................................................. 86

Fish larvae ..................................................................................................................................... 89

Fish Eggs ........................................................................................................................................ 92

Potential Adult Fish ........................................................................................................................ 97



Table of Figures

Figure 1. Zooplankton sampling sites (13). The blue square contains sites sampled during the

night of 11 Oct 2012. North is towards the top of the figure. ...................................................... 22

Figure 2. Average total zooplankton abundance divided in holo and meroplankton. Samples are

arranged in order of total abundance .......................................................................................... 23

Figure 3. Abundance of Holoplankton minus calanoids (Top) and and Meroplankton (Bottom).

Error bars represent 1 SD. ............................................................................................................ 24

Figure 4. Summary results from hierarchical cluster analysis of Holoplankton-minus calanoids

using Bray-Curtis Distances and Nearest Neighbor Clustering (segmented polygon) over the

representation of holoplankton minus calnoids vs meroplankyon average abundance. ............ 25

Figure 5. Average total, holo- and meroplankton abundance for stations sampled during night

and day. A two way analysis of variance indicated no overall statistically significant differences

between day and night samples at these stations. However, the interaction term was significant

thus such difference is dependent on stations. Holoplankton increased during the night at

station 1 and 5 while meroplankton increase was mainly observed at station 5. ....................... 26

Figure 6. Percent composition of the 24 zooplankton groups found in > 30% of the stations. The

abundance used as the base for calculation is 2374 ind m-3 ; H= holoplankton; M=

meroplankton. .............................................................................................................................. 27

Figure 7. Principal component analysis using zooplankton groups present in >30% of all stations.

Red dots represent the strength of each station along the axes of the first 2 PC while lines

represent the relative strength of each group of plankton along the PC gradient. Lines towards

one of the stations symbol indicate plankton groups more strongly associated to that station. 28

Figure 8. Frequency distribution of the proportion of entrained zooplankton to that of the total

Guayanilla Bay community. The numbers over the bars are the proportions for each %

entrainment range. ....................................................................................................................... 29

Figure 9. Frequency distribution of the proportion of entrained fish eggs to that of the total



Figure 10. Frequency distribution of the proportion of entrained fish larvae to that of the total



Figure 11. Frequency distribution of the proportion of entrained potential adult fishes to that of

the total Guayanilla Bay community. The numbers over the bars are the proportions for each %




Abstract

Zooplankton communities at 13 stations within the Guayanilla-Tallaboa Bays complex were examined. Their abundance and community composition were used to examine similarity among stations based on Bray-Curtis dissimilarity index and hierarchical and principal component analysis (PCA). Differences between day and night plankton abundance were also evaluated. The data was used for a detailed evaluation of entrainment potential by EcoElectrica’s cooling water intake system using an iterative pair-wise comparison approach to examine a wide range of scenarios. The results indicate the presence of three homogenous groups of stations with an average zooplankton abundance of 1211, 2641 and 8688 ind/m3. Since Calanoid copepods are a dominant group of species (about 45% of all zooplankton) they were not included during the remaining set of analyses to avoid skewing results. The presence of four homogenous stations was observed using holoplankton (minus calanoids) and meroplankton abundances. In addition, no statistically significant difference (P>0.05) was observed between day and night zooplankton abundance. The use of community composition data and PCA suggests the presence of two large homogenous groups of stations and some a few separate stations related to specific taxa. Of the 91 different entrainment scenarios evaluated, 97% represented total zooplankton and fish eggs entrainment of <1% of the total community for the study area. For fish larvae entrainment, 82% of the scenarios represented <1% of total fish larvae. Estimates for the potential adult fish entrainment was <1% of the total community in 96% of the examined scenarios. Overall, the study area presents a spatially distinct plankton community with a low entrainment potential under the operational conditions examined. Presently, little is known of the stability of those communities and the environmental patterns that covary or modulate such communities.



Introduction

A major goal of section 316(b) of the Clean Water Act (CWA) is to minimize

impingement and entrainment effects by minimizing adverse environmental

impact by requiring the best technology available that considers location,

design, construction and capacity of cooling water intake structures, CWIS,

based on section 301 and 306 of the CWA (USEPA, http://water.epa.gov/

lawsregs/lawsguidance/cwa/316b/ basic.cfm). The effects of CWIS have

been described as impingement and entrainment. While impingement is the

trapping of organisms on the excluding mesh screens of intake systems,

entrainment is the mortality of organisms passing through the mesh and

through the cooling system, induced by mechanical, thermal or chemical

effects in the short and long-term.

Previous work conducted at EcoElectrica have concluded that the

technology used by EcoElectrica on CWIS inlet eliminates impingement of

fishes, marine mammals and seaturtles (Vicente and Associates, 2010;

Otero 2012). These conclusions were derived from direct diver observation

or using underwater video and time-lapse photography that clearly

evidenced how easily fishes swim in close proximity to the screen mesh

without being impinged. Overall, only floating algae and pieces of

seagrasses are being trapped on screens. For that reason, monitoring for

impingement was discontinued.

Entrainment mortality of zooplankton due to the operation of powerplant

cooling water intake systems located in coastal areas has typically been

considered high (ca. 100%) although some reports from other sites have



found low entrainment depending on various conditions, including low

temperature exposure (Mayhew et al 2000). During the 2011 biological

monitoring project program Otero (2012) directly verified the assumption of

high mortality due to entrainment by collecting zooplankton samples on the

cooling water system prior to the outfall; results indicated a decrease in

zooplankton numbers of close to 100% and of phytoplankton of ca. 92%.

Earlier work have found zooplankton abundance in areas close to

EcoElectrica’s pier intake and outfall between >200 to 2,600 ind/m3

(Youngbluth, 1979; García et al., 1995 and García (2012). Otero (2012)

found higher zooplankton abundances ranging 4,000-6,800 ind/m3. These

differences may be attributed to changes in sampling methods, spatial

coverage, and yearly, seasonal and diel changes. Considering the wide

range of abundances observed during previous years, and the limited

spatial coverage of zooplankton sampling during previous works, a closer

look to zooplankton spatial distribution was established as one of the

present goals. Zooplankton sampling with a wider spatial coverage

provides a more robust estimate of the range of zooplankton abundance

found in Guayanilla and Tallaboa Bays complex and will allow for the

examination of a larger range of zooplankton entrainment scenarios based

on data derived within the study area.

The specific objectives of this work are:

1. Evaluate the spatial distribution of zooplankton abundance

throughout Guayanilla Bay;



2. Describe the taxonomic composition of zooplankton in collected

samples;

3. Determine day-night changes of zooplankton in areas associated to

EcoElectrica’s pier including intake and outfall sites;

4. Estimate entrainment effects;

Methods

Study Sites

Thirteen sites, including the intake and outfall sites, were sampled as

depicted in Figure 1. Six of these stations are located adjacent to

EcoElectrica’s pier in order to have better zooplankton community

estimates close to the company’s operation. Other 7 stations were spread

throughout different zones of the Guayanilla-Tallaboa Bays complex

(GTBC) to include a larger variation of the environmental conditions within

the study area in the analysis. The environmental conditions includethe

extremes found in the deep waters outside the bay, a shallow basin to the

northwest of Punta Verraco and the thermal cove south of Costa Sur Power

Plant (CSPP).

Sampling Days and Periods

Sampling was conducted during two separate days, 11 and 12 October

2012. Efforts were focused on night sampling during the first date.

Sampling was conducted on board of EcoElectrica’s work boat from 7:20

PM to 10:05 PM at stations 1, 2, 5, 3, intake (6) and outfall or discharge (7).



These stations include those easily accessible while minimizing sampling

problems in the dark.

Two teams covered all 13 stations during the next day. Each team used a

separate boat, EcoElectrica’s and El Tiburón (Department of Marine

Sciences) from 8:21 AM to 12:30PM. Sampling sheets can be found in

Appendix 1.

Sample Collection

Samples were collected using two standard conical 0.5 m i.d. / 2 m long /

202µm mesh plankton nets each fitted with a calibrated flow meter. The

nets were deployed simultaneously and tows were conducted at each

station at approximately 1m below the surface for a minimum of 5 minutes.

At the outflow station, the net was kept as much as possible within the

plume of the diffuser. After net retrieval, zooplankton was washed down

with the help of a hose fitted with a bilge pump that supplied pressurized

water from the same site. The plankton concentrate was transferred to

wide-mouth jars and fixed immediately with buffered formalin (in seawater)

to a final concentration of 5%.

Evaluation of Plankton Abundance

A Stemple pipette was used to draw two subsamples for each replicate

sample. Each subsample was examined using a dissecting microscope and

average zooplankton and ichthyoplankton abundance was estimated .The



following zooplankton groups were counted as described previously (Otero

2011):

• Holoplankton; permanent drifters (Calanoid, Cyclopoid and

Harpacticoid Copepods; Chaetognatids, Larvaceans, Amphipods,

Sergestoid Shrimps, Forams, and Cumaceans).

• Meroplakton; early ontogenetic (developmental) stages(Decapod

Larvae; Veliger Larvae, Cirriped Larvae, Medusae, Fish Larvae)

Final numbers of individuals per m3 were calculated as follows:

where:

DF = the laboratory dilution factor; FV = Volume filtered in the field= [3.14

X (Net Diameter)² (X) Distance]/4; Distance (m); Net Diameter (m)

Entrainment Estimates

Zooplankton groups were used to assess entrainment effects on

different portions of the community. Different estimates of entrainment

were conducted using plankton counts from different locations of the bay as

representative of the bay populations in conjunction to the findings close to

EcoElectrica’s pier. The use of all possible pair-wise combinations provided

a comprehensive range of entrainment scenarios.



Entrainment was calculated as:

Ezoo = C * Q * M

Where:

Ezoo = zooplankton entrainment (ind per unit time); C = (ind per unit volume) at the intake;

Q = CWIS flow rate (unit volume per day); M = assumed mortality for entrained based on

earlier work (Otero, 2011) = 100%.

The significance of the proportion of entrained plankton (Esig ) is here

defined as the inverse of the proportion of daily plankton intake into

EcoElectrica’s CWIS to the estimate whole Guayanilla Bay plankton

abundance (considering the effects of one tidal volume). That is:

Esig = ;

where:

GBzoo= (Guayanilla Bay volume +tidal volume) *(zooplankton abundance value);

CWISzoo = (daily water used at the CWIS)*(assumed zooplankton abundance at the intake).

The potential effect on adult fish population was calculated assuming one

and two trophic steps from larvae and eggs, respectively (Garcia et al

2012; Otero 2012). However, a series of entrainment effects on adult fish

were calculated using all possible combinations using data from all 13

stations. This provides an examination of the universe of potential

entrainment effects based on the observed variation of zooplankton



community composition within the Guayanilla-Tallaboa Bays complex using

the formula:

where:

%AFE= percent adult fish entrained by EcoElectrica’s operation,

PAFintake= potential adult fish captured by the cooling water system,

PAFbackground= potential adult fish based on Guayanilla Bay static plus tidal volume.

Results and Discussion

Spatial distribution of zooplankton throughout Guayanilla Bay

Variations of total zooplankton abundance at all stations during the day is

shown in Figure 2. A maximum was observed in station 8 (8,688 in/m3),

located in the semi enclosed sub-basin NW of the main basin of Guayanilla

Bay (See Appendix 2 for a detailed data set). The second most abundant

zooplankton community was found at station 1, NW of the intake station, in

close proximity to the adjacent shallow seagrass beds. The minimum

zooplankton abundance was found in station 12 (477 ind/m3), located in the

open ocean waters just outside of Guayanilla Bay. Statistical analysis (SNK

Multiple Comparison ANOVA; Appendix 3) based on total zooplankton

abundance suggests the presence of 3 homogenous overlapping groups of

stations (Fig. 2) averaging 1911, 2641 and 8688 (only station 8). In

comparison, the zooplankton abundance found by Youngbluth (1974)

during the period of December 1973 was ca. 20% of the average found in



the present study. Garcia (2012) reported numbers close to 30% of those

reported here for the period of October 2010. In comparison, the average

total zooplankton abundance during December 2011 (Otero, 2012) was

>2X that found in the present sampling.

Since Calanoid copepods (calanoids) constituted the majority of the total

zooplankton (ca. 45%), they were not included in other statistical analysis

since the group dominated plankton distribution patterns. This is confirmed

by the results of SNK ANOVA of calanoid abundance which separated

station 8 as that with the maximum abundance vs. the rest of the stations

(8X higher abundance; Appendix 3). Statistical analysis for Holoplankton

minus calanoids (Holo-Cal) indicates station 9 (westward of CSPP) with

1390 ind/m3, separated from the other stations with <535 ind/m3 (Figure 3

Top and Appendix 3). The same analysis for meroplankton indicates

additional differences among stations with four overlapping different groups

with average group abundances of 289, 538, 917, 2374 ind/m3 and station

1 containing the highest abundance (Figure 3 Bottom and Appendix 3).

In order to evaluate the similarities among stations, hierarchical cluster

analysis using Bray-Curtis Distance/Similarity matrix and nearest neighbor

clustering was applied to Holo-Cala and meroplankton averages. The

results (Fig 4, Appendix 3) suggest the presence of five groups of stations

corresponding mostly to the covariation of both groups of plankton, with the

exception of station 9, which contained disproportionately high numbers of

holoplankton in relation to meroplankton. The different groups are not

spatially cohesively distributed, as evidenced in Figure 6, probably due to



the complex nature of currents and interconnectivity between different

bottom communities within the study area.

