Download - Water Soluble Vitamins
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Water Soluble Vitamins
Dr. Nasim
AP biochem
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Definition and Classification
Non-caloric organic nutrients Needed in very small amounts Facilitators – help body processes
proceed; digestion, absorption, metabolism, growth etc.
Some appear in food as precursors or provitamins
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Definition and Classification
2 classes, Table 7.1– Fat soluble:
– Water soluble:
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Water Soluble Vitamins: Characteristics
Essential Organic Structure Non-energy Producing Micronutrients Stability Bioavailability Toxicity
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Fat vs. Water Soluble Vitamins
Characteristics Water Soluble Fat Soluble
Absorption Directly to blood
Lymph via CM
Transport free Require carrier
Storage Circulate freely In cells with fat
Excretion In urine Stored with fat
Toxicity Possible w supplements
Likely w
supplements
Requirements Every 2-3 days Every week
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ThiaminStructure
–pyrimidine ring– thiazole ring–methyl bridge
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Thiamin : vitamin form
Thiamin pyrophosphate: coenzyme form
Pyrimidine ring Thiazole ring
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Chemical Characteristics
Very labile nutrientHeat
– stable in crystalline form– less stable in solution
Alkali - very unstable with heat– baking soda
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Chemical Characteristics
Sulfites - decomposes B-1High cooking/processing
losses– heat – leaching
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Absorption of B-1in duodenumactive transport (low thiamin
levels)– requires sodium and folic acid
passive transport (hi B-1 levels)
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Absorption of B-1phosphorylation to active
form inside cells (TPP)transported via portal bloodno significant storage,
excess to urine
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Biochemical Functions of B-1
Oxidative Decarboxyation Reactions
Pyruvate Dehydrogenase– Pyr+CoA+NAD --> AcCoA+CO2 +NADH
a-keto-glutarate dehydrogenase– aKG+CoA+NAD-->SuccCoA + CO2+NADH
important in CHO/energy metabolism
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Pyruvate + CoA + NAD+ ------> CO2 + acetyl-CoA + NADH + H+
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Biochemical Functions of B-1
Transketolation– HMP pathway
Peripheral Nerve FunctionTPP or TPPPnon-cofactor functionmechanism?
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Thiamin Deficiency
Beri-Berianorexia, fatigue, depressioneffects on
– cardiovascular system– nervous system
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Infantile Beri-Beri first 6 months breast milk deficient in B-1 mother w/o symptoms rapid onset cyanosis, tachycardia, labored
breathing heart failure and death
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Wet Beri Beri symptoms similar to congestive
heart failure Pitting edema - trunk, limbs, face labored breathing, tachycardia rapid deterioration fatal cirulatory collapse responds rapidly to B-1
supplements
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Dry Beri-Berino edemaprogressive wastingnumbing and
weakening of extremities
chronic infections
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Assessment of Thiamin Status
Urinary thiamin excretionBlood or serum thiamin
concentration[pyr + lac] in blooderythrocyte transketolase
activity– stimulation with B-1
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2000 RDA for Thiamin
Males
19-30yrs
Females
19-30yrs
RDA mg/d 1.2 1.1
EAR 1.0 0.9
NHANES III
Mean intake
1.78 1.45
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Friday’s Quiz Read:
– Riboflavin– Vitamin B-6– Biotin– Pantothenate
Know– Functions– Cofactor and vitamin forms– Deficiency and toxicity symptoms and
causes
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NiacinStructureNicotinic Acid = NiacinNicotinamide =
Niacinamide
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Cofactor Forms of Niacin
Nicotinamide Adenine Dinucleotide– NAD– nicotinamide-ribose-PP-ribose-adenine
Nicotinamide Adenine Dinucleotide Phosphate– NADP– nicotinamide-ribose-PP-(ribose-P)-adenine
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Nicotinic Acid (Plant form)
Nicotinamide (animal form)(reduced form)
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Nicotinamide Adenine Dinucleotide
nicotinamide
adenine
If Phosphate here ->NADP
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Chemical Characteristics of Niacin
relatively stable to– light– heat– oxidation– alkali
major losses due to leaching
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Digestion and Absorption of Dietary Niacin
Coenzyme form in food hydrolysis in small intestine to free
vitamin absorbed in duodenum nicotinic acid protein bound in corn
– requires alkali treatment (lime) to release niacin
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Metabolism of B-3
conversion of free vitamin to coenzyme in all cells
