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The Alfin-like PHD Zinc Finger Transcription Factor
Family in Arabidopsis Thaliana
Melissa Borkenhagen8 May 2006
HC70ALDR. Bob Goldberg
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Gene AT5G20510
5’ UTR
EXONINTRON
T-DNA
LBb1FW
Chromosome 5
T-DNA INSERT ACCORDING TO SALK
3’ UTR
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Gene AT3G42790
3’ UTREXONINTRON
T-DNA
LBb1
FW
Chromosome 3
T-DNA INSERT ACCORDING TO SALK
5’ UTR
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In which plant organs are the genes active?
Gene AT5G20510
Gene AT3G42790
Consistent with GeneChip Data, both genes appear to be active in these plant organs.
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Gene AT5G20510 Plant Genotypes
Strange double bandsWhat are the genotypes?
Only one T-DNA Insert
Plant 1 is the only Homozygous Mutant Plant
Separation of primers
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Gene AT3G42790 Plant Genotypes
Plants 1 and 4 are Homozygous Mutants
Plant 5: Heterozygous?
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Are there any observable phenotypic differences between
wild type and mutant plants?
No significant differences observed between wild type and mutant plants.
Wild Type
Mutant
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Is continued work on this family of transcription factors
important?Research has shown that members of the
alfin-like PHD zinc finger transcription factor family may be influential in plant
development. Some appear to be particularly influential during seed
development, with a possible role in the differential seedling development in darkness and light. Thus, continued work on these proteins is important!