downloadspro68-01 manual urin micro sop
TRANSCRIPT
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
1/19
Your Lab Name
Manual Urinalysis Microscopic Exam
Author: Penny Stevens
Document Number: Pro 68-0.1
Effective (or Post) Dte: ! "une #008
Document $ri%in
&S Army 'osit 'ei*eber%+ ,ermny
omny: NA
S/E Arove* by: 'ei*i
S/E omments: 2his *ocument is rovi*e* s n e3me ony. t must be revise* to ccurtey refectyour b4s secific rocesses n*or secific rotoco re5uirements. &sers re *irecte* to counterchecfcts 7hen consi*erin% their use in other ictions. f you hve ny 5uestions contct S/E.
oy 99999 Effective Dte: Dte
U. URINALYSIS
U.02. MANUAL URINALYSIS MICROSCOIC E!AM
U.2.". RINCILE#
1. outine &rinysis consists of both hysic n* chemic nyses to ssist hysicinsin the *i%nosis n* tretment of ren n* urinry trct *iseses n* in the *etectionof metboic or systemic *isese rocesses not *irecty rete* to the i*ney.
#. 2he microscoic e3mintion of the centrifu%e* urine se*iment incu*es the stu*y offorme* eements+ such s ;
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
2/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
&o ,
C. (2he secimen must be brou%ht to room temerture before testin%.)
?. -'e *ollo%in urine samples are no& sa&is*ac&ory *or &es&in#
?.1. Secimens receive* over t7o hours fter coection.
?.#. /isbee* smes.
?.=. mroery coecte* smes. or e3me+ urine smes 7ith reservtives+secimens coecte* in non-sterie continers+ or secimens coecte* incontiners 7ith so or *eter%ent resi*ues 7i not be ccete*.
?.>. BNS (Buntity Not Sufficient) - 2he recommen*e* minimum voume is 1# m4s.2he re5uire* minimum voume for microscoic e3mintion is 0.?0 m. n theevent tht ess thn C0.?0 m is receive*+ erform testin% on uncentrifu%e* urinen* comment s note* in section &.#.@.
?.?. n the event tht n unccetbe sme is receive*+ nother sme must bere5ueste*. Document ction ten in the S.
U.2.+. E/UIMEN- REA1EN-S
1. E5uiment:
1.1 ov System - ov si*es+ iettes tubes1.#. entrifu%e 7ith timer1.=. /icroscoe 7ith 103 n* >03 obFectives1.>. ens er or int free tissues1 ? h
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
3/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
run.(see above)
1.3. econstitute ne7 ov contros 7ith 40 mL Reaen& 1ra5e %a&erccor*in%to mnufcturer4s instructions.
1.=.1. After reconstitution+ serte contros into # m i5uots usin%*isosbe trnsfer iettes n* stic test tubes. ti%htyH
1.=.#. be ech stic test tube 7ith Normalor Abnormal 6o7a Con&rol8ot number+ e3irtion *te+ reconstitution *te+ n* initis of tech 7horeconstitutes contros.
1.=.=. Sme i5uots 7i be store* froGen t -#0. 2he sme i5uots re
stbe t this temerture for up &o *our (+) mon&'s.1.=.>. $ny one freeGeth7 cyce is ermitte*. Ai5uots must be t room
temerture before testin%. Do not use 7rmin% boc to th7.1.=.?. Sme i5uots re stbe for one hour t 1?-#0I or #> hours t #-8I.
1.4. Perform the ov contros in the sme mnner s tient smes. See section&.#.@ for the tient testin% roce*ure. ecor* the resuts on the &rinysisBuity ontro ;orsheets. See en*i3 = >.
1.5. A resuts must f into the e3ecte* rn%es+ 7hich re obtine* from themnufcturer4s insert for ech contro n* recor*e* on the to of the &rinysisBuity ontro ;orsheets.
1.6. f resuts re 7ithin e3ecte* rn%es+ rocee* 7ith tient testin%.
1.7. f B resuts re outsi*e of e3ecte* rn%es for contros:
1 @ 1 i h i i *iff fi * f i
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
4/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
1. emove the secimenscontros from the refri%ertor or freeGer+ s icbe+ n*
o7 them come to room temerture for t est =0 minutes before mi3in%.
#. Positivey i*entify the secimens usin% the tient nme n* *te of birth.
=. o% the sme on the testin% 7orsheet usin% the S %enerte* tient be+en*i3 6.
>. A** the S %enerte* be to the sme cu (not the sme i*). Any 5iscrepancymus& be in7es&ia&e5 be*ore processin &'e specimen.
?. /i3 tient urine sme by s7irin%. /i3 contro i5uots by invertin% sever timesto ensure homo%eneity of the contents.
