Download - The investigation of BBB score of animal behavior in rat based on high-speed camera analysis
The investigation of BBB score of animal behavior in rat based on high-speed camera analysis
• Presenter : Bo-Ying Syu• Adviser : Dr. Pei-Jarn Chen• Data : 2013/6/5
Outline
Introduction Papers Review Material and Methods Future works References
Spinal Cord Injury Incidence of 40 new cases per million people
throughout the world each year Mean age : 28.6 years old Sensory and autonomic dysfunctions, the degree of which is characteristic for the severity of the SCI
Introduction ( 1 / 6 )
Experimental studies of pharmacology, neuropathology and pathophysiology commonly measure the motor function of rats.
The behavioural performance can be used as an index for neural disease process or therapy efficiency.
Introduction ( 2 / 6 )
Use Aneurysm-clip with spinal cord damage Destruction of parts T7、 T8、 T9
Thoracic Vertebrae
Introduction ( 3 / 6 )
Measurement methods
Locomotor tests Sensory tests Sensory motor testsMotor tests
Primary open-field tests BBB test Automated walkway test Footprint analysis test
Inclined plane Limb hanging test Limb grip strength test
Cold sensitivity-based tests Withdrawal reflexes Rope walk testing Narrow beam test Foot slip test
Primary open-field tests BBB test Automated walkway test Footprint analysis test
Pictures form " Methods for behavioral testing of spinal cord injured rats "Pictures form " An inclined plane system with microcontroller to determine limb motor function of laboratory animals"Pictures form " Methods for behavioral testing of spinal cord injured rats "
Introduction ( 5 / 6 )
Introduction ( 5 / 6 ) BBB test
Basso, Beatie and Bresnahan Test Two or three researchers as a group and record score, then average these score
BBB Score Divided into 0-21, degree is to be used to evaluate
the spinal cord injury.
Introduction ( 6 / 6 ) BBB Score
Scores from 0 to 7( Indicate the return of isolated movements in the hip 、
knee and ankle joints )Scores from 8 to 13
( Indicate the return of paw placement and coordinated movements with the forelimbs )
Scores from 14 to 21 ( Show the return of toe clearance during stepping, predominant paw position , trunk stability and tail position )
PAW PRINT GAIT ANALYSIS IN RATS WITH SPINAL CORD INJURY
MAY, 2007
MALU TANSEY ,KEITH TANSEY ,SHUXIN LI
Papers Review ( 1 / 2 )
Papers Review ( 1 / 2 )
Surgical procedure and contusion: Long-Evans rats (n=10) Destruction of parts : T9 (182 to 227
kdyn) Postoperative : Baytril
Material and Methods
High-speed video camera (fps=80) On the treadmill DigiGait analysis software BBB score
Papers Review ( 1 / 2 )
Material and Methods
Two to six weeks postoperatively 5 cm/s every thirty seconds Five consecutive strides
Papers Review ( 1 / 2 )
Material and Methods
Papers Review ( 1 / 2 )
Parameters were assessed: Stride length Stance width Stance-to-swing ratio
Material and Methods
Papers Review ( 1 / 2 )
Results
•Stride length increased 1.2-fold from the second to third week and then remained relatively constant at each time point. (stride/s at 25 cm/s)
Papers Review ( 2 / 2 )
A semi-automated software tool to study treadmill locomotion in the rat From
experiment videos to statistical gait analysis
May 2010
P. Gravela, , M. Tremblayb, H. Leblondb, S. ∗Rossignolb, J.A. de Guisec
Papers Review ( 2 / 2 )
MethodsAdult wistar rats (n = 6)High-speed area scan camera (fps=120, DS-41-300K0262 ,DALSA Inc ).C arm X-ray system (100 kVp/16 mAs~ 40 kVp/0.5
mAs)On the treadmill8~20 complete cycles
Papers Review ( 2 / 2 )
Methods
27 cm×27cm calibration grid
Image calibrationimage interpolation
Papers Review ( 2 / 2 )
Methods
From top to bottom; the iliac crest, the femoral head, the knee joint, the ankle joint, the metatarsophalangeal joint (MTP), and the distal phalange of the second toe.
Image contrast enhancement stationary wavelet transform (order 5). Using cubic smoothing spline
iliac crestfemoral headknee jointankle jointMTPthe second toe
Hip angles knee angles
heel angles MTP angles
Papers Review ( 2 / 2 )
ResultsThe data were acquired manually without the GUI (1 h 30 min)
semi-automatically with the GUI (12 min)
Use a High-speed camera to analyze animal behavior.
Combination of the BBB scores and gait parameters to measure the rat activity behavior.
Purpose
High-speed cameras (fps=120, scA640-120gc, BASLER Inc)
Streampix 5 (Norpix Inc.) Windows XP、 C++ builder、 Opencv1.0
Material and Methods
Material and Methods
Side scan (Marker)
Material and Methods
YCbCr
Cb色板
Morphology
A
B C
a
b
c
x
Side scan (Marker)
Material and Methods
Side scan (Centroid)Morphology
(Gray)(Binarization)
(Erode&Dilate)
Material and Methods
Bottom scan(Centroid)
Material and Methods
(Gray)
(Binarization) (Erode&Dilate)
Bottom scan (Limbs)
(ROI)
bgrf IIII 11.059.07.0
Material and Methods
Results•Angle Analysis
Future works
Improve the bottom scan. Brain injury Shoot the two sides. Gait analysis and centroid combination to
find correlations. BBB score more correct.
References Methods for behavioral testing of spinal cord injured rats , Jirˇı´ Sˇ edy´ a,b, Lucia Urdzı´kova´ a,b, Pavla
Jendelova´ a,b, Eva Sykova´ a,b , Received 15 March 2007; received in revised form 9 August 2007; accepted 3 October 2007
Automatic analysis of altered gait inarylsulphatase A-deficient mice in the open field , Toon Leroy, Stijn Stroobants , Jean-Marie Aerts , Rudi D’Hooge, and Daniel Berckmans , Behavior Research Methods , 2009, 41 (3), 787-794
Functional evaluation of peripheral nerve regeneration in the rat : walking track analysis , Artur S.P. Varejfio a,* Marcel F. Meek b Antdnio J.A. Ferreira °, JoS.o A.B. Patricio d Antdnio M.S. Cabrita e , Journal of Neuroscience Methods 108 (2001) 1-9
An inclined plane system with microcontroller to determine limb motor function of laboratory animals, Ming-Wen Chang, Ming-Shing Young, and Mao-Tsun Lin, Journal of Neuroscience Methods., 168: 186–194, 2008.
Robotic Assessment of Locomotor Recovery in Spinal Contused Rats , J.A.Nessler,D.J.Reinkensmeyer, K.Sharp,E.Kwak1,K.Minakata,R.D.DeLeon,26th Annual International Conference of the IEEE EMBS
Robot Applied Stance Loading Increases Hindlimb Muscle Mass and Stepping Kinetics in a Rat Model of Spinal Cord Injury, Jeff A.Nessler, Moustafa Moustafa-Bayoumi, Dalziel Soto, Jessica E. Duhon, Ryan Schmitt, 33rd Annual International Conference of the IEEE EMBS