Day-night changes of zooplankton in areas associated to

EcoElectrica’s pier including intake and outfall sites

Day to night comparisons of zooplankton abundance was limited to the six

stations proximal to EcoElectrica’s pier; stations 1, 2, 3, 5, intake (6) and

discharge (7). A graphical comparison of the day and night abundance of

total zooplankton, holoplankton and meroplankton indicates limited

differences between day and night samplings (Figure 5). Statistical

analysis (2-way ANOVA) indicates no differences between day and night,

but there were significant interactions between the period of sampling and

the location variable for mero and holoplankton, that is, larger day/night

differences for holo and meroplankton were observed mostly for station 1

and station 5 (Appendix 3).

Zooplankton community composition

As stated previously, the overall majority of zooplankton found at the

sampling stations were calanoids (approximately 45% of the total

zooplankton community). The next most abundant group of zooplankton,

cirriped larvae, represented 16% of the total while the other 22 taxons

represented 39% (Fig 6). Of the total, ichthyoplankton reached only up to

0.61%, 2/3 of which consisted of fish larvae at various stages of growth.

During BMPP 2011, the proportion of calanoids and cirriped larvae was



very similar (the average of the intake and outfall stations was 46 and 14%,

respectively) to the present abundances while the abundance of

ichthyoplankton was 10 times higher, 6.2% in average. The difference in

relative abundance of ichthyoplankton between both years responds to a

proportional change in abundances of fish to zooplankton and not to an

increase in the abundance of other species.

The abundance of the zooplankton taxons represented in >30% of the

stations was also used to evaluate which of these groups were represented

in stations or group of stations by applying principal component analysis.

The results shown in Figure 7 indicate the presence of two homogeneous

groups. Stations 3, 7(outfall) and 6 (intake) formed one group consistent

with the presence of planulae (coral larvae), chaetognaths, bivalves,

sergestoids and forams (among others). The second group, formed by

stations 12, 2, 4, 5, 13, and 11, was characterized by a more neutral

response to other taxon abundance. Station 9 responded predominantly to

anomuran (crab) larvae, station 10 (Costa Sur Thermal Cove) to both

ascidians and fish larvae (but negatively), station 8 to penaeoids, OPFE,

(Brachyurans and cnidarians) and station 1 to cirripeds and bivalve larvae.

These results suggest the presence of a moderate spatial shift in

zooplankton composition among different locations of the bay that may be

attributed by location specific dynamics.

Estimates of Entrainment on total zooplankton, fish eggs, fish

larvae and potential adult fishes.

The estimates of entrainment were calculated based on the assumption of

a total bay volume of 4.43 x107 m3, a daily tidal volume of 4.3 x 106 m3



(García, 2012 and references within) and a daily CWIS volume of 5.45x104

m3 (as provided by EcoElectrica). The number of combinations of different

estimates was 91 (See Appendix 4 for detailed results). Figures 8-11

present the summary of the entrainment results for total zooplankton, fish

eggs, fish larvae and potential adult fishes. The range of total zooplankton

entrainment was <0.8-2.2%. The cases where entrainment was >1% were

a few, representing 3 out of the 91 possible scenarios. During the 2012

sampling the entrainment potential for fish eggs was mostly below 0.8%.

Only three of the tested scenarios reached values of ca. 1% when using

the fish egg abundance found at station 8 (the maximal fish egg abundance

found during this work) as the intake abundance in combination with those

of stations 9, 13, and 11 as the background concentration. A higher

number of scenarios represented entrainment levels ≥ 1% (16 out of 91) for

fish larvae, reaching a maximum of 4.6%. These results included scenarios

where fish larvae abundances at stations 2, 5, 9, and 12 were used for the

intake abundances versus those at stations 3, 4, 6, 7, 8, 10, 11 and 13 as

representative of Guayanilla Bay overall. It is important to consider that

some of those combinations represent those of stations close to the intake

zone. Although a higher proportion of fish larvae than eggs are potentially

entrained according to the scenarios evaluated, only 4 out of the 91 %AFE

scenarios were ≥ 1. Overall, the scenarios studied support previous

conclusions, >90% of the time the entrainment of total zooplankton, eggs

and potential adult fishes is <1% of the total Guayanilla Bay zooplankton.

Although our results indicate that ca. 18% of the scenarios result in >1%

entrainment of the total bay larvae, the combination of abundances that

produce these estimates is due to large differences in larval abundance

(high patchiness) among different stations. This higher entrainment



estimates are obtained when intake abundances are about 8X higher than

those assumed to be representative of Guayanilla Bay.

Conclusions

The abundance of total zooplankton pooled stations into 3 homogenous

“groups” averaging 1,211, 2,641 and 8,688 ind/m3. This last average is

attributed to one station (8), which is located within the western Guayanilla

Bay sub basin. This basin is spatially associated with the inputs of rivers

and creeks, to shallow depths and limited mixing with the rest of the

Guayanilla Bay. This explains the increase in standing stock of plankton at

this site.

Calanoid copepods (calanoids) were about 45% of the total zooplankton

population, in accordance to previous work. This is not surprising as

worldwide they are the most abundant form of zooplankton (Johnson and

Allen, 2005).

Using the abundance of holoplankton (minus calanoids) and meroplankton

abundances, 4 groups of stations were differentiated (st’s 12 and 10; st’s 2,

4, 5, 8; stations 3, 7, 6, 11, 13; st 1). These groups are not grouped

according to spatial proximity, due probably to interaction of currents,

nutrients and biotic factors (i.e. productivity, species to species

differences).

Overall, no significant differences in total, holoplankton (minus calanoids)

and meroplankton counts were observed between day and night periods.

However, some differences were found at stations 1 and 5. This lack of



statistical difference is attributed in part to natural patchiness. The role that

vertical migration and water currents have at the different sites and their

relationship with the relative day/night stability in zooplankton abundance

found during this survey remains unclear. This may respond to the fact that

different groups show different patterns of vertical migration as adaptive

strategies. Normally, zooplankton must ascend in the evening and descend

at dawn but reverse migrations can sometimes be logically explained for

different groups as a strategy to escape predators. For example, the

reverse migration of the small Pseudocalanus can be explained as an

escape strategy from the 'normally' migrating large invertebrate predator

Calanus, that in turn is affected by fish predation (Lampert ,1989).

Calanoid copepods and cirriped larvae are in general the most abundant

taxa, confirming results from previous years. The proportional abundance

of ichthyoplankton was 10X lower during 2012 in comparison to 2011,

suggesting a large inter-annual variation.

Two main homogenous groups of stations were found using PCA

consisting of stations 2, outfall, and intake (associated with the abundance

of planuelae, chaetognaths, bivalves, sergestoids and forams) and stations

12, 2, 4, 5, 13, and 11, characterized less strongly to a larger array of

taxons. The other stations formed dissimilar identities based strongly to

specific plankton taxa; station 9 was associated to anomuran crab larvae

(i.e. hermits and mole crabs, among others), station 8 to peneaoids

(shrimps) and station 10 inversely related to ascidians and fish larvae.

Thus, the present data suggests that contrary to harboring a homogenous

plankton community, the Guayanilla-Tallaboa Bay complex support a



spatially distinct plankton community. The stability of such communities is

unknown.

Overall, the entrainment results confirm previous work suggesting that the

significance of entrainment due to the operation of EcoElelctrica’s CWIS is

low. Total zooplankton and fish egg entrainment is <1% in 88 of 91

scenarios tested. Although the potential entrainment of fish larvae was 1-

5% of the total bay plankton in 18% of all scenarios tested, the impact in

the potential adult fish (PAF) population was low, since %AFE was >1 only

in 4 of the scenarios tested.

It is observed that under the present operational conditions, entrainment

rates close to 1% could be obtained only when zooplankton abundances in

the proximity of the intake is 8X higher than the general Guayanilla Bay

zooplankton abundance. This condition is infrequent according to present

and past observations.



References

García, J. R., E. Ojeda and A. González. 1995. Zooplankton/ichthyo-plankton communities of Guayanilla and Tallaboa Bays: Taxonomic structure and spatial/temporal patterns. Report submitted to Gramatges and Associates. EcoEléctrica Power Plant Studies. San Juan, P. R. December, 1995. 91 p.

Garcia, J.R. 2012. Analysis of Zooplankton Entrainment by the EcoElectrica LNG Power Plant in Guayanilla Bay, P. R. during October, 2010

Johnson, W.S. and D.M. Allen. 2005. Zooplankton of the Atlantic and Gulf Coasts. A Guide to Their Identification and Ecology. Johns Hpkins University Press. Baltimore. 379pp.

Lampert, W. 1989. The adaptive significance of diel vertical migration of zooplankton. Functional Ecology, 3, 21-27.

Mayhew, D.A., L.D. Jensen, D.F. Hanson and P.H. Muessig. 2000. A comparative review of entrainment survival studies at power plants in estuarine environments. 3 (Sup 1): 295-301.

Otero, E. 2012. Impingement and Entrainment by EcoEléctrica LNG Power Plant, Guayanilla, Puerto Rico: December 2011.

PRNC #179. 1971-1974 Puerto Rico Nuclear Center Guayanilla Bay Environmental Report. 157pp.

Vicente and Associates, 2010. Biological Monitoring Program Plan Implementation: 2005-2008. Submitted to EcoElectrica. 2010.

Youngbluth, M. 1974. Zooplankton 1971-1974, Guayanilla Bay

Environmental Report. PRNC-179. Puerto Rico Nuclear Center.



Figures



Figure 1. Zooplankton sampling sites (13). The blue square contains sites sampled during the

night of 11 Oct 2012. North is towards the top of the figure.



Figure 2. Average total zooplankton abundance divided in holo and meroplankton. Samples

are arranged in order of total abundance

.



Figure 3. Abundance of Holoplankton minus calanoids (Top) and and Meroplankton

(Bottom). Error bars represent 1 SD.



Figure 4.Summary results from hierarchical cluster analysis of Holoplankton-minus calanoids

using Bray-Curtis Distances and Nearest Neighbor Clustering (segmented polygon)

over the representation of holoplankton minus calnoids vs meroplankton average

abundance.



Figure 5. Average total, holo- and meroplankton abundance for stations sampled during night

and day. A two way analysis of variance indicated no overall statistically significant

differences between day and night samples at these stations. However, the

interaction term was significant thus such difference is dependent on stations.

Holoplankton increased during the night at station 1 and 5 while meroplankton

increase was mainly observed at station 5.



Figure 6. Percent composition of the 24 zooplankton groups found in > 30% of the stations.

The abundance used as the base for calculation is 2374 ind m-3 ; H= holoplankton; M=

meroplankton.



Figure 7. Principal component analysis using zooplankton groups present in >30% of all

stations. Red dots represent the strength of each station along the axes of the first 2

PC while lines represent the relative strength of each group of plankton along the PC

gradient. Lines towards one of the stations symbol indicate plankton groups more

strongly associated to that station.



Figure 8. Frequency distribution of the proportion of entrained zooplankton to that of the

total Guayanilla Bay community. The numbers over the bars are the proportions for

each % entrainment range.



Figure 9. Frequency distribution of the proportion of entrained fish eggs to that of the total


entrainment range.



Figure 10. Frequency distribution of the proportion of entrained fish larvae to that of the total


entrainment range.



Figure 11. Frequency distribution of the proportion of entrained potential adult fishes to that

of the total Guayanilla Bay community. The numbers over the bars are the proportions

for each % entrainment range.



Apendices



Appendix 1. Sampling Sheets

BMPP EcoElectrica 2012# de Red

Date Time Station Sample ID Before After 1, 2, 3 o 411-Oct-12 7:20pm 1 #1AN 0 8911 1 11-Oct-12 7:20pm 1 #1BN 95 8098 3

11-Oct-12 7:54pm 2 #2AN 8942 13909 1 11-Oct-12 7:54pm 2 #2BN 8090 12505 3

11-Oct-12 8:26pm 5 #5AN 13909 18320 1 11-Oct-12 8:26pm 5 #5BN 12505 16478 3

People on Board Boat NameAurea Rodriguez Ronald

AnotacionesDuane Damaris

Alvin ErnestoNotes:

Sample Designation ECO12SSREPXTT Target NET Reading (3-4 Thousands)where SS= station number; X= A or B; TT= Dia o Noc

Page number= ___1 ________ of _____3______

Reading Flow Meter




Date Time Station Sample ID Before After 1, 2, 3 o 411-Oct-12 8:51pm 3 #3AN 18320 24343 111-Oct-12 8:51pm 3 #3BN 16478 23043 3

11-Oct-12 9:17pm intake #intakeAN 24343 29994 111-Oct-12 9:17pm intake #intakeBN 23043 27683 3

11-Oct-12 9:42pm discharge #dischargeAN 29994 34132 111-Oct-12 9:42pm discharge #dischargeBN 27683 31364 3

People on Board Boat NameAurea Ronald


Alvin ErnestoNotes:


Page number= _____2 ______ of ______3____

Reading Flow Meter


Date Time Station Sample ID Before After 1, 2, 3 o 411-Oct-12 10:05pm discharge #dischargeOHAN 34132 38304 111-Oct-12 10:05pm discharge #dischargeOHBN 31364 36012 3

People on Board Boat NameAurea Ronald


Alvin ErnestoNotes:


Page number= ______3 _____ of ______3_____

Reading Flow Meter




Date Time Station Sample ID Before After 1, 2, 3 o 412-Oct-12 9:26am Intake Intake AD 55208 60719 112-Oct-12 9:26am Intake Intake BD 50947 56521 3

12-Oct-12 9:49am 4 #4AD 60719 65596 112-Oct-12 9:49am 4 #4BD 56521 60494 3

12-Oct-12 10:26am 3 #3AD 65596 71107 112-Oct-12 10:26am 3 #3BD 60494 65303 3

People on Board Boat NameAurea E. Rodríguez Alvin Ortiz: capitan

AnotacionesFabian Chaparro Ronald Martinez

Derek SotoNotes:


