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1395
Dual-Channel Fluorescence Mode
Video-Rate Confocal Laser Scanning Microscopy KitsConfocal Reflectance Imaging Kit:Thorlabs’ Video-Rate Confocal Imaging System can be configuredfor reflection and fluorescence imaging modes. Confocal reflectancemicroscopy is ideal for surface imaging of highly reflective, opaquematerials such as microelectronics as well as for metrology studies(Fig. 2). The VCMK-R includes all components necessary to addreflectance confocal imaging to pre-existing microscopes.Configuration of these components are outlined in Fig. 1. Pleasecall or refer to our website for details or custom options.
S1FC635Benchtop Laser Source
Resonant Scanner
Flat Mirror
Scan Lens
Intermediate Image Plane
Tube Lens
Microscope
Infinity-correctedObjective
VCM-RConfocal Base System
GalvoScanner
ConfocalControl Unit
VCM-ZS500Z Stage
Fiber Collimator
Signal / Control Route
Beam Splitter
Optical Fiber
PDA8A(/M)Photo Diode
VCMK-R Components
ITEM# Description Page
VCM-R Confocal Base System, Reflectance 1397
PDA8A Photodiode Detector 1288
VCM-ZS500 Z Translation Stage 1400
Laser Source
S3FC473 Benchtop Laser Sources 1058
Confocal Reflectance ModeFigure 2. Confocalreflectance image of amemory chip with XZand YZ cross-sectionalimages taken with a60X objective.
75 µm55 µm
+200
-20
Multimode FiberSingle Mode FiberPCU2B
PMT Module
VCM-BC2Beam Combiner
Single Mode Fiber
VCM-PH5Pinhole Selector
Tube LensFiber Collimator
Emission Filters
Dichroic Beamsplitter
Signal / Control Route
Optical Fiber
Resonant Scanner
Flat Mirror
Scan Lens
Intermediate Image Plane
Microscope
Infinity-correctedObjective
VCM-ZS500Z Stage
VCM-FConfocal Base System
GalvoScanner
ConfocalControl Unit
Figure 4. Dual-Channel Fluorescence Configuration
VCMK-F Components
ITEM# Description Page
VCM-F Confocal Base Systems, Fluorescence 1397
PCU2B High-Sensitivity Dual PMT Unit 1398
VCM-BC2 Two-Channel Beam Combiner 1399
VCM-PH5 5-Position Pinhole Selector 1399
VCM-ZS500 Z-Translation Stage 1400
Laser Source
MCLS1 Four-Channel Laser Source(Includes 406 and 473 nm Lasers) 1402
Dual-Channel Confocal Fluorescence Imaging Kit:Many fluorescence applications use multiple dyes to enhancecontrast and differentiate specific cell structures or quantifymetabolic processes (Fig. 3). Thorlabs has developed a Dual-Channel Confocal Fluorescence Imaging Kit (VCMK-F) for easyassembly of a confocal fluorescence imaging system that providestwo wavelength excitation (406 nm and 473 nm) and detection(405 nm and 561 nm). Figure 4 demonstrates configuration of theVCMK-F components for dual-channel fluorescence imaging.Please call or refer to our website for details and custom options.
Figure 1. Confocal Reflectance Imaging Configuration Figure 3. Dual-channelfluorescence image ofF-actin and nucleidistribution in bovinepulmonary arteryendothelial (BPAE)cells obtained withThorlabs’ ConfocalSystem. The image wasrecorded using simultaneous scanning of both 403 nm and 471 nm laser sources.
ITEM# $ £ € RMB DESCRIPTIONVCMK-R $ 35,000.00 £ 24,263.00 € 31.074,00 ¥ 295,541.00 Confocal Reflectance Imaging KitVCMK-F $ 65,000.00 £ 45,060.00 € 57.708,00 ¥ 548,862.00 Dual-Channel Confocal Fluorescence Imaging Kit
