droplet gradient array for parallel experimentation via microfluidic device lindsay pickett faculty...
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Droplet Gradient Array For Parallel Experimentation via Microfluidic Device
Droplet Gradient Array For Parallel Experimentation via Microfluidic Device
Lindsay PickettFaculty Mentor: Abraham P. Lee
Graduate Mentor: Rob LinIM-SURE 2006
Lindsay PickettFaculty Mentor: Abraham P. Lee
Graduate Mentor: Rob LinIM-SURE 2006
Outline of DiscussionOutline of Discussion
IntroductionGoals
ProcessResultsIssues
SolutionsFuture Direction
IntroductionGoals
ProcessResultsIssues
SolutionsFuture Direction
IntroductionMicrofluidic Devices
IntroductionMicrofluidic Devices
• Widely applicable- engineering, biology, chemistry– Research tools– Commercial applications
• Advantages: – microscale fluid physics– less reagent & shorter Rxn time – parallel operations – lab space conservation,– easy/cheap fabrication
• Widely applicable- engineering, biology, chemistry– Research tools– Commercial applications
• Advantages: – microscale fluid physics– less reagent & shorter Rxn time – parallel operations – lab space conservation,– easy/cheap fabrication
Project GoalProject Goal
Droplet Gradient Array
Ismagilov Laboratory– Thorough characterization– Control over droplet properties
• Droplet volume•Gradient steps
– Versatility of device for multiple applications
Droplet Gradient Array
Ismagilov Laboratory– Thorough characterization– Control over droplet properties
• Droplet volume•Gradient steps
– Versatility of device for multiple applications
Project OutlineProject Outline
Process: Device designFabrication TestingExternal valve
systemComputer-
mediated step gradient
Characterization
Process: Device designFabrication TestingExternal valve
systemComputer-
mediated step gradient
Characterization
QuickTime™ and a decompressor
are needed to see this picture.
Device DesignDevice Design
Outlet reservoir
Reagent Inlets
Oil Inlet
Shearing JunctionArray Observation Region
Process: Device designProcess: Device design
12 µm
7.5 µm
70/30 µm 50/50 µm 12/7.5 µm12/7.5 µm
Process: Experimental SetupProcess: Experimental Setup
Results:Testing
Results:Testing
• Top Aq. Inlet: DI (variable rate)
• Bottom Aq. Inlet: DI + dye (const. rate)
• Oil inlet: Silicon oil (const. rate 2µL/min)
*Droplet content control*
• Top Aq. Inlet: DI (variable rate)
• Bottom Aq. Inlet: DI + dye (const. rate)
• Oil inlet: Silicon oil (const. rate 2µL/min)
*Droplet content control*
0.80 vs 0.70 µL/min
0.95 vs 0.70 µL/min
1.25 vs 0.70 µL/min
QuickTime™ and a decompressor
are needed to see this picture.
Results: Array Capture (Colorcam)
Results: Array Capture (Colorcam)
• Array image capture external valves work• Not pictured instability introduced by valves
– Initializing system: backflow, fluctuation of flow rate– Over 20 min observation period: chaotic, random instability
• Array image capture external valves work• Not pictured instability introduced by valves
– Initializing system: backflow, fluctuation of flow rate– Over 20 min observation period: chaotic, random instability
Solutions…Solutions…
VALVES• Contacted vendor…
– no suitable suggestions
– different fluid route (Dr. Lee)
• Different External Valves• Quake pressure-
actuation Valves• 2-D valve for sufficient
freeze
VALVES• Contacted vendor…
– no suitable suggestions
– different fluid route (Dr. Lee)
• Different External Valves• Quake pressure-
actuation Valves• 2-D valve for sufficient
freeze
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
Other Solutions…Other Solutions…
*Fluid re-routing…*new filtration design… *input tips*Fluid re-routing…*new filtration design… *input tips
Future DirectionFuture Direction
• Valves/new designs• Computer-mediated
step gradient (Hyperterminal)
• Characterization via GFP and ImageJStep gradientDroplet volume
• Valves/new designs• Computer-mediated
step gradient (Hyperterminal)
• Characterization via GFP and ImageJStep gradientDroplet volume
AcknowledgementsAcknowledgements
• NSF and the IMSURE program• Abraham P. Lee (faculty mentor)• Rob Lin (grad. student mentor)• Lee laboratory team• Eckerd College Biochemistry Dept.
• NSF and the IMSURE program• Abraham P. Lee (faculty mentor)• Rob Lin (grad. student mentor)• Lee laboratory team• Eckerd College Biochemistry Dept.