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Synthetic Biology - Progess in Merging Nanoparticles and Proteins
Kie Moon SungDavid MosleyJoseph JacobsonCenter for Bits and Atoms, MIT
Beau PeeleShuguang ZhangBiomedical Engineering Dept, MIT
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Nanoparticle Building Blocks
COOH
NH2
COOH
NH2
COOH
NH2
COOH
NH2
Amino acid-nanoparticles Natural amino acids
COOH
NH2
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Synthesis of Lys-Monofunctionalized Gold Nanocrystals
aReagents and conditions: (a) H2NNH2·H2O/DMF (2% v/v), DIPEA/DMF (10% v/v). (b)
HS(CH2)10CO2H (excess), THF. (c) DIC (excess), DMF/THF (10% v/v) rt, 24h. (d) MeNH2, DIC
(excess), DMF/THF, rt, 12h. (e) 60% TFA, 2.5% TIS, 2.5% H2O, 35% DMF, 24h.
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Identification of Monofunctionalized Nanocrystals
Nanocrystal Dimers
10 nm
At least 60% of Nanocrystals are monofunctionalized.
J.Am.Chem. Soc., 126 (2004), p. 5064
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Programmable Assembly of Nanocrystal-Peptides
Fmoc SPPS Protocols
peptide1 peptide2 peptide3
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New Structures - New peptide-folding rules governed by nanoparticles.
New Functions – Electronic control of specific protein or enzyme
functions, electronic feedback aka new biosensor techniques.
New materials – Both bioinorganics and a new class of nanoparticle
polymers.
Ultimate Goals
All aided by the programmable assembly nature of this building block based technology. This concept will allow us to access completely new motifs, and readily explore the phase space.