no storageexcesses metabolized in liver
to variety of chemicalsmetabolites excreted in urine
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Synthesis of B-3
from Tryptophan pathway requires B-6 (also B2) 60 mg of TRY required to make 1
mg B-3 corn is low in both B-3 and TRY
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Biochemical Functions of B-3
Oxidation-Reduction Reactions (NAD/NADH–Dehydrogenases
–Electron Transport System
–Involved in energy production
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Pyruvate + CoA + NAD+ ------> CO2 + acetyl-CoA + NADH + H+
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Biochemical Functions of B-3
Synthetic Pathways (NADPH)–FA synthesis
–Cholesterol synthesis
–NEAA synthesis
–Purine & Pyrimidine synthesis
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Deficiency of B-3 Pellegra Dermatitis
– scaly dermatitis, sun exposed
Dementia– confused, disoriented
Diarrhea– irritation/inflammation of mucous membranes
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Assessment of B-3 Status
Urinary excretion of niacin metabolites– N-methyl nicotinamide– 2-pyridone
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2000 RDA for NiacinNiacin Equivalents (NE)1 NE = 1 mg B-3 = 60 mg TRY
Males
19-30 yrs
Female
19-30 yrs
RDA (NE/d) 16 14
EAR (NE/d) 12 11
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Niacin Toxicity 1-3g/day for treatment of
hypercholesterolemia increases histamine release
– skin flushing– increase risk of peptic ulcers
liver injury time release forms greater risk of liver
injury
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Folic Acid / FolacinStructure
– pteridine ring - PABA - glutamate
Stability– very sensitive to heat – easily oxidized– leached
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Digestion & Absorption dietary form: polyglutamyl folate
– glutamate gamma linked Folate conjugase
– Zinc deficiency– alcoholism– drug interactions
folate absorbed as monoglutamate (free folate)
dietary supplement: free folate
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Folate Metabolism Intestinal Cells folate reduced to tetrahydrofolate
– folate reductase inhibited by methotrexate (chemotheraputic drug)
methylated to N5-methyl-THF– primary blood form
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Folate FunctionsSingle carbon metabolism
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Folate Functions Interconversion of serine and
glycine ser + THF <---> gly + 5,10-Me-THF Degradation of histidine his->->->formiminoglutamate(FIGLU) FIGLU+THF -> glu + 5-forminino-THF histidine load test
– Functional test for folate status
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Folate Functions Purine and Pyrimidine
Synthesis dUMP + 5,10-Me-THF -> dTMP + THF Methionine Synthesis homocysteine + 5-Me-THF -> MET +
THF MET as a methyl donor for choline
synthesis
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Folate Deficiency Megaloblastic Anemia decreased DNA synthesis failure of bone marrow cells to divide normal protein synthesis results in large immature RBC’s contrast with microcytic hypochromic
anemia
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Folate Deficiency Homocysteine
– Coronary Heart Disease risk factor ? genetic homocystinuria - premature
CHD hi [homocys] related to hi CHD risk lo [folate, B-12, B-6] related to hi CHD
risk lo intake of B-vit related to hi CHD risk
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Folate and Neural Tube Defects
Defects in formation of neural tube (brain & spinal cord)
First two months gestationAnencephaly
– absence of cerebral hemispheres
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Folate and Neural Tube Defects
Spina bifida– defective closure of vertebral column
– spinal cord protrusion from spinal column results in damage to spinal cord
– lower limb and hip paralysis
– rectal and bladder problems
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NTD PrevalenceUS:
– 4000 live births with NTDs/yr– 1/1000 pregnancies
World:– 400,000 live births with NTDs/yr
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NTDs and Folate NTDs associated with mothers with
low blood [folate] Estimated that 50% of NTDs
prevented with folate supplementation w/ 200 ug/d
DRI adults = 400 ug/d DRI prenancy = 600 ug/d typical US intake = 280-300 ug/d
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Folate and Grain Enrichment
Jan 1, 1998 140 ug/100g enriched grain results in additional 100 ug/d may reduce about 25% of NTDs limited because of masking of B-
12 deficiency
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Folate: 2000 DRI
Dietary Folate Equivalents (DFE)– 1 DFE =
1 ug food folate 0.6 ug fortified food folate taken with food 0.5 ug folate supplement on empty stomach
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Folate DRI (2000)Males
19-30 yr
Females
19-30 yr
RDA (ug/d) 400 400
EAR (ug/d) 320 320
UL (ug/d) 1000 1000NHANES III
Median intake (prior to fortification)
277 223
For women capable of becoming pregnant, it is recommended that they consume 400 ug of folate as supplements or fortified foods in addition to folate containing foods.
