General Microbiology Lab
Page | 1 BIE 2127, Fall 2019
Syllabus
BIE 2101 Microbiology - BIE 2127 General Microbiology Laboratory
Term: Fall, 2019
Instructor: Dr. Erdal Eroglu
Department: Department of Bioengineering, Manisa Celal Bayar University
Office: C Block, Room#219 Office Phone: (0236) 201 2455
E-mail: [email protected] Web page: http://erdaleroglu.cbu.edu.tr
Credit: This is a 3 credits (2 hours theoretical and 2 hours laboratory) class.
Lab time: Every Wednesday from 13.15 PM to 14.55
Room#: Theoretical: C 209, Laboratory: C-Z12
Required textbook for theoretical course: Brock Biology of Microorganisms, by MT
Madigan, JM Martinko, 14th Edition (or 13th Edition), 2014.
Required textbook for lab course: Microbiology: A Laboratory Manual, by J.
Cappuccino and N. Sherman, 10th
Edition, Pearson Education 2014
Course Description:
This module is a major requisite for the students of bioengineering and it is presented in
lectures. Its contents focus on basic microbiological techniques involved in studying the
general characteristics of microorganisms and their growth requirement.
Course Objective:
Learning how to deal with different microorganisms in laboratory and studying the
general characteristics of microorganisms.
Lab Grading Policy
Short reports will be submitted at the end of the each lab. class. They will be worth 20%
of final grade.
Extra Credit:
Note: THERE ARE NO EXTRA CREDIT ASSIGNMENTS! In an effort to treat all
students in a fair and equitable manner, I do not allow extra credit work. Also, please do
not ask for extra point(s) at the end of the semester.
General Microbiology Lab
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Make-up Policy:
No make-ups will be given!!!! Students are allowed to drop one 100 point unit exam.
Dates of exams may change, so be sure to keep up with updated information.
Announcements and contact:
All course announcements, assignments and grades will be posted online using my
personal web page (http://erdaleroglu.cbu.edu.tr). You can also find the syllabus on the
tab “Classes” under “Categories” on the web page. Updates to this syllabus will be
posted; please check periodically.
Also, you can reach me by email ([email protected]) and the lab assisstants
anytime to ask your question or to discuss any topic/problem that you have.
Office hours:
Regularly scheduled office hours will be posted on my office door. These are times when
I will definitely be in my office, but I am happy to help at other times if my schedule
permits. Anytime I am in my office, please feel free to stop by. If you want to be sure of
my availability outside regular office hours, you can schedule an appointment.
Lab assistants are also available for Thursday at 4:00 PM. You can ask your questions
and observe the experiment results with them for the next day from the lab. Assistants
will be happy to answer your questions as possible as their programs allow.
Lecture Policy:
Students are expected to attend all lectures. Lectures are a necessary supplement to the
textbook. In the event of a missed lecture, the student is responsible for the lecture
material, any assignments which were given announcements or any other information that
was provided in class.
Lateness Policy:
This class is scheduled from 10.50 AM to 12.30 PM on every Tuesday. I will start
lecturing at 10.51 AM. You are not required to come to class, unless there is an exam.
Consistent lateness will result in loss of participation points and missed material. It is also
extremely disrupting to me and to your classmates. If you do come in the first 15 minutes
after lecture has started, please wait outside until I let you enter the classroom. Please sit
down as quietly and quickly as possible. If you are going to be more than 15 minutes late,
please do not come to class, wait until break time. The starting time for lab is 13:15 and
the same rules will be applied to this course.
Attendance at Exams:
Students are required to attend class on exam days. Make-up exams will not be given
General Microbiology Lab
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without a valid excuse. Validity of the excuse will be up to the discretion of the
instructor. Be forewarned; you will need to have a very good reason for missing an exam!
I am not trying to be harsh. I am only trying to treat all students in a fair and equitable
manner. If the student is aware of some responsibility which will interfere with an exam
date, it must be discussed with the instructor in advance. If an exam is missed without
advance notice due to illness or emergency, a valid written excuse will be required from
the doctor/school nurse in the case of illness in the case of a family emergency. If a
student does not have a valid excuse for missing an exam, the student will receive a zero
for the missed exam.
Classroom Protocol:
Appropriate classroom behavior is defined and guided by complete protection for the
rights of all students and faculty to a courteous, respectful classroom environment. That
environment is free from distractions such as late arrivals, early departures, inappropriate
conversations and any other behaviors that might disrupt instruction and/or compromise
students’ access to the education. Inappropriate classroom protocol may lead to removal
from the classroom and a lowering of the lecture grade. If continuous, it can lead to
forced withdrawal from the class.