Page number= ______2 _____ of _____3______

Reading Flow Meter


Date Time Station Sample ID Before After 1, 2, 3 o 412-Oct-12 10:48am 1 #1AD 71107 75454 112-Oct-12 10:48am 1 #1BD 65303 70206 3

12-Oct-12 11:44am 13 #13AD 75454 80204 112-Oct-12 11:44am 13 #13BD 70206 74839 3

People on Board Boat NameAurea E. Rodriguez Alvin Ortiz: capitan

AnotacionesFabian Chaparro Ronald Martinez

Derek SotoNotes:


Page number= ______3 _____ of ____3_______

Reading Flow Meter





12-Oct-12 10:06am 11 #11AD 105427 113475 212-Oct-12 10:06am 11 #11BD 6358 14846 4

12-Oct-12 10:37am 8 #8AD 113475 118675 212-Oct-12 10:37am 8 #8BD 14846 19440 4

People on Board Boat NameGaspar Brenda

AnotacionesLiajay Ernesto Se dano el motor durante la corrida 8 a las 10:37am

Notes:


Page number= _____1 ______ of _____2_______

Reading Flow Meter



12-Oct-12 11:51am 9 #9AD 124561 125499 212-Oct-12 11:51am 9 #9BD 27442 33166 4

12-Oct-12 12:28pm 10 #10AD 125499 129980 212-Oct-12 12:28pm 10 #10BD 33166 38780 4

People on Board Boat NameGaspar Brenda

AnotacionesLiajay Ernesto

Notes:


Page number= ______2 _____ of ____________of 2

Reading Flow Meter



Appendix 2. Zooplankton Counts



UPRM - Marine Sciences BMPP - EcoElectrica Guayanilla

Station: 1 Mesh:

Date: October 12, 2012 Time: 10:48

Replicate: Filtered Volume (m3): 22.93

Field Sample Code: #1 A Day

HOLOPLANKTON TAXA Dil. Factor Aliquot 1 Aliquot 2 Mean#

Ind/Sample Ind/m 3

Calanoid Copepods 200 144 193 168.50 33700 1469.69Cyclopoid Copepods 200 12 8 10.00 2000 87.22Harpacticoid Copepods 200 9 6 7.50 1500 65.42Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 200 12 13 12.50 2500 109.03Larvaceans 200 31 21 26.00 5200 226.78Cnidarians 200 1 0 0.50 100 4.36SyphonophoresAmphipodsSergestoid Shrimps IsopodsPycnogonidsCumaceansOstracodsForaminiferans 200 4 0 2.00 400 17.44CtenophoresMysidsBryozoans (colonies)Cladocera 200 8 14 11.00 2200 95.94Unidentified

Total Holoplankton 2075.88

MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid Caridean Anomuran Macruran Brachyuran 200 1 1 1.00 200 8.72Decapod Eggs 200 1 1 1.00 200 8.72

Veliger Larvae 200 86 98 92.00 18400 802.44Bivalve larvaePolychaete Larvae 200 9 2 5.50 1100 47.97Cirriped Larvae 200 221 190 205.50 41100 1792.41Ascidean LarvaeStomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 200 2 4 3.00 600 26.17Tanaidaceans 200Planula 200 1 0 0.50 100 4.36Unidentified

Fish Eggs Round 200 1 2 1.50 300 13.08 Pointed

Fish Larvae (Unknown)

Total Meroplankton 2703.88

TOTAL ZOOPLANKTON 4779.76



UPRM - Marine Sciences BMPP - EcoElectrica Guayanilla

Station: 1 Mesh: 202

Date: October 12, 2012 Time: 10:48 AM

Replicate: B Filtered Volume (m3): 25.86

Field Sample Code: 1B-Day

HOLOPLANKTON TAXA Dil. Factor Aliquot 1 Aliquot 2 Mean # Ind/Sample Ind/m 3

Calanoid Copepods 200 75 103 89.00 17800 688.32Cyclopoid Copepods 200 10 20 15.00 3000 116.01Harpacticoid Copepods 200 5 5 5.00 1000 38.67Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 200 8 7 7.50 1500 58.00Larvaceans 200 16 10 13.00 2600 100.54Cnidarians 200 1 2 1.50 300 11.60Syphonophores 0.00AmphipodsSergestoid Shrimps (protozoea) 200 13 11 12.00 2400 92.81IsopodsPycnogonidsCumaceansOstracodsForaminiferansCtenophoresMysidsBryozoans (colonies) 200 1 0 0.50 100 3.87Cladocera 200 6 5 5.50 1100 42.54Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid Caridean Anomuran 200 2 0 1.00 200 7.73 Macruran Brachyuran 200 0 1 0.50 100 3.87Decapod Eggs

Veliger Larvae 200 15 16 15.50 3100 119.88Bivalve larvae 200 104 90 97.00 19400 750.19Polychaete Larvae 200 3 3 3.00 600 23.20Cirriped Larvae 200 155 135 145.00 29000 1121.42Ascidean LarvaeStomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea )Tanaidaceans

Fish Eggs Round 200 1 0 0.50 100 3.87 Oval 200 1 1 1.00 200 7.73

Fish Larvae (Unknown) 200 2 0 1.00 200 7.73





UPRM - Marine Sciences

Station: 2 Mesh:



Field Sample Code: #2 A D


Ind/Sample Ind/m 3

Calanoid Copepods 50 534 548 541.00 27050 661.69Cyclopoid Copepods 50 6 2 4.00 200 4.89Harpacticoid Copepods 50 77 76 76.50 3825 93.57Monstrilloid CopepodCaligoid CopepodCopepod Nauplii 50 1 1 1.00 50 1.22Chaetognath Worms 50 102 104 103.00 5150 125.98Larvaceans 50 3 3 3.00 150 3.67CnidariansSyphonophoresAmphipodsSergestoid Shrimps (mainly adults)Isopods 50 0 1 0.50 25 0.61PycnogonidsCumaceansOstracods 50Foraminiferans 50 1 0 0.50 25 0.61CtenophoresMysidsBryozoans (colonies)Cladocera 50 21 22 21.50 1075 26.30Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid 50 1 0 0.50 25 0.61 Caridean 50 13 12 12.50 625 15.29 Anomuran 50 Macruran Brachyuran 4 2 3.00 0 0.00Decapod Eggs 50 10 3 6.50 325 7.95

50Veliger Larvae 50 141 239 190.00 9500 232.39Bivalve larvae 50Polychaete Larvae 50 6 12 9.00 450 11.01Cirriped Larvae 50 29 48 38.50 1925 47.09Ascidean Larvae 50 3 1 2.00 100 2.45Stomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 50 1 4 2.50 125 3.06Tanaidaceans 50Planula 50 1 2 1.50 75 1.83Unidentified












Calanoid Copepods 100 189 200 194.50 19450 574.59Cyclopoid Copepods 100 49 38 43.50 4350 128.51Harpacticoid Copepods 100 18 15 16.50 1650 48.74Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 100 55 56 55.50 5550 163.96Larvaceans 100 3 0 1.50 150 4.43Cnidarians 100 5 4 4.50 450 13.29Syphonophores 100 0 1 0.50 50 1.48AmphipodsSergestoid Shrimps (protozea) 100 3 3 3.00 300 8.86Isopods 100 1 2 1.50 150 4.43PycnogonidsCumaceansOstracodsForaminiferans 100 1 0 0.50 50 1.48CtenophoresMysidsBryozoans (cyphonautes) 100 1 0 0.50 50 1.48Cladocera 100 84 90 87.00 8700 257.02Unidentified 0.00


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid Caridean 100 8 10 9.00 900 26.59 Anomuran Macruran Brachyuran 100 5 10 7.50 750 22.16Decapod Eggs 100 1 1 1.00 100 2.95

Veliger Larvae 100 31 55 43.00 4300 127.03Bivalve larvae 100 23 21 22.00 2200 64.99Polychaete Larvae 100 2 4 3.00 300 8.86Cirriped Larvae 100 24 21 22.50 2250 66.47Ascidean Larvae 100 1 0 0.50 50 1.48Stomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea )Tanaidaceans

Unidentified







Station: 3 Mesh:





Ind/Sample Ind/m 3

Calanoid Copepods 100 333 364 348.50 34850 1199.24Cyclopoid Copepods 100 15 13 14.00 1400 48.18Harpacticoid Copepods 100 11 17 14.00 1400 48.18Monstrilloid CopepodCaligoid CopepodCopepod Nauplii 100 0 1 0.50 50 1.72Chaetognath Worms 100 3 3 3.00 300 10.32Larvaceans 100 14 9 11.50 1150 39.57Cnidarians 100 1 3 2.00 200 6.88SyphonophoresAmphipodsSergestoid Shrimps (mainly adults) 100 2 1 1.50 150 5.16IsopodsPycnogonidsCumaceansOstracodsForaminiferans 100 1 2 1.50 150 5.16CtenophoresMysidsBryozoans (colonies)Cladocera 100 12 20 16.00 1600 55.06Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid Caridean Anomuran Macruran BrachyuranDecapod Eggs 100 2 1 1.50 150 5.16

100Veliger Larvae 34 46 40.00 0 0.00Bivalve larvaePolychaete Larvae 100 4 5 4.50 450 15.49Cirriped Larvae 100 95 89 92.00 9200 316.59Ascidean Larvae 100 0 1 0.50 50 1.72Stomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 100 13 10 11.50 1150 39.57Tanaidaceans

Unidentified












Calanoid Copepods 200 168 150 159.00 31800 1253.94Cyclopoid Copepods 200 15 18 16.50 3300 130.13Harpacticoid Copepods 200 8 10 9.00 1800 70.98Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 200 5 4 4.50 900 35.49Larvaceans 200 14 12 13.00 2600 102.52Cnidarians 200 2 1 1.50 300 11.83SyphonophoresAmphipodsSergestoid Shrimps (protozea) 200 15 17 16.00 3200 126.18IsopodsPycnogonidsCumaceansOstracodsForaminiferans 200 2 0 1.00 200 7.89CtenophoresMysidsBryozoans (cyphonautes)Cladocera 200 10 8 9.00 1800 70.98Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid Caridean Anomuran Macruran BrachyuranDecapod Eggs 200 1 0 0.50 100 3.94

Veliger Larvae 200 10 14 12.00 2400 94.64Bivalve larvae 200 28 32 30.00 6000 236.59Polychaete Larvae 200 3 4 3.50 700 27.60Cirriped Larvae 200 75 70 72.50 14500 571.77Ascidean LarvaeStomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 200 3 4 3.50 700 27.60Tanaidaceans

Unidentified

Fish Eggs Round Pointed






Station: 4 Mesh:





Ind/Sample Ind/m 3

Calanoid Copepods 50 309 278 293.50 14675 570.57Cyclopoid Copepods 50 20 15 17.50 875 34.02Harpacticoid Copepods 50 19 18 18.50 925 35.96Monstrilloid Copepod 50Caligoid Copepod 50Copepod Nauplii 50Chaetognath Worms 50 5 6 5.50 275 10.69Larvaceans 50 10 3 6.50 325 12.64Cnidarians 50 2 1 1.50 75 2.92Syphonophores 50Amphipods 50Sergestoid Shrimps (mainly adults) 50 2 6 4.00 200 7.78Isopods 50Pycnogonids 50Cumaceans 50Ostracods 50Foraminiferans 50Ctenophores 50Mysids 50Bryozoans (colonies) 50Cladocera 50 37 29 33.00 1650 64.15Unidentified 50


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid 50 Caridean 50 0 2 1.00 50 1.94 Anomuran 50 Macruran 50 Brachyuran 50Decapod Eggs 50 7 4 5.50 275 10.69

50Veliger Larvae 50 119 98 108.50 5425 210.93Bivalve larvae 50Polychaete Larvae 50 1 0 0.50 25 0.97Cirriped Larvae 50 18 22 20.00 1000 38.88Ascidean Larvae 50 1 1 1.00 50 1.94Stomatopod Larvae 50Actinotrochs 50Echinoderm (Ophiuroidea ) 50 5 2 3.50 175 6.80Tanaidaceans 50

50Unidentified 50

50Fish Eggs 50 Round 50 10 10 10.00 500 19.44 Pointed 50

50Fish Larvae (Unknown) 50 3 4 3.50 175 6.80









Calanoid Copepods 80 121 88 104.50 8360 399.05Cyclopoid Copepods 80 27 30 28.50 2280 108.83Harpacticoid Copepods 80 8 5 6.50 520 24.82Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 80 11 5 8.00 640 30.55Larvaceans 80 7 13 10.00 800 38.19Cnidarians 80 2 2 2.00 160 7.64SyphonophoresAmphipodsSergestoid Shrimps (protozea) 80 35 20 27.50 2200 105.01IsopodsPycnogonidsCumaceansOstracodsForaminiferansCtenophoresMysidsBryozoans (colony) 80 1 0 0.50 40 1.91Cladocera 80 7 13 10.00 800 38.19Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid 80 2 0 1.00 80 3.82 Caridean 80 1 0 0.50 40 1.91 Anomuran Macruran Brachyuran 80 3 0 1.50 120 5.73Decapod Eggs

Veliger Larvae 80 8 10 9.00 720 34.37Bivalve larvae 80 52 62 57.00 4560 217.66Polychaete LarvaeCirriped Larvae 80 18 12 15.00 1200 57.28Ascidean Larvae 80 2 1Stomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 80 2 3 2.50 200 9.55Tanaidaceans

Unidentified







Station: 5 Mesh:





