Synthetic Biology Crash CourseDAY TWO

Day Two Schedule1:00-1:10: Review Restriction Enzyme Digest1:10-1:30: Restriction Digest Activity 1:30-1:40: Review Gel Electrophoresis1:40-2:10: Gel Electrophoresis2:10-2:20: Review Transformation2:20-2:50: Transform and Plate Bacteria2:50-3:00: Visualize Gel

Review: Restriction Digest 1:00-1:10

Restriction Enzyme Digest

Restriction Digest Activity1:10-1:30

Review: Gel Electrophoresis1:30-1:40

Gel Electrophoresis

Gel Electrophoresis1:40-2:10

Review: Transformation2:10-2:20

Transformation Reaction

Transformation and Plating2:20-2:50

Gel Visualization2:50-3:00

HomeworkRead the following articles:

Do-It-Yourself Biology? Messing Around

with DNA Increasingly a Garage-Band

VentureYour Vanilla Ice Cream Is About to Get Weirder

Biohackers Are Kickstarting Some Unregulated Experiments