Work Package 6: Biomolecular motors on nanostructures
Midterm-Review Meeting
Molecular Machines- Design and Nano-Scale Handling of Biological Antetypes and Artificial Mimics
Specific Targeted Research Project (STREP) in FP6-NMP-2002
Objectives
Partners
PM
Construct kinesin-motor based biological-inorganic hybrid devices for controlled nano-transport processes.
Construct kinesin-motor based biological-inorganic hybrid devices for controlled nano-transport processes.
ICG-GC TU/d-MB EPFL ETH
30 30 24 6
Midterm-Review Meeting
The molecular motors Work Package 6: Biomolecular motors on nanostructures
Cytoskeleton motors : Kinesin, Myosin...Cytoskeleton motors : Kinesin, Myosin...
Microtubules Kinesin
f-actin Myosin
How do they work ?Energy conversion
(M6-1) Structural changes vs. ATP hydrolysisHigh resolution techniques
How include the motors in nano-devices ?
(D6-1) Hybrid organic/inorganic systemsNano-handling
APD
DetectionDetection
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Traveling Wave Tracking Work Package 6: Biomolecular motors on nanostructures
Single 10 nm step
RMS noise ± 2 Å
Piezo: 10 nanometers steps
Time resolution ~ 2 µs
RMS noise ~ 2 Å
Drift ~ 1 nm/s
Midterm-Review Meeting
Traveling Wave Tracking Work Package 6: Biomolecular motors on nanostructures
- +Many motors ?
- +Single motor ?
T = 300 K
x = 7.4 pN s/nm
x = 0.3 pN/nm
Average speed = 500 nm/s
Backward steps ~ 10 %
Step time scale < 70 µs
Midterm-Review Meeting
Work Package 6: Biomolecular motors on nanostructures
Midterm-Review Meeting
Structures designed with a 3D approach for the TWT setup (collaboration with the Institut Curie)
Glass
3D approach
Some samples
Work Package 6: Biomolecular motors on nanostructures
In order to observe 3D movements, including rotations, the object of study has to be suspended
Soft Lithography approach
Midterm-Review Meeting
Work Package 6: Biomolecular motors on nanostructures
Nanostructures for kinesin-driven microtubule motility
Rectification, confinement and electrical docking
Time sequence of microtubule
docking
Midterm-Review Meeting
Rectification of kinesin-driven microtubule motility
SEM image of rectifying structure
Fluorescence image of microtubule motility in nanostructures
Rectifier geometries
92 % efficiency in rectification
Work Package 6: Biomolecular motors on nanostructures
Midterm-Review Meeting
Specific Targeted Research Project (STREP) in FP6-NMP-2002
Work Package 6: Biomolecular motors on nanostructures
Production Van den Heuvel, M. G. L.; Butcher, C. T.; Lemay, S. G.; Diez, S.; Dekker, C. Nano Lett. 2005, 5, 235-241.Van den Heuvel, M. G. L.; Butcher, C. T.; Smeets, R. M. M.; Diez, S.; Dekker, C. Nano Lett. 2005, 5, 1117-1122.L. Busoni, A Dornier, J-L Viovy, J. Prost, G. Cappello; Journal of Applied Physics 2005, 98 A. Dupont, C. Symonds, L. Busoni, J. Prost, G. Cappello; In preparation (2005)
M6-1 M6-2 and D6-1
Many different nanofabricated structures were made in TU-Delft New flexible optical technique, called Travelling Wave Tracking (ICG-GC). Microtubules injected in the structures (TU-Delft) and the activity has successfully been checkedFirst experiments on single molecular motor
Resources Post-Docs: Irene Dujovne, Clémentine Symonds, Lorenzo BusoniConferences and Meetings
additionalprojects
New motors: Myosin V and VI (collaboration with J. Spudich, Stanford)Gold nanoprobes (collaboration with R. Levy, University of Liverpool)DNA associated motors
Conclusion Objectives for the first part of the project have been achieved and we can begin measuring single molecular motors moving on the suspended microtubules.