1 2 3
Barriers to access synbio
The access is hampered by the required equipment, knowledge
and economic costs.
Human Practices
Hardware
Printeria combines multiple technologies to automatically produce genetic circuits.
1 Entry zone
BioInk (Bioparts)The BioInk is positioned in a carousel-based system, so a needle produces the fall of the assembly reagents.
Components• Carousel system• Peristaltic pump
• DNA basic pieces
• BsaI endonuclease, T4 DNA ligase
• Enzyme Buffer + BSA
• Destination vector
2 Reaction zone
Experimental and simulation results
ComponentsFor drop movement• Printed Circuit Board (PCB) • Surface: PTFE foil and
hydrophobic gelFor changing temperatures• Power resistor (37ºC zone)• Peltier effect (16ºC zone)
3 Output system
Here, the optimal conditions for growth and measuring Optical Density (OD) and Fluorescence (F) are set.
Components• Shaker• 650nm Laser• 480nm led• Light sensor
Extracellular AHL
Intercellular AHL (A) Lux R (R)
Lux R·AHLR.A
(Lux R·AHL) dimer(R.A) 2
gGFP
GFP
mGFP
TRANSCRIPTIONTRANSLATION +
LuxI promoter Plux
mR
An intuitive and user-adapted interface allows the user to design and print genetic circuits by selecting the basic DNA pieces.
Before printing a genetic circuit, it can be simulated with our Simulation Tool, or it can be directly printed.
These pieces are part of our Golden Gate standardized Part Collection, and they are fully characterized to provide the user all the information about the designed genetic circuit.
An extensive repository of pre-designed recipes for genetic circuits can be printed too.
Software
Printeria uses the Level 1 Golden Gate assembly method, so the multipartite directional assembly of a composite part (transcriptional unit) is carried out by a high robust one-step reaction in a drop.
The destination vector is introduced in its linearized form to avoid the screening of non-recombinant transformed plasmids.
With our ODE modeland the experimental data we have estimated model parameters and characterized all the parts in our Printeria part collection.
Applying different voltages to the pads in the PCB surface, local hydrophobicity changes. This makes the droplets move between the hot and cold zones.
For each Printeria recipe we take information from our database and combine it to get a ODE model of the new part. The user can predict the behavior of the design before printing.
Level 1 Golden Gate
Droplets movement
525
500
475
450
425
4000 50 100 150 200 250 300
Lux R
1800
1600
1400
1200
1000
8000 50 100 150 200 250 300
Protein of Interest
15k
12.5k
10k
7.5k
5k
2.5k
00 50 100 150 200 250 300
Number of cells
Simulation tool
Thermal image of the PCB showing the temperatures of the different zones
Calibration system
Parts characterization
Printeria Experiments
Printeria’s goal is to democratize SynBio and make it accesible to everyone: BioArtist, Research Labs, Teachers, and Students.
Integrated HumanFeedback from students (with the Kano Model) and renowned scientists and artists was taken into account in order to improve our device’s functionalities.
Social EngagementWe understand BioArt as the point where art and science meet, with art being the cornerstone of dissemination.
Educational approach to SynBio.
Yturralde and his Impossible Figure
María Peñil andMehmet
Carol from Printeria’s Team with Francis Mojica
GFPmut3b / GFPmut3b + LVA tag
Changing RBS and promoter strength
TU
BBa_K2656105
BBa_K2656106
BBa_K2656107
BBa_K2656117
BBa_K2656108
sfGFP
Changing RBS strength
TU
BBa_K2656101
BBa_K2656100
BBa_K2656102
BBa_K2656103
BBa_K2656104
mRFP1 / mRFP1 + LVA tag
Changing RBS strength
TU
BBa_K2656109
BBa_K2656118
BBa_K2656119
BBa_K2656110
Blue chromoprotein
Changing RBS strength
TU
BBa_K2656113
BBa_K2656120
YFP / YFP + LVA tag
TU
BBa_K2656112
BBa_K2656111
The calibration curve shows experimental data measured by Printeria’s sensors vs. the flourescein molecules number per cell (MEFL
GFP/cell) from our
Interlab Study.
Calibrated Optical Density curve made by Printeria’s OD sensor.
MEF
L GFP
/cel
l
Prot
ein
(MEF
L GFP
/cel
l)
Valencia UPV iGEM 2018 Team