Transcript
![Page 1: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/1.jpg)
The double-reciprocal (Lineweaver-Burk)
plot allows easy calculation of Km and Vmax
![Page 2: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/2.jpg)
Many factors influence the activity of an
enzyme
• pH
• Temperature
• Concentration of molecules that bind to enzyme
– Substrate
– Reversible inhibitors
– Irreversible inhibitors (inactivators)
– Allosteric effectors
• Covalent modification
• Enzyme concentration
![Page 3: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/3.jpg)
Enzymes show maximum activity at their pH
optimum
![Page 4: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/4.jpg)
Enzymes can be inhibited by substrate
analogs
![Page 5: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/5.jpg)
Enzymes are greatly inhibited by transition-
state analogs
Adenosine deaminase
Km = 3 x 10-5 Product inhibition:
KI = 3 x 10-4
TS analog:
KI = 1.5 x 10-13
![Page 6: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/6.jpg)
![Page 7: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/7.jpg)
![Page 8: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/8.jpg)
![Page 9: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/9.jpg)
![Page 10: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/10.jpg)
α is the factor by which [S] must be increased
to overcome the presence of inhibitor
![Page 11: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/11.jpg)
![Page 12: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/12.jpg)
![Page 13: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/13.jpg)
![Page 14: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/14.jpg)
![Page 15: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/15.jpg)
![Page 16: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/16.jpg)
![Page 17: The double-reciprocal (Lineweaver-Burk) plot allows easy ...rebecca/153A/W11/Lectures/153A... · The double-reciprocal (Lineweaver-Burk) plot allows easy calculation of K mand V max](https://reader030.vdocuments.us/reader030/viewer/2022040523/5e8708ce2f046b6d9a0f3a7b/html5/thumbnails/17.jpg)