Disabilities:
Students with documented disabilities who may need academic accommodations should
discuss these needs with their professor during the first two weeks of class.
Note to students:
Because of the wealth of info, some memorization will be needed to acquire a solid
background in microbiology, but we will also need to learn to apply that assimilated
knowledge. The chapter problems and problem handouts are designed to aid in that
aspect. Try to complete these problems on your own before resorting to the posted
solutions or solutions manual. I am not only interested in the material directly presented,
but also that you develop the ability to apply concepts to situations not previously
encountered. Do not treat this subject matter as a mere storehouse of facts. I do expect
you to predict the outcome of a particular situation from the background you will obtain
in the course.
Exams are intended to assess mastery of the subject not just familiarity with it. Answers
to exam questions need to be reasonable and use terminology appropriately. Do not do
them in your head and expect to complete them on paper when taking the exam.
Remember I need to grade what is on the paper; I cannot gauge what is in your head. If
you are not comfortable with the material, you are not ready for the exam. Please be
honest with yourself – seek help if you are not comfortable with the material. I will do all
I can to help you, but I can’t do it for you. It requires a serious effort on your part. I do
not gain any satisfaction from giving a student a low grade. However, I do need to
assess what you have learned and assign an appropriate grade based on that.
General Microbiology Lab
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Cheating and Scholastic Dishonesty:
All students are expected to be familiar with Cheating Policy & Procedures. The policy
on Academic Honesty can be accessed from the YÖK web page http://www.yok.gov.tr/web/guest/icerik/-/journal_content/56_INSTANCE_rEHF8BIsfYRx/10279/17960 Cheating in this course will result in a failing grade (F). All cheating incidents, actual or
suspected, will be reported to the Department Chair of Bioengineering and Dean of the
Faculty of Engineering. Cheating includes, but is not limited to, the following:
1. Looking at a classmate’s paper during an exam or quiz.
2. Talking to a classmate during an exam or quiz.
3. Communicating via cell phone, laptop (i.e. text messaging, talking, sending e-mails,
etc.) during an exam or quiz.
If you want an “A” in the course, you should expect to:
1. Attend all classes, take good notes during the class, and do well on the graded in-class
exercises. You may choose to use the textbook for the class to take notes, since many
of the powerpoint presentations during lecture will include figures directly from the
book chapters.
2. Sit in the front of the class where you can see, hear and engage with the professor.
Sitting in the back exposes you to disruptive, talking students
3. Read all assigned material. Concept-map each chapter. Master the vocabulary for
each topic.
4. Prepare for the mid-term exams by anticipating questions. Instructors always give
clues as to what they think are important concepts during the class. If you come to
class late or don’t engage your attention, you will miss this information.
5. Carry out all the assignments with excellent material, significant time commitment,
and good writing. Insist on reviewing the assignment before it is submitted and be
sure it is the best you can do.
6. Carry out all the assignments with excellent material, significant time commitment,
and good writing. Insist on reviewing the assignment before it is submitted and be
sure it is the best you can do.
General Microbiology Lab
BIE 2127, Fall 2019 Page | 6
Date Groups Tentative Course Content
Sep 25 Lecture in classroom
Oct 2 Group 1 Module 1. Laboratory Safety Rules, Microbiological Culture Media Preparation and Sterilization &
Collection of Environmental Samples, Culture Transfer Instruments, Techniques, and Isolation and
Maintenance of Pure Cultures – Streak-plate Technique, Spread-Plate Technique Oct 9 Group 2
Oct 16 Group 1 Module 2. Smear Preparation & Gram Staining & Bright-Field Light Microscope
Oct 23 Group 2
Oct 30 Group 1 Module 3. Effect of Temperature on Mesophilic Bacteria Growth using Pour-Plate Technique
Nov 6 Group 2
Mid-term Exams Mid-term Exams (Exam date will be announced)
Nov 20 Group 1 Module 4. Serial Dilution & Agar Plate Technique to Quantitate Viable Cells
Nov 27 Group 2
Dec 4 Group 1 Module 5. The Effects of Chemical Agents on Bacteria I: Disinfectants
Determination of MIC using broth tube dilution method Dec 11 Group 2
Dec 18 Group 1 Module 6. The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents
