Drosophila melanogaster

Objectives

• Understand the life cycle of Drosophila melanogaster

• Use ether to anesthesize Drosophila… without killing them

• Distinguish males and females

• Identify mutations

Introduction

• Order: Diptera • Family: Drosophilidae • Genus: Drosophila • Species: melanogaster

Approximately 1,500 species of Drosophila

Drosophila as a model organism

• Small size

• Readily available

• Short generation time

• Many offspring

• Genetics: 4 pairs of chromosomes

Proboscis Extension Courtship Behavior Sleep Behavior

• Life cycle stages – Egg – Larva – Pupa – Adult

8 days

6 days

0 L P A

molting

Females can lay eggs the day after emerging from pupa

Life cycle can be temperature dependent

Wild-type Drosophila melanogaster

Peine sexual

Female Male

Separate males and females

Hembra Macho

Materiales: Medio de cultivo

• Guineo • Fórmula instantánea

Anestesia Éter

En el laboratorio utilizaremos éter para dormir las moscas, pero además se puede utilizar CO2

Mutants

• Many

• Affected characteristics:

– Eye color and form

– Body color

– Bristle size and form

– Wing size and form

– Absence of wings

– Behavior

Mutants