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______________________________________1. Name 2.Type3.Aim 4.Principal 5.Reagent 6. steps 7.Result 8. conclusion 9. MLI in spectroscope
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Blood : 6 tests
3 preliminary : • kastle mayer pink(20 gm Koh __20gm zinc_ 2gm phenolphthalein _100 ml water )
• benzidine _ (benzidine in glacial acetic acid )blue • guciacum (guciacum resin in ethanol ) green Peroxidase enzyme oxidation of reagent in O2 source colour3 confirmatory :• spectroscope (blood absorb light and give bands )_ distilled water1:102 bands (carboxy or oxy ) ___1 band (reduced Hb __( 4bands metHb )_ 2( one thick )hemachromagen )))))DON’T FORGET ( MLI) • Teichmann s __ )hematine )_anhydrous glacial acetic acid_ rhombic
crystal • takayama (haemochromagen ) __ 3v( NAOH _ Pyridine _ glucose )
7v (water ) needle shape crystales
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Semen : Florence choline 1.5gm K iodide __2.5gm iodine __30ml water
Rhombic shape small then enlarged disappear choline per iodide
Barberios spermin alcoholic solution of picric acid needle shape crystal
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Reinsch test
1. Name : reinsch2. Type : preliminary
3. Aim : diagnosis heavy metals
4. Principal : heavy metals deposit on copper foil in presence of HCL
5. Reagent : 10% HCL+ copper foil 6. steps : contamination _ colour changes __ sublimation _
7. Result :…………………..8. advantages :easy _cheap _quick _ indicate small amount
9. Disadvantages : qualitative not quantitative
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Copper foil
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Hair and fibers
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medulla
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Silk
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Cotton
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wool
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