Wireless highly selective lactic acid biosensor based on a nanostructured catalyst

by

About§  Robo-Med Ltd.

§  Main area of activity:

ü  Development of object recognit ion methods & 3D reconstruction;

ü  Development of rehabilitation and training systems for the musculoskeletal system.

ü  Development of non-invasive biosensors for real-time monitoring of body’s vital signals;

ProblemUntimely or inaccurate diagnosis ->

DISABILITY or DEATH

In Russia only*: > 1,3 million deaths from cardio-vascular diseases

> 450 000 cases of stroke annually

> 800 000 limb injuries annually

> 1 million cerebral palsy handicaps

Traditional blood analysis for determining lactic acid doesn’t work in real-time application

* Data provided by Rosstat, University of Lausanne

Solution

§  "Prussian Blue“ is used as the catalyst with polymer immobilization of the organic enzyme;

§  “Prussian Blue” is nanostructred on the electrode surface, which greatly improves the biosensor sensitivity and selectivity;

§  Mechatronic, microfluidic design of the sweat collector with micropumps analysis and wireless data transmission to a smartphone;

§  The biosensor can be used as part of a at-home muscoskeletal rehabilitation system for control of the critical levels of physical activity.

Development of a real-time wireless non-invasive lactic acid biosensor:

Competitive Advantages: Non-invasive Autonomous Ease of use

Personal health management

Nanotech component

Reproducibility Mixing enzymes with waterproof polymers

Homogeneous nanopolymeric membranes

Preparation of the true polymer solutions

Nanotechnology of exposing enzymes to organic solvents

Biosensor structure:

1. An electrode consisting of layers of silver, graphite and insulator is coated with a layer of Prussian Blue using the nanostructuring method.

2. The Prussian Blue layer is covered with a thin layer of true polymer solution with enzyme molecules and as a result we get a homegenous nanopolymer membrane which is less than 100 nm thick.

3. Enzyme molecule diameter is about 7 nm, substrate molecules - 0,8 nm.

Nanotech component

Ele

ctro

de

Nanopolymer membrane

Lactic acid

Piruvate

Lactate oxidase

Lactate oxidase

Prussian Blue

Product characteristics §  Lactate, cardio, temperature non-invasive sensors

§  Real-time flow system (application: sports, rehab)

§  Monitoring service (cloud subscription)§  Control of vital signals & critical physical loads, history§  Teleconsultations and social networks§  Rehabilitation & training system§  Flow sensor + subscription§  3D reconstruction system with biomechanics real-time analysis§  Rehab treadmill system (optional)

Rehab system (sensor + AKS + subscription)

Subscription

Analysis of kinematic signatures (AKS)

Market Structure

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 Rehab  market  

$29.6  billion  (2014)  $38.8  billion  (2018)  

 

MHealth  $1.5  billion  (2012)    $21.5  billion  (2018)  

Ensimus  (No-­‐risk  PHM*)  

Mo:on  Capture  $4.8  billion  (2016)  $10  billion  (2019)  Home  telemedicine  

$611  million  (2014)  $4548  million  (2018)  

Data sources: RBC, РАТМ, ACRM (American Congress of Rehab Medicine)

*Persnal Health Management

Target markets

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Home use: §  Injury and heart stroke rehab; §  Physical load control, monitoring progress

for athletes and fitness enthusiasts.

Professional use: §  High performance sports §  Transplant surgery §  Newborn screening for genetic disorder

- cystic fibrosis

Marketing & Sales

   

Cer:fied  suppliers,  integrators,  tenders  and  auc:ons  

   

Commercial  fitness  equipment  resellers,  sport  medicine  and  rehab  centers/

clinics  

Distrib

u:on

 chan

nels  

Stratgey    B2G:    Working  with  departmental  health  

organiza:ons  

 B2В:    Par:cipa:on  in  expos,  trade  fairs,  associa:ons,  

communi:es    

 E-­‐shops.  Direct  sales  

network  

B2С:  social  marke:ng,  populariza:on,  

par:cipa:on  and  crea:on  of  health  context    based  communi:es  

Main market Secondary market

Partner  network

Project status & break-even

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2014 Demo version

Partners

16

Pre-Seed Investment Fund

North-West Center for Technology Transfer

Saint-Petersburg Administration

Contact us

Anton Panfilov

Phone: +7(926) 925-3763 Skype: AVP1962

Email: panfilov@tradition.ru http://www.robo-med.ru

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