Calanoid Copepods 50 113 129 121.00 6050 253.24Cyclopoid Copepods 50 9 11 10.00 500 20.93Harpacticoid Copepods 50 43 37 40.00 2000 83.72Monstrilloid CopepodCaligoid CopepodCopepod Nauplii 50 1 0 0.50 25 1.05Chaetognath Worms 50 2 5 3.50 175 7.33Larvaceans 50 8 11 9.50 475 19.88Cnidarians 50 1 0 0.50 25 1.05SyphonophoresAmphipodsSergestoid Shrimps (mainly adults)IsopodsPycnogonidsCumaceansOstracodsForaminiferansCtenophoresMysidsBryozoans (colonies)Cladocera 50 21 16 18.50 925 38.72Unidentified 50



Veliger Larvae 50 66 92 79.00 3950 165.34Bivalve larvaePolychaete LarvaeCirriped Larvae 50 18 19 18.50 925 38.72Ascidean Larvae 50 0 1 0.50 25 1.05Stomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 50 5 4 4.50 225 9.42Tanaidaceans 50

UnidentifiedEchinoderm (Bipinnaria) 50 1 0 0.50 25 1.05Fish Eggs Round 50 10 11 10.50 525 21.98 Pointed 50 2 1 1.50 75 3.14






Station: 5 Mesh:



Field Sample Code: 5B - D


Calanoid Copepods 100 110 108 109.00 10900 463.83Cyclopoid Copepods 100 18 21 19.50 1950 82.98Harpacticoid Copepods 100 21 28 24.50 2450 104.26Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 100 10 8 9.00 900 38.30Larvaceans 100 15 17 16.00 1600 68.09Cnidarians 100 1 0 0.50 50 2.13SyphonophoresAmphipodsSergestoid Shrimps 100 15 22 18.50 1850 78.72IsopodsPycnogonidsCumaceansOstracodsForaminiferans 100 1 0 0.50 50 2.13CtenophoresMysidsBryozoans (colonies) 100 1 0 0.50 50 2.13Cladocera 100 28 31 29.50 2950 125.53Unidentified



Veliger Larvae 100 52 62 57.00 5700 242.55Bivalve larvae 100 15 19Polychaete LarvaeCirriped Larvae 100 30 43 36.50 3650 155.32Ascidean Larvae 100 1 0Stomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 100 3 5 4.00 400 17.02Tanaidaceans

UnidentifiedEchinoderm (Bipinnaria)Fish Eggs Round 100 5 12 8.50 850 36.17 Pointed






Station: 8 Mesh:





Ind/Sample Ind/m 3

Calanoid Copepods 400 737 754 745.50 298200 9606.41Cyclopoid Copepods 400 3 0 1.50 600 19.33Harpacticoid Copepods 400 2 0 1.00 400 12.89Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 400 3 2 2.50 1000 32.22Larvaceans 400 12 10 11.00 4400 141.75Cnidarians 400 4 0 2.00 800 25.77SyphonophoresAmphipodsSergestoid Shrimps (mainly adults)IsopodsPycnogonidsCumaceansOstracodsForaminiferansCtenophoresMysidsBryozoans (colonies)Cladocera 400 1 14 7.50 3000 96.65Unidentified



Veliger Larvae 400 0 2 1.00 400 12.89Bivalve larvae 400Polychaete Larvae 400 3 5 4.00 1600 51.55Cirriped Larvae 400 7 6 6.50 2600 83.76Ascidean LarvaeStomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 400 2 0 1.00 400 12.89Tanaidaceans

Unidentified







Station: 8 Mesh:



Field Sample Code: 8 B - D


Calanoid Copepods 400 635 689 662.00 264800 6274.70Cyclopoid Copepods 400 10 5 7.50 3000 71.09Harpacticoid Copepods 400 2 0 1.00 400 12.89Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 400 4 2 3.00 1200 38.66Larvaceans 400 15 12 13.50 5400 173.97Cnidarians 400 3 0 1.50 600 19.33SyphonophoresAmphipodsSergestoid Shrimps IsopodsPycnogonidsCumaceansOstracodsForaminiferansCtenophoresMysidsBryozoans (colonies)Cladocera 400 10 8 9.00 3600 115.98Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae 400 Penaeoid 400 1 3 2.00 800 25.77 Caridean 400 1 0 0.50 200 6.44 Anomuran Macruran Brachyuran 400 4 3 3.50 1400 45.10Decapod Eggs

Veliger Larvae 400 5 6 5.50 2200 70.88Bivalve larvae 400 1 0Polychaete Larvae 400 4 3 3.50 1400 45.10Cirriped Larvae 400 10 15 12.50 5000 161.08Ascidean LarvaeStomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 400 0 2 1.00 400 12.89Tanaidaceans

Unidentified







Station: 9 Mesh:





Ind/Sample Ind/m 3

Calanoid Copepods 60 162 175 168.50 10110 361.07Cyclopoid Copepods 60 2 5 3.50 210 7.50Harpacticoid Copepods 60 9 16 12.50 750 26.79Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 60 6 10 8.00 480 17.14Larvaceans 180 264 283 273.50 49230 1758.21Cnidarians 60 3 4 3.50 210 7.50SyphonophoresAmphipodsSergestoid Shrimps (mainly adults) 60 2 4 3.00 180 6.43IsopodsPycnogonidsCumaceansOstracodsForaminiferansCtenophoresMysidsBryozoans (colonies)Cladocera 60 17 25 21.00 1260 45.00Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid Caridean 60 9 10 9.50 570 20.36 Anomuran 60 28 35 31.50 1890 67.50 Macruran Brachyuran 60 3 4 3.50 210 7.50Decapod Eggs 60 2 5 3.50 210 7.50

Veliger Larvae 60 3 12 7.50 450 16.07Bivalve larvaePolychaete Larvae 60 3 5 4.00 240 8.57Cirriped Larvae 60 65 73 69.00 4140 147.86Ascidean Larvae 60 2 4 3.00 180 6.43Stomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 60 6 10 8.00 480 17.14Tanaidaceans

Unidentified







Station: 9 Mesh:



Field Sample Code: 9B - D


Calanoid Copepods 80 180 188 184.00 14720 487.58Cyclopoid Copepods 80 5 7 6.00 480 15.90Harpacticoid Copepods 80 7 10 8.50 680 22.52Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 80 7 5 6.00 480 15.90Larvaceans 100 227 250 238.50 23850 790.00Cnidarians 80 2 4 3.00 240 7.95SyphonophoresAmphipodsSergestoid Shrimps (mainly adults) 80 4 3 3.50 280 9.27IsopodsPycnogonidsCumaceansOstracodsForaminiferansCtenophoresMysidsBryozoans (colonies)Cladocera 80 15 18 16.50 1320 43.72Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid Caridean 80 12 8 10.00 800 26.50 Anomuran 80 27 30 28.50 2280 75.52 Macruran Brachyuran 80 3 4 3.50 280 9.27Decapod Eggs 80 2 5 3.50 280 9.27

Veliger Larvae 80 3 12 7.50 600 19.87Bivalve larvae 80 2 1 1.50 120 3.97Polychaete Larvae 80 3 5 4.00 320 10.60Cirriped Larvae 80 65 73 69.00 5520 182.84Ascidean Larvae 80 2 4 3.00 240 7.95Stomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 80 6 10 8.00 640 21.20Tanaidaceans

Unidentified







Station: 10 Mesh:

Date: October 12, 2012 Time: 12:28 PM




Ind/Sample Ind/m 3

Calanoid Copepods 100 104 114 109.00 10900 461.24Cyclopoid Copepods 100 7 15 11.00 1100 46.55Harpacticoid Copepods 100 20 19 19.50 1950 82.52Monstrilloid CopepodCaligoid CopepodCopepod Nauplii 100 0 2 1.00 100 4.23Chaetognath Worms 100 2 0 1.00 100 4.23LarvaceansCnidariansSyphonophoresAmphipods 100 0 1 0.50 50 2.12Sergestoid ShrimpsIsopodsPycnogonidsCumaceansOstracodsForaminiferansCtenophoresMysidsBryozoans (colonies) 100 1 0 0.50 50 2.12Cladocera 100 3 3 3.00 300 12.70Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid Caridean 100 9 4 6.50 650 27.51 Anomuran Macruran Brachyuran 100 2 3 2.50 250 10.58Decapod Eggs

Veliger LarvaeBivalve larvaePolychaete Larvae 100 1 2 1.50 150 6.35Cirriped Larvae 100 7 20 13.50 1350 57.13Ascidean Larvae 100 1 4 2.50 250 10.58Stomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea )Tanaidaceans

Unidentified







Station: 10 Mesh:



Field Sample Code: 10 B-D


Calanoid Copepods 100 89 103 96.00 9600 324.24Cyclopoid Copepods 100 15 18 16.50 1650 55.72Harpacticoid Copepods 100 15 18 16.50 1650 55.72Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 100 2 1 1.50 150 5.07LarvaceansCnidariansSyphonophoresAmphipods 100 1 0 0.50 50 1.69Sergestoid ShrimpsIsopodsPycnogonidsCumaceansOstracodsForaminiferansCtenophoresMysidsBryozoans (colonies)Cladocera 100 5 3 4.00 400 13.51Unidentified



Veliger LarvaeBivalve larvae 100 2 0 1.00 100 3.38Polychaete Larvae 100 1 2 1.50 150 5.07Cirriped Larvae 100 22 16 19.00 1900 64.17Ascidean Larvae 100 2 3 2.50 250 8.44Stomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea )Tanaidaceans

Unidentified







Station: 12 Mesh:


Replicate: Filtered Volume (m3): 30



Calanoid Copepods 65 116 115 115.50 7508 250.25Cyclopoid Copepods 65 5 5 5.00 325 10.83Harpacticoid Copepods 65 25 24 24.50 1592.5 53.08Monstrilloid Copepod 65Caligoid Copepod 65Copepod Nauplii 65Chaetognath Worms 65 16 8 12.00 780 26.00Larvaceans 65 12 7 9.50 617.5 20.58Cnidarians 65Syphonophores 65Amphipods 65Sergestoid Shrimps (mainly adults) 65 0 2 1.00 65 2.17Isopods 65Pycnogonids 65Cumaceans 65Ostracods 65Foraminiferans 65 3 0 1.50 97.5 3.25Ctenophores 65Mysids 65Bryozoans (colonies) 65Cladocera 65 25 27 26.00 1690 56.33Unidentified 65


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid 65 1 1 1.00 65 2.17 Caridean 65 8 6 7.00 455 15.17 Anomuran 65 Macruran 65 Brachyuran 65 3 3 3.00 195 6.50Decapod Eggs 65 4 3 3.50 227.5 7.58

Veliger Larvae 65 27 23 25.00 1625 54.17Bivalve larvae 65Polychaete Larvae 65 1 0 0.50 32.5 1.08Cirriped Larvae 65 2 0 1.00 65 2.17Ascidean Larvae 65Stomatopod Larvae 65Actinotrochs 65Echinoderm (Ophiuroidea ) 65 0 1 0.50 32.5 1.08Tanaidaceans 65Planula 65 0 1 0.50 32.5 1.08Unidentified 65 2 0 1.00 65 2.17

Fish Eggs 65 Round 65 2 2 2.00 130 4.33 Pointed 65 1 0 0.50 32.5 1.08

Fish Larvae (Unknown) 65 4 3 3.50 227.5 7.58





Station: 12 Mesh:



Field Sample Code: 12 B-D


Calanoid Copepods 60 108 115 111.50 6690 200.72Cyclopoid Copepods 60 8 9 8.50 510 15.30Harpacticoid Copepods 60 19 23 21.00 1260 37.80Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 60 13 10 11.50 690 20.70Larvaceans 60 7 9 8.00 480 14.40Cnidarians 60 1 0SyphonophoresAmphipodsSergestoid Shrimps (mainly adults) 60 3 0 1.50 90 2.70IsopodsPycnogonidsCumaceansOstracodsForaminiferans 60 2 0 1.00 60 1.80CtenophoresMysidsBryozoans (colonies)Cladocera 60 35 26 30.50 1830 54.91Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid Caridean 60 7 5 6.00 360 10.80 Anomuran 60 2 0 Macruran Brachyuran 60 5 4 4.50 270 8.10Decapod Eggs 60 7 5 6.00 360 10.80

Veliger Larvae 60 16 14 15.00 900 27.00Bivalve larvae 60 5 3 4.00 240 7.20Polychaete Larvae 60 1 0 0.50 30 0.90Cirriped Larvae 60 3 2 2.50 150 4.50Ascidean LarvaeStomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 60 1 0 0.50 30 0.90TanaidaceansPlanulaUnidentified







Station: 13 Mesh:





Ind/Sample Ind/m 3

Calanoid Copepods 50 166 194 180.00 9000 359.27Cyclopoid Copepods 50 3 2 2.50 125 4.99Harpacticoid Copepods 50 11 15 13.00 650 25.95Monstrilloid CopepodCaligoid CopepodCopepod Nauplii 50 1 0 0.50 25 1.00Chaetognath Worms 50 2 1 1.50 75 2.99Larvaceans 50 39 33 36.00 1800 71.86Cnidarians 50 2 1 1.50 75 2.99SyphonophoresAmphipodsSergestoid Shrimps (mainly adults)IsopodsPycnogonidsCumaceansOstracodsForaminiferans 50 1 1 1.00 50 2.00CtenophoresMysidsBryozoans (colonies)Cladocera 50 32 40 36.00 1800 71.86Unidentified



Veliger Larvae 50 255 264 259.50 12975 517.96Bivalve larvaePolychaete Larvae 50 2 4 3.00 150 5.99Cirriped Larvae 50 200 156 178.00 8900 355.29Ascidean Larvae 50 1 4 2.50 125 4.99Stomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 50 14 14 14.00 700 27.94Tanaidaceans

