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The Controversy About the H5N1 Transmissibility Experiments
http://www.nature.com/news/work‐resumes‐on‐lethal‐flu‐strains‐1.12266
3 M AY 2 0 1 2 | VO L 4 8 5 | N AT U R E | 1 3
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Learning Objectives
• Participants will be able to:– Describe the details of the experiments in question.
– Explain why the research is considered as “dual use”
– Examine the role of scientists in contemporary society
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H5N1 viruses Highly pathogenic avian influenza (H5N1) virus –is avirus that occurs mainly in birds, is highlycontagious among birds, and can be deadly tothem, especially domestic ones. Few sporadichuman infections with this virus have occurred andcaused serious illness and death.
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Summary of the issuesStudies by Ron Fouchier at Erasmus Medical Center inRotterdam, Netherland and Yoshihiro Kawaoka at theUniversity of Wisconsin, USA, aimed to answer a question“whether H5 influenza viruses can achieve sustainableaerosol transmission in ferrets?”
http://www.inferno‐guide.net/chapitre102/
Ron FouchierYoshihiro Kawaoka
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• They did repeat passage and replication of H5N1 inferrets, a well established animal model for thestudy of influenza transmission.
• Ferrets are also more likely than humans to havedisseminated, multiorgan influenza diseaseincluding neurologic sequelae (consequences)resulting from virus replication in the brain (ZitzowLA, et al, 2002).
• Their results showed that mutant strains of H5N1can achieve sustainable aerosol transmission inferrets
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3 M AY 2 0 1 2 | VO L 4 8 5 | N AT U R E | 1 3
The Kawaoka Experiments
Random mutation produced about 2.1 million strains –one could now bind the human/ferret receptor
That H5N1 was reassorted with the 2009 H1N1 virus (mix two viruses) and injected into ferrets – a third mutation
led to airborne transmission
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3 M AY 2 0 1 2 | VO L 4 8 5 | N AT U R E | 1 3
A fourth mutation that stabilized virus entry caused effective airborne transmission between ferrets
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Activity 1 ‐15 minutesResearch the five steps in influenza replication
• Group 1: virus attachment• Group 2: cell entry• Group 3: nuclear entry and transcription• Group 4: transcription and replication• Group 5: assembly and budding
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Linda Stannard http://www.rapidreferenceinfluenza.com/chapter/B978‐0‐7234‐3433‐7.50009‐8/aim/virus‐replication
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Authors(Group 1)
Jigsaw model (4 groups)
Regulatory agency(Group 2)
WHO(Group 3)
Public(Group 4)
Media(Group 5)
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Activity 2 – Role play and jigsaw
Group 1: The authors, who support and justify the research approachGroup 2: A government regulatory agency in Indonesia concerned with dissemination of information
Group 3 : The WHO, who has to balance open publication with public safety
Group 4: The public, who are frightened and skepticalGroup 5: The media, who overreact to the issue
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Time line of H5N1 dual use events
MALTAmeetingSept 2011
OCTOBERmanuscripts arrive at NSABB*
NSABB advises Redaction/limitedAccess Nov 21
Jan 2060 dayvoluntary moratorium
WHO opposesredactionFeb 17
Mar 30NSABBReversesdecision
May 2 Kawaokapaper published
Jul 31 Flu meeting NYCNIH (Fauci) advises that moratorium be continued
2011‐2012
Jun 21 Fouchierpaper published
Dec 17‐18NIH meetingon flu researchregulations
*National Science Advisory Board on Biosecurity
Jan 23 2013 controversy ended by the resumption of research
Oct 17, 2014
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As a group, list the elements that arise in the H5N1 case
Activity 3 – concept mapping
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An example of a concept map
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As a group, now link these elements together in a concept map
Activity 3 – concept mapping
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Conclusions
• Scientists should understand that their research may be misused or misapplied to harm society.
• Scientists must take into consideration the essential procedures to minimize and prevent the misuse of their research.