6. f mcroscoic e3mintion urine chemistry testin% is or*ere* refer to &.01.1/nu &rinysis Distic 2estin% S$P for testin% n* reortin% roce*ures. 2hemicroscoic e3mintion is erforme* after *istic testin% uness the rovi*erre5uests other7ise.
@. f intest+ Acetest or ctotest is re5uire* s iste* beo7 or re5ueste* by the rovi*er+erform testin% on uncentrifu%e* urine rior to erformin% the microscoic e3m.efer to in*ivi*u confirmtory test S$Ps s iste* in the references.
8. 2he Clini&es& con*irma&ory &es& is re5uire* for all pe5ia&ric urine samples 7ithtients %e2 years ol5 oryouner+ re%r*ess of 7hether it 7s re5ueste* or not.See initest S$P &06.1.
!. 3ac;up &es&s an5
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
5/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
11.#. /icroscoic e3mintion re5ueste* by the tient4s hysicin.
11.=. &rine secimens receive* from Pe*itric inic.11.>. theteriGe* n* Surubic sirtion smes.11.?. &rine secimens receive* from Physic E3m inic.
1#. Pour 1#-m4s of secimen into sterie ov tube n* be the tube 7ith the Ssecimen be. f the tot secimen voume is 0.?0 m or ess+ *o not our thesme into ov tube. Perform testin% from the secimen cu s in*icte* in thefoo7in% stes.
1=. 2estin%
1=.1. Loumes %reter thn 0.?0 m
1=.1.1. Perform Distic+ initest+ Acetest+ or ctotest s re5uire* orre5ueste*.
1=.1.#. the ov sme n* centrifu%e t 1?00 rm for ? minutes.1=.1.=. emove the sme from the centrifu%e n* *iscr* the c.1=.1.>. f =K SSA testin% is re5uire* or re5ueste*+ erform testin% on the
suerntnt ccor*in% to the &.10.1. =K SSA S$P.1=.1.?. Decnt n* *iscr* the suerntnt 7hie retinin% the se*iment eet
in the conic ortion of the ov tube. 2here 7i be sm voumeof fui* retine* bove the se*iment. 2he tot voume retine* in theov system tube fter *ecntin% the suerntnt shou* bero3imtey 0.? m.
1=.1.6. &se ov *isosbe iette to resusen* the se*iment eet.1=.1.@. Procee* 7ith testin% in ste 1=.=.
1= # Loumes ess thn 0 ?0 m s7ir the sme in the secimen cu to mi3 7e
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
6/19
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
7/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
Jest None i%ht+ /o*erte or /ny. eort ny
bu**in% yest or hyhe seen usin%comments s note* beo7.
2richomons None e7+ /o*erte or /ny. /y ony bereorte* if motie.
#. f bu**in% yest or hyhe re resent+ ** the foo7in% cnne* comments:
#.1. O
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
8/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
?.#. ritic vues must be reorte* in ccor*nce 7ith the bortory critic vueoicy.
?.#.1. A critic microscoic se*iment must be verifie* by the bortorysuervisor before reortin% to the hysicinnurse n* before certifyin%resuts in the S.?.#.#. A resuts must be re* bc by the hysicinnurse n* thenotifiction re* bc *ocumente* in the S.
?.#.=. 2he thoo%ist must so be notifie* 7ithin # hours n* 7i*etermine if thoo%ist revie7 is re5uire*.
U.2.. ROCE:URAL NO-ES#
1. NORMAL C>ARAC-ERIS-ICS OB ->E URINE#2he yeo7 coor of the urineis *ue r%ey to the i%ment urochrome n* sm mounts of urobiin n* uroerythrin.Norm urine is essentiy cer+ n* the resence of rticute mtter in
uncentrifu%e* urine nee*s to be e3ine* microscoicy. Norm urine hs fint+romtic o*or of un*etermine* source.
#. Microscopic Se5imen
#.1.
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
9/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
Amorhous &rtes Amorhous
Phoshtes
ystine None
&ric Aci* 2rie Phoshtes 2yrosine
cium $3te Ammonium
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
10/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
?.11.#.#. Ba&&y cas&s# Are semitrnsrent or %rnur cyin*ers
continin% r%e hi%hy refrctive vcuoes or *roets. Lisibeft *roets re tri%yceri*es or choestero esters. 2hese recommony seen 7hen there is hevy roteinuri n* re feture of the nehrotic syn*rome.
?.11.#.=. Crys&al Cas&s# rystine incusion in semitrnsrent or%rnur cyin*er. 2hese csts in*icte *isosition of crysts inthe tubue or coectin% *uct.
?.11.=. Cas& imen&s#
?.11.=.1. >emolobin (3loo5) cas&s#Aers yeo7 to re* sometimesthe coor is very e n* *ifficut to interret. 2hese csts ressocite* 7ith %omerur *isese.
?.11.=.#. Myolobin cas&s#2hese cst re re*-bro7n in coor n* occur
7ith myo%obinuri foo7in% cute musce *m%e.