Unidentified

Fish Eggs Round 50 3 2 2.50 125 4.99 Pointed 50 1 0 0.50 25 1.00






Station: 13 Mesh:



Field Sample Code: 13 B -D


Calanoid Copepods 60 153 166 159.50 9570 391.67Cyclopoid Copepods 60 5 8 6.50 390 15.96Harpacticoid Copepods 60 10 13 11.50 690 28.24Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 60 5 2 3.50 210 8.60Larvaceans 60 40 42 41.00 2460 100.70Cnidarians 60 1 3 2.00 120 4.91SyphonophoresAmphipodsSergestoid Shrimps (protozoea) 60 10 5IsopodsPycnogonidsCumaceansOstracodsForaminiferans 60 0 1 0.50 30 1.23CtenophoresMysidsBryozoans (colonies)Cladocera 60 55 36 45.50 2730 111.75Unidentified



Veliger Larvae 60 150 162 156.00 9360 383.14Bivalve larvae 60 68 81Polychaete Larvae 60 2 4 3.00 180 7.37Cirriped Larvae 60 225 188 206.50 12390 507.16Ascidean Larvae 60 3 2 2.50 150 6.14Stomatopod Larvae 60ActinotrochsEchinoderm (Ophiuroidea ) 60 15 17 16.00 960 39.30Tanaidaceans

Unidentified

Fish Eggs Round 60 2 4 3.00 180 7.37 Pointed 0.00






Station: 5 Night Mesh:



Field Sample Code: #5 A N


Ind/Sample Ind/m 3

Calanoid Copepods 180 187 195 191.00 34380 1477.89Cyclopoid Copepods 180 5 3 4.00 720 30.95Harpacticoid Copepods 180 18 15 16.50 2970 127.69Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 180 10 13 11.50 2070 88.99Larvaceans 180 25 30 27.50 4950 212.81Cnidarians 180 2 3 2.50 450 19.35SyphonophoresAmphipodsSergestoid Shrimps (mainly adults) 180 11 15 13.00 2340 100.60Isopods 180 1 0 0.50 90 3.87PycnogonidsCumaceansOstracods 180 8 10 9.00 1620 69.65Foraminiferans 180 15 11 13.00 2340 100.60CtenophoresMysidsBryozoans (colonies)Cladocera 180 55 60 57.50 10350 444.97Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid 180 1 0 0.50 90 3.87 Caridean 180 14 12 13.00 2340 100.60 Anomuran 180 4 6 5.00 900 38.69 Macruran Brachyuran 180 2 1 1.50 270 11.61Decapod Eggs 180 3 5 4.00 720 30.95

Veliger Larvae 180 30 37 33.50 6030 259.24Bivalve larvaePolychaete Larvae 180 2 4 3.00 540 23.22Cirriped Larvae 180 94 110 102.00 18360 789.34Ascidean LarvaeStomatopod LarvaeActinotrochs 180 1 0 0.50 90 3.87Echinoderm (Ophiuroidea ) 180 8 12 10.00 1800 77.39Tanaidaceans

Unidentified 180 1 0 0.50 90 3.87



Total Meroplankton

TOTAL ZOOPLANKTON






Field Sample Code: 5B-N


Calanoid Copepods 100 191 184 187.50 18750 894.99Cyclopoid Copepods 100 7 5 6.00 600 28.64Harpacticoid Copepods 100 15 16 15.50 1550 73.99Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 100 11 8 9.50 950 45.35Larvaceans 100 27 25 26.00 2600 124.11Cnidarians 100 2 2 2.00 200 9.55Syphonophores 100Amphipods 100Sergestoid Shrimps 100 22 28 25.00 2500 119.33Isopods 100 0 1 0.50 50 2.39Pycnogonids 100Cumaceans 100Ostracods 100 12 13 12.50 1250 59.67Foraminiferans 100 8 6 7.00 700 33.41Ctenophores 100Mysids 100Bryozoans (colonies) 100Cladocera 100 48 41 44.50 4450 212.41Unidentified



Veliger Larvae 100 33 28 30.50 3050 145.58Bivalve larvae 100 4 3 3.50 350 16.71Polychaete Larvae 100 1 3 2.00 200 9.55Cirriped Larvae 100 107 121 114.00 11400 544.15Ascidean LarvaeStomatopod LarvaeActinotrochs 100 0 1 0.50 50 2.39Echinoderm (Ophiuroidea ) 100 5 10 7.50 750 35.80Tanaidaceans

Unidentified









Replicate: A Filtered Volume (m3): 47



Ind/Sample Ind/m 3

Calanoid Copepods 200 532 506 519.00 103800 2208.51Cyclopoid Copepods 200 49 47 48.00 9600 204.26Harpacticoid Copepods 200 8 8 8.00 1600 34.04Monstrilloid CopepodCaligoid CopepodCopepod Nauplii 200 2 0 1.00 200 4.26Chaetognath Worms 200 20 36 28.00 5600 119.15Larvaceans 200 27 23 25.00 5000 106.38Cnidarians 200 0 6 3.00 600 12.77SyphonophoresAmphipodsSergestoid Shrimps (mainly adults) 200 60 55 57.50 11500 244.68Isopods 200 3 2 2.50 500 10.64PycnogonidsCumaceansOstracodsForaminiferans 200 1 2 1.50 300 6.38Ctenophores 200Mysids 0 1 0.50 0 0.00Bryozoans (colonies) 200Cladocera 200 4 2 3.00 600 12.77Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid 200 0 2 1.00 200 4.26 Caridean 200 27 22 24.50 4900 104.26 Anomuran 200 0 2 1.00 200 4.26 Macruran Brachyuran 200 8 7 7.50 1500 31.91Decapod Eggs 200 9 5 7.00 1400 29.79Unidentified shrimp 200 2 0 1.00 200 4.26Veliger Larvae 200 21 20 20.50 4100 87.23Bivalve larvaePolychaete Larvae 200 7 13 10.00 2000 42.55Cirriped Larvae 200 36 32 34.00 6800 144.68Ascidean LarvaeStomatopod Larvae 200 0 2 1.00 200 4.26ActinotrochsEchinoderm (Ophiuroidea ) 200 11 4 7.50 1500 31.91Tanaidaceans

Unidentified







Station: 1 Night Mesh: 202



Field Sample Code: 1B-Night


Calanoid Copepods 500 116 127 121.50 60750 1439.23Cyclopoid Copepods 500 5 4 4.50 2250 53.30Harpacticoid Copepods 500 2 3 2.50 1250 29.61Monstrilloid Copepod 500 1 0 0.50 250 5.92Caligoid CopepodCopepod NaupliiChaetognath Worms 500 15 12 13.50 6750 159.91Larvaceans 500 11 14 12.50 6250 148.07Cnidarians 500 1 0 0.50 250 5.92SyphonophoresAmphipodsSergestoid Shrimps (mainly adults)500 40 47 43.50 21750 515.28Isopods 500 1 0 0.50 250 5.92PycnogonidsCumaceansOstracodsForaminiferansCtenophoresMysidsBryozoans (colonies)Cladocera 500 2 4 3.00 1500 35.54Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid Caridean 500 12 17 14.50 7250 171.76 Anomuran Macruran BrachyuranDecapod Eggs


Unidentified












Ind/Sample Ind/m 3

Calanoid Copepods 100 265 229 247.00 24700 942.92Cyclopoid Copepods 100 8 3 5.50 550 20.99Harpacticoid Copepods 100 24 13 18.50 1850 70.61Monstrilloid CopepodCaligoid Copepod 100 1 0 0.50 50 1.91Copepod Nauplii 100 2 0 1.00 100 3.82Chaetognath Worms 100 16 22 19.00 1900 72.52Larvaceans 100 28 30 29.00 2900 110.69Cnidarians 100 2 2 2.00 200 7.63Syphonophores 100 1 0 0.50 50 1.91AmphipodsSergestoid Shrimps (mainly adults) 100 8 3 5.50 550 20.99IsopodsPycnogonidsCumaceansOstracods 100 1 0 0.50 50 1.91ForaminiferansCtenophoresMysidsBryozoans (colonies) 100 2 2 2.00 200 7.63Cladocera 100 21 20 20.50 2050 78.24Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid 100 1 0 0.50 50 1.91 Caridean 100 2 0 1.00 100 3.82 Anomuran 100 1 0 Macruran Brachyuran 100 5 1 3.00 300 11.45Decapod Eggs 100 17 20 18.50 1850 70.61

Veliger Larvae 100 26 19 22.50 2250 85.88Bivalve larvae 100Polychaete Larvae 100 5 3 4.00 400 15.27Cirriped Larvae 100 32 24 28.00 2800 106.87Ascidean LarvaeStomatopod LarvaeActinotrochs 100 1 1 1.00 100 3.82Echinoderm (Ophiuroidea ) 100 8 8 8.00 800 30.53Tanaidaceans 100Planula 100 1 0 0.50 50 1.91Unidentified










Field Sample Code: 2B-Night


Calanoid Copepods 67 123 118 120.50 8033 345.07Cyclopoid Copepods 100 8 11 9.50 633 27.21Harpacticoid Copepods 100 5 7 6.00 600 25.77Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 100 15 18 16.50 1650 70.88Larvaceans 100 28 21 24.50 2450 105.24Cnidarians 100 1 0 0.50 50 2.15Syphonophores 100 0 1 0.50 50 2.15AmphipodsSergestoid Shrimps (mainly adults) 100 8 9 8.50 850 36.51IsopodsPycnogonidsCumaceansOstracods 100 0 1 0.50 50 2.15Foraminiferans 100 11 13 12.00 1200 51.55CtenophoresMysidsBryozoans (colonies)Cladocera 100 8 10 9.00 900 38.66Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid 100 0 1 0.50 50 2.15 Caridean 100 3 4 3.50 350 15.03 Anomuran Macruran Brachyuran 100 0 1 0.50 50 2.15Decapod Eggs 100 12 13 12.50 1250 53.69

0.00Veliger Larvae 100 13 14 13.50 1350 57.99Bivalve larvae 100 3 2 2.50 250 10.74Polychaete Larvae 100 8 5 6.50 650 27.92Cirriped Larvae 100 28 21 24.50 2450 105.24Ascidean LarvaeStomatopod Larvae 100 0 1 0.50 50 2.15ActinotrochsEchinoderm (Ophiuroidea ) 100 3 4 3.50 350 15.03Tanaidaceans

Unidentified








Date: Time: 8:51pm




Calanoid Copepods 100 183 189 186.00 18600 585.64Cyclopoid Copepods 100 11 9 10.00 1000 31.49Harpacticoid Copepods 100 42 37 39.50 3950 124.37Monstrilloid Copepod 100Caligoid Copepod 100Copepod Nauplii 100Chaetognath Worms 100 14 13 13.50 1350 42.51Larvaceans 100 50 43 46.50 4650 146.41Cnidarians 100 3 4 3.50 350 11.02Syphonophores 100Amphipods 100Sergestoid Shrimps (mainly adults) 100 25 20 22.50 2250 70.84Isopods 100Pycnogonids 100Cumaceans 100Ostracods 100 3 4 3.50 350 11.02Foraminiferans 100 5 3 4.00 400 12.59Ctenophores 100Mysids 100Bryozoans (colonies) 100Cladocera 100 36 43 39.50 3950 124.37Unidentified 100



Veliger Larvae 100 8 17 12.50 1250 39.36Bivalve larvae 100Polychaete Larvae 100 4 3 3.50 350 11.02Cirriped Larvae 100 242 246 244.00 24400 768.26Ascidean LarvaeStomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 100 18 16 17.00 1700 53.53Tanaidaceans

Unidentified

Fish Eggs Round 100 3 5 4.00 400 12.59 Pointed 100









Field Sample Code: 3B-N


Calanoid Copepods 100 88 107 97.50 9750 281.61Cyclopoid Copepods 100 5 4 4.50 450 13.00Harpacticoid Copepods 100 6 8 7.00 700 20.22Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 100 14 13 13.50 1350 38.99Larvaceans 100 36 66 51.00 5100 147.31Cnidarians 100 1 4 2.50 250 7.22SyphonophoresAmphipods 100 1 0 0.50 50 1.44Sergestoid Shrimps (protozea) 100 17 42 29.50 2950 85.21IsopodsPycnogonidsCumaceansOstracods 100 7 8 7.50 750 21.66Foraminiferans 100 5 1 3.00 300 8.67CtenophoresMysids 100 2 0 1.00 100 2.89Bryozoans (cyphonautes)Cladocera 100 15 29 22.00 2200 63.55Unidentified




Unidentified







Station: Discharge (7) Mesh:

Date: October 12, 2012 Time: 9:06am


Field Sample Code: Discharge A Day


Ind/Sample Ind/m 3

Calanoid Copepods 100 250 227 238.50 23850 980.34Cyclopoid Copepods 100 12 7 9.50 950 39.05Harpacticoid Copepods 100 24 19 21.50 2150 88.37Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 100 19 15 17.00 1700 69.87Larvaceans 100 23 26 24.50 2450 100.70Cnidarians 100 2 0 1.00 100 4.11SyphonophoresAmphipodsSergestoid Shrimps (mainly adults) 100 3 5 4.00 400 16.44IsopodsPycnogonidsCumaceansOstracodsForaminiferans 100 0 1 0.50 50 2.06CtenophoresMysidsBryozoans (colonies) 100 1 0Cladocera 100 23 26 24.50 2450 100.70Unidentified



Veliger Larvae 100 112 98 105.00 10500 431.57Bivalve larvae 100Polychaete Larvae 100 9 7 8.00 800 32.88Cirriped Larvae 100 129 117 123.00 12300 505.55Ascidean LarvaeStomatopod LarvaeActinotrochs 100 1 0 0.50 50 2.06Echinoderm (Ophiuroidea ) 100 5 4 4.50 450 18.50TanaidaceansPlanula 100 4 1 2.50 250 10.28Unidentified







