Medical Exam-Biology  Biotechnology  Practice Q & A

1. A dividing and undifferentiated mass of cells is called

a. Callus

b. Embryo

c. Explant

d. Zygote

2. agarose extracted from sea weeds finds use in

a. Gel electrophoresis

b. Spectrophotometry

c. Tissue culture

d. PCR

3. In nucleoside, nitrogen base is attached to pentose sugar at

a. Carbon – 1 of pentose sugar

b. Carbon – 2 of pentose sugar

c. Carbon – 4 of pentose sugar

d. Carbon – 5 of pentose sugar

4. Continuous addition of sugars in fed batch fermentation is done to

a. Degrade sewage

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b. Produce methane

c. Obtain antibiotics

d. Purify enzymes

5. Which purine is found in RNA?

a. Guanine

b. Cytosine

c. Thymine

d. Uracil

6. DNA is found in

a. Chromosomes

b. Plastids

c. Mitochondria

d. All of the above

7. Dolly, The first cloned mammal, was produced by

a. Parthenogenesis

b. Artificial fertilization

c. Replacement of zygote nucleus by somatic nucleus

d. in vitro fertilization

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8. The figure below is the diagrammatic representation of the E.coli vector pBR 322. Which one of the

given options correctly identifies its certain component(s)?

a. Hind III, EcoRI-selectable markers

b. ampR, tetR-antibiotic resistance genes

c. ori-original restriction enzyme

d. rop-reduced osmotic pressure

9. embryo culture is used for

a. Establishing suspension culture

b. Recovery of inter specific hybrids

c. Somatic hybridization

d. Haploid production

10. genetic Engineering is

a. plastic surgery

b. addition or removal of genes

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c. study of extranuclear genes

d. all of the above

11. DNA fingerprinting was discovered by

a. James Watson

b. Alec Jeffreys

c. Frederick Fanger

d. Hargobind Khorana

12. genomics is the study of

a. Genes in general

b. Human genes

c. Genomes in general

d. Human genome

13. Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands.

What is so special shown in it?5 ______ GAATTC ________ 3 3 ________CTTAAG ________ 5

a. Palindromic sequence of base pairs

b. Replication completed

c. Deletion mutation

d. Start codon at the 5 end

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14. Maximum number of existing transgenic animals is of

a. Pig

b. Fish

c. Mice

d. Cow

15. In recombinant DNA technology a plasmid vector is cleaved by

a. Modified DNA ligase

b. A heated alkaline solution

c. The same enzyme that cleave the donor DNA

d. The different enzyme other than that cleave the donor DNA

16. Distance between two strands of DNA is

a. 34 Å

b. 20 Å

c. 3.4 Å

d. 340 Å

17. The first clinical gene therapy was given for treating?

a. Rheumatoid arthritis

b. Chicken pox

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c. Diabetes mellitus

d. Adenosine deaminase deficiency

18. One of the following processes involves southern blotting technique

a. rDNA technology

b. DNA finger printing

c. production of transgenic plants

d. formation of suspension culture

19. Plant tissue culture technique is a redefined method of ________

a. Hybridization

b. Vegetative propagation

c. Asexual reproduction

d. selection

20. Restriction endonucleases are used in genetic engineering and they

a. cut DNA at various sites

b. join DNA segments

c. cut RNA at specific sites

d. cut DNA at specific sites

21. Advancement in genetic engineering has been possible due to the discovery of

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a. Oncogenes

b. Transposons

c. Restriction endonuclease

d. Exonucleases

22. Functional unit of gene that specifies synthesis of one polypeptide is

a. Codon

b. Cistron

c. Recon

d. Muton

23. Restriction endonucleases are used in genetic engineering, because

a. they can degrade harmful proteins

b. they can join DNA fragments

c. they can cut DNA at specific base sequences

d. they can cut DNA at variable sites

24. Somatic hybridization is achieved through

a. Grafting

b. Protoplast fusion

c. Conjugation

d. Recombinant DNA technology

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25. Structure involved in genetic engineering is

