EASY-Spray technologyPlug-and-spray with state-of-the-art performance

Effortless nano electrospray ionization

2 | EASY-Spray Technology

EASY-Spray source

Integrated designNano-flow LC-MS relies critically on perfect

connections between several pieces of nano-bore

tubing and high-voltage electrodes for the spray

ionization. Thermo Scientific™ EASY-Spray™ technology

provides an integrated and temperature-controlled

column-emitter design using a single Thermo Scientific™

Dionex™ nanoViper™ connection between the LC and the

EASY-Spray source to remove the most error-prone and

difficult connections.

Plug-and-spray

nano-electrospray

ionization combined with

industry-proven columns:

the Thermo Scientific

EASY-Spray.

2 | EASY-Spray Technology Thermo Scientific | 3

More than ever beforeThermo Scientific proteomics solutions are designed

to give you more than ever before:

more throughput, more sensitivity, more

separation power and more ease of use.

“The optimization and reproducibility

achievable by years of nanospray experience

are built into the device. Heated

UHPLC 50 cm column chromatography was

not easy until now!”

— Paul Taylor, PhD Hospital for Sick Children,

Toronto, Canada.

Thermo Scientific™ EASY-nLC 1200 system coupled with Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ mass spectrometer

4 | EASY-Spray Technology

Plug-and-spray nano-electrospray ionization

Forward/backward adjustment knob

Simply press until it clicks into place

Classic connection

nanoViper connection

The EASY-Spray columns are high-tech assemblies that are carefully

manufactured to the highest standards in nano-flow chromatography.

Integrated ZDV union

Spray tipIntegrated column heating (30-60 °C operating range)

The metal cylinder provides a trouble-free high-voltage connection from the source

to the eluent

nanoViper™ fitting

Precision positioned glass emitter with uniform ID and untapered flow path

Circular design allows column to be coiled up, accommodating

long columns.

4 | EASY-Spray Technology Thermo Scientific | 5

Power and sensor cable for

EASY-Spray columns

Temperature control display

Forward/backward adjustment knob

Excellent spray stability

Sample: Singly charged polytyrosine peptide

MS: Thermo Scientific LTQ Velos

Source setup: EASY-Spray source direct infusion emitter

Simply press until it clicks into place

20000000

18000000

16000000

14000000

12000000

10000000

8000000

6000000

4000000

2000000

00 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30

Inte

nsity

Time (min)

Camera provides light and a

convenient, magnified view

of the emitter tip

6 | EASY-Spray Technology

State-of-the-art performance Achieving state-of-the-art performance from nano-flowLS-MS was never easier: simply plug-and-spray

EASY-Spray delivers performance, reproducibility and reliable results.

Outstanding reproducibility

7 BSA runs on a Thermo Scientific TSQ Vantage triple stage

quadrupole mass spectrometer. Retention time precision

less than 0.2% RSD.

Courtesy of Paul Taylor and Thomas Kislinger, Hospital for Sick Children, Toronto, Canada.

Reliable results every time

EASY-Spray provides stable performance over time to match

today’s requirements for proteomics researchers.

5 10 15 20 25 30 35 40 45 50 55 60 65Minutes

020406080

1000

20406080

100

020406080

100

Rela

tive

Abun

danc

e

020406080

10034.36

575.279222.16688.8204 38.55

901.463917.21

447.7194

46.03672.401115.09

410.213626.93

514.297830.84

629.3431 799.422245.79581.335910.64

483.255851.11

799.422758.44

801.753560.59

1104.25188.98514.7664

34.58575.2802

17.55447.7194 22.46

688.8212 40.54870.4832

15.41410.2140

13.12401.2454 46.21

672.402131.92

551.782027.13

514.2982 51.01799.423046.01

581.337312.05415.7356 51.76

590.316358.61

801.7560

34.05575.2821 39.93

870.485117.11447.7207

45.64672.4036

12.77401.2462

21.93688.8231

31.38551.7839

29.19528.2762

50.37799.424612.14

452.705745.44

581.338451.03

590.317357.99

801.757110.86

483.257760.14

1104.2584

34.46575.2799

12.97401.2449

17.39447.7197 22.33

688.821340.40

870.4829 46.09672.4016

26.98514.2983

31.74551.7820

12.30452.7043

50.79799.4229

45.52652.3970

11.10420.7693

51.57590.3154

8.82430.2198

58.36801.7546

Injection #1

Injection #15

Injection #30

Injection #45

Selected base peak chromatograms from 500 ng samples of E. coli digest analyzed on a Velos Pro Orbitrap instrument with over 45 repeat runs. A 50 μm ID × 15 cm column was used for the separation. The gradient was 5% B to 30% B in 60 min at 300 nL/min. The column heater was set at 35 °C.