Station: Discharge (7) Mesh:



Field Sample Code: Discharge B -Day


Calanoid Copepods 100 198 189 193.50 19350 907.28Cyclopoid Copepods 100 10 12 11.00 1100 51.57Harpacticoid Copepods 100 22 18 20.00 2000 93.76Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 100 17 22 19.50 1950 91.42Larvaceans 100 18 23 20.50 2050 96.11Cnidarians 100 1 0 0.50 50 2.34SyphonophoresAmphipodsSergestoid Shrimps 100 10 12 11.00 1100 51.57IsopodsPycnogonidsCumaceansOstracodsForaminiferans 100 1 0 0.50 50 2.34CtenophoresMysidsBryozoans (colonies)Cladocera 100 32 25 28.50 2850 133.61Unidentified



Veliger Larvae 100 50 37 43.50 4350 203.94Bivalve larvae 100 40 28 34.00 3400 159.40Polychaete Larvae 100 7 6 6.50 650 30.47Cirriped Larvae 100 120 103 111.50 11150 522.74Ascidean LarvaeStomatopod LarvaeActinotrochs 100 1 0 0.50 50 2.34Echinoderm (Ophiuroidea ) 100 5 4 4.50 450 21.10TanaidaceansPlanula 100 4 1 2.50 250 11.72Unidentified







Station: Discharge (7) Night Mesh:

Date: Time: 9:42pm


Field Sample Code: Discharge A N


Ind/Sample Ind/m 3

Calanoid Copepods 100 113 103 108.00 10800 494.89Cyclopoid Copepods 100 31 32 31.50 3150 144.36Harpacticoid Copepods 100 13 22 17.50 1750 80.20Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 100 4 4 4.00 400 18.33Larvaceans 100 63 41 52.00 5200 238.31Cnidarians 100 4 4 4.00 400 18.33SyphonophoresAmphipodsSergestoid Shrimps (mainly adults) 100 13 9 11.00 1100 50.41Isopods 100 1 0 0.50 50 2.29PycnogonidsCumaceansOstracods 100 1 0 0.50 50 2.29ForaminiferansCtenophoresMysidsBryozoans (colonies)Cladocera 100 61 42 51.50 5150 236.02Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid 100 7 1 4.00 400 18.33 Caridean 100 1 0 0.50 50 2.29 Anomuran Macruran Brachyuran 100 2 1 1.50 150 6.87Decapod Eggs 100 10 9 9.50 950 43.54

Veliger Larvae 100 31 16 23.50 2350 107.70Bivalve larvaePolychaete Larvae 100 1 1 1.00 100 4.58Cirriped Larvae 100 128 106 117.00 11700 536.21Ascidean LarvaeStomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 100 11 9 10.00 1000 45.83Tanaidaceans

Unidentified







Station: Discharge (7) Night Mesh: 202



Field Sample Code: Discharge B-N


Calanoid Copepods 100 103 113 108.00 10800 556.33Cyclopoid Copepods 100 5 10 7.50 750 38.64Harpacticoid Copepods 100 7 10 8.50 850 43.79Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 100 14 5 9.50 950 48.94Larvaceans 100 45 58 51.50 5150 265.33Cnidarians 100 3 4 3.50 350 18.03SyphonophoresAmphipodsSergestoid Shrimps 100 36 25 30.50 3050 157.14Isopods 100 1 0 0.50 50 2.58PycnogonidsCumaceansOstracods 100 7 5 6.00 600 30.91Foraminiferans 100 1 0 0.50 50 2.58CtenophoresMysidsBryozoans 100 1 0 0.50 50 2.58Cladocera 100 40 49 44.50 4450 229.26Unidentified




Unidentified







Station: Intake (6) Night Mesh:

Date: Time: 9:17pm


Field Sample Code: Intake A N


Ind/Sample Ind/m 3

Calanoid Copepods 100 163 173 168.00 16800 563.71Cyclopoid Copepods 100 9 14 11.50 1150 38.59Harpacticoid Copepods 100 2 4 3.00 300 10.07Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 100 11 10 10.50 1050 35.23Larvaceans 100 38 26 32.00 3200 107.38Cnidarians 100 2 0 1.00 100 3.36SyphonophoresAmphipodsSergestoid Shrimps (mainly adults) 100 25 21 23.00 2300 77.18IsopodsPycnogonidsCumaceansOstracods 100 3 0 1.50 150 5.03ForaminiferansCtenophoresMysidsBryozoans (colonies)Cladocera 100 14 21 17.50 1750 58.72Unidentified



Veliger Larvae 100 3 6 4.50 450 15.10Bivalve larvaePolychaete Larvae 100 2 3 2.50 250 8.39Cirriped Larvae 100 109 101 105.00 10500 352.35Ascidean LarvaeStomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 100 12 13 12.50 1250 41.95Tanaidaceans

Unidentified







Station: Intake (6) Night Mesh: 202



Field Sample Code: Intake B-N


Calanoid Copepods 200 88 112 100.00 20000 817.31Cyclopoid Copepods 200 2 1 1.50 300 12.26Harpacticoid Copepods 200 3 2 2.50 500 20.43Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 200 3 2 2.50 500 20.43Larvaceans 200 26 39 32.50 6500 265.63Cnidarians 200 1 0 0.50 100 4.09SyphonophoresAmphipodsSergestoid Shrimps 200 27 36 31.50 6300 257.46IsopodsPycnogonidsCumaceansOstracods 200 4 5 4.50 900 36.78ForaminiferansCtenophoresMysidsBryozoans 200 1 0 0.50 100 4.09Cladocera 200 21 21 21.00 4200 171.64Unidentified




Unidentified


Fish Larvae (Unknown) *





Station: Intake (6) Mesh:



Field Sample Code: Intake A Day


Ind/Sample Ind/m 3

Calanoid Copepods 120 161 146 153.50 18420 633.86Cyclopoid Copepods 120 15 6 10.50 1260 43.36Harpacticoid Copepods 120 12 24 18.00 2160 74.33Monstrilloid CopepodCaligoid CopepodCopepod Nauplii 120 0 1 0.50 60 2.06Chaetognath Worms 120 3 9 6.00 720 24.78Larvaceans 120 16 11 13.50 1620 55.75Cnidarians 120 1 2 1.50 180 6.19Syphonophores 120 1 0 0.50 60 2.06Amphipods 120Sergestoid Shrimps (mainly adults) 120 3 1 2.00 240 8.26Isopods 120 0 1 0.50 60 2.06Pycnogonids 0.00CumaceansOstracods 120 1 0 0.50 60 2.06Foraminiferans 120 4 2 3.00 360 12.39Ctenophores 0.00MysidsBryozoans (colonies) 120 4 4 4.00 480 16.52Cladocera 120 20 26 23.00 2760 94.98Unidentified



Veliger Larvae 120 76 62 69.00 8280 284.93Bivalve larvaePolychaete Larvae 120 4 5 4.50 540 18.58Cirriped Larvae 120 146 148 147.00 17640 607.02Ascidean LarvaeStomatopod LarvaeActinotrochsEchinoderm (Ophiuroidea ) 120 5 7 6.00 720 24.78TanaidaceansPlanula 120 1 0 0.50 60 2.06UnidentifiedEchinoderm (Bipinnaria) 120 0 1 0.50 60 2.06Fish Eggs Round 120 2 2 2.00 240 8.26 Pointed






Station: Intake (6) Mesh: 202



Field Sample Code: Intake B-D


Calanoid Copepods 100 127 164 145.50 14550 494.90Cyclopoid Copepods 100 30 16 23.00 2300 78.23Harpacticoid Copepods 100 8 16 12.00 1200 40.82Monstrilloid CopepodCaligoid CopepodCopepod NaupliiChaetognath Worms 100 10 5 7.50 750 25.51Larvaceans 100 10 20 15.00 1500 51.02Cnidarians 100 1 0 0.50 50 1.70SyphonophoresAmphipodsSergestoid Shrimps (adults) 100 18 28 23.00 2300 78.23IsopodsPycnogonidsCumaceansOstracodsForaminiferans 100 1 0 0.50 50 1.70CtenophoresMysidsBryozoans 100 2 0 1.00 100 3.40Cladocera 100 29 33 31.00 3100 105.44Unidentified


MEROPLANKTON TAXA MeanDecapod Larvae Penaeoid 100 2 0 1.00 100 3.40 Caridean Anomuran Macruran Brachyuran 100 1 0 0.50 50 1.70Decapod Eggs

Veliger Larvae 100 20 17 18.50 1850 62.93Bivalve larvae 100 39 34 36.50 3650 124.15Polychaete Larvae 100 9 1 5.00 500 17.01Cirriped Larvae 100 121 105 113.00 11300 384.35Ascidean LarvaeStomatopod LarvaeActinotrochs 100 1 0 0.50 50 1.70Echinoderm (Ophiuroidea ) 100 4 4 4.00 400 13.61Tanaidaceans

Unidentified







Verification of Ichthyoplankton Counts

Station>>>>> 1 2 3 4 5 6 7 8 9 10 11 12 13

Ichthyoplankton Taxa Counts Per Sample

Total fish larvae 2 155 5 7 7 5 5 7 44 4 20 44 3

Total Fish Eggs 111 164 100 182 129 236 109 1124 88 120 141 142 72

Total Ichthyoplankton 113 319 105 189 136 241 114 1131 132 124 161 186 75

Ichthyoplankton Taxa Abundance (Ind/m3)

Total fish larvae 0.08 4.58 0.20 0.33 0.30 0.17 0.23 0.17 1.46 0.14 0.45 1.32 0.12

Total Fish Eggs 4.30 4.90 3.90 8.70 5.50 8.00 5.09 26.64 2.91 4.05 3.14 4.26 2.95

Total Ichthyoplankton 4.38 9.48 4.10 9.03 5.80 8.17 5.32 26.80 4.37 4.19 3.59 5.58 3.07



Appendix 3. Statistical Analyses

SUMMARY OF MULTIPLE COMPARISON ANALYSIS OF TOTAL ZOOPLANKTON ABUNDANCE

AMONG DIFFERENT STATIONS

Multiple Comparisons Student-Newman-Keuls For Total Zooplankton , classified by Stations Mean Square Error: 553894.665151022, Degrees of Freedom: 13

Homogeneous

Subsets:

Group 1: 12 10 4 5 2 13 6 9 11 3 7

Pooled mean = 1568.5022

95% Confidence

Interval = 1225.7106 1911.2937 Group 2: 2 13 6 9 11 3 7 1 Pooled mean = 2239.5365

95% Confidence

Interval = 1837.5777 2641.4952 Group 3: 8.0000 Pooled mean = 8688.0862

95% Confidence

Interval = 7551.1753 9824.9972

Between Homogeneous

subsets: Group 2 - Group 1 Difference = 671.0343

95% Confidence

Interval = -301.6423 1643.7109 Group 3 - Group 2 Difference = 6448.5498

95% Confidence

Interval = 4228.2620 8668.8375



SUMMARY OF MULTIPLE COMPARISONS ANALYSIS OF HOLOPLANKTON ABUNDANCE MINUS

CALANOIDS ABUNDANCE AMONG DIFFERENT STATIONS.

Multiple Comparisons Student-Newman-Keuls For HOLO-CALA, classified by C1 Mean Square Error: 53192.9584739199, Degrees of Freedom: 13

Homogeneous

Subsets:

Group 1: 10 12 11 13 4 5 7 8 3 2 6 1


95% Confidence


95% Confidence

Interval = 1034.5969 1739.2412

Between Homogeneous


95% Confidence

Interval = 384.1301 1734.5149



SUMMARY OF MULTIPLE COMPARISONS ANALYSIS OF MEROPLANKTON ABUNDANCE AMONG

DIFFERENT STATIONS.

