Slide 1

Duke University, Fitzpatrick Institute for Photonics BIOS Lab, Department of Biomedical Engineering Advances in time-resolved and spectroscopic quantitative phase microscopy Duke University Department of Biomedical Engineering Matthew Rinehart Preliminary Examination 7/24/2012

Slide 2

Outline Summary of course work Peer-reviewed publications Motivation & introduction Preliminary results QPM technology QPM applications QPS Research aims & timeline

Slide 3

Summary of course work TermCourseCourse TitleInstructorUnits Fall 2008PATH 225 Intro to Systemic HistologyL. Hale2 BME 265Advanced OpticsJ. Kim3 BME 233Modern Diagnostic Imaging Systems J. MacFall3 Spring 2009 ECE 299Imaging & SpectroscopyD. Brady3 PATH 250 General PathologyL. Hale4 Fall 2009ECE 299Holography & Coherent ImagingD. Brady3 PHY 230Math Methods in PhysicsA. Kotwal3 Spring 2010 BME 265Image ProcessingS. Farsiu3 BIO 154Fundamentals of NeuroscienceS. Bilbo3 Fall 2010ECE 376Lens DesignD. Brady3 Spring 2011 ECE 399Independent Study: Broadband Diffraction Phase Microtomography D. Brady3 Fall 2011BME 234Modern MicroscopyA. Wax3 BME 362Invention to ApplicationB. Myers3 Spring 2012 BME 362Invention to ApplicationB. Myers3 RequirementsUnits Advanced Math3 Life Sciences8 ECE Masters Courses 12 Total:42 RCR Hours8 (of 12) TermCourseCourse TitleInstructor Fall 2009BME 171Intro to Signals & SystemsJ. Izatt Fall 2010BME 171Intro to Signals & SystemsJ. Izatt Teaching Experience

Slide 4

System filters & fluorophores Bandpass filter Chroma Technology notch filter > OD5 at both 376nm and 632.8nm < 95% transmission 420-620nm, 650-750nm Fluorophore choice Nucleic acid stain (Invitrogen Hoescht 34580) Mitochondria stain (Invitrogen MitoTracker Deep Red)

Slide 62

Fluorescence imaging channel We have measured fluorescence of films and active agents (UC-781, CSIC) Fluorescence can potentially be used to simultaneously investigate the structure of these films or the rate of diffusion of active agents Targeted Delivery of PSC-RANTES for HIV-1 Prevention using Biodegradable Nanoparticles. Ham, et al. Pharmaceutical Research, Vol. 26, No. 3, March 2009

Slide 63

System filters & fluorophores Bandpass filter Chroma Technology notch filter > OD5 at both 376nm and 632.8nm < 95% transmission 420-620nm, 650-750nm Fluorophore choice Nucleic acid stain (Invitrogen Hoescht 34580) Mitochondria stain (Invitrogen MitoTracker Deep Red)

Slide 64

Coregistered fluorescence & quantitative phase 10m Fluorescence Map Red = Mitochondria Blue = Nuclei Quantitative phase profile Phase Instantaneous Differential (PID) Blue= PID + Red = PID -

Slide 65

Proposed Work Develop QPM instrumentation & techniques Develop QPS instrumentation & techniques Apply QPM to dynamic systems Apply QPS for molecular specificity Build InCh Microscope Develop robust & standardized assay for film dissolution Improve Illumination Add binocular display Use ratiometric imaging & photothermal Image microbicidal films & RBCs with molecular specificity