dr. pablo gonzález-alanis - ag.arizona.edus/pablofoodsafety.pdf · more than 79,000 sq. km. of...
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Dr. Pablo González-Alanis
More than 79,000 sq. Km.
of territory, 370 Km of
border with USA, and 420
Km of litoral front to Gulf of
Mexico, plus 536 sq Km. of
rivers and lagoons.
Aquaculture activities
Increasing Support
From Industry,
Goverment and NGO s
• Since 2006 epidemics of gastro intestinal
pathogens were traced to consumption of
fresh vegetables.
• “Some cases” were related to the
contamination from human or animal wastes
applied through water irrigation.
• As we encourage more organic farming
methods and re-use of composted wastes
and effluent waters from animal operations to
irrigate field and crops, we increase the
opportunities for these type of contamination.
• We propose that complementary laboratory
and field studies be performed to examine
the bacteria flora typically encountered in
indoor and out door aquaculture operations.
The aquaculture program at the UAT developed aquaculture system adapted
from the US, Indonesia, Italy, Korea, Egypt and other Central American
Countries.
Information about health and environmental issues have been monitored and
recorded in order to report the presence of human bacterial pathogens in the
aquaculture systems water and potential contamination.
This research evaluated water and plants samples from both systems over 2
years period to determine:
• The presence of total and fecal coliforms, salmonella and enterococci.
• And if the UV treatment make a difference. And report the number of
organisms reduction.
Water and plants samples were collected from indoors (UAT) and outdoors
systems (Conrado Castillo).
Fish and plants were grown during Summer, Fall and Winter at different intervals.
Standard methods NOM were used for presumptive analysis.
•Tests for Salmonella, E. coli and Enterococci were
negative.
•Water sample on each date and each system found total
and fecal coliforms in measurable numbers.
•UV treatment did significative difference compared with
non-treated.
INDOORS
Lettuce Spinach
Mean SD Mean SD
UV
RootsTOTAL COL 0.145 0.098
UV
RootsTOTAL COL 0.100 0.011
FECAL COL 0.095 0.085 FECAL COL 0.090 0.016
LeafsTOTAL COL 0.14 0.087
LeafsTOTAL COL 0.025 0.057
FECAL COL 0.075 0.034 FECAL COL 0.115 0.064
NT
RootsTOTAL COL 0.09 0.040
NT
RootsTOTAL COL 0.115 0.077
FECAL COL 0.105 0.075 FECAL COL 0.010 0.011
LeafsTOTAL COL 0.06 0.034
LeaftsTOTAL COL 0.105 0.010
FECAL COL 0.06 0.034 FECAL COL 0.155 0.086
TOTAL COLIFORMSp=.00000
ROOTS UV LEAFS UV ROOTS NT LEAFS NT
LETTUCCE
-2
-1
0
1
2
3
4
5
6
7
MP
N
FECAL COLIFORMSp=.00000
ROOTS UV LEAFS UV ROOTS NT LEAFS NT
LETTUCCE
-2
-1
0
1
2
3
4
5
6
MP
N
OUTDOORS
Lettuce Spinach
Mean SD Mean SD
1
UVRoots TOTAL COL 0.147
0.135
1UV Roots TOTAL COL 0.072
0.47FECAL COL 0.109 FECAL COL 0.083
Leafs TOTAL COL 0.154 Leafs TOTAL COL 0.098
FECAL COL 0.096 FECAL COL 0.089
2
NTRoots TOTAL COL 3.308
3.41
2NT Roots TOTAL COL 4.572
3.55FECAL COL 3.581 FECAL COL 3.864
Leafs TOTAL COL 4.371 Leafs TOTAL COL 3.793
FECAL COL 3.648 FECAL COL 3.439
OUTDOORS
WATER
TOTAL COLIFORMS
Mean SD Mean SD
4.5 3.985 5.5 5.044
Letucce Spinach
•BENEFITS AT THIS POINT
•Uv light reduces in numbers fecal and total coliforms and
can be an alternative to keep bacteria on safety levels for
food consumption.
•Integrated fish and plants culture can be accepted as
technology by people on regions with high levels of
margination or poverty.
•Family work groups have better production results than non
familiar work groups .
Dr. Pablo Gonzá[email protected]