Student-Newman-Keuls For MERO, classified by C1 Mean Square Error: 31824.9762552718, Degrees of Freedom: 13

Homogeneous

Subsets:

Group 1: 12 10 4 2 9 8 5


95% Confidence

Interval = 185.6041 391.6092

Group 2: 4 2 9 8 5 3 7 13


95% Confidence

Interval = 441.3762 634.0763

Group 3: 3 7 13 6 11


95% Confidence


95% Confidence

Interval = 2102.2368 2647.2749

Between Homogeneous


95% Confidence

Interval = -10.5703 508.8096 Group 3 - Group 2 Difference = 380.0459

95% Confidence

Interval = 93.9938 666.0980 Group 4 - Group 3 Difference = 1456.9837 95% Confidence 907.3237 2006.6436



Interval =

BRAY CURTIS HIERARCHICAL CLUSTER ANALYSIS USING HOLOPLANKTON(NO CALANOIDS)

AND MEROPPLANKTON RESULTS. Hierarchical Clustering Results for:

Quantitative Data Set = Replicate no calanoids (2!$Y$69:$AA$81

Distance/Similarity Measure = Bray and Curtis

Cluster Method = Nearest Neighbour

Distance Matrix (Bray and Curtis)

1 2 3 4 5 8 9 10 11 12 13 DIS IN

1 0.579 0.441 0.665 0.586 0.591 0.615 0.832 0.394 0.841 0.420 0.318 0.426

2 0.579 0.245 0.139 0.128 0.066 0.385 0.489 0.475 0.511 0.422 0.319 0.285

3 0.441 0.245 0.316 0.195 0.203 0.479 0.618 0.210 0.636 0.156 0.142 0.038

4 0.665 0.139 0.316 0.129 0.121 0.497 0.375 0.428 0.400 0.367 0.439 0.332

5 0.586 0.128 0.195 0.129 0.064 0.442 0.480 0.381 0.503 0.324 0.329 0.213

8 0.591 0.066 0.203 0.121 0.064 0.406 0.474 0.431 0.496 0.376 0.336 0.236

9 0.615 0.385 0.479 0.497 0.442 0.406 0.735 0.624 0.748 0.593 0.488 0.502

10 0.832 0.489 0.618 0.375 0.480 0.474 0.735 0.652 0.094 0.633 0.699 0.629

11 0.394 0.475 0.210 0.428 0.381 0.431 0.624 0.652 0.668 0.057 0.106 0.170

12 0.841 0.511 0.636 0.400 0.503 0.496 0.748 0.094 0.668 0.650 0.713 0.646

13 0.420 0.422 0.156 0.367 0.324 0.376 0.593 0.633 0.057 0.650 0.118 0.116

DIS 0.318 0.319 0.142 0.439 0.329 0.336 0.488 0.699 0.106 0.713 0.118 0.125

IN 0.426 0.285 0.038 0.332 0.213 0.236 0.502 0.629 0.170 0.646 0.116 0.125

Clustering Strategy

Cluster 1st Item 2nd Item Distance

1 IN 3.000 0.038

2 13.000 11.000 0.057

3 8.000 5.000 0.064

4 Cluster 3 2.000 0.066

5 12.000 10.000 0.094

6 Cluster 2 DIS 0.106

7 Cluster 6 Cluster 1 0.116

8 Cluster 4 4.000 0.121


10 Cluster 9 1.000 0.318


12 Cluster 11 9.000 0.385

Cophenetic Correlation

R DF P

0.870 76 0.000



Dendogram of above results. Numbers on the right are station designation. Numbers at each node represent distance among groups.

TWO WAY ANALYSIS OF VARIANCE EVALUATING STATISTICAL DIFFERENCES BETWEEN

PLANKTONIC PLANKTONIC GOUPS DURING DAY AND NIGHT SAMPLINGS.



Analysis of Variance results for: Holoplankton

Y Variable Range = $D$4:$D$27

Factor Range = $A$4:$B$27

Descriptive Statistics

Group Mean Std Dev. Std Err N

1, d 1614.121 653.030 461.762 2

1, n 2682.340 401.099 283.620 2

2, d 1063.407 204.870 144.865 2

2, n 1024.555 448.622 317.224 2

3, d 1614.707 276.097 195.230 2

3, n 926.020 331.272 234.245 2

5, d 696.998 383.375 271.087 2

5, n 2140.597 759.119 536.779 2

6, d 929.809 69.093 48.856 2

6, n 1254.699 502.634 355.416 2

7, d 1415.829 20.073 14.194 2

7, n 1340.775 78.245 55.328 2

Overall test of model for Y=Column D

Source Type III SS Df Mean Sq. F Prob.

Model 7006262.212 11 ######## 3.815 0.015

Error 2003712.529 12 ########

Total 9009974.741 23

Tests of effects for Y=Column D


Column A 3194203.917 5 ######## 3.826 0.026

Column B 689604.523 1 ######## 4.130 0.065

Column A*Column B 3122453.772 5 ######## 3.740 0.028

Post hoc tests were not performed for factor = Column B

as it has less than 3 groups.

Post Hoc tests for Factor = Column A

Test Group 1 Group 2 Mean Diff. SE q Prob.

Student-Newman-Keuls1 2 1104.250 204.314 5.405 0.023

3 877.867 204.314 4.297 0.044

5 729.433 204.314 3.570 0.027

6 1055.977 204.314 5.168 0.022

7 769.929 204.314 3.768 0.050

2 3 -226.382 204.314 1.108 0.720

5 -374.817 204.314 1.835 0.698

6 -48.273 204.314 0.236 0.870

7 -334.321 204.314 1.636 0.663

3 5 -148.434 204.314 0.727 0.866

6 178.109 204.314 0.872 0.549

7 -107.939 204.314 0.528 0.715

5 6 326.543 204.314 1.598 0.679

7 40.496 204.314 0.198 0.891

6 7 -286.048 204.314 1.400 0.597



Analysis of Variance results for: Meroplankton

Y Variable Range = $E$4:$E$27

Factor Range = $A$4:$B$27

Descriptive Statistics

Group Mean Std Dev. Std Err N

1, d 2374.756 465.454 329.126 2

1, n 697.634 261.429 184.858 2

2, d 331.038 2.324 1.643 2

2, n 376.499 34.316 24.265 2

3, d 740.880 312.916 221.265 2

3, n 735.646 386.766 273.485 2

5, d 421.322 150.383 106.337 2

5, n 1201.267 429.768 303.892 2

6, d 805.173 251.195 177.622 2

6, n 582.114 130.364 92.181 2

7, d 1032.140 34.181 24.170 2

7, n 774.243 45.760 32.357 2

Overall test of model for Y=Column E


Model ######## 11 ######## 8.695 0.000

Error ######## 12 ########

Total ######## 23

Tests of effects for Y=Column E


Column A ######## 5 ######## 8.824 0.001

Column B ######## 1 ######## 4.343 0.059

Column A*Column B######## 5 ######## 9.436 0.001

Post hoc tests were not performed for factor = Column B

as it has less than 3 groups.

Post Hoc tests for Factor = Column A

Test Group 1 Group 2 Mean Diff. SE q Prob.

Student-Newman-Keuls1 2 1182.427 131.050 9.023 0.000

3 797.932 131.050 6.089 0.005

5 724.900 131.050 5.531 0.005

6 842.552 131.050 6.429 0.005

7 633.004 131.050 4.830 0.005

2 3 -384.495 131.050 2.934 0.137

5 -457.527 131.050 3.491 0.116

6 -339.875 131.050 2.593 0.092

7 -549.423 131.050 4.192 0.073

3 5 -73.032 131.050 0.557 0.700

6 44.620 131.050 0.340 0.814

7 -164.928 131.050 1.259 0.657

5 6 117.651 131.050 0.898 0.804

7 -91.896 131.050 0.701 0.629

6 7 -209.548 131.050 1.599 0.679



Appendix 4. Results of Different Entrainment Scenarios using

plankton concentrations from differnet stations.

Assumptions Volume and Flow Assumption Inputs. See text for references.

Bay volume(m3) 4.43E+07

tidal volume(m3) 4.30E+05

total volume (m3) 4.48E+07

Intake vdaily volume (m3/d) 54473.8

Descriptors

1. Intake station: number of the station which plankton abundance was used for the concentration at the intake;

2. Intake abundance: plankton abundance found at the stations considered the intake station for calculation purposes;

3. Background Station: Station considered representative of Guayanilla Bay for the purpose of the calculations;

4. Background Abundance: plankton abundance at the station considered representative of Guayanilla Bay for the purpose of the calculations;

5. Esig= the significance of the number of entrained plankton is the proportion of the total number of plankton in Guayanilla bay to the total number entrained. See text for formula. This number is calculated using the volume and flow assumptions mentioned above;

6. % Entrained= 1/Esig x 100; 7. Fish Adult Estimate from larvae= the abundance of larvae divided by 10; 8. Fish Adult Estimate from Eggs= the abundance of eggs divided by 10 per each

trophic step (2).