a. Plasmid

b. Codon

c. Plastid

d. Scissors

26. The first hormone artificially produced by bacteria is

a. insulin

b. thyroxin

c. adrenalin

d. testosterone

27. The first transgenic crop was

a. Pea

b. Tobacco

c. Flax

d. Cotton

28. The most common substrate used in distilleries for the production of ethanol is

a. Molasses

b. Corn meal

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c. Soya meal

d. Ground gram

29. The nitrogen base which is absent in RNA is

a. Adenine

b. Cytosine

c. Guanine

d. Thymine

30. The plant Brassica napus has been modified to produce ______.

a. Vitamin A

b. hirudin

c. lysine rich seeds

d. insulin

31. The specific triplet of nitrogen bases on the transfer RNA is called

a. Codon

b. Anticodon

c. Genetic code

d. none of the above

32. The term gene was coined by

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a. Mc Clintock

b. Morgan

c. Johannsen

d. De Duve

33. The transgenic animals are those which have

a. foreign DNA in some of their cells

b. foreign DNA in all of their cells

c. foreign RNA in all of their cells

d. Both (A) and (C)

34. The two strands of DNA are held together by bonds of

a. Nitrogen

b. Oxygen

c. Hydrogen

d. Carbon

35. There is a restriction endonuclease called EcoRI. What does "co" part in it stand for?

a. coli

b. colon

c. coelom

d. coenzyme

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36. Cry genes or Bt genes are obtained from

a. Cotton pest

b. Tobacco plant

c. Bacillus thuringiensis

d. E. coli

37. Which enzyme is useful in genetic engineering?

a. DNAase

b. Amylase

c. Lipase

d. Restriction endonuclease

38. Which is related to genetic engineering?

a. Plastid

b. Plasmid

c. Heterosis

d. Mutation

39. Which of the following enzyme joints the okazaki fragments?

a. DNA polymerase

b. DNA ligase

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c. Helicase

d. Restriction endonuclease

40. Which of the following features should be present in vector?

a. It should replicate autonomously

b. It should have its own origin of replication

c. It should possess restriction enzyme cleavage sites

d. All of these

41. Which of the following is a genetic vector?

a. Plasmid

b. Phage

c. Cosmid

d. All of these

42. Which of the following is related to genetic engineering?

a. Mitochondrion

b. Golgi apparatus

c. Lysosome

d. Plasmid

43. Which of the following is used in Biowar?

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a. A pathogen

b. Toxin from a pathogen

c. A delivery system for the bioweapon agent

d. All of these

44. meristem culture is practiced in horticulture to get

a. Slow-growing callus

b. Somaclonal variation

c. Haploids

d. Virus-free plants

45. Which one is found only in RNA?

a. Cytosine

b. Adenine

c. Uracil

d. Guanine

46. Which one is a true statement regarding DNA polymerase used in PCR?

a. It is isolated from a virus

b. It remains active at high temperature

c. It is used to ligate introduced DNA in recipient cells

d. It serves as a selectable marker

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47. plasmid has been used as vector because :

a. Both ends show replication

b. It can move between prokaryotic and eukaryotic cells

c. It is circular DNA which have capacity to join to eukaryotic DNA

d. It has antibiotic resistance gene

48. Which part would be most suitable for raising virus-free plants for micropropagation?

a. Meristem

b. Node

c. Bark

d. Vascular tissue

49. For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of

a. Silicon or Platinum

b. Gold or Tungsten

c. Silver or Platinum

d. Platinum or Zinc

50. The enzymes required to obtain wall-free / naked protoplasts are

a. Cellulase and proteinase

b. Cellulase and pectinase

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c. Cellulase and amylase

d. Amylase and pectinase

Answer Keys

1. a 2. a 3. a 4. d 5. a

6. d 7. c 8. b 9. b 10. b

11. b 12. c 13. a 14. c 15. c

16. b 17. d 18. b 19. b 20. d

21. c 22. b 23. c 24. b 25. a

26. a 27. b 28. a 29. d 30. b

31. b 32. c 33. b 34. c 35. a

36. c 37. d 38. b 39. b 40. d

41. d 42. d 43. d 44. d 45. c

46. b 47. c 48. a 49. b 50. b

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