Determination of MIC using Kirby-Bauer Disc Diffusion Susceptibility Test Dec 25 Group 2
Final Exam (Exam date will be announced)
2019-2020 Fall, BIE 2127 General Microbiology, BIE 2101 Microbiology Lab. Groups
Group I Group II
Std. No Name/Surname
Std. No Name/Surname
150323003 Ülkü BALANDIZ
150323016 Hasan İŞBİLİR
180323051 Doğancan ERTUGRUL
180323037 Simay ÖZVAROL
130323003 Memiş Bünyamin DAĞLI
140323001 Elif Canay BOZOĞLU
150323020 Necla Merve OĞUR
160323003 Meltem YAĞCIOĞLU
180323034 Oğuzhan GÜLER
180323018 Elif HARDURA
140323025 Melis ULUCAMKIRAN
140323028 Özge TOPCU
160323005 Eda NAS
160323010 Temmuz Ege KAPLAN
170323054 Merve Yağmur UZAM
170323053 Aslıhan ŞAHİN
140323039 Hande ÖZDEMİR
160323012 Ayşenur KESİMCİ
160323021 Elif Şifa ATAMAN
160323023 Dilara SABIRTAŞ
170323049 Yeşim DEMİRCİ
170323048 Yasemin EKELİK
160323029 Ezgi Dinay AKANSOY
160323032 Egemen İLHAN
170323047 Yağmur Doğa LEVENT
170323046 Yağız ALEMDAR
160323033 Tuğba BODUR
160323034 Taha UÇAR
170323043 Süeda Betül TAŞKIN
170323041 Sıla KAZAKLI
160323036 Gürkan Ali KAPSIZLAR
160323040 Bilal ÖZEN
170323040 Sezen DAĞDEVİREN
170323039 Semah KORKMAZ
170323003 Aleyna ÖĞRETEN
170323004 Aleyna FİLİZFİDANOĞLU
170323038 Pınar Biray KIRTAŞ
170323036 Özlem EREZ
170323005 Ali KARAKAŞ
170323006 Arda HANCI
170323034 Nisanur POLAT
170323033 Nil SAZLI
170323008 Ayşe Nur SARIYILDIZ
170323010 Burcu UGRAL
170323031 Melisa KAYA
170323030 Kardelen HOROZ
170323011 Büşra AYHAN
170323028 Gülcan PORTAKAL
170323012 Büşra LULACİ
170323013 Büşra ARSLAN
170323027 Fırat Ege ÇALIŞKAN
170323026 Esma Nur BEYTUR
170323014 Büşranur ULUPINAR
170323015 Cansu ERSÖZ
170323025 Emine Ecem KAŞIKÇI
170323024 Elif YAVŞAN
170323016 Ceylin KÖYMEN
170323020 Didem Seçil ERTAN
170323023 Ekinsu YILMAZOK
170323022 Ecem GİLİTCİ
170323021 Dilara PEHLİVAN
T.C.
MANISA CELAL BAYAR UNIVERSITY
BIOENGINEERING DEPARTMENT
…….. LABORATORY REPORT
EXPERIMENT ….
NAME OF EXPERIMENT
REPORT …
Name-SURNAME
Lecturer
Name SURNAME
Laboratory Assistants
Name SURNAME
MANISA, 2019
İçindekiler ABSTRACT ................................................................................................................................................ 3
1. INTRODUCTION ............................................................................................................................... 3
1.1 General Writing Rules .................................................................................................................... 3
2. MATERIALS & METHODS ................................................................................................................. 4
2.1 Materials .................................................................................................................................. 4
2.2 Methods .................................................................................................................................. 4
2.3 General Writing Rules.............................................................................................................. 4
3. RESULTS ........................................................................................................................................... 5
4. DISCUSSION ..................................................................................................................................... 6
5. CONCLUSION ................................................................................................................................... 6
6. REFERENCES .................................................................................................................................... 6
ABSTRACT
General informations about experiment are given in this title. The purpose, scope,
method and conclusions of the experiment should be mentioned clearly and concise.
This part should be included approximately 100-150 words.
1. INTRODUCTION
A microbe, or microorganism, is a microscopic organism that comprises either a single
cell (unicellular); cell clusters; or multicellular, relatively complex organisms. The study of
microorganisms is called microbiology, a subject that began with Anton van Leeuwenhoek’s
discovery of microorganisms in 1675, using a microscope of his own design [1].
A Drawing of Microbes: This is a drawing of what Arthur Hill Hassall saw under a
microscope in a sample of water taken from the River Thames at two locations. Hassall was
able to identify many microscopic organisms not perceptible to the unaided eye [2].
Microorganisms are very diverse; they include bacteria, fungi, algae, and protozoa;
microscopic plants (green algae); and animals such as rotifers and planarians [1,3].
Objective of this experiment ……….
1.1 General Writing Rules
It can be written on both sides of the paper.