Zooplankton Estimates

Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

8 8657 12 472 45 2.23

8 8657 10 654 62 1.61

1 3977 12 472 97 1.03

1 3977 10 654 135 0.74

8 8657 13 1558 148 0.68

7 2434 12 472 159 0.63

3 2358 12 472 164 0.61

11 2255 12 472 172 0.58

9 2151 12 472 180 0.55

8 8657 9 2151 204 0.49

8 8657 11 2255 214 0.47

1 3977 4 1057 218 0.46

7 2434 10 654 221 0.45

6 1725 12 472 225 0.45

1 3977 5 1093 226 0.44

3 2358 10 654 228 0.44

9 2151 10 654 250 0.40

2 1391 12 472 279 0.36

1 3977 2 1391 287 0.35

6 1725 10 654 312 0.32

1 3977 13 1558 322 0.31

5 1093 12 472 354 0.28

1 3977 6 1725 356 0.28

4 1057 12 472 367 0.27

3 2358 4 1057 368 0.27

3 2358 5 1093 381 0.26

2 1391 10 654 386 0.26

1 3977 9 2151 444 0.23

1 3977 11 2255 466 0.21

1 3977 3 2358 487 0.21

5 1093 10 654 492 0.20

1 3977 7 2434 503 0.20

4 1057 10 654 509 0.20

7 2434 13 1558 526 0.19

3 2358 13 1558 543 0.18

11 2255 13 1558 568 0.18

10 654 12 472 592 0.17

9 2151 13 1558 595 0.17




Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

3 2358 6 1725 601 0.17

2 1391 4 1057 624 0.16

2 1391 5 1093 646 0.15

7 2434 9 2151 726 0.14

6 1725 13 1558 742 0.13

3 2358 9 2151 749 0.13

7 2434 11 2255 761 0.13

3 2358 11 2255 786 0.13

10 654 10 654 822 0.12

4 1057 4 1057 822 0.12

5 1093 5 1093 822 0.12

2 1391 2 1391 822 0.12

11 2255 11 2255 822 0.12

3 2358 3 2358 822 0.12

7 2434 7 2434 822 0.12

1 3977 1 3977 822 0.12

8 8657 8 8657 822 0.12

12 472 12 472 822 0.12

13 1558 13 1558 822 0.12

6 1725 6 1725 822 0.12

9 2151 9 2151 822 0.12

3 2358 7 2434 848 0.12

4 1057 5 1093 850 0.12

9 2151 11 2255 862 0.12

2 1391 13 1558 921 0.11

2 1391 6 1725 1019 0.10

6 1725 9 2151 1025 0.10

6 1725 11 2255 1074 0.09

6 1725 7 2434 1159 0.09

5 1093 13 1558 1171 0.09

4 1057 13 1558 1212 0.08

2 1391 9 2151 1271 0.08

5 1093 6 1725 1296 0.08

2 1391 11 2255 1333 0.08

4 1057 6 1725 1341 0.07

2 1391 3 2358 1393 0.07

2 1391 7 2434 1438 0.07

5 1093 9 2151 1617 0.06

4 1057 9 2151 1672 0.06

5 1093 11 2255 1695 0.06




Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

4 1057 11 2255 1754 0.06

1 3977 8 8657 1789 0.06

5 1093 7 2434 1829 0.05

4 1057 7 2434 1892 0.05

10 654 13 1558 1957 0.05

12 472 13 1558 2715 0.04

10 654 11 2255 2833 0.04

7 2434 8 8657 2923 0.03

3 2358 8 8657 3017 0.03

6 1725 8 8657 4124 0.02

2 1391 8 8657 5115 0.02

5 1093 8 8657 6507 0.02

4 1057 8 8657 6731 0.01



Fish larvae Fish Adult Estimate from Larvae

Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

Intake

Estimate

Background

Estimate

2 4.58 13 0.12 22 4.644 0.46 0.01

2 4.58 10 0.14 25 3.981 0.46 0.01

5 3.82 13 0.12 26 3.873 0.38 0.01

5 3.82 10 0.14 30 3.320 0.38 0.01

2 4.58 6 0.17 31 3.278 0.46 0.02

2 4.58 8 0.17 31 3.278 0.46 0.02

2 4.58 3 0.20 35 2.829 0.46 0.02

5 3.82 6 0.17 37 2.734 0.38 0.02

5 3.82 8 0.17 37 2.734 0.38 0.02

2 4.58 7 0.23 42 2.382 0.46 0.02

5 3.82 7 0.23 50 1.986 0.38 0.02

2 4.58 4 0.33 60 1.668 0.46 0.03

9 1.46 13 0.12 68 1.481 0.15 0.01

12 1.32 13 0.12 75 1.339 0.13 0.01

9 1.46 10 0.14 79 1.269 0.15 0.01

2 4.58 11 0.45 81 1.238 0.46 0.05

5 3.82 11 0.45 97 1.033 0.38 0.05

11 0.45 13 0.12 219 0.456 0.05 0.01

2 4.58 12 1.32 237 0.422 0.46 0.13

9 1.46 11 0.45 253 0.395 0.15 0.05

2 4.58 9 1.46 262 0.382 0.46 0.15

5 3.82 12 1.32 284 0.352 0.38 0.13

4 0.33 13 0.12 295 0.339 0.03 0.01

5 3.82 9 1.46 314 0.318 0.38 0.15

4 0.33 10 0.14 344 0.290 0.03 0.01

4 0.33 6 0.17 418 0.239 0.03 0.02

4 0.33 8 0.17 418 0.239 0.03 0.02

7 0.23 13 0.12 421 0.237 0.02 0.01

7 0.23 10 0.14 492 0.203 0.02 0.01

3 0.20 13 0.12 501 0.200 0.02 0.01

4 0.33 7 0.23 576 0.174 0.03 0.02

6 0.17 13 0.12 580 0.172 0.02 0.01

8 0.17 13 0.12 580 0.172 0.02 0.01

3 0.20 10 0.14 584 0.171 0.02 0.01

7 0.23 8 0.17 597 0.168 0.02 0.02

6 0.17 10 0.14 677 0.148 0.02 0.01

8 0.17 10 0.14 677 0.148 0.02 0.01

2 4.58 5 3.82 685 0.146 0.46 0.38




Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

Intake

Estimate

Background

Estimate

10 0.14 13 0.12 704 0.142 0.01 0.01

3 0.20 6 0.17 709 0.141 0.02 0.02

3 0.20 8 0.17 709 0.141 0.02 0.02

9 1.46 12 1.32 743 0.135 0.15 0.13

1 0.08 1 0.08 822 0.122 0.01 0.01

13 0.12 13 0.12 822 0.122 0.01 0.01

3 0.20 3 0.20 822 0.122 0.02 0.02

7 0.23 7 0.23 822 0.122 0.02 0.02

4 0.33 4 0.33 822 0.122 0.03 0.03

11 0.45 11 0.45 822 0.122 0.05 0.05

12 1.32 12 1.32 822 0.122 0.13 0.13

5 3.82 5 3.82 822 0.122 0.38 0.38

2 4.58 2 4.58 822 0.122 0.46 0.46

10 0.14 10 0.14 822 0.122 0.01 0.01

6 0.17 6 0.17 822 0.122 0.02 0.02

6 0.17 8 0.17 822 0.122 0.02 0.02

8 0.17 8 0.17 822 0.122 0.02 0.02

9 1.46 9 1.46 822 0.122 0.15 0.15

3 0.20 7 0.23 976 0.102 0.02 0.02

4 0.33 11 0.45 1107 0.090 0.03 0.05

6 0.17 7 0.23 1131 0.088 0.02 0.02

1 0.08 13 0.12 1281 0.078 0.01 0.01

3 0.20 4 0.33 1393 0.072 0.02 0.03

1 0.08 10 0.14 1494 0.067 0.01 0.01

7 0.23 11 0.45 1580 0.063 0.02 0.05

1 0.08 6 0.17 1814 0.055 0.01 0.02

1 0.08 8 0.17 1814 0.055 0.01 0.02

3 0.20 11 0.45 1877 0.053 0.02 0.05

1 0.08 3 0.20 2102 0.048 0.01 0.02

6 0.17 11 0.45 2175 0.046 0.02 0.05

8 0.17 11 0.45 2175 0.046 0.02 0.05

11 0.45 12 1.32 2410 0.041 0.05 0.13

1 0.08 7 0.23 2497 0.040 0.01 0.02

10 0.14 11 0.45 2641 0.038 0.01 0.05

4 0.33 12 1.32 3247 0.031 0.03 0.13

1 0.08 4 0.33 3564 0.028 0.01 0.03

4 0.33 9 1.46 3592 0.028 0.03 0.15

7 0.23 12 1.32 4635 0.022 0.02 0.13

1 0.08 11 0.45 4802 0.021 0.01 0.05

7 0.23 9 1.46 5127 0.020 0.02 0.15




Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

Intake

Estimate

Background

Estimate

3 0.20 12 1.32 5506 0.018 0.02 0.13

3 0.20 9 1.46 6090 0.016 0.02 0.15

6 0.17 12 1.32 6380 0.016 0.02 0.13

8 0.17 12 1.32 6380 0.016 0.02 0.13

6 0.17 9 1.46 7057 0.014 0.02 0.15

8 0.17 9 1.46 7057 0.014 0.02 0.15

10 0.14 12 1.32 7747 0.013 0.01 0.13

4 0.33 5 3.82 9395 0.011 0.03 0.38

1 0.08 12 1.32 14086 0.007 0.01 0.13

1 0.08 9 1.46 15580 0.006 0.01 0.15

3 0.20 5 3.82 15929 0.006 0.02 0.38

1 0.08 5 3.82 40753 0.002 0.01 0.38

1 0.08 2 4.58 48863 0.002 0.01 0.46



Fish Eggs Fish Adult Estimate from Eggs

Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

Intake

Estimate

Background

Estimate

8 26.64 9 2.91 90 1.11 0.27 0.03

8 26.64 13 2.95 91 1.10 0.27 0.03

8 26.64 11 3.14 97 1.03 0.27 0.03

8 26.64 10 4.05 125 0.80 0.27 0.04

8 26.64 12 4.26 131 0.76 0.27 0.04

4 8.687 9 2.91 275 0.36 0.09 0.03

4 8.687 13 2.95 279 0.36 0.09 0.03

4 8.687 11 3.14 297 0.34 0.09 0.03

6 8.028 9 2.91 298 0.34 0.08 0.03

6 8.028 13 2.95 302 0.33 0.08 0.03

6 8.028 11 3.14 321 0.31 0.08 0.03

4 8.687 10 4.05 383 0.26 0.09 0.04

4 8.687 12 4.26 403 0.25 0.09 0.04

6 8.028 10 4.05 415 0.24 0.08 0.04

6 8.028 12 4.26 436 0.23 0.08 0.04

5 5.31 9 2.91 450 0.22 0.05 0.03

5 5.31 13 2.95 456 0.22 0.05 0.03

7 5.11 9 2.91 468 0.21 0.05 0.03

7 5.11 13 2.95 474 0.21 0.05 0.03

4 8.687 7 5.11 483 0.21 0.09 0.05

5 5.31 11 3.14 486 0.21 0.05 0.03




Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

Intake

Estimate

Background

Estimate

2 4.845 9 2.91 494 0.20 0.05 0.03

2 4.845 13 2.95 500 0.20 0.05 0.03

4 8.687 5 5.31 502 0.20 0.09 0.05

7 5.11 11 3.14 505 0.20 0.05 0.03

6 8.028 7 5.11 523 0.19 0.08 0.05

2 4.845 11 3.14 533 0.19 0.05 0.03

1 4.292 9 2.91 557 0.18 0.04 0.03

1 4.292 13 2.95 565 0.18 0.04 0.03

12 4.26 13 2.95 569 0.18 0.04 0.03

10 4.05 13 2.95 599 0.17 0.04 0.03

1 4.292 11 3.14 601 0.17 0.04 0.03

3 3.943 9 2.91 606 0.16 0.04 0.03

3 3.943 13 2.95 615 0.16 0.04 0.03

5 5.31 10 4.05 627 0.16 0.05 0.04

10 4.05 11 3.14 637 0.16 0.04 0.03

7 5.11 10 4.05 651 0.15 0.05 0.04

3 3.943 11 3.14 654 0.15 0.04 0.03

5 5.31 12 4.26 659 0.15 0.05 0.04

2 4.845 3 3.943 669 0.15 0.05 0.04

7 5.11 12 4.26 685 0.15 0.05 0.04

2 4.845 10 4.05 687 0.15 0.05 0.04

2 4.845 12 4.26 722 0.14 0.05 0.04




Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

Intake

Estimate

Background

Estimate

1 4.292 3 3.943 755 0.13 0.04 0.04

4 8.687 6 8.028 759 0.13 0.09 0.08

11 3.14 13 2.95 772 0.13 0.03 0.03

1 4.292 10 4.05 775 0.13 0.04 0.04

5 5.31 7 5.11 791 0.13 0.05 0.05

1 4.292 12 4.26 816 0.12 0.04 0.04

3 3.943 3 3.943 822 0.12 0.04 0.04

9 2.91 9 2.91 822 0.12 0.03 0.03

13 2.95 13 2.95 822 0.12 0.03 0.03

10 4.05 10 4.05 822 0.12 0.04 0.04

2 4.845 2 4.845 822 0.12 0.05 0.05

7 5.11 7 5.11 822 0.12 0.05 0.05

5 5.31 5 5.31 822 0.12 0.05 0.05

6 8.028 6 8.028 822 0.12 0.08 0.08

8 26.64 8 26.64 822 0.12 0.27 0.27

11 3.14 11 3.14 822 0.12 0.03 0.03

12 4.26 12 4.26 822 0.12 0.04 0.04

1 4.29200 1 4.29200 822 0.12 0.04 0.04

4 8.687 4 8.687 822 0.12 0.09 0.09

9 2.91 13 2.95 833 0.12 0.03 0.03

3 3.943 10 4.05 844 0.12 0.04 0.04

10 4.05 12 4.26 864 0.12 0.04 0.04




Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

Intake

Estimate

Background

Estimate

2 4.845 7 5.11 867 0.12 0.05 0.05

9 2.91 11 3.14 887 0.11 0.03 0.03

3 3.943 12 4.26 888 0.11 0.04 0.04

2 4.845 5 5.31 901 0.11 0.05 0.05

1 4.292 2 4.845 928 0.11 0.04 0.05

1 4.292 7 5.11 978 0.10 0.04 0.05

1 4.292 5 5.31 1017 0.10 0.04 0.05

3 3.943 7 5.11 1065 0.09 0.04 0.05

3 3.943 5 5.31 1107 0.09 0.04 0.05

11 3.14 12 4.26 1115 0.09 0.03 0.04

9 2.91 10 4.05 1144 0.09 0.03 0.04

9 2.91 12 4.26 1203 0.08 0.03 0.04

5 5.31 6 8.028 1242 0.08 0.05 0.08

2 4.845 6 8.028 1361 0.07 0.05 0.08

2 4.845 4 8.687 1473 0.07 0.05 0.09

1 4.292 6 8.028 1537 0.07 0.04 0.08

1 4.292 4 8.687 1663 0.06 0.04 0.09

3 3.943 6 8.028 1673 0.06 0.04 0.08

3 3.943 4 8.687 1810 0.06 0.04 0.09

4 8.687 8 26.64 2520 0.04 0.09 0.27

6 8.028 8 26.64 2727 0.04 0.08 0.27

5 5.31 8 26.64 4122 0.02 0.05 0.27




Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

Intake

Estimate

Background

Estimate

7 5.11 8 26.64 4284 0.02 0.05 0.27

2 4.845 8 26.64 4518 0.02 0.05 0.27

1 4.292 8 26.64 5100 0.02 0.04 0.27

3 3.943 8 26.64 5551 0.02 0.04 0.27



Potential Adult Fish

Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

2 0.51 13 0.04 67 1.48

5 0.44 13 0.04 78 1.28

2 0.51 10 0.05 88 1.13

2 0.51 3 0.06 96 1.04

5 0.44 10 0.05 103 0.97

8 0.28 13 0.04 120 0.83

2 0.51 7 0.07 121 0.83

2 0.51 11 0.08 124 0.81

5 0.44 7 0.07 141 0.71

5 0.44 11 0.08 144 0.69

2 0.51 6 0.10 158 0.63

8 0.28 10 0.05 158 0.63

5 0.44 6 0.10 184 0.54

9 0.18 13 0.04 195 0.51

2 0.51 4 0.12 195 0.51

12 0.17 13 0.04 195 0.51

8 0.28 11 0.08 222 0.45

9 0.18 10 0.05 256 0.39

2 0.51 12 0.17 283 0.35

4 0.12 13 0.04 284 0.35

2 0.51 9 0.18 284 0.35




Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

5 0.44 12 0.17 330 0.30

5 0.44 9 0.18 331 0.30

6 0.10 13 0.04 351 0.29

9 0.18 11 0.08 359 0.28

4 0.12 10 0.05 372 0.27

11 0.08 13 0.04 446 0.22

7 0.07 13 0.04 458 0.22

2 0.51 8 0.28 460 0.22

6 0.10 10 0.05 460 0.22

8 0.28 12 0.17 506 0.20

8 0.28 9 0.18 508 0.20

4 0.12 7 0.07 509 0.20

4 0.12 11 0.08 522 0.19

5 0.44 8 0.28 535 0.19

3 0.06 13 0.04 577 0.17

7 0.07 10 0.05 601 0.17

10 0.05 13 0.04 626 0.16

6 0.10 7 0.07 629 0.16

6 0.10 11 0.08 645 0.15

4 0.12 6 0.10 665 0.15

1 0.05 13 0.04 674 0.15

2 0.51 5 0.44 706 0.14




Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

3 0.06 10 0.05 757 0.13

9 0.18 12 0.17 819 0.12

13 0.04 13 0.04 822 0.12

1 0.05 1 0.05 822 0.12

3 0.06 3 0.06 822 0.12

7 0.07 7 0.07 822 0.12

6 0.10 6 0.10 822 0.12

4 0.12 4 0.12 822 0.12

12 0.17 12 0.17 822 0.12

9 0.18 9 0.18 822 0.12

8 0.28 8 0.28 822 0.12

5 0.44 5 0.44 822 0.12

10 0.05 10 0.05 822 0.12

11 0.08 11 0.08 822 0.12

2 0.51 2 0.51 822 0.12

7 0.07 11 0.08 843 0.12

1 0.05 10 0.05 885 0.11

1 0.05 3 0.06 960 0.10

3 0.06 7 0.07 1035 0.10

3 0.06 11 0.08 1062 0.09

10 0.05 11 0.08 1152 0.09

4 0.12 12 0.17 1193 0.08




Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

4 0.12 9 0.18 1196 0.08

1 0.05 7 0.07 1209 0.08

1 0.05 11 0.08 1240 0.08

3 0.06 6 0.10 1352 0.07

6 0.10 12 0.17 1475 0.07

6 0.10 9 0.18 1479 0.07

1 0.05 6 0.10 1579 0.06

3 0.06 4 0.12 1671 0.06

11 0.08 12 0.17 1878 0.05

7 0.07 12 0.17 1926 0.05

7 0.07 9 0.18 1931 0.05

4 0.12 8 0.28 1936 0.05

1 0.05 4 0.12 1952 0.05

6 0.10 8 0.28 2394 0.04

3 0.06 12 0.17 2426 0.04

3 0.06 9 0.18 2433 0.04

10 0.05 12 0.17 2632 0.04

1 0.05 12 0.17 2834 0.04

1 0.05 9 0.18 2842 0.04

4 0.12 5 0.44 2972 0.03

7 0.07 8 0.28 3126 0.03

3 0.06 8 0.28 3938 0.03




Intake

Station

Intake

Abundance

Background

Station

Background

Abundance

Esig %

Entrained

1 0.05 8 0.28 4600 0.02

3 0.06 5 0.44 6045 0.02

1 0.05 5 0.44 7061 0.01

1 0.05 2 0.51 8219 0.01

ecoelectrica plankton and entrainment analysis (2)

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