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Vitamin B-12Structure cobalamine methyl cobalamine
– transport and coenzyme form
adenosyl cobalamine– storage and
coenzyme form
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Dietary Sources Animal products
– including milk and eggs GI microorganisms Vegan sources
– N-fixing legumes– fortified grains– vitamin supplements
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Digestion & Absorption of B-12
Protein bound in foods released by acid and pepsin
– Elderly at risk R-protein gastric secretion binds with free B-12 protects B-12 from bacterial use ?
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Digestion & Absorption of B12
Intrinsic Factor gastric glycoprotein binds with B12 in small intestine
IF-B12 complex binds to B12receptor in ileum for absorption
B12 absorption requires functioning stomach, pancreas, and ileum
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Causes of B-12 Deficiency
Inadequate intake - rare DRI adults 2.4 ug/d Usual intake 7-30 ug/dMalabsorption of B-12 IF deficiency other GI tract problems
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Shilling Test for Malabsorption
Saturation of B12 by injection Oral administration of radiolabeled
B12
– free B12
– IF-B12
Measure urinary excretion of labeled B12
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Functions of B12Homocysteine to Methionine
– methionine synthetase requires 5-methyl THF deficiency of B12 results in “methyl-
trap” of folate– results in megaloblastic anemia– synergistic effect of B12 and folate
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Functions of B12Mutases methyl malonyl CoA mutase proprionyl-CoA ->->succinyl-CoA accumulation of methyl-malonate
may inhibit AcetylCoA carboxylase
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B-12 Deficiency Pernicious anemia megaloblastic anemia
– Methyl-folate trap – Delayed or failure of normal cell division due to
impaired DNA synthesis neuropathy
– defective myelination– progressive peripheral weakening– unresponsive to folate– upper limit to folate supplementation/enrichment
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Vitamin C - Ascorbic Acid
Structure Metabolism
– oxidation/reduction– dehydroascorbic acid– dehydroascorbate
reductase– glutathione (GSH)
glutamate-cysteine-glycine
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Functions of Vitamin C
Enhances absorption of iron
reduces iron to more absorbable ferrous form
chelates with ferrous ion to make it more soluble
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Functions of Vitamin C
Hydroxylation of proline and lysine
post-translational reaction of procollagen
hydroxylated collagen can be cross-linked to triple helix collagen
Scurvy - weak collagen
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Functions of Vitamin C
Hydroxylation Reactions Involves O2 and metal coenzyme
– (ferrous, cuprous) Carnitine synthesis Tyrosine synthesis & catabolism
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Functions of Vitamin C
Hydroxylation ReactionsSynthesis of Neurotransmitters
– Dopamine– Norepinephrine– Serotonin
Bile acid synthesis
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Functions of Vitamin C
Antioxidant ActivityReacts and removes active
oxygen speciesPro-oxidant ActivityReduces metals to their pro-
oxidant forms
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Scurvy Bleeding gums petechiae easy bruising impaired wound healing and
bone repair joint pain anemia
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RDA for Vitamin C
10 mg/day prevents scurvyhistoric RDA’s 45-70 mg
(60mg in 1989), 75mg in 2000prevention of scurvy vs
antioxidant effect with supplements?
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Toxicity of Vitamin C UL adults: 2000mg/d Osmotic diarrhea Oxalate kidney stones Decreases uric acid reabsorption
resulting in increased risk of gout Affects diagnostic tests in feces and
gout– fecal blood– urinary glucose
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Variety is the Key
Vitamins are
derived from
a variety of
foods.