![Page 1: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/1.jpg)
Transcription
Tuesday, November 14, 17
![Page 2: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/2.jpg)
OverviewDNA
RNA
Protein
Tuesday, November 14, 17
![Page 3: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/3.jpg)
OverviewDNA
RNA
Protein
Transcription
Translation
Tuesday, November 14, 17
![Page 4: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/4.jpg)
Don’t Write This Down . . .
If the cell wants to make a specific protein it will take a ‘photocopy’ of the section of DNA that carries the information for specific protein
This photocopy is known a RNA
Tuesday, November 14, 17
![Page 5: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/5.jpg)
DNA RNA
deoxyribose, phosphate, nucleotide base
ribose, phosphate, nucteotide base
G, C, A, T G, C, A, U (Uracil)
double stranded single stranded
stable unstable
in the nucleus everywhere
Tuesday, November 14, 17
![Page 6: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/6.jpg)
Tuesday, November 14, 17
![Page 7: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/7.jpg)
Types of RNA
mRNA: Messenger RNA (transcription)tRNA: Transfer RNA (translation)rRNA: Ribosomal RNA (translation)
Tuesday, November 14, 17
![Page 8: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/8.jpg)
Stages of Transcription
1) Initiation2) Elongation3) Termination
Tuesday, November 14, 17
![Page 9: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/9.jpg)
Initiation
OverviewRNA polymerase binds to a promoter region
Tuesday, November 14, 17
![Page 10: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/10.jpg)
Initiation
A promoter is a DNA sequence that is high in adenine and thymine (TATA box)It is adjacent to the gene to be transcribed
Tuesday, November 14, 17
![Page 11: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/11.jpg)
Initiation
A promoter acts as a recognition/binding site for the TATA binding protein.
This protein is recognized by RNA polymerase II which docks to DNA
Tuesday, November 14, 17
![Page 12: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/12.jpg)
Initiation
RNA polymerase unwinds the DNA and the template strand is exposed.
Only on strand of the DNA is transcribedThe promoter is NOT transcribed
Tuesday, November 14, 17
![Page 13: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/13.jpg)
Tuesday, November 14, 17
![Page 14: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/14.jpg)
Elongation
RNA polymerase binds with the promoter and builds in a 5’ → 3’ direction
Tuesday, November 14, 17
![Page 15: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/15.jpg)
Elongation
No Okasaki fragments are created because only one strand is transcribed (no lagging strand)
Tuesday, November 14, 17
![Page 16: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/16.jpg)
Elongation
RNA polymerase works faster than DNA polymerase because there is no proof reading.
Tuesday, November 14, 17
![Page 17: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/17.jpg)
Elongation
Uracil complements adenine
Tuesday, November 14, 17
![Page 18: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/18.jpg)
Tuesday, November 14, 17
![Page 19: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/19.jpg)
Termination
A stop codon signals RNA polymerase to end transcription
UAA, UAG, UGA
Tuesday, November 14, 17
![Page 20: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/20.jpg)
Termination
mRNA and RNA polymerase are releasedDNA that was being transcribed rewinds into double-helical form
Tuesday, November 14, 17
![Page 21: Transcription - Ms. kropac€¦ · RNA Protein Transcription Translation Tuesday, November 14, 17. Don’t Write This Down . . . If the cell wants to make a specific protein it will](https://reader033.vdocuments.us/reader033/viewer/2022050113/5f4aa01b7d711a45e8783f41/html5/thumbnails/21.jpg)
Tuesday, November 14, 17