Column: EASY-Spray ES901 (15 cm × 50 µm ID Thermo Scientific™ Acclaim™ PepMap™ RSLC C18, 2 µm)Peak width A: 2.5s FWHMPeak width B: 3.0s FWHM Courtesy of Karl Mechtler, Institute of Molecular Pathology (IMP), Vienna, Austria.

The combined column/sprayer fitted with nanoViper fittings eliminate dead volumes. The result: Ultra-sharp peaks (2.5-3.0 s full width half maximum) that maximize peptide coverage.

“Right off the shelf, with absolutely no optimization, the first injectiongave me beautiful peaks with FWHM times of 2.5-3 seconds!”

— Duncan Smith, PhD Paterson Institute for Cancer Research, Manchester, England.

Thermo Scientific | 76 | EASY-Spray Technology

RT min

# of injection40

45

50

55

60

0 5 10 15 20 25

Room Temp35 ⁰C45 ⁰C55 ⁰C

Temperature control Column temperature control

immediately before the MS inlet increases

run to run reproducibility and allows

the use of even longer columns and/

or smaller particle sizes since elevated

temperatures lower eluent viscosity and

reduce the overall backpressure.

Amazing peak capacity The entire assembly is designed for

heavy use so you can utilize the full

pressure range of your UHPLC front-

end (up to 1000 bar) and benefit from

small particles and long columns.

Longer columns and longer gradients

both contribute to more identifications.

RT reproducibility of peptide EAVNQVIALLDSGALR from 500 ng E.coli complex sample for 20 repeat runs in different temperature ranges.

4,087 unique proteins were identified from a single HPLC separation of a 1000 ng human cell digest with a 50 cm EASY-Spray column.

0 20 40 60 80 100 120 140 160 180 200 220 240 260Minutes

0

10

20

30

40

50

60

70

80

90

100

Rela

tive

Abun

danc

e

10.17414.7132

25.36431.2003 145.65

495.291462.37576.2874 122.88

472.7680112.49590.8126

182.62655.853883.78

554.285841.51415.7122

104.52513.306981.70

656.2866

238.66872.4122184.82

839.4651151.64757.3942

180.05697.3755

225.06966.5861161.02

799.8738 198.38851.4542

216.45595.3321

254.71932.7183

0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80

Time (min)

0

20

40

60

80

100

33.29575.2820

39.35870.4839

21.27688.822311.71

601.2952 29.75629.3451

44.84672.4033

50.22799.4244

10.88452.7047

53.75759.4297

57.75801.7576

7.84508.2492 62.10

964.057771.10

415.213573.38

488.3965

Column 3

0

20

40

60

80

100

Rela

tive

Abun

danc

e

33.68575.2828

21.70688.8232 38.14

901.4670 45.13672.4035

15.89629.837312.23

601.296025.99

514.299949.68

799.4254

10.86415.7364 57.57

801.7568 61.74964.0568

71.12415.2140

75.03469.3158

Column 2

0

20

40

60

80

100

33.14575.2822

21.09688.8206

36.36740.3671

50.17799.423645.43

672.402227.24

565.791415.96

447.720611.41601.2949

58.02801.7542

9.03531.2291 62.70

964.054972.79

532.3857

Column 1 Excellent inter-column reproducibility Column-to-column reproducibility

is extremely important in

proteomics, particularly when

running big experiments or multi-site

collaborations. The exacting assem-

bly precision of EASY-Spray columns

ensures class leading performance on

these parameters.

Find out more at thermofisher.com/EasySpray

©2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries. This information is presented as an example of the capabilities of Thermo Fisher Scientific products. It is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representatives for details. BR70044-EN 0820

Full compatibility EASY-Spray source is fully compatible with the followingThermo Scientific mass spectrometers and columns:

Thermo Scientific™ EASY-Spray™ source

Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ mass

spectrometer

Thermo Scientific™ Q Exactive™ HF hybrid quadrupole-Orbitrap mass

spectrometer

EASY-nLC 1200 systemOperational simplicity and excellent

performance

Thermo Scientific™ Dionex™ UltiMate™ 3000 RSLCnano system

Versatility and unsurpassed precision

Thermo Scientific™ EASY-Spray™ column

Thermo Scientific™ TSQ Quantiva™ triple quadrupole mass

spectrometer