The text should lean on both sides and margin settings should be done. ,
Pages should be numbered. Add→Page Number
Passive voice should be used during writing.
“Times New Roman 12p” should be used and 13 punto-bold characters can be used for
first degree titles and 12 punto-bold characters can be used for other degree titles.
Paragraphs should be started from 1 cm inside.
References must be specified at the end of the sentence. (You can look at the internet how
to write references in the sentences).
Introduction part should be written approximately 1 page and objective of experiment
should be expressed in this section.
2. MATERIALS & METHODS
2.1 Materials
The materials which is used in this experiment are shown in below.
- Microscope (figure 1.a)
- Glass tubes (figure 1.b)
- Agar plate
Figure 1. (a) Microscope (b) Glass tube
2.2 Methods
In recent years automated cell counting has become an attractive alternative to manual
hemocytometer-based cell counting, offering more reliable results in a fraction of the time
needed for manual counting.
2.3 General Writing Rules
In this text, only materials were used and methods should be written. Obtained results,
graphs, figures, photos should be put and examined in results part and these results’
interpretations and comparisons should be explained in discussion part.
You can write them as text or as items.
Before the figures or tables, description must be made in text.
There must be a description of table or figure. This description should be written before
the table and after the figure.
Table and figure sizes must not be too large.
These rules should be used in “Methods, Results and Discussion”
3. RESULTS
Maximum values of growth parameters obtained at the stationary growth phase of the
strains were used for statistical calculation and for interpretation of the data. Temperature had
a significant (P B 0.05) effect on all growth parameters (OD600, viable counts, and dry mass)
for each strain. For the bacteria P. heparinus, P. piscium and P. cryoconitis, maximum
OD600 values were obtained at 20, 1–20, and 10C, respectively (Fig. 1, Table 2).
Figure 2. ……
Table 1. ……………..
4. DISCUSSION
All growth parameters measured in this study indicate that psychrophiles, i.e.
microorganisms able to grow well around 1C, are well adapted to low temperatures, as
demonstrated by high values for biomass formation at this temperature. The results obtained
in this study clearly show that the maximum growth temperature or the temperature at which
microbial growth is fastest (often named the ‘‘optimum temperature’’) was in no case (as
tested with three bacterial and three yeast strains) the temperature at which strains produced
the highest amount of biomass, as determined with OD600, viable counts and cell dry mass.
On the contrary, especially psychrophiles exhibited best performance in terms of biomass
production at substantially lower temperatures, i.e. at a temperature lower by 20C than the so-
called optimum ‘‘growth temperature’’. The same effect was noted for mesophilic strains
(biomass production was highest at temperatures lower by ca. 10C than the maximum growth
temperature), although to a lower extent than observed with psychrophiles.
5. CONCLUSION
All growth parameters measured in this study indicate that psychrophiles, i.e.
microorganisms able to grow well around 1C, are well adapted to low temperatures, as
demonstrated by high values for biomass formation at this temperature. The results obtained
in this study clearly show that the maximum growth temperature or the temperature at which
microbial growth is fastest (often named the ‘‘optimum temperature’’) was in no case (as
tested with three bacterial and three yeast strains) the temperature at which strains produced
the highest amount of biomass, as determined with OD600, viable counts and cell dry mass.
6. REFERENCES
Yeşilada, E., 1978, Kış koşullarında inşaat, Bayındırlık İşleri Dergisi, 44(21):31-38 s.
(tek yazarlı makale)
Acar, N. ve Estaş, S., 1986, Kesikli Seri Üretim Sistemlerinde Planlama ve Kontrol
Çalışmaları, MPM Yayınları, 309, Ankara, 356s. (one author articles)
Akal, Z., et al., 1985, İnşaat Endüstrisinde Verimlilik ve İş Etüdü Önemi ve Kullanımı,
Milli Prodüktivite Merkezi Yayınları, 317, Ankara, 156s. (two or more author articles)
Bursal, N., 1968, Maliyet Muhasebesi Prensipleri ve Tatbikatı, Yalkın Of-set, İstanbul,
442s. (one author books)
Bayındırlık Bakanlığı, 1974, Yapı İşleri Birim Fiat Tarifleri, Bayındırlık Bakanlığı
Personeli Biriktirme ve Yardımlaşma Sandığı, 47, Ankara, 156s. (official sources)
Ören, T. 1998. “Bilişimde Özenli Türkçe”,
http://www.site.uottawa.ca/~oren/pubs/pubs1998/pubs-1998-03-BOT.pdf (Date accessed:
03.03 2009) (web sources)