?.11.=.=. 3ilirubin an5 o&'er 5ru cas&s#. Cellular Cas&s#
?.11.>.1. Ery&'rocy&e (Re5 3loo5 Cell) cas&s# Semitrnsrent or%rnur cyin*ers continin% *istinct erythrocyte Disor*ers
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
11/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
center of the 7e cn cuse ir bubbes to form resutin% in sme *istortion
*urin% e3mintion.
U.2."0. AEN:ICES#
1. S$P Li*tion orm n* S$P hn%e ontro#. S$P Arov=. &rinysis Norm Buity ontro ;orsheet>. &rinysis Abnorm Buity ontro ;orsheet
?. Buity ontro Pre 2estin% ;orsheet6. Ptient esut orm@. &rine ryst Proerties
U.2."". REBERENCES#
1. Strnsin%er+ Susn .+ &rinysis n* .
#. 'ber+ /ery '.+ &rinry Se*iment: A 2e3tboo Ats+ Americn Society of inicPthoo%ist .
=. /utisti3 10 7ith S, Pc%e nsert+ . ov 2ro: 'umn &rinysis ontros Pc%e nsert+ 'ycor
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
12/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
Aen*i3 1
S$P LADA2$N
S$P NA/E:U.2. Manual Urinalysis Microscopic Exam
er n* secific tite n* rincie: yes noomments:
A necessry suies+ e5uiment+ n* mteris re iste*: yes noomments:
S$P is sufficienty *etie* to be un*erstoo* but not overy come3: yes noomments:
S$P te3t *e5utey *escribes rocessroce*ure: yes noomments:
S$P ccomishes urose: yes no
omments:
i * b (N 2i ) )
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
13/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
Aen*i3 #S$P APP$LA
S,NA2&E DA2E
PEPAE
BA $$DNA2$
A
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
14/19
Your Lab Name
Aen*i3 =Urinalysis Dipstick and Microscopic Normal Control Log
Normal Control Lot#: Expiration: Month/Year:
Dipst ick Lot# Expirat ion: Date in use: Supervisor Review:
Dipst ick Lot# Expirat ion: Date in use: Review Date:
Color/Clarity
Glucose Bilirubin Ketones Specific
Graity B lood p! "rotein Urobilin# Nitrate
Leu#$sterase
%BC/&pf 'BC/&pf Crystals Casts (ec&
)nitials
Date ccepta!le
Ran"e:
$
%
&
'
(
)
*
+
,
$
%
&
'
(
)
*
+$,
$
$$
$%
$&
$'
$(
$)
$*
$+
%,
%
Comments:
efer to S$Ps &.1.1 n* &.#.1 for &rine Distic n* /icroscoic B resutin% roce*ures
#?8=##@@=.*oc P%e 999 of 1! Effective: Dte
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
15/19
Your Lab Name
Aen*i3 >Urinalysis Dipstick and Microscopic *bnormal Control Log
!normal Control Lot#: Expiration: Month/Year:
Dipst ick Lot# Expirat ion: Date in use: Supervisor Review:
Dipst ick Lot# Expirat ion: Date in use: Review Date:
Color/Clarity
Glucose Bilirubin Ketones Specific
Graity B lood p! "rotein Urobilin# Nitrate
Leu#$sterase
%BC/&pf 'BC/&pf Crystals Casts (ec&
)nitials
Date ccepta!le
Ran"e:
$%
&
'
(
)
*
+
,
$
%
&
'
(
)
*
+
$,
$
$$
$%
$&
$'
$(
$)
$*
$+
%,
%
Comments:
efer to S$Ps &.1.1 n* &.#.1 for &rine Distic n* /icroscoic B resutin% roce*ures
#?8=##@@=.*oc P%e 999 of 1! Effective: Dte
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
16/19
Your Lab Name
Aen*i3 ?Buity ontro Pre 2estin% ;orsheet
Reaen& Curren& Lo&Resul&
Curren& Lo&Accep&able
Rane
Ne% Lo& Resul& Curren& Lo&Accep&able
Rane
Accep&able
e*
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
17/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
Aen*i3 6 - Ptient /nu &rinysis esut eort
Anyte Ptient esuts eference n%e
'yine sts P:
0-1 1-= =-? ?-10 10-#? #?-?0?0
0-1 P
$ther sts:
None P 2ye:
2ye:
Eithei es 'P 0-# #-? ?-10 10-#? #?-?0 ?0 0-# 'P
euocytes (;
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
18/19
Urinalysis
U.2. Manual Urinalysis Microscopic Exam
omments:
-
8/9/2019 DownloadsPro68-01 Manual Urin Micro SOP
19/19
Your Lab NameAen*i3 @ - &rine ryst Proerties
#?8=##@@=.*oc P%e 999 of 1! Effective: Dte