dr steve pereira institute of hepatology ucl medical school & university college hospital
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International PSC Study Group Meeting, Oslo, 14-15 Jun 2010 Cholangiocarcinoma in PSC: Opportunities within a study group. Dr Steve Pereira Institute of Hepatology UCL Medical School & University College Hospital. Characterisation of serum proteins in biliary tract cancer, PSC and - PowerPoint PPT PresentationTRANSCRIPT
International PSC Study Group Meeting, Oslo, 14-15 Jun 2010
Cholangiocarcinoma in PSC: Opportunities within a study group
Dr Steve Pereira
Institute of HepatologyUCL Medical School
&University College Hospital
Characterisation of serum proteins in biliary tract cancer, PSC andIAC using 2D-DIGE and LC-MS/MS
Neomal S Sandanayake1,2,3, John Sinclair3, Fausto Andreola2, Michael H Chapman1,2, Stephane Camuzeaux3, George J Webster1,2, Ian D Norton4 , Ross C Smith5, John F Timms3, Stephen P Pereira1,2
1Department of Gastroenterology and Hepatology, University College London Hospital; 2Institute of Hepatology, University College London; 3Institute for Women's Health, University College London; 4Department of Gastroenterology, 5Department of Surgery, Royal North Shore Hospital, University of Sydney, Australia
Conclusions: Differentially expressed serum proteins were identified in BTC compared to PSC, IAC and healthy controls using serum immuno-affinity depletion, 2DDIGE and LC-MS/MS.
Proposal
Suspicious biliary strictures
ImagingBile / brushingsBloodTissue
Clinical follow for > 1 yr in patients without proven malignant diagnosis
Malignancy Benign disease
Biobank
Patients: >18 yrs Benign or suspected or proven malignant PB diseaseWritten informed consent
Samples: Biliary brushings and bile - in storage buffer (provided)
Blood
- NIH SOPs- inverted 5 times, 1 hour clot at room temp, centrifuged 2200rpm at 4°C, serum aliquoted into 1.5ml cryovials, -80° C
Tissue samples - snap-frozen in liquid N2 and stored at -80°C- FFPE
TRANSBIL: TRANSlational research in BILiary tract cancer
+
Proposal
Pre-clinical samples202,000 women
- annual phlebotomy
270 individuals developed pancreatic cancer
54 individuals developed biliary tract cancer
UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS) serum bank at UCL
Resource
Cholangiocarcinoma in PSCOpportunities for trials?
• Surgery / OLT
• Stents
• Radiotherapy / Brachytherapy
• Chemoradiation
• Chemotherapy
